Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	297,692	A→G	C227R (TGT→CGT)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	297,692	0	A	G	100.0%	173.5 / NA	61	C227R (TGT→CGT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/25);  total (36/25)

ATCCTACTATGTGGATTACATATGGCTGCTACATACCTAAACATGTCATTCTGCCCCTTAGGAATTACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACACACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCATCAAGCCAATTTCTTGCTATCCAAGACGCCGTTTTATAACCAATTCCTTGTATTTGTAATAACCAATTTCTTAATTGTTTACCATTACCAAATGG  >  1/297450‑297929                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               aTCCTACTATGTGGATTACATATGGCTGCTACATACCTAAACATGTCATTCTGCCCCTTAGGAATTACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCaa                                                                                                                                                                                                                                        >  1:672959/1‑251 (MQ=255) aTCCTACTATGTGGATTACATATGGCTGCTACATACCTAAACATGTCATTCTGCCCCTTAGGAATTACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCaa                                                                                                                                                                                                                                        >  1:876900/1‑251 (MQ=255)   ccTACTATGTGGATTACATATGGCTGCTACATACCTAAACATGTCATTCTGCCCCTTAGGAATTACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGt                                                                                                                                                                                                                                      >  1:673042/1‑251 (MQ=255)    ctactaTGTGGATTACATATGGCTGCTACATACCTAAACATGTCATTCTGCCCCTTAGGAATTACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTg                                                                                                                                                                                                                                     >  1:761683/1‑251 (MQ=255)    ctactaTGTGGATTACATATGGCTGCTACATACCTAAACATGTCATTCTGCCCCTTAGGAATTACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTg                                                                                                                                                                                                                                     <  1:525701/251‑1 (MQ=255)           gtgGATTACATATGGCTGCTACATACCTAAACATGTCATTCTGCCCCTTAGGAATTACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGc                                                                                                                                                                                                                                    >  1:510099/1‑245 (MQ=255)                            gctACATACCTAAACATGTCATTCTGCCCCTTAGGAATTACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTg                                                                                                                                                                                                                                     >  1:573136/1‑227 (MQ=255)                                  tACCTAAACATGTCATTCTGCCCCTTAGGAATTACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTa                                                                                                                                                                                                       <  1:247868/251‑1 (MQ=255)                                  tACCTAAACATGTCATTCTGCCCCTTAGGAATTACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTa                                                                                                                                                                                                       >  1:882619/1‑251 (MQ=255)                                    ccTAAACATGTCATTCTGCCCCTTAGGAATTACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTaa                                                                                                                                                                                                      >  1:204820/1‑250 (MQ=255)                                    ccTAAACATGTCATTCTGCCCCTTAGGAATTACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAc                                                                                                                                                                                                     <  1:693794/251‑1 (MQ=255)                                    ccTAAACATGTCATTCTGCCCCTTAGGAATTACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATAATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTggg                                                                                                                                                                                                                               <  1:316894/225‑1 (MQ=255)                                                                aTTACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAAt                                                                                                                                                                        <  1:10801/251‑1 (MQ=255)                                                                 ttACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAAtt                                                                                                                                                                       <  1:767171/251‑1 (MQ=255)                                                                 ttACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAAtt                                                                                                                                                                       <  1:242472/251‑1 (MQ=255)                                                                                        aaaCTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTAccc                                                                                                                                                <  1:565229/251‑1 (MQ=255)                                                                                           cTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAg                                                                                                                                                                                                                                       >  1:306587/1‑162 (MQ=255)                                                                                           cTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTg                                                                                                                                                                                                                                     >  1:118592/1‑164 (MQ=255)                                                                                            tATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAg                                                                                                                                                                                                                                       >  1:416258/1‑161 (MQ=255)                                                                                                         tAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAatata                                                                                                                               <  1:116677/251‑1 (MQ=255)                                                                                                         tAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAatata                                                                                                                               <  1:785084/251‑1 (MQ=255)                                                                                                             ttgtttgGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGa                                                                                                                           <  1:138357/251‑1 (MQ=255)                                                                                                                    gAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAAtt                                                                                                                                                                       <  1:169876/200‑1 (MQ=255)                                                                                                                       tGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTTAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGa                                                                                                                  <  1:430259/250‑1 (MQ=255)                                                                                                                                     ttCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAg                                                                                                   <  1:527422/251‑1 (MQ=255)                                                                                                                                         gTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTGCCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATAATCAGCAt                                                                                                <  1:829519/250‑1 (MQ=255)                                                                                                                                         gTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTc                                                                                                                                             >  1:87977/1‑205 (MQ=255)                                                                                                                                         gTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAatata                                                                                                                               >  1:270193/1‑219 (MQ=255)                                                                                                                                                   tGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCATCAAGCCAAttt                                                                                     <  1:21579/251‑1 (MQ=255)                                                                                                                                                      cccATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAg                                                                                                   >  1:147973/1‑234 (MQ=255)                                                                                                                                                           gATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAAt                                                                                                                                                                        >  1:790618/1‑160 (MQ=255)                                                                                                                                                           gATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAg                                                                                                                                                                     >  1:700932/1‑163 (MQ=255)                                                                                                                                                           gATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTAccc                                                                                                                                                <  1:338189/184‑1 (MQ=255)                                                                                                                                                              aGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCAt                                                                                                >  1:96252/1‑229 (MQ=255)                                                                                                                                                               gTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAatata                                                                                                                               >  1:511813/1‑197 (MQ=255)                                                                                                                                                               gTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATgg                                                                                                                            <  1:791183/200‑1 (MQ=255)                                                                                                                                                                       gTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGAc                                                                                                                          >  1:794106/1‑194 (MQ=255)                                                                                                                                                                              ttctttTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCATCAAGCCAATTTCTTGCTATCCAAGACGCCGTTTTATaa                                                          >  1:96077/1‑251 (MQ=255)                                                                                                                                                                                     tCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTc                                                                                                                                                                                                                        >  1:545098/1‑87 (MQ=255)                                                                                                                                                                                       tAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAGCTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCATCAAGCCAATTTCTTGCTATCCAAGACGCCGTTTTATAACCAATTCCt                                                 <  1:870839/251‑1 (MQ=255)                                                                                                                                                                                           tatatTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTcc                                                                                                                                            >  1:532771/1‑156 (MQ=255)                                                                                                                                                                                            atatTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAACTTTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATg                                                                                                                             >  1:61801/1‑171 (MQ=255)                                                                                                                                                                                                  gttgtCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAAc                                                                                                                                                          >  1:486367/1‑135 (MQ=255)                                                                                                                                                                                                         gCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCATCAAGCCAATTTCTTGCTATCCAAGACGCCGTTTtat                                                            <  1:174430/222‑1 (MQ=255)                                                                                                                                                                                                         gCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCATCAAGCCAATTTCTTGCTATCCAAGACGCCGTTTTATAACCAATTCCTTGTATTTGTAATAACCaa                               >  1:381066/1‑251 (MQ=255)                                                                                                                                                                                                          catcatATCATACCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCATCAAGCCAATTTCTTGCTATCCAAGACGCCGTTTTATAACCAATTCCTTGTATTTGTAATAACCAAt                              >  1:240962/1‑251 (MQ=255)                                                                                                                                                                                                              atatcataCCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAg                                                                                                                                                                     >  1:396992/1‑112 (MQ=255)                                                                                                                                                                                                              atatcataCCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAat                                                                                                                                  >  1:785146/1‑147 (MQ=255)                                                                                                                                                                                                                   ataCCAAATAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCATCAAGCCAATTTCTTGCTATCCAAGACGCCGTTTTATAACCAATTCCTTGTATTTGTAATAACCAATTTCTTAAtt                     >  1:225653/1‑251 (MQ=255)                                                                                                                                                                                                                        aaaTAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGa                                                                                                                  >  1:846168/1‑153 (MQ=255)                                                                                                                                                                                                                        aaaTAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCATCAAGCCAATTTCTTGCTATCCAAGACGCCGTTTTATAAc                                                         >  1:44194/1‑210 (MQ=255)                                                                                                                                                                                                                        aaaTAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCATCAAGCCAATTTCTTGCTATCCAAGACGCCGTTTTATAACCAATTCCTTGTa                                             <  1:897968/222‑1 (MQ=255)                                                                                                                                                                                                                        aaaTAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCATCAAGCCAATTTCTTGCTATCCAAGACGCCGTTTTATAACCAATTCCTTGTATTTGTAATAACCAATTTCTTAATTGTtta                >  1:602987/1‑251 (MQ=255)                                                                                                                                                                                                                        aaaTAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCATCAAGCCAATTTCTTGCTATCCAAGACGCCGTTTTATAACCAATTCCTTGTATTTGTAATAACCAATTTCTTAATTGTtta                >  1:138556/1‑251 (MQ=255)                                                                                                                                                                                                                        aaaTAGTAGCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCAAAGATAGCAACATCATCAGCATCAAGCCAATTTCtt                                                                                  >  1:73004/1‑185 (MQ=255)                                                                                                                                                                                                                           tagtagCATCCAATTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCATCAAGCCAATTTCTTGCTATCCAAGACGCCGTTTTATAACCAATTCCTTGTATTTGTAATAACCAATTTCTTAATTGTttacca             <  1:794113/251‑1 (MQ=255)                                                                                                                                                                                                                                       aTTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGAc                                                                                                                          <  1:810300/130‑1 (MQ=255)                                                                                                                                                                                                                                       aTTCTGAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCATCAAGCCAATTTCTTGCTATCCAAGACGCCGTTTTATAACCAATTcc                                                  >  1:205531/1‑202 (MQ=255)                                                                                                                                                                                                                                            gAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAatata                                                                                                                               <  1:201006/120‑1 (MQ=255)                                                                                                                                                                                                                                            gAAGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCATCAAGCCAATTTCTTGCTATCCAAGACGCCGTTTTATAACCAATTCCTTGTATTTGTAATAACCAATTTCTTAATTGTTTACCATTACCAAAtgg  <  1:859204/245‑1 (MQ=255)                                                                                                                                                                                                                                              aGTACGCACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAatata                                                                                                                               >  1:706435/1‑118 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               ATCCTACTATGTGGATTACATATGGCTGCTACATACCTAAACATGTCATTCTGCCCCTTAGGAATTACTGGCGAGCCATGGGTAAGCAAACTATCAGAACAATCTAATTTGTTTGGAGTGGGAGTTGCTATTTTCGGTAGAAATACTGTCCCATGATAGTGCCTGAGTACTCTTTCTTTTTCTAAATATATTTGTTGTCAGCATCATATCATACCAAATAGTAGCATCCAATTCTGAAGTACACACCCCAAGTGCTTGGGCAAATTCTAAAAACTGCTCCTCTAAC‑TTTAAATAATTTTTTTCAACTGTTAAATTAGTATCAAAAAACTTCGCTACCCTTCCCGCTCTTAATATATGGACATCTAAGATAGCAACATCATCAGCATCAAGCCAATTTCTTGCTATCCAAGACGCCGTTTTATAACCAATTCCTTGTATTTGTAATAACCAATTTCTTAATTGTTTACCATTACCAAATGG  >  1/297450‑297929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑