Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,977,142	T→G	G583G (GGA→GGC)	unknown ←	RTX toxin transporter, ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,977,142	0	T	G	100.0%	169.0 / NA	60	G583G (GGA→GGC)	unknown	RTX toxin transporter, ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (35/25);  total (35/25)

GCACAGTACGATTTTGGCAAATTTTATGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCTTGGGTTATTGACTAGTGCCCTTGCTATCGCGATCCGTTGACGTTGTCCTCCGGACAAACCGGCTCCCTGTT‑CCCCCACAACCGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCTCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGGCATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAAGACGACACCGACTTGCCGGCGCAACCAGTTTGGATCGGCTAGCGCCAAATCGTGACCGTCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATGAGTTTAGTAAGCGTACTTTTCCCCGAACCTGAAC  >  1/1976919‑1977383                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  gCACGGTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAAc                                                                                                                                                                                                                         >  1:30959/1‑250 (MQ=255)      gTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCtttt                                                                                                                                                                                                                    >  1:802621/1‑250 (MQ=255)      gTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCtttt                                                                                                                                                                                                                    >  1:751666/1‑250 (MQ=255)      gTACGATCTTGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCtttt                                                                                                                                                                                                                    >  1:306366/1‑250 (MQ=255)              ttGGCAAATTTTGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTgaca                                                                                                                                                                                                           <  1:468003/251‑4 (MQ=255)                      ttttGTGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCTTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGa                                                                                                                                                                                                   >  1:799576/1‑251 (MQ=255)                       tttgtgcatattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGAt                                                                                                                                                                                                  <  1:348178/247‑1 (MQ=255)                             catattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGtt                                                                                                                                                                                             <  1:661249/250‑1 (MQ=255)                              atattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGAt                                                                                                                                                                                                  >  1:569305/1‑244 (MQ=255)                              atattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTaa                                                                                                                                                                                           >  1:172610/1‑251 (MQ=255)                               tattatgcataATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTaa                                                                                                                                                                                           >  1:813030/1‑250 (MQ=255)                                        taATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATtc                                                                                                                                                                                 <  1:833537/251‑1 (MQ=255)                                                ttttCAGATTCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGAt                                                                                                                                                                         <  1:705208/251‑1 (MQ=255)                                                 tttCAGATTCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATa                                                                                                                                                                        <  1:885928/251‑1 (MQ=255)                                                         tCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGAtt                                                                                                                                                                <  1:563836/251‑1 (MQ=255)                                                           gTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAAcc                                                                                                                                                                                                                        <  1:81868/193‑1 (MQ=255)                                                           gTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTctctct                                                                                                                                                                            <  1:661709/237‑1 (MQ=255)                                                                  aGTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAaca                                                                                                                                                        >  1:360603/1‑250 (MQ=255)                                                                   gTGCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCgg                                                                                                                                                                                                    >  1:72665/1‑205 (MQ=255)                                                                     gCACTGGTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACAtt                                                                                                                                                    >  1:677833/1‑251 (MQ=255)                                                                          ggTTGCTTCATCAAAAATCAAAATCCGTGGGTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTg                                                                                                                                                                                                <  1:768141/202‑1 (MQ=255)                                                                                                       ggTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCgg                                                                                                                                                                                                    <  1:684436/169‑1 (MQ=255)                                                                                                       ggTTATTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGgcgtaa                                                                                                                   >  1:496902/1‑247 (MQ=255)                                                                                                           aTTGACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATtc                                                                                                                                                                                 <  1:790001/184‑1 (MQ=255)                                                                                                              gACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATtc                                                                                                                                                                                 <  1:11855/181‑1 (MQ=255)                                                                                                              gACTAATGCCCTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGttt                                                                                                            >  1:592006/1‑250 (MQ=255)                                                                                                                      cccTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCgg                                                                                                     >  1:309460/1‑249 (MQ=255)                                                                                                                      cccTTGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCt                                                                                                   <  1:267016/251‑1 (MQ=255)                                                                                                                          tGCTATCGCGATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTTCCCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAgt                                                                                                >  1:58778/1‑250 (MQ=255)                                                                                                                                cgcgATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAAt                                                                                         >  1:46291/1‑251 (MQ=255)                                                                                                                                  cgATCCGTTGGCGTTGTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCg                                                                                       <  1:310193/251‑1 (MQ=255)                                                                                                                                                 gTCCTCCGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAg                                                                        >  1:679483/1‑251 (MQ=255)                                                                                                                                                   cctccGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGa                                                                       >  1:548547/1‑250 (MQ=255)                                                                                                                                                   cctccGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGa                                                                       >  1:480375/1‑250 (MQ=255)                                                                                                                                                   cctccGGACAGACCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGAc                                                                      <  1:234371/251‑1 (MQ=255)                                                                                                                                                             gaCCGGCTCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGAc                                                                      >  1:375659/2‑241 (MQ=255)                                                                                                                                                                    tCCCTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGaa                                                     <  1:835748/251‑1 (MQ=255)                                                                                                                                                                     cccTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACAcctac                                                                                                                               <  1:371860/176‑4 (MQ=255)                                                                                                                                                                       cTGTT‑CCCCCACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAatat                                                  <  1:712496/251‑1 (MQ=255)                                                                                                                                                                             cccccACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGgacg                                                                                                                                       >  1:554592/1‑161 (MQ=255)                                                                                                                                                                             cccccACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATaaaaa                                             <  1:654123/251‑1 (MQ=255)                                                                                                                                                                              ccccACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAatat                                                                                                                                                                                   >  1:746974/1‑116 (MQ=255)                                                                                                                                                                              ccccACAACAGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCag                                                                                                                                           >  1:56439/1‑155 (MQ=255)                                                                                                                                                                                     acagtgtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGAt                                     >  1:115730/4‑251 (MQ=255)                                                                                                                                                                                     acagtgtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGAt                                     >  1:61340/4‑251 (MQ=255)                                                                                                                                                                                        gtgtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGAc                                                                      >  1:99340/1‑215 (MQ=255)                                                                                                                                                                                        gtgtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGAc                                                                      >  1:140104/1‑215 (MQ=255)                                                                                                                                                                                        gtgtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTc                                                          >  1:884549/1‑227 (MQ=255)                                                                                                                                                                                         tgtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGt                                 >  1:317725/1‑251 (MQ=255)                                                                                                                                                                                          gtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCTGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGCATATAAAAACGTTGGATAAGtt                                >  1:218832/1‑251 (MQ=255)                                                                                                                                                                                          gtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATtctctc                                                                                                                                                                             <  1:160866/110‑1 (MQ=255)                                                                                                                                                                                          gtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCt                                                                                                                                                >  1:81250/1‑139 (MQ=255)                                                                                                                                                                                          gtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGAc                                                                      <  1:226283/213‑1 (MQ=255)                                                                                                                                                                                          gtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGcccc                                                               <  1:711476/220‑1 (MQ=255)                                                                                                                                                                                          gtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGccc                                                                >  1:868236/1‑219 (MQ=255)                                                                                                                                                                                          gtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGcc                                                                 <  1:358141/218‑1 (MQ=255)                                                                                                                                                                                          gtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGtt                                >  1:264155/1‑251 (MQ=255)                                                                                                                                                                                          gtTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCGCCCACAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGtt                                >  1:163117/1‑251 (MQ=255)                                                                                                                                                                                                    tctcTTAATTCAGAAATAAAATCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGt                      >  1:297873/1‑251 (MQ=255)                                                                                                                                                                                                                        aTCGTGCGCGCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGACATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAGGACGACACCTACTTGCCGGCGTAACCAGTTTGGATCGGCTAGTGCCAAATCGTGACCATCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATAAGTTTAGTAAGCGTACTTTTTCCCGAACCTGAAc  <  1:764428/251‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  GCACAGTACGATTTTGGCAAATTTTATGCATATTATGCATAATGATATTTTCAGATTCGTAATCAAGTGCACTGGTTGCTTCATCAAAAATCAAAATCCTTGGGTTATTGACTAGTGCCCTTGCTATCGCGATCCGTTGACGTTGTCCTCCGGACAAACCGGCTCCCTGTT‑CCCCCACAACCGTGTTATAACCTTCTCTTAATTCAGAAATAAAATCGTGCGCTCCCGCAAGTTTTGCCGCGGCAATAACCTTTTCCATTGGCATTCCCGGATTGGTTAACGCAATATTCTCTCTGATACTTCGATTAAGCAACACATTATCTTGCAAGACGACACCGACTTGCCGGCGCAACCAGTTTGGATCGGCTAGCGCCAAATCGTGACCGTCAATTAAGACTTGCCCCTGCTCCGGAATATAAAAACGTTGGATGAGTTTAGTAAGCGTACTTTTCCCCGAACCTGAAC  >  1/1976919‑1977383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑