Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	296,043	T→G	*329G (TGA→GGA)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	296,043	0	T	G	100.0%	181.2 / NA	59	*329G (TGA→GGA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (30/29);  total (30/29)

GCCTATTACTGTGAAACAAGAGGATATCCAAGCTTTGAGGAATTTACTAACTGGAAAAATCGAGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTTGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGCAAAGAATAAACCTGTTATTGTCTATAATGAAAATGAAAAATCAGAAGACTTAAAGATGATGGAAGGGACAGATTGTATAGTTATTAAAGACTATGTAAGTGCTATTTACCGCACCCTATGGG  >  1/295827‑296289                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       gCCTATTACTGTGAAACAAGAGGATATCCAAGCTTTGAGGAATTTACTAACTGGAAAAATCGAGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATaa                                                                                                                                                                                                                                      <  1:267198/235‑1 (MQ=255) gCCTATTACTGTGAAACAAGAGGATATCCAAGCTTTGAGGAATTTACTAACTGGAAAAATCGAGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGa                                                                                                                                                                                                                      >  1:635529/1‑251 (MQ=255) gCCTATTACTGTGAAACAAGAGGATATCCAAGCTTTGAGGAATTTACTAACTGGAAAAATCGAGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGa                                                                                                                                                                                                                      >  1:644912/1‑251 (MQ=255) gCCTATTACTGTGAAACAAGAGGATATCCAAGCTTTGAGGAATTTACTAACTGGAAAAATCGAGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGAGGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGa                                                                                                                                                                                                                      >  1:199491/1‑251 (MQ=255) gCCTATTACTGTGAAACAAGAGGATATCCAAGCTTTGAGGAATTTACTAACTGGAAAAATCGAGTACAACCCATA‑‑ACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGata                                                                                                                                                                                                                    >  1:689415/1‑251 (MQ=255)  ccTATTACTGTGAAACAAGAGGATATCCAAGCTTTGAGGAATTTACTAACTGGAAAAATCGAGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAg                                                                                                                                                                                                                       <  1:615753/249‑1 (MQ=255)   cTATTACTGTGAAACAAGAGGATATCCAAGCTTTGAGGAATTTACTAACTGGAAAAATCGAGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCt                                                                                                                                                                                                                           >  1:51654/1‑244 (MQ=255)   cTATTACTGTGAAACAAGAGGATATCCAAGCTTTGAGGAATTTACTAACTGGAAAAATCGAGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGata                                                                                                                                                                                                                    >  1:6833/1‑251 (MQ=255)        aCTGTGAAACAAGAGGATATCCAAGCTTTGAGGAATTTACTAACTGGAAAAATCGAGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGAt                                                                                                                                                                                                               <  1:106026/251‑1 (MQ=255)                 cAAGAGGATATCCAAGCTTTGAGGAATTTACTAACTGGAAAAATCGAGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGaataaat                                                                                                                                                                                                      <  1:286400/251‑1 (MQ=255)                     agGATATCCAAGCTTTGAGGAATTTACTAACTGGAAAAATCGAGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAataaataa                                                                                                                                                                                                    <  1:55736/249‑1 (MQ=255)                      ggATATCCAAGCTTTGAGGAATTTACTAACTGGAAAAATCGAGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAAtt                                                                                                                                                                                                  >  1:720933/1‑250 (MQ=255)                       gATATCCAAGCTTTGAGGAATTTACTAACTGGAAAAATCGAGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTc                                                                                                                                                                                                 <  1:294817/250‑1 (MQ=255)                                 cTTTGAGGAATTTACTAACTGGAAAAATCGAGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGatattata                                                                                                                                                                                       <  1:99682/250‑1 (MQ=255)                                                              gAGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCtat                                                                                                                                                                                                                                        <  1:326569/172‑1 (MQ=255)                                                               aGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTc                                                                                                                                                                                                 >  1:637151/1‑210 (MQ=255)                                                                     aCCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTc                                                                                                                                                                                                                                 <  1:2512/172‑1 (MQ=255)                                                                     aCCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTc                                                                                                                                                             <  1:632376/240‑1 (MQ=255)                                                                       ccATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTAttttt                                                                                                                                                >  1:20730/1‑251 (MQ=255)                                                                          tAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTAcc                                                                                                                                                                                                                            <  1:878843/172‑1 (MQ=255)                                                                             cacaTAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATgg                                                                                                                                                                                                                                   >  1:262468/1‑162 (MQ=255)                                                                               caTAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATgg                                                                                                                                                                                                                                   <  1:254671/160‑1 (MQ=255)                                                                                   gACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATa                                                                                                                                     >  1:350971/1‑250 (MQ=255)                                                                                            agaAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGc                                                                                                                            <  1:52209/250‑1 (MQ=255)                                                                                                  aaGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGCAAAGAAt                                                                                                                     >  1:531699/1‑251 (MQ=255)                                                                                                    gTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATaa                                                                                                                                                                                                                                      >  1:76157/1‑136 (MQ=255)                                                                                                       aCTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGt                                                                                                                                                                                                                                  >  1:395319/1‑137 (MQ=255)                                                                                                        cTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATg                                                                                                                                                                                                                                    <  1:892823/134‑1 (MQ=255)                                                                                                        cTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGaataaat                                                                                                                                                                                                      >  1:259449/1‑164 (MQ=255)                                                                                                          gTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAataaataa                                                                                                                                                                                                    >  1:276157/1‑164 (MQ=255)                                                                                                            atatTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATgg                                                                                                                                                                                                                                   <  1:2576/131‑1 (MQ=255)                                                                                                            atatTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATg                                                                                                                                                                                                                                    >  1:538786/1‑130 (MQ=255)                                                                                                            atatTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGAt                                                                                                                                                                                                               <  1:117655/151‑1 (MQ=255)                                                                                                            atatTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATGTTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGCAAAGAATAAACCTGTTa                                                                                                           >  1:427168/1‑251 (MQ=255)                                                                                                            atatTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGAc                                                                                                                                                       <  1:775003/207‑1 (MQ=255)                                                                                                            atatTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGCAAAGAAt                                                                                                                     >  1:790170/1‑241 (MQ=255)                                                                                                             tatTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGAt                                                                                                                                                                                                               <  1:647423/150‑1 (MQ=255)                                                                                                              atTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGa                                                                                                                                                                                                                      >  1:185083/1‑142 (MQ=255)                                                                                                                 tAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGAt                                                                                                                                                                                                               >  1:19468/1‑146 (MQ=255)                                                                                                                                   ctctTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATg                                                                                                                                                                                                                                    >  1:308878/1‑107 (MQ=255)                                                                                                                                     ctTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGatatcatat                                                                                                                                                                                      >  1:515542/1‑151 (MQ=255)                                                                                                                                     ctTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGCAAAGAATAAACCTGTTATTGTCTATAATGAAAATGAAAAAt                                                                                   >  1:585361/1‑250 (MQ=255)                                                                                                                                                   gCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTc                                                                                                                                                                                                 <  1:324255/126‑1 (MQ=255)                                                                                                                                                                aCTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGatat                                                                                                                                                                                                                   <  1:311290/95‑1 (MQ=255)                                                                                                                                                                aCTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGCAAAGAATAAAcc                                                                                                                <  1:765968/194‑1 (MQ=255)                                                                                                                                                                      aGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGCAAAGAATAAACCTGTTATTGTCTATAATGAAAATGAAAAATCAGAAGACTTAAAga                                                                    >  1:253870/1‑232 (MQ=255)                                                                                                                                                                        gTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCt                                                                                                                                 >  1:245295/1‑169 (MQ=255)                                                                                                                                                                              gagtgagATGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGCAAAGAATAAACCTGTTATTGTCTATAATGAAAATGAAAAATCAGAAGACTTAAAGATGATGGAAGGGACAGATTGTATAg                                            >  1:324578/1‑248 (MQ=255)                                                                                                                                                                                  gagaTGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTAcc                                                                                                                                                                                                                            >  1:375562/1‑68 (MQ=255)                                                                                                                                                                                   agaTGGGACTGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAg                                                                                                                                                                                                                       <  1:692071/72‑1 (MQ=255)                                                                                                                                                                                            tGATAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGCAAAGAATAAACCTGTTATTGTCTATAATGAAAATGAAAAATCAGAAGACTTAAAGATGATGGAAGGGACAGATTGTATAGTTATTAAAGACTATGTa                           <  1:313979/251‑1 (MQ=255)                                                                                                                                                                                              aTAGTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGCAAAGAATAAACCTGTTATTGTCTATAATGAAAATGAAAAATCAGAAGACTTAAAGATGATGGAAGGGACAGATTGTATAGTTATTAAAGACTATGTAAg                         <  1:899764/251‑1 (MQ=255)                                                                                                                                                                                                 gTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAAt                                                                                                                                                                                                                                     <  1:136912/44‑1 (MQ=255)                                                                                                                                                                                                 gTATTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATgg                                                                                                                                                                                                                                   <  1:7587/46‑1 (MQ=255)                                                                                                                                                                                                   aTTTTCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGCAAAGAATAAACCTGTTATTGTCTATAATGAAAATGAAAAATCAGAAGACTTAAAGATGATGGAAGGGACAGATTGTATAGTTATTAAAGACTATGTAAGTGCTa                    >  1:245545/1‑251 (MQ=255)                                                                                                                                                                                                       tCACCATATCATGAAATTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGCAAAGAATAAACCTGTTATTGTCTATAATGAAAATGAAAAATCAGAAGACTTAAAGATGATGGAAGGGACAGATTGTATAGTTATTAAAGAc                                 <  1:276782/234‑1 (MQ=255)                                                                                                                                                                                                                     aaTTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGCaa                                                                                                                          >  1:565290/1‑131 (MQ=255)                                                                                                                                                                                                                     aaTTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGCAAAGAATAAACCTGTTATTGTCTATAATGAAAATGAAAAATCAGAAGACTTAAAGATGATGGAAGGGACAGATTGTATAGTTATTAAAGACTATGTAAGTGCTATTTACCGCACCCTATggg  <  1:830657/251‑1 (MQ=255)                                                                                                                                                                                                                     aaTTGGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGCAAAGAATAAACCTGTTATTGTCTATAATGAAAATGAAAAATCAGAAGACTTAAAGATGATGGAAGGGACAGATTGTATAGTTATTAAAGACTATGTAAGTGCTATTTACCGCACCCTATggg  <  1:463534/251‑1 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       GCCTATTACTGTGAAACAAGAGGATATCCAAGCTTTGAGGAATTTACTAACTGGAAAAATCGAGTACAACCCATAACACATAGACATTCTGAGAAAAAAGTAACTGTATATTTAGCTGGTCCTTTTTTTACTCTTTCACAGATATGGCTTATTAATGAAACTAGAAGGTTATTGAGTGAGATGGGACTGATAGTATTTTCACCATATCATGAAATTTGAACTGGAGATGCTATAATGGTCGTACCTAAAGATATAGATGGAATAAATAATTCAGATATTATATTTGCCATCTTTGATGGAAAAGATTCTGGGACTATTTTTGAAATAGGATACGCTACAGCAAAGAATAAACCTGTTATTGTCTATAATGAAAATGAAAAATCAGAAGACTTAAAGATGATGGAAGGGACAGATTGTATAGTTATTAAAGACTATGTAAGTGCTATTTACCGCACCCTATGGG  >  1/295827‑296289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑