Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,976,498	C→G	T85T (ACG→ACC)	unknown ←	RTX toxin transporter, determinant D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,976,498	0	C	G	100.0%	193.5 / NA	67	T85T (ACG→ACC)	unknown	RTX toxin transporter, determinant D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (33/34);  total (33/34)

TTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCCAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATGTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCAAATTTTCCTGAAGCCGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTTTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAACCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAA  >  1/1976267‑1976731                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          tttttCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACAt                                                                                                                                                                                                                        <  1:130966/251‑1 (MQ=255) tttttCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACAt                                                                                                                                                                                                                        <  1:689656/251‑1 (MQ=255)      cAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTa                                                                                                                                                                                                                   >  1:575699/1‑251 (MQ=255)            cTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAg                                                                                                                                                                                                                <  1:811165/248‑1 (MQ=255)            cTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAg                                                                                                                                                                                                                <  1:625081/248‑1 (MQ=255)            cTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAg                                                                                                                                                                                                                <  1:5637/248‑1 (MQ=255)            cTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGAt                                                                                                                                                                                                              <  1:377746/250‑1 (MQ=255)             ttCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAg                                                                                                                                                                                                            <  1:211954/251‑1 (MQ=255)               ccAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGaa                                                                                                                                                                                                          >  1:505605/1‑251 (MQ=255)                cAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCcaca                                                                                                                                                                                                                         <  1:172566/235‑1 (MQ=255)                cAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGaaa                                                                                                                                                                                                         <  1:353509/251‑1 (MQ=255)                 aaTAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAAc                                                                                                                                                                                                        <  1:784455/251‑1 (MQ=255)                 aaTAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAAc                                                                                                                                                                                                        <  1:717276/251‑1 (MQ=255)                                  aaaTTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTa                                                                                                                                                                                       >  1:288985/1‑251 (MQ=255)                                     ttCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGAt                                                                                                                                                                                                              >  1:219288/1‑225 (MQ=255)                                     ttCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACaa                                                                                                                                                                                                      >  1:567014/1‑233 (MQ=255)                                       cctctAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTTGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAg                                                                                                                                                                                                                <  1:427284/221‑1 (MQ=255)                                        ctctaGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAaga                                                                                                                                                                                 >  1:551969/5‑251 (MQ=255)                                             gTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATa                                                                                                                                                                            >  1:692321/1‑251 (MQ=255)                                                  gCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGa                                                                                                                                                                       >  1:680147/1‑251 (MQ=255)                                                   cTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATgg                                                                                                                                                                        <  1:4919/249‑1 (MQ=255)                                                       agaAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATaa                                                                                                                                                                   <  1:793181/250‑1 (MQ=255)                                                         aaatcGATGTCTTCGTTTTTAACGTATCCGCTTCGGCGCCAAGTGCGGTCAGTTTTAACTATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATAACTTTAACCAATGAATGTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAAAAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGa                                                                                                                                                                 <  1:321469/246‑1 (MQ=255)                                                                 gTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATgg                                                                                                                                                                        >  1:161353/1‑235 (MQ=255)                                                                 gTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCg                                                                                                                                                        >  1:825035/1‑251 (MQ=255)                                                                        tttttAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATa                                                                                                                                                                                                                                  >  1:328932/1‑170 (MQ=255)                                                                        tttttAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCa                                                                                                                                                 >  1:808827/1‑251 (MQ=255)                                                                               cGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAAt                                                                                                                                                                          <  1:594377/219‑1 (MQ=255)                                                                                 tATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACa                                                                                                                                        <  1:361077/251‑1 (MQ=255)                                                                                   tCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAgg                                                                                                                                      <  1:623000/251‑1 (MQ=255)                                                                                            gCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAAttt                                                                                                                                                                                                                              >  1:187208/1‑154 (MQ=255)                                                                                             cACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATgg                                                                                                                                                                        <  1:218734/207‑1 (MQ=255)                                                                                              aCCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATa                                                                                                                                                                                                             <  1:104328/169‑1 (MQ=255)                                                                                                          cAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATgcg                                                                                                               >  1:331200/1‑251 (MQ=255)                                                                                                            gTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGAt                                                                                                                                                                                                              <  1:370184/154‑1 (MQ=255)                                                                                                            gTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACaataat                                                                                                                                                                                                  >  1:264797/1‑166 (MQ=255)                                                                                                            gTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATgg                                                                                                                                                                        >  1:624940/1‑192 (MQ=255)                                                                                                            gTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAAt                                                                                                                                                                     >  1:483569/1‑195 (MQ=255)                                                                                                              tttAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATAc                                                                                                                                         >  1:788007/1‑221 (MQ=255)                                                                                                                tAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCa                                                                                                         >  1:741374/1‑251 (MQ=255)                                                                                                                   cAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGaa                                                                                                      >  1:303344/1‑251 (MQ=255)                                                                                                                      tAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGac                                                                                                                                                                <  1:630819/190‑1 (MQ=255)                                                                                                                       aGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGAt                                                                                                                                                                                                              >  1:206327/1‑143 (MQ=255)                                                                                                                                  tttCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAAt                                                                                                                                                                     <  1:829952/173‑1 (MQ=255)                                                                                                                                        gTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAgg                                                                                                                                      <  1:784836/198‑1 (MQ=255)                                                                                                                                                   ctTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGaa                                                                      >  1:642871/1‑251 (MQ=255)                                                                                                                                                           aaaaTATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTg                                                              <  1:401258/251‑1 (MQ=255)                                                                                                                                                                atctttAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTa                                                          >  1:41426/4‑250 (MQ=255)                                                                                                                                                                 tctttAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATg                                                                                                                 >  1:528556/3‑194 (MQ=255)                                                                                                                                                                 tctttAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACg                                                        <  1:447188/249‑1 (MQ=255)                                                                                                                                                                 tctttAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACg                                                        >  1:256218/3‑251 (MQ=255)                                                                                                                                                                   tttAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGaa                                                      <  1:373488/251‑1 (MQ=255)                                                                                                                                                                                ttttCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGttt                                          >  1:586684/1‑250 (MQ=255)                                                                                                                                                                                ttttCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGttt                                          >  1:301311/1‑250 (MQ=255)                                                                                                                                                                                    cAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCAtt                                      >  1:63524/1‑250 (MQ=255)                                                                                                                                                                                          gTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGAt                                                                                                                                           >  1:39697/1‑143 (MQ=255)                                                                                                                                                                                          gTTTAATTTCTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTc                                     <  1:798591/245‑1 (MQ=255)                                                                                                                                                                                                   cTTTGCTTCTCCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATaa                      <  1:813005/251‑1 (MQ=255)                                                                                                                                                                                                          tctcCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGaaa               >  1:735535/1‑251 (MQ=255)                                                                                                                                                                                                           ctcCCGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAg              >  1:823944/1‑251 (MQ=255)                                                                                                                                                                                                             cccGCTTAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTtat                        <  1:135867/239‑1 (MQ=255)                                                                                                                                                                                                                   tAGTGCGAATTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAGTGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCAcatcat      <  1:110157/251‑1 (MQ=255)                                                                                                                                                                                                                      tgcgaaTTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATAc    <  1:98365/246‑1 (MQ=255)                                                                                                                                                                                                                      tgcgaaTTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACa   <  1:338307/247‑1 (MQ=255)                                                                                                                                                                                                                        cgaaTTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACaa  <  1:877449/248‑1 (MQ=255)                                                                                                                                                                                                                           aTTTTCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGcac           >  1:795241/1‑238 (MQ=255)                                                                                                                                                                                                                              ttCCTGAAGCGGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTCTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAATCGGAGAATCAAGCTGTTTACGaa                                                      >  1:23595/1‑192 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          TTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCCAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATGTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTGCTTCTCCCGCTTAGTGCAAATTTTCCTGAAGCCGTTGCGATAATTTCCACATTGCTAAAGATAGAAACAATAATCGCTAAAGTTAAAAAGAGCATAATGGAATAAGACACGAATCGTGGTGCATTTGATACAGGTGTTTCAATTAATTCCAAATGCGCGGGCAGAAATTCATTTTCATCTTTTTCTCTAACCGGAGAATCAAGCTGTTTACGAATTTTCCAAGTTTCATTCCACACGTTTTTATAACGGGAAAGCACATCATACAA  >  1/1976267‑1976731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑