Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,976,228	T→C	L175L (TTA→TTG)	unknown ←	RTX toxin transporter, determinant D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,976,228	0	T	C	100.0%	164.5 / NA	54	L175L (TTA→TTG)	unknown	RTX toxin transporter, determinant D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/26);  total (28/26)

ATCTAAAACTGTATGTTTTGCAATAGCATGTTCCTTATATAAGGCTCTAAAATCATCCAATCGGACTTGCTCTGTATTAATCAGACCTTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCCGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGTTCTTCAATCAGTAAAGTCACTCTTTTTTGATCGTTTTCCGTCATAAAAGGTAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCCAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATGTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTG  >  1/1975988‑1976465                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              aTCTAAAACCGTATGTTTTGCAATAGCATGTTCCTTATATAAGGCTCTAAAATCATCCAATCGGACTTGTTCTGTATTAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAAttt                                                                                                                                                                                                                                     >  1:711612/1‑251 (MQ=255) aTCTAAAACCGTATGTTTTGCAATAGCATGTTCCTTATATAAGGCTCTAAAATCATCCAATCGGACTTGTTCTGTATTAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAAttt                                                                                                                                                                                                                                     >  1:833953/1‑251 (MQ=255)        accgTATGTTTTGCAATAGCATGTTCCTTATATAAGGCTCTAAAATCATCCAATCGGACTTGTTCTGTATTAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGaaaa                                                                                                                                                                                                                              >  1:889527/4‑251 (MQ=255)                 tttGCAATAGCATGTTCCTTATATAAGGCTCTAAAATCATCCAATCGGACTTGTTCTGTATTAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAatat                                                                                                                                                                                                                     <  1:742546/251‑1 (MQ=255)                     cAATAGCATGTTCCTTATATAAGGCTCTAAAATCATCCAATCGGACTTGTTCTGTATTAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGt                                                                                                                                                                                                                 >  1:236792/1‑251 (MQ=255)                                tCCTTATATAAGGCTCTAAAATCATCCAATCGGACTTGTTCTGTATTAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAatat                                                                                                                                                                                                                     <  1:537445/236‑1 (MQ=255)                                     atataAGGCTCTAAAATCATCCAATCGGACTTGTTCTGTATTAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAatat                                                                                                                                                                                                                     <  1:416354/231‑1 (MQ=255)                                            gCTCTAAAATCATCCAATCGGACTTGTTCTGTATTAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAatat                                                                                                                                                                                                                     <  1:567329/224‑1 (MQ=255)                                                                gACTTGTTCTGTATTAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAAt                                                                                                                                                                                                              >  1:101900/1‑211 (MQ=255)                                                                     gttctGTATTAATCAAGCCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATc                                                                                                                                                                                                                                          <  1:11146/176‑1 (MQ=255)                                                                     gttctGTATTAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAAc                                                                                                                                                                                                <  1:215/218‑1 (MQ=255)                                                                     gttctGTATTAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATtc                                                                                                                                                                  >  1:772671/3‑250 (MQ=255)                                                                          gTATTAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATaa                                                                                                                                                                          <  1:688735/237‑1 (MQ=255)                                                                          gTATTAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATaa                                                                                                                                                                          <  1:282918/237‑1 (MQ=255)                                                                          gTATTAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATtcctctag                                                                                                                                                            >  1:529957/1‑248 (MQ=255)                                                                          gTATTAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATtcctctag                                                                                                                                                            >  1:635242/1‑248 (MQ=255)                                                                          gTATTAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATtcctctag                                                                                                                                                            >  1:186638/1‑248 (MQ=255)                                                                             ttAATCAAACCCTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGttt                                                                                                                                                         <  1:336917/251‑1 (MQ=255)                                                                                 tcaaaccctCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCt                                                                                                                                                     <  1:887829/243‑1 (MQ=255)                                                                                 tcaaaccctCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCt                                                                                                                                                     <  1:845734/243‑1 (MQ=255)                                                                                 tcaaaccctCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCt                                                                                                                                                     <  1:515300/243‑1 (MQ=255)                                                                                      ccctCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTgaga                                                                                                                                                >  1:7073/4‑251 (MQ=255)                                                                                       cctCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGaa                                                                                                                                               <  1:151377/249‑1 (MQ=255)                                                                                                  tttATTCGTGCCAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCtt                                                                                                                                    <  1:641919/251‑1 (MQ=255)                                                                                                             cAAATAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACg                                                                                                                         <  1:269433/251‑1 (MQ=255)                                                                                                                 tAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGaa                                                                                                                                                                                                                                <  1:144201/144‑1 (MQ=255)                                                                                                                 tAACTGAGTTTTTCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTAt                                                                                                                      >  1:638896/1‑250 (MQ=255)                                                                                                                          ttttCTGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGc                                                                                                            <  1:385931/251‑1 (MQ=255)                                                                                                                            ttctgCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAATTTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCAc                                                                                                          <  1:849074/247‑1 (MQ=255)                                                                                                                            ttctgCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCAc                                                                                                          <  1:52828/247‑1 (MQ=255)                                                                                                                                                gATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGa                                                                                                                                                                                 >  1:439747/1‑160 (MQ=255)                                                                                                                                                                aTGGCGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCtttt                                                                      >  1:618115/1‑251 (MQ=255)                                                                                                                                                                    cGTTGTTTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCAc                                                                  <  1:767103/251‑1 (MQ=255)                                                                                                                                                                        gttTTTGCCAAGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAAt                                                                                   >  1:106226/1‑230 (MQ=255)                                                                                                                                                                                 aaGTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCct                                                       >  1:277232/1‑249 (MQ=255)                                                                                                                                                                                   gTAGAGAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCttt                                                   >  1:412925/1‑251 (MQ=255)                                                                                                                                                                                     agagAATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGCTTTAACa                                                                                     >  1:139749/1‑215 (MQ=255)                                                                                                                                                                                       agaATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTcaca                                               >  1:704080/1‑251 (MQ=255)                                                                                                                                                                                        gaATTGCTCTTCAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACa                                                                                     <  1:301927/212‑1 (MQ=255)                                                                                                                                                                                             gctcttcAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCAc                                                                                                          <  1:835504/184‑1 (MQ=255)                                                                                                                                                                                                cttcAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAata                                                                                                                                                                                                                      <  1:347182/75‑1 (MQ=255)                                                                                                                                                                                                cttcAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAatatct                                      <  1:207713/251‑3 (MQ=255)                                                                                                                                                                                                  tcAATCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCAc                                                                  >  1:577571/1‑221 (MQ=255)                                                                                                                                                                                                      tCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCAccc                                                                          >  1:390519/1‑209 (MQ=255)                                                                                                                                                                                                      tCAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAAcc                                <  1:533356/251‑1 (MQ=255)                                                                                                                                                                                                       cAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCa                               >  1:93783/1‑251 (MQ=255)                                                                                                                                                                                                       cAGTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCa                               >  1:440516/1‑251 (MQ=255)                                                                                                                                                                                                         gTAAAGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAAt                             >  1:107242/1‑251 (MQ=255)                                                                                                                                                                                                             aGTCACCCTTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAatatct                                      >  1:56726/1‑236 (MQ=255)                                                                                                                                                                                                                  ccctTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGa                                                                                                                                                                                 >  1:421846/3‑94 (MQ=255)                                                                                                                                                                                                                    ctTTTTTGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCt                                                                                                                                                     <  1:800393/120‑1 (MQ=255)                                                                                                                                                                                                                      tttttGATCGTTTTCCGTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGATCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCg                >  1:429160/1‑251 (MQ=255)                                                                                                                                                                                                                                      gTCATAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATCTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTg  >  1:293067/1‑249 (MQ=255)                                                                                                                                                                                                                                         aTAAAAGGCAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCTAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAatatct                                      >  1:555634/1‑208 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              ATCTAAAACTGTATGTTTTGCAATAGCATGTTCCTTATATAAGGCTCTAAAATCATCCAATCGGACTTGCTCTGTATTAATCAGACCTTCATATTTTTTTATTCGTGCCAAATAACTGAGTTTTTCCGCTTCTTTTTTATTAAGATTCAAGGTTTTCTGATGGCGTTGTTTTTGCCAAGTAGAGAATTGTTCTTCAATCAGTAAAGTCACTCTTTTTTGATCGTTTTCCGTCATAAAAGGTAAATCAATTTTAGAAAAATCTAATATTGGTAATTGATCTTTTTCAACAGCTTCCAATAAAGATTTATAACGAAATTCCTCCAGTTTAGCTTGAGAAAGCGATGTCTTCGTTTTTAACGTATCCGCTTCGGCACCAAGTGCGGTCAGTTTTAACAATAGTTCACCCTTTTTCACGTATTCGCCCTCTTTCACAAAAATATGTTTAACCAATGAATTTTCAATCGGTTTAATTTCTTTG  >  1/1975988‑1976465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑