Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,525	G→A	V51V (GTG→GTA)	unknown →	DNA polymerase III beta subunit (EC 2.7.7.7)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,525	0	G	A	100.0%	214.8 / NA	74	V51V (GTG→GTA)	unknown	DNA polymerase III beta subunit (EC 2.7.7.7)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/30);  total (44/30)

TGCCGCACCATTGCAGGATTCCGCGACACCGATCCGAATATTCAAGAAGATTGGGCTAACTTAATCCGAATTTTATCCGTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTGGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAATCGGAAGTGGATTTTACGCTTGAGCAAGCC  >  1/1279‑1771                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       tGCCGCACCATTGCAGGATTCCGCGACACCGATCCGAATATTCAAGAAGATTGGGCTAACTTAATCCGAATTTTATCCGTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAAc                                                                                                                                                                                                                                                    <  1:749509/251‑1 (MQ=255)           ttGCAGGATTCCGCGACACCGATCCGAATATTCAAGAAGATTGGGCTAACTTAATCCGAATTTTATCCGTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCa                                                                                                                                                                                                                                          >  1:650667/1‑251 (MQ=255)                     ccGCGACACCGATCCGAATATTCAAGAAGATTGGGCTAACTTAATCCGAATTTTATCCGTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTg                                                                                                                                                                                                                                                  >  1:33056/1‑233 (MQ=255)                             ccGATCCGAATATTCAAGAAGATTGGGCTAACTTAATCCGAATTTTATCCGTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCAccc                                                                                                                                                                                                                                           <  1:821528/232‑1 (MQ=255)                                         ttCAAGAAGATTGGGCTAACTTAATCCGAATTTTATCCGTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTcc                                                                                                                                                                                                                                               >  1:461317/1‑216 (MQ=255)                                             agaagaTTGGGCTAACTTAATCCGAATTTTATCCGTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAAccc                                                                                                                                                                                                                                     <  1:156525/222‑1 (MQ=255)                                                      ggCTAACTTAATCCGAATTTTATCCGTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTAt                                                                                                                                                                                                <  1:732422/250‑1 (MQ=255)                                                          aaCTCAATCCGAATTTTATCCGTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCgg                                                                                                                                                                                           >  1:647135/1‑251 (MQ=255)                                                                     aaTTTTATCCGTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTAc                                                                                                                                                                                                                               >  1:301993/1‑204 (MQ=255)                                                                               gTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTcc                                                                                                                                                                                                                                               >  1:92293/1‑178 (MQ=255)                                                                               gTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTAc                                                                                                                                                                                                                               <  1:826456/194‑1 (MQ=255)                                                                               gTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGc                                                                                                                                                                                                                        >  1:143181/1‑201 (MQ=255)                                                                               gTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGc                                                                                                                                                                                                                        >  1:191977/1‑201 (MQ=255)                                                                               gTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTcc                                                                                                                                                                                             <  1:41949/228‑1 (MQ=255)                                                                               gTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATAttt                                                                                                                                                                      >  1:120343/1‑251 (MQ=255)                                                                               gTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATAttt                                                                                                                                                                      >  1:352121/1‑251 (MQ=255)                                                                                    ggAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGAc                                                                                                                                                                                                              >  1:178908/1‑206 (MQ=255)                                                                                                tgCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGAt                                                                                                                                                     <  1:174193/251‑1 (MQ=255)                                                                                                        attattTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGa                                                                                                                                             >  1:444237/1‑251 (MQ=255)                                                                                                                         tttACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTg                                                                                                                            >  1:209074/1‑251 (MQ=255)                                                                                                                           tACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGAc                                                                                                                                                                                                              >  1:327409/1‑167 (MQ=255)                                                                                                                                 aaCCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTgaag                                                                                                                         >  1:790273/1‑246 (MQ=255)                                                                                                                                   cccTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAAccc                                                                                                                                                                                                                                     >  1:643248/1‑136 (MQ=255)                                                                                                                                   cccTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGat                                                                                                                                                                          <  1:778016/195‑1 (MQ=255)                                                                                                                                   cccTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGaaa                                                                                                                                           >  1:791803/1‑226 (MQ=255)                                                                                                                                   cccTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATcg                                                                                                                  >  1:325286/1‑251 (MQ=255)                                                                                                                                    ccTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGc                                                                                                                                                >  1:121972/1‑220 (MQ=255)                                                                                                                                     cTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATcgcg                                                                                                                >  1:606115/1‑251 (MQ=255)                                                                                                                                       tACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGc                                                                                                                                                >  1:732998/1‑217 (MQ=255)                                                                                                                                       tACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCt                                                                                                              <  1:605013/251‑1 (MQ=255)                                                                                                                                         caacaaGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTAt                                                                                                            >  1:867580/1‑251 (MQ=255)                                                                                                                                            caaGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATtgt                                                                                                         <  1:250532/251‑1 (MQ=255)                                                                                                                                                tgtgCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGc                                                                                                                                                <  1:138109/208‑1 (MQ=255)                                                                                                                                                  tgCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTc                                                                                                   <  1:88362/251‑1 (MQ=255)                                                                                                                                                   gcggcgTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTc                                                                                                   >  1:592381/1‑250 (MQ=255)                                                                                                                                                             gAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTa                                                                                        >  1:6901/1‑251 (MQ=255)                                                                                                                                                                   ccgccCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAAtt                                                                                    <  1:238319/249‑1 (MQ=255)                                                                                                                                                                         gAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTc                                                                                   <  1:452071/244‑1 (MQ=255)                                                                                                                                                                           atatTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCAcc                                                                                                                                                                                                                                            >  1:254563/1‑89 (MQ=255)                                                                                                                                                                                   gtgtTAAATAATGTATTGCTGCAAATTGAAAATACTCAATTAAATATTACCGGGACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGc                                                                   >  1:650600/1‑250 (MQ=255)                                                                                                                                                                                        aaaTAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCcggcgg                                                             >  1:567031/1‑251 (MQ=255)                                                                                                                                                                                        aaaTAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCcggcgg                                                             >  1:642863/1‑251 (MQ=255)                                                                                                                                                                                          ataatGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAAc                                                                                                                                                                                                                                                    <  1:16107/66‑1 (MQ=255)                                                                                                                                                                                          ataatGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATaa                                                                                                                                                   >  1:807645/1‑163 (MQ=255)                                                                                                                                                                                          ataatGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGAt                                                                                                                    <  1:831890/194‑1 (MQ=255)                                                                                                                                                                                          ataatGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATtg                                                                                                          >  1:741337/1‑204 (MQ=255)                                                                                                                                                                                          ataatGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAAtt                                                                                    >  1:105552/1‑226 (MQ=255)                                                                                                                                                                                               gTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTa                                                      >  1:123075/1‑251 (MQ=255)                                                                                                                                                                                                                       cAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGAt                                                                                                                                                     <  1:780683/132‑1 (MQ=255)                                                                                                                                                                                                                       cAATTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAAt                               >  1:146275/1‑250 (MQ=255)                                                                                                                                                                                                                         aTTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTc                                                                                                                                                                                              <  1:506136/89‑1 (MQ=255)                                                                                                                                                                                                                         aTTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGc                                                                                                                                                <  1:778412/135‑1 (MQ=255)                                                                                                                                                                                                                         aTTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGAt                                                                                                                    <  1:245332/163‑1 (MQ=255)                                                                                                                                                                                                                         aTTAAATATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGAt                                                        <  1:309184/223‑1 (MQ=255)                                                                                                                                                                                                                             aaTATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCAc                                                                        <  1:376216/203‑1 (MQ=255)                                                                                                                                                                                                                             aaTATTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAATCGGAAGt                        >  1:97192/1‑251 (MQ=255)                                                                                                                                                                                                                              atatTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGAt                                                                                                                                                     >  1:409639/1‑125 (MQ=255)                                                                                                                                                                                                                               tatTACCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAATCgg                            <  1:467148/245‑1 (MQ=255)                                                                                                                                                                                                                                   aCCGGTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAATCGGAAGTGGAttt                  <  1:241532/251‑1 (MQ=255)                                                                                                                                                                                                                                       gTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGAt                                                        >  1:113477/1‑209 (MQ=255)                                                                                                                                                                                                                                       gTACGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAATCGGAAGTGGATTTTACg              >  1:311256/1‑251 (MQ=255)                                                                                                                                                                                                                                         aCGGATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAATCGGAAGTGGATTTTACGCt            <  1:793028/251‑1 (MQ=255)                                                                                                                                                                                                                                           ggATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTcc                                                                                                                                                                                             <  1:138150/72‑1 (MQ=255)                                                                                                                                                                                                                                            gATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAATCGGAAGTGGAt                    <  1:387371/240‑1 (MQ=255)                                                                                                                                                                                                                                            gATTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAATCGGAAGTGGATTTTACGCTTGa         >  1:693637/1‑251 (MQ=255)                                                                                                                                                                                                                                             aTTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAATCGGAAGTGGATTTTACGCTTGAg        <  1:83223/251‑1 (MQ=255)                                                                                                                                                                                                                                             aTTTAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAATCGGAAGTGGATTTTACGCTTGAg        >  1:517142/1‑251 (MQ=255)                                                                                                                                                                                                                                                tAGAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAAc                                                                                                                                                                                                                   <  1:720409/45‑1 (MQ=255)                                                                                                                                                                                                                                                  gAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAATCGGAAGTGGATTTTACGCTTGAGCAAg    >  1:258171/1‑250 (MQ=255)                                                                                                                                                                                                                                                  gAAGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAATCGGAAGTGGATTTTACGCTTGAGCAAg    >  1:40055/1‑250 (MQ=255)                                                                                                                                                                                                                                                   aaGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGAt                                                        >  1:809819/1‑197 (MQ=255)                                                                                                                                                                                                                                                   aaGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAATCGGAAGTGGATTTTACg              >  1:153706/1‑239 (MQ=255)                                                                                                                                                                                                                                                   aaGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAATCGGAAGTGGATTTTACGCTTGAGCAAGcc  <  1:350123/251‑1 (MQ=255)                                                                                                                                                                                                                                                   aaGTAGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAATCGGAAGTGGATTTTACGCTTGAGCAAGc   >  1:865631/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       TGCCGCACCATTGCAGGATTCCGCGACACCGATCCGAATATTCAAGAAGATTGGGCTAACTTAATCCGAATTTTATCCGTATAGGAGTCATATGATGCAATTTATTATTTCCAGAGAAAATTTACTAAAACCCTTACAACAAGTGTGCGGCGTGCTGAATAGCCGCCCGAATATTCCGGTGTTAAATAATGTATTGCTGCAAATTGAAAATAATCAATTAAATATTACCGGTACGGATTTAGAAGTGGAACTGTCCACCCAAACCCCATTACTTCATGCGGAACAAGACGGCAAATTCACTATTCCGGCGAAAAAATTCCTGGATATTTGTCGCAGCCTGCCGGATAATGCAGAAATTGCTGTGACATTTGAAGAAGATCGCGCTATTGTGCGTTCCGATCGCAGTAAATTCAATTTATCCACTTTGCCGGCGGAAGATTATCCGAATTTAACGGACTGGCAATCGGAAGTGGATTTTACGCTTGAGCAAGCC  >  1/1279‑1771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑