Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,978,189	A→G	H234H (CAT→CAC)	unknown ←	RTX toxin transporter, ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,978,189	0	A	G	100.0%	232.3 / NA	77	H234H (CAT→CAC)	unknown	RTX toxin transporter, ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/43);  total (34/43)

AATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATTACAGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAACTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAATGACGAAAAAGCCTTGCTCCCAATTCGACATCAATACGACTTGTACTATGAGCAAAAACATAGGTTCTCAAGCCTCCCAAGATTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATTACATTCAATGTTGAAAATCCACGGTGAACCAGGACTTTATCCATCACGACTTGAAAAAAGAGTGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAA  >  1/1977958‑1978431                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   aataaAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCt                                                                                                                                                                                                                                  >  1:533878/1‑250 (MQ=255) aataaAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCt                                                                                                                                                                                                                                  >  1:639711/1‑250 (MQ=255) aataaAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCt                                                                                                                                                                                                                                  >  1:210169/1‑250 (MQ=255) aataaAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTc                                                                                                                                                                                                                                 <  1:852227/251‑1 (MQ=255)             ccAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACAt                                                                                                                                                                                                                     <  1:421804/251‑1 (MQ=255)             ccAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACAt                                                                                                                                                                                                                     <  1:763013/251‑1 (MQ=255)                    aCATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACg                                                                                                                                                                                                              >  1:633345/1‑251 (MQ=255)                             ggTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGtg                                                                                                                                                                                                       >  1:107874/1‑248 (MQ=255)                              gTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGAc                                                                                                                                                                                                                       <  1:228556/232‑1 (MQ=255)                              gTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTa                                                                                                                                                                                                    >  1:715704/1‑251 (MQ=255)                              gTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTa                                                                                                                                                                                                    >  1:717705/1‑251 (MQ=255)                              gTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTa                                                                                                                                                                                                    >  1:290539/1‑251 (MQ=255)                                   gAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAg                                                                                                                                                                                                >  1:416262/1‑250 (MQ=255)                                             cAACTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGtgct                                                                                                                                                                                                     <  1:501855/235‑4 (MQ=255)                                              aaCTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAgg                                                                                                                                                                                    <  1:777333/251‑1 (MQ=255)                                               aCTAATGTTAATTTTGGGCTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTATAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTGCGAATTTGATCTAATACACGAACACGAGCCACCGTATCCCCAAGCCGACGAGCTTCAAAATAAGAAATCGGTAAAACTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGt                                                                                                                                                                                   <  1:616305/251‑1 (MQ=255)                                                                 cTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGt                                                                                                                                                                                   >  1:719673/1‑233 (MQ=255)                                                                 cTGTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGac                                                                                                                                                                 <  1:761572/251‑2 (MQ=255)                                                                   gTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACAt                                                                                                                                                                                       <  1:133972/227‑1 (MQ=255)                                                                   gTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGacta                                                                                                                                                               >  1:507986/1‑248 (MQ=255)                                                                   gTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGacta                                                                                                                                                               >  1:739869/1‑248 (MQ=255)                                                                   gTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGacta                                                                                                                                                               >  1:406006/1‑248 (MQ=255)                                                                   gTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGac                                                                                                                                                                 <  1:201054/249‑2 (MQ=255)                                                                   gTAATACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGac                                                                                                                                                                 <  1:670742/249‑2 (MQ=255)                                                                      aTACCACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTAttt                                                                                                                                                            >  1:83799/1‑251 (MQ=255)                                                                         ccACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCaa                                                                                                                                                         >  1:61930/1‑251 (MQ=255)                                                                         ccACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCaa                                                                                                                                                         >  1:58880/1‑251 (MQ=255)                                                                         ccACATAACGGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCaa                                                                                                                                                         <  1:247381/251‑1 (MQ=255)                                                                           acataacggCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAAt                                                                                                                                                       <  1:724270/243‑1 (MQ=255)                                                                           acataacggCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAAt                                                                                                                                                       <  1:297267/243‑1 (MQ=255)                                                                                          aaaTAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCa                                                                                                                                        <  1:761232/251‑1 (MQ=255)                                                                                                 agagAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGAc                                                                                                                                                                                                            <  1:385979/176‑1 (MQ=255)                                                                                                   agaATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCa                                                                                                                                 >  1:838712/1‑249 (MQ=255)                                                                                                                   aaTAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATg                                                                                                               <  1:424440/251‑1 (MQ=255)                                                                                                                    aTAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAAtca                                                                                                                        <  1:333660/241‑3 (MQ=255)                                                                                                                    aTAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGt                                                                                                              <  1:733920/251‑1 (MQ=255)                                                                                                                         ggTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGaaa                                                                                                         >  1:215866/1‑251 (MQ=255)                                                                                                                         ggTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGaaa                                                                                                         >  1:756074/1‑251 (MQ=255)                                                                                                                         ggTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGaaa                                                                                                         >  1:172304/1‑251 (MQ=255)                                                                                                                          gTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGaaaa                                                                                                        >  1:370690/1‑251 (MQ=255)                                                                                                                              gTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTtc                                                                                                                                                                                 <  1:362437/174‑1 (MQ=255)                                                                                                                              gTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGac                                                                                                                                                                 <  1:288021/190‑2 (MQ=255)                                                                                                                              gTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCaa                                                                                                                                       <  1:325042/216‑1 (MQ=255)                                                                                                                              gTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATg                                                                                                               <  1:890129/240‑1 (MQ=255)                                                                                                                              gTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCa                                                                                                    >  1:238974/1‑251 (MQ=255)                                                                                                                                        tGTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCa                                                                                          <  1:290346/251‑1 (MQ=255)                                                                                                                                         gTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACTTCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGt                                                                                                              <  1:365951/230‑1 (MQ=255)                                                                                                                                         gTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGtgc                                                                                                                                                                                                      <  1:27486/142‑3 (MQ=255)                                                                                                                                         gTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAg                                                                                                                                          <  1:421191/202‑1 (MQ=255)                                                                                                                                         gTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACAt                                                                                                                     <  1:402946/223‑1 (MQ=255)                                                                                                                                         gTTAAGAAATTCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCaa                                                                                         >  1:51621/1‑250 (MQ=255)                                                                                                                                                  ttCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGAcc                                                                                     >  1:41838/1‑245 (MQ=255)                                                                                                                                                   tCCGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAGTAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATcc                                                                               <  1:596756/251‑1 (MQ=255)                                                                                                                                                    ccGAATTTGATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCa                                                                              <  1:250833/251‑1 (MQ=255)                                                                                                                                                            gATCTAATTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGACTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGa                                                                        >  1:619256/1‑249 (MQ=255)                                                                                                                                                             aTCTAATTCACGAACACGAGCCATCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCTAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACtt                                                                     >  1:709014/1‑251 (MQ=255)                                                                                                                                                                          acacGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAAt                                                                                                                                            >  1:158496/1‑167 (MQ=255)                                                                                                                                                                             cGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGgtgt                                                     <  1:836711/251‑1 (MQ=255)                                                                                                                                                                              gAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATcc                                                                               <  1:84237/224‑1 (MQ=255)                                                                                                                                                                              gAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAag                                                             >  1:205949/1‑242 (MQ=255)                                                                                                                                                                                 ccACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCa                                                                                                                                        >  1:257310/1‑164 (MQ=255)                                                                                                                                                                                 ccACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAAt                                                  >  1:56748/1‑250 (MQ=255)                                                                                                                                                                                 ccACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATc                                                 <  1:67996/251‑1 (MQ=255)                                                                                                                                                                                 ccACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATc                                                 <  1:555804/251‑1 (MQ=255)                                                                                                                                                                                 ccACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATc                                                 <  1:490094/251‑1 (MQ=255)                                                                                                                                                                                    ccGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGt                                              >  1:609030/1‑251 (MQ=255)                                                                                                                                                                                        aTCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATc                                                 <  1:373170/244‑1 (MQ=255)                                                                                                                                                                                         tCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGt                                                                                                                                                                                   >  1:599821/1‑113 (MQ=255)                                                                                                                                                                                         tCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAg                                                                                                                                          >  1:805201/1‑154 (MQ=255)                                                                                                                                                                                         tCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGAcc                                                                                     <  1:439406/207‑2 (MQ=255)                                                                                                                                                                                          ccccAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGac                                                                                                                                                                 <  1:462485/130‑2 (MQ=255)                                                                                                                                                                                           cccAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCaaaaa                                       <  1:391527/251‑1 (MQ=255)                                                                                                                                                                                              aaCCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATc                                                 <  1:375627/238‑1 (MQ=255)                                                                                                                                                                                                                                gCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTg                                                                                                                              <  1:693564/127‑1 (MQ=255)                                                                                                                                                                                                                                gCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATc                                                                                <  1:529010/173‑1 (MQ=255)                                                                                                                                                                                                                                gCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGaaa                     <  1:301673/232‑1 (MQ=255)                                                                                                                                                                                                                                gCTAATAAGTGACGAAAAAGTCTTGCTCCCAGTTCGACATCAATACGACTTGTGCTATGAGCAAACACATAGGTTCTCAGCCCCCCCAAGACTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATCACATTCAATGTTGAAAATCCACGGTGAACCAAGACCTTATCCATCACGACTTGAAAAAAGAGCGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATaaa  >  1:440328/1‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   AATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAATGTTAATTTTGGGCTGTAATACCACATTACAGCGAAGAAAATAAAAGAGAATAACAAGTCTAAAATAGAGGTAAGTGCCTGCCCTGTTAAGAAATTCCGAATTTGATCTAACTCACGAACACGAGCCACCGTATCCCCAACCCGACGAGCTTCAAAATAAGAAATCGGTAAAGCTAATAAATGACGAAAAAGCCTTGCTCCCAATTCGACATCAATACGACTTGTACTATGAGCAAAAACATAGGTTCTCAAGCCTCCCAAGATTATTTCAAATAAGACTACAATAGCAAGAGCCACTGTAATTACATTCAATGTTGAAAATCCACGGTGAACCAGGACTTTATCCATCACGACTTGAAAAAAGAGTGGTGTAATCAGTGCAAAAATTTGCAAAAATATAGAAACGATTAACGTTTCTATAAA  >  1/1977958‑1978431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑