Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,978,585	A→C	I102M (ATT→ATG)	unknown ←	RTX toxin transporter, ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,978,585	0	A	C	100.0%	159.8 / NA	53	I102M (ATT→ATG)	unknown	RTX toxin transporter, ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/22);  total (31/22)

GAACCAGGACTTTATCCATCACGACTTGAAAAAAGAGTGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACGGCAGGAATAAACCAGGTAAAGTCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGATGCAATTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGAATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTTAATAAAATAAAGTGTTCACCATCATCACGCCAAGCCAATGCCGGTAAATGTAAAAAAGGCAATCTGTCAACTGTTTTGTTCGCAGTTCTTACCTTTAATCCAAGTGATTTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCCGGGTTAATTGAAATATTATGA  >  1/1978338‑1978831                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       gAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCAtt                                                                                                                                                                                                                                                     >  1:333185/1‑251 (MQ=255)   aCCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAg                                                                                                                                                                                                                                                   >  1:683547/1‑251 (MQ=255)     caagaccttATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGAt                                                                                                                                                                                                                                                 <  1:314564/244‑1 (MQ=255)           cttATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCaaaaa                                                                                                                                                                                                                                           <  1:497234/250‑1 (MQ=255)                        aCTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGAt                                                                                                                                                                                                                               <  1:72530/250‑1 (MQ=255)                         cTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCg                                                                                                                                                                                                                             >  1:414826/1‑251 (MQ=255)                            gAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGtt                                                                                                                                                                                                                          >  1:83480/1‑251 (MQ=255)                                       ggtgTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTAt                                                                                                                                                                                                               >  1:538728/1‑251 (MQ=255)                                        gtgtAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTAtt                                                                                                                                                                                                              >  1:656098/1‑251 (MQ=255)                                        gtgtAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTAtt                                                                                                                                                                                                              >  1:252514/1‑251 (MQ=255)                                                 gTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAgg                                                                                                                                                                                                                                      <  1:343534/218‑1 (MQ=255)                                                 gTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCaataaa                                                                                                                                                                                                     >  1:849964/1‑251 (MQ=255)                                                 gTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCaataaa                                                                                                                                                                                                     >  1:681118/1‑251 (MQ=255)                                                 gTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCaataaa                                                                                                                                                                                                     >  1:686088/1‑251 (MQ=255)                                                     aaaaaTTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCaataaaataa                                                                                                                                                                                                 <  1:567909/251‑1 (MQ=255)                                                          tttGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAgtgg                                                                                                                                                                                            <  1:195016/251‑2 (MQ=255)                                                                            cGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACgccaa                                                                                                                                                                          >  1:885193/1‑251 (MQ=255)                                                                                     tttCTATAAAAATTTTGCGGTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAAtac                                                                                                                                                                  <  1:93367/250‑3 (MQ=255)                                                                                                        gTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATg                                                                                                                                                                                                                                         >  1:600105/1‑160 (MQ=255)                                                                                                        gTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGtt                                                                                                                                                                                                                          >  1:472839/1‑175 (MQ=255)                                                                                                        gTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACgccaagcc                                                                                                                                                                       <  1:481532/226‑1 (MQ=255)                                                                                                        gTATTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAggta                                                                                                                                              >  1:431418/1‑249 (MQ=255)                                                                                                          aTTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACgccaagc                                                                                                                                                                        >  1:571277/1‑223 (MQ=255)                                                                                                          aTTTAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAAtac                                                                                                                                                                  <  1:618817/229‑3 (MQ=255)                                                                                                             tAATTACAGCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTcaat                                                                                                                                                                                                        >  1:778095/1‑188 (MQ=255)                                                                                                                   cagCAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTg                                                                                                                                                                                                                     >  1:322745/3‑169 (MQ=255)                                                                                                                      cAGGAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGt                                                                                                                                 <  1:570325/250‑1 (MQ=255)                                                                                                                        ggAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAgt                                                                                                                                                                                              >  1:472245/1‑187 (MQ=255)                                                                                                                        ggAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGtttt                                                                                                                              >  1:541494/1‑251 (MQ=255)                                                                                                                         gAATAAACCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAAtac                                                                                                                                                                  <  1:814704/214‑3 (MQ=255)                                                                                                                              aaCCAGGTAAAATCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCaataaaat                                                                                                                                                                                                   >  1:847325/1‑176 (MQ=255)                                                                                                                                        aatCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTa                                                                                                                >  1:636608/3‑249 (MQ=255)                                                                                                                                                           tCCTACAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCaataaaataa                                                                                                                                                                                                 >  1:481947/1‑149 (MQ=255)                                                                                                                                                                cAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTcaat                                                                                                                                                                                                        <  1:58147/137‑1 (MQ=255)                                                                                                                                                                cAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAgtgg                                                                                                                                                                                            <  1:532995/149‑2 (MQ=255)                                                                                                                                                                cAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATc                                                                                                       >  1:19746/1‑234 (MQ=255)                                                                                                                                                                cAATAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTtgctg                                                                                        <  1:339897/249‑1 (MQ=255)                                                                                                                                                                  aTAGATGCGCGTGACGCAACTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGAAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAggt                                                                                                                                               >  1:569858/1‑191 (MQ=255)                                                                                                                                                                                         aaTCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAgcc                                                             <  1:183974/251‑1 (MQ=255)                                                                                                                                                                                          aTCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAgccc                                                            <  1:622500/251‑1 (MQ=255)                                                                                                                                                                                               cTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAgcccatgcc                                                       <  1:217913/251‑1 (MQ=255)                                                                                                                                                                                                 tGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAAtac                                                                                                                                                                  <  1:331920/142‑3 (MQ=255)                                                                                                                                                                                                  gCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCt                                                                                                                                          <  1:505711/165‑1 (MQ=255)                                                                                                                                                                                                                cttcAAATTCA‑TTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTAt                                     >  1:611760/1‑251 (MQ=255)                                                                                                                                                                                                                         cATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAAtacc                                                                                                                                                                 >  1:197240/1‑116 (MQ=255)                                                                                                                                                                                                                         cATTTTTGTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTAtt                             >  1:875279/1‑251 (MQ=255)                                                                                                                                                                                                                                gTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTAct                                                                                                              <  1:400639/163‑2 (MQ=255)                                                                                                                                                                                                                                gTCTAAAACAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTc                                                                 >  1:313598/1‑208 (MQ=255)                                                                                                                                                                                                                                     aaaCAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAgcccatgcc                                                       >  1:148087/1‑213 (MQ=255)                                                                                                                                                                                                                                        cAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATc                                                                                                       >  1:348643/1‑162 (MQ=255)                                                                                                                                                                                                                                        cAATAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAAtt              >  1:379943/1‑251 (MQ=255)                                                                                                                                                                                                                                         aaTAGGATTTTTTTGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTg             <  1:28126/251‑1 (MQ=255)                                                                                                                                                                                                                                                    tttGCATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTCAATAAAATAAAGTGGTCACCATCATCACGCCAAGCCAATACCGGTAAATGTAAAAAAGGTAATCTATCAATTGTTTTGTTCGTAGTTCTTACTTTTAATCCAAGTGATCTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCAGGGTTAATTGAAATATTATGa  <  1:456801/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       GAACCAGGACTTTATCCATCACGACTTGAAAAAAGAGTGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAAAATTTTGCGGTATTTAATTACGGCAGGAATAAACCAGGTAAAGTCGAATTTTGCTAAATTTCCTACAATAGATGCGCGTGATGCAATTAAAATCACCTTGCTTTGATAACGTTCTTCAAATTCATTTTTGTCTAAAACAATAGGATTTTTTTGAATTAGATCAAAAATGAGGTAACGATCGGTTTCTTGATCTATTTTTAATAAAATAAAGTGTTCACCATCATCACGCCAAGCCAATGCCGGTAAATGTAAAAAAGGCAATCTGTCAACTGTTTTGTTCGCAGTTCTTACCTTTAATCCAAGTGATTTTGCTGCAAGCAACCATTTAGTTTGGTTCAGCCCATGCCCATCTATATCAAATTTATGTTTTATTTCTTCCGGGTTAATTGAAATATTATGA  >  1/1978338‑1978831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑