Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,977,775	T→C	V372V (GTA→GTG)	unknown ←	RTX toxin transporter, ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,977,775	0	T	C	100.0%	227.4 / NA	75	V372V (GTA→GTG)	unknown	RTX toxin transporter, ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/42);  total (33/42)

CTCTGTAGGCGAATTAAGCACATCACCCAAACGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACAGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGGTTGATCACCATAACGGCTTTCTGGATCAATTGGATACCTTGCTGTCCGATCGTTGCAAGTACAGTCACTTTAAAACTCACAGCAACATAACTCGCTAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAAT  >  1/1977532‑1978009                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           ctctGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCa                                                                                                                                                                                                                                     >  1:150534/1‑251 (MQ=255)  tctGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCAc                                                                                                                                                                                                                                    <  1:741488/251‑1 (MQ=255)   ctGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCa                                                                                                                                                                                                                                     >  1:375073/1‑249 (MQ=255)          cGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAAc                                                                                                                                                                                                                            <  1:475574/251‑1 (MQ=255)           gAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTaaaa                                                                                                                                                                                                                             >  1:197262/1‑249 (MQ=255)                aaGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAg                                                                                                                                                                                                                      >  1:405483/1‑251 (MQ=255)                     caTCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACa                                                                                                                                                                                                                 >  1:668156/1‑251 (MQ=255)                      aTCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACAt                                                                                                                                                                                                                <  1:7766/251‑1 (MQ=255)                        cacctaagcGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATaa                                                                                                                                                                                                              >  1:546045/9‑251 (MQ=255)                         acctaagcGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATaa                                                                                                                                                                                                              >  1:120985/8‑250 (MQ=255)                               gcGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGcca                                                                                                                                                                                                       >  1:14781/2‑249 (MQ=255)                                      aCAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAAc                                                                                                                                                                                                                            >  1:490144/1‑223 (MQ=255)                                       cAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTa                                                                                                                                                                                               >  1:758095/1‑251 (MQ=255)                                           aaTACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATccc                                                                                                                                                                                           <  1:762244/251‑1 (MQ=255)                                            aTACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTTCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATcc                                                                                                                                                                                            <  1:158672/249‑1 (MQ=255)                                                      gTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATcc                                                                                                                                                                                            >  1:855756/1‑239 (MQ=255)                                                        tGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCAt                                                                                                                                                                              >  1:320705/1‑251 (MQ=255)                                                                           gggCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACAt                                                                                                                                                                                                                <  1:895655/198‑1 (MQ=255)                                                                           gggCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCAt                                                                                                                                                            >  1:88712/1‑250 (MQ=255)                                                                                   cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                                   <  1:42189/251‑1 (MQ=255)                                                                                   cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                                   <  1:140455/251‑1 (MQ=255)                                                                                   cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                                   <  1:510748/251‑1 (MQ=255)                                                                                   cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                                   <  1:790101/251‑1 (MQ=255)                                                                                     aaTCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTg                                                                                                                                                 <  1:804851/251‑1 (MQ=255)                                                                                            gggCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAAt                                                                                                                                          >  1:631975/1‑251 (MQ=255)                                                                                               cTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGc                                                                                                                                       <  1:830417/251‑1 (MQ=255)                                                                                                     atTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCAc                                                                                                                                 <  1:319263/251‑1 (MQ=255)                                                                                                                 aGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACttt                                                                                                                                                                                                                                 >  1:281240/1‑143 (MQ=255)                                                                                                                     tatTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTgagg                                                                                                                                                                        >  1:40327/1‑196 (MQ=255)                                                                                                                           aTGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGcc                                                                                                                                                              <  1:8626/200‑1 (MQ=255)                                                                                                                            tGCAATTAGCTGACCAATGCTTGAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGc                                                                                                                                                               <  1:485130/198‑1 (MQ=255)                                                                                                                             gCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGAtt                                                                                                         >  1:356995/1‑251 (MQ=255)                                                                                                                                     cTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGc                                                                                                   <  1:842801/249‑1 (MQ=255)                                                                                                                                       gACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAAc                                                                                                                                                                                                             >  1:388129/1‑141 (MQ=255)                                                                                                                                         ccAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                                                                   <  1:160750/197‑1 (MQ=255)                                                                                                                                            aTACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTg                                                                                           >  1:514761/1‑250 (MQ=255)                                                                                                                                              aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGAc                                                                                                              <  1:172235/229‑1 (MQ=255)                                                                                                                                              aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGa                                                                                        >  1:188431/1‑251 (MQ=255)                                                                                                                                              aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGa                                                                                        >  1:569366/1‑251 (MQ=255)                                                                                                                                              aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGa                                                                                        >  1:744846/1‑251 (MQ=255)                                                                                                                                                            aaaTAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGc                                                                          <  1:18592/251‑1 (MQ=255)                                                                                                                                                               tAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGt                                                                       >  1:148509/1‑251 (MQ=255)                                                                                                                                                                aaCTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTGAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGt                                                                       >  1:184848/1‑250 (MQ=255)                                                                                                                                                                   tAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTcc                                                                   <  1:629131/251‑1 (MQ=255)                                                                                                                                                                           cgcCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                            <  1:50691/250‑1 (MQ=255)                                                                                                                                                                           cgcCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                            <  1:563140/250‑1 (MQ=255)                                                                                                                                                                           cgcCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                            <  1:6594/250‑1 (MQ=255)                                                                                                                                                                               aaGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGAc                                                       <  1:494647/251‑1 (MQ=255)                                                                                                                                                                               aaGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGAc                                                       <  1:759560/251‑1 (MQ=255)                                                                                                                                                                               aaGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGAc                                                       <  1:899703/251‑1 (MQ=255)                                                                                                                                                                                 gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGc                                                                                                                                                                                                                     <  1:552145/91‑1 (MQ=255)                                                                                                                                                                                 gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGcc                                                                                                                                                              <  1:897809/146‑1 (MQ=255)                                                                                                                                                                                 gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTatcatc                                                                              <  1:395511/226‑1 (MQ=255)                                                                                                                                                                                 gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                            <  1:160408/244‑1 (MQ=255)                                                                                                                                                                                 gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACta                                                     >  1:485025/1‑251 (MQ=255)                                                                                                                                                                                      agattGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTcaacaa                                                                                                                      >  1:405157/4‑181 (MQ=255)                                                                                                                                                                                         ttGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                             <  1:351494/251‑1 (MQ=255)                                                                                                                                                                                              cACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                             <  1:95563/246‑1 (MQ=255)                                                                                                                                                                                              cACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGAc                                         >  1:134940/1‑250 (MQ=255)                                                                                                                                                                                                ccATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                            >  1:495826/1‑229 (MQ=255)                                                                                                                                                                                                ccATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACtaata                                                  >  1:816399/1‑239 (MQ=255)                                                                                                                                                                                                ccATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                             <  1:451660/244‑1 (MQ=255)                                                                                                                                                                                                ccATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCaa                                      >  1:343116/1‑251 (MQ=255)                                                                                                                                                                                                       ggCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAAc                                             >  1:268936/1‑237 (MQ=255)                                                                                                                                                                                                          tttCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAAc                            <  1:325811/251‑1 (MQ=255)                                                                                                                                                                                                                     gttGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACtaata                                                  <  1:195721/217‑1 (MQ=255)                                                                                                                                                                                                                      ttGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAAc                <  1:385120/251‑1 (MQ=255)                                                                                                                                                                                                                      ttGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTAATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAAc                <  1:17494/251‑1 (MQ=255)                                                                                                                                                                                                                             ccTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACaa                          <  1:25969/234‑1 (MQ=255)                                                                                                                                                                                                                             ccTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAAc                <  1:711694/244‑1 (MQ=255)                                                                                                                                                                                                                             ccTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTAc         <  1:368690/251‑1 (MQ=255)                                                                                                                                                                                                                                tgctgcCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAAc      <  1:322348/251‑1 (MQ=255)                                                                                                                                                                                                                                    gcccGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt                                                            >  1:234815/3‑193 (MQ=255)                                                                                                                                                                                                                                    gcccGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAAt  >  1:783756/3‑251 (MQ=255)                                                                                                                                                                                                                                             ttGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAAc      <  1:305816/238‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           CTCTGTAGGCGAATTAAGCACATCACCCAAACGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACAGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGGTTGATCACCATAACGGCTTTCTGGATCAATTGGATACCTTGCTGTCCGATCGTTGCAAGTACAGTCACTTTAAAACTCACAGCAACATAACTCGCTAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTAATAAAAACAGACCAAATGACATAACAAGGTAAAGAACCGAGTACAACTAAT  >  1/1977532‑1978009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑