Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,977,715	G→A	N392N (AAC→AAT)	unknown ←	RTX toxin transporter, ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,977,715	0	G	A	100.0%	208.4 / NA	73	N392N (AAC→AAT)	unknown	RTX toxin transporter, ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/39);  total (34/39)

ACTTAATATTTCTAAAACTAATCTCACCCTGGATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCCAAACGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACAGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGGTTGATCACCATAACGGCTTTCTGGATCAATTGGATACCTTGCTGTCCGATCGTTGCAAGTACAGTCACTTTAAAACTCACAGCAACATAACTCGCTAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTA  >  1/1977468‑1977958                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    aCTTAATATTTCTAAAACTAATCTCACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTg                                                                                                                                                                                                                                                  <  1:423497/251‑1 (MQ=255)                        tcACCCTGAATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGAt                                                                                                                                                                                                                           <  1:802112/251‑1 (MQ=255)                                   ttCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATAc                                                                                                                                                                                                                <  1:380504/251‑1 (MQ=255)                                         tAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCAt                                                                                                                                                                                                                                          <  1:127113/219‑1 (MQ=255)                                                         gTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGaaac                                                                                                                                                                                                                >  1:629990/1‑226 (MQ=255)                                                         gTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATAc                                                                                                                                                                                                                >  1:297322/1‑229 (MQ=255)                                                         gTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATAc                                                                                                                                                                                                                <  1:664347/229‑1 (MQ=255)                                                         gTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAg                                                                                                                                                                                          >  1:111704/1‑251 (MQ=255)                                                         gTATTATTCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAg                                                                                                                                                                                          >  1:544168/1‑251 (MQ=255)                                                           attattCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAgca                                                                                                                                                                                        <  1:801320/251‑3 (MQ=255)                                                              attCTCTGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGAtaagttg                                                                                                                                                                                                                     >  1:838608/1‑213 (MQ=255)                                                                 ctctGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCa                                                                                                                                                                                  >  1:150534/1‑251 (MQ=255)                                                                  tctGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCAc                                                                                                                                                                                 <  1:741488/251‑1 (MQ=255)                                                                   ctGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCtt                                                                                                                                                                                                                                  >  1:287259/1‑201 (MQ=255)                                                                   ctGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATAcc                                                                                                                                                                                                               >  1:96953/1‑220 (MQ=255)                                                                   ctGTAGGCGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCa                                                                                                                                                                                  >  1:375073/1‑249 (MQ=255)                                                                          cGAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAAc                                                                                                                                                                         <  1:475574/251‑1 (MQ=255)                                                                           gAATTAAGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTaaaa                                                                                                                                                                          >  1:197262/1‑249 (MQ=255)                                                                                aaGCACATCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAg                                                                                                                                                                   >  1:405483/1‑251 (MQ=255)                                                                                     caTCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACa                                                                                                                                                              >  1:668156/1‑251 (MQ=255)                                                                                      aTCACCTAAGCGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACAt                                                                                                                                                             <  1:7766/251‑1 (MQ=255)                                                                                        cacctaagcGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATaa                                                                                                                                                           >  1:546045/9‑251 (MQ=255)                                                                                         acctaagcGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGAt                                                                                                                                                                                                                  <  1:190621/188‑1 (MQ=255)                                                                                         acctaagcGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATAc                                                                                                                                                                                                                >  1:645826/8‑197 (MQ=255)                                                                                         acctaagcGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAgca                                                                                                                                                                                        <  1:168122/214‑3 (MQ=255)                                                                                         acctaagcGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATaa                                                                                                                                                           >  1:120985/8‑250 (MQ=255)                                                                                               gcGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGcca                                                                                                                                                    >  1:14781/2‑249 (MQ=255)                                                                                                      aCAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAAc                                                                                                                                                                         >  1:490144/1‑223 (MQ=255)                                                                                                       cAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTa                                                                                                                                            >  1:758095/1‑251 (MQ=255)                                                                                                           aaTACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATccc                                                                                                                                        <  1:762244/251‑1 (MQ=255)                                                                                                            aTACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTgcc                                                                                                                                                                                                       >  1:868486/1‑185 (MQ=255)                                                                                                            aTACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTTCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATcc                                                                                                                                         <  1:158672/249‑1 (MQ=255)                                                                                                                      gTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATcc                                                                                                                                         >  1:855756/1‑239 (MQ=255)                                                                                                                        tGGAAATCTTGCCAGATCTGGGCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCAt                                                                                                                           >  1:320705/1‑251 (MQ=255)                                                                                                                                           gggCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACAt                                                                                                                                                             <  1:895655/198‑1 (MQ=255)                                                                                                                                           gggCTAAACGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCAt                                                                                                         >  1:88712/1‑250 (MQ=255)                                                                                                                                                   cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                <  1:790101/251‑1 (MQ=255)                                                                                                                                                   cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                <  1:42189/251‑1 (MQ=255)                                                                                                                                                   cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                <  1:510748/251‑1 (MQ=255)                                                                                                                                                   cGAATCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                <  1:140455/251‑1 (MQ=255)                                                                                                                                                     aaTCACGGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTg                                                                                              <  1:804851/251‑1 (MQ=255)                                                                                                                                                            gggCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAAt                                                                                       >  1:631975/1‑251 (MQ=255)                                                                                                                                                               cTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGc                                                                                    <  1:830417/251‑1 (MQ=255)                                                                                                                                                                     atTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCAc                                                                              <  1:319263/251‑1 (MQ=255)                                                                                                                                                                                 aGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACttt                                                                                                                                                                              >  1:281240/1‑143 (MQ=255)                                                                                                                                                                                     tatTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTgagg                                                                                                                     >  1:40327/1‑196 (MQ=255)                                                                                                                                                                                           aTGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGcc                                                                                                           <  1:8626/200‑1 (MQ=255)                                                                                                                                                                                            tGCAATTAGCTGACCAATGCTTGAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGc                                                                                                            <  1:485130/198‑1 (MQ=255)                                                                                                                                                                                             gCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAgca                                                                                                                                                                                        <  1:306740/121‑3 (MQ=255)                                                                                                                                                                                             gCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGAtt                                                      >  1:356995/1‑251 (MQ=255)                                                                                                                                                                                                     cTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGc                                                <  1:842801/249‑1 (MQ=255)                                                                                                                                                                                                       gACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAAc                                                                                                                                                          >  1:388129/1‑141 (MQ=255)                                                                                                                                                                                                         ccAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAAt                                                                                                <  1:160750/197‑1 (MQ=255)                                                                                                                                                                                                            aTACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTg                                        >  1:514761/1‑250 (MQ=255)                                                                                                                                                                                                              aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGAc                                                           <  1:172235/229‑1 (MQ=255)                                                                                                                                                                                                              aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGa                                     >  1:569366/1‑251 (MQ=255)                                                                                                                                                                                                              aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGa                                     >  1:744846/1‑251 (MQ=255)                                                                                                                                                                                                              aCTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGa                                     >  1:188431/1‑251 (MQ=255)                                                                                                                                                                                                                            aaaTAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGc                       <  1:18592/251‑1 (MQ=255)                                                                                                                                                                                                                               tAACTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGt                    >  1:148509/1‑251 (MQ=255)                                                                                                                                                                                                                                aaCTAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTGAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGt                    >  1:184848/1‑250 (MQ=255)                                                                                                                                                                                                                                   tAAATGTGCGCCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTcc                <  1:629131/251‑1 (MQ=255)                                                                                                                                                                                                                                           cgcCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt         <  1:6594/250‑1 (MQ=255)                                                                                                                                                                                                                                           cgcCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt         <  1:563140/250‑1 (MQ=255)                                                                                                                                                                                                                                           cgcCAAGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt         <  1:50691/250‑1 (MQ=255)                                                                                                                                                                                                                                               aaGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGAc    <  1:759560/251‑1 (MQ=255)                                                                                                                                                                                                                                               aaGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGAc    <  1:494647/251‑1 (MQ=255)                                                                                                                                                                                                                                               aaGCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGAc    <  1:899703/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGc                                                                                                                                                                  <  1:552145/91‑1 (MQ=255)                                                                                                                                                                                                                                                 gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGcc                                                                                                           <  1:897809/146‑1 (MQ=255)                                                                                                                                                                                                                                                 gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTatcatc                           <  1:395511/226‑1 (MQ=255)                                                                                                                                                                                                                                                 gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAAt         <  1:160408/244‑1 (MQ=255)                                                                                                                                                                                                                                                 gCCATAGATTGATCACCATAACGGCTTTCTGGATAAGTTGGATACCTTGCTGCCCGATCGTTGCAAGCACGGTCACTTTAAAACTCGCAGCAACATAACTCGCCAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACta  >  1:485025/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    ACTTAATATTTCTAAAACTAATCTCACCCTGGATTTCCGGTAATGATACTGAGGCGGTATTATTCTCTGTAGGCGAATTAAGCACATCACCCAAACGAGTAACAGAAATACCGACCTGTTGGAAATCTTGCCAGATCTGGGCTAAACGAATCACAGGGCTAATAATTTGTCCCGCAAGCATATTAAATGCAATTAGCTGACCAATACTTAAATCACCGGAAATAACTAAATGTGCGCCAAGCCATAGGTTGATCACCATAACGGCTTTCTGGATCAATTGGATACCTTGCTGTCCGATCGTTGCAAGTACAGTCACTTTAAAACTCACAGCAACATAACTCGCTAACTGTTTATCCCAAATGTTAGTCATTTGAGGAGAAATAGCCATCGCTTTAATTGTATTAATGGCAGTCACTGATTCAACAAGAAAAGACTGATTGTCGGCATTTCTTGCGAACTTATCATCAAGCCGTCTCCTAAGAATAGGACTA  >  1/1977468‑1977958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑