Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	292,550	T→C	*245Q (TAG→CAG)	unknown →	AAA+ ATPase superfamily protein YifB/ComM, associated with DNA recombination

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	292,550	0	T	C	100.0%	201.1 / NA	66	*245Q (TAG→CAG)	unknown	AAA+ ATPase superfamily protein YifB/ComM, associated with DNA recombination
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/38);  total (28/38)

GTAGTGCAATTTCTCAATAATCAGGACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGCGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACTAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAGTGGCTGAAAAGGAAGTGGATTATGTCATATTTCAACGAAAATAAATTAGAGAAAGTTCGCTGG  >  1/292304‑292764                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                       gtagtGCAATTTCTCAATAATCAGGACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGa                                                                                                                                                                                                                    >  1:234381/1‑251 (MQ=255)           ttCTCAATAATCAGGACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGaa                                                                                                                                                                                                          <  1:420133/251‑1 (MQ=255)            tctcAATAATCAGGACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAACATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACAGGAAATGACCGACCAGGAAGCCATTGaaa                                                                                                                                                                                                         >  1:300215/1‑251 (MQ=255)                        ggACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAg                                                                                                                                                                                             >  1:764884/1‑251 (MQ=255)                         gACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAg                                                                                                                                                                                             <  1:250724/250‑1 (MQ=255)                          aCGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGc                                                                                                                                                                                            <  1:483579/250‑1 (MQ=255)                                         ccGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAATAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAAc                                                                                                                                                                            <  1:519935/251‑1 (MQ=255)                                                      acaacaAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTAc                                                                                                                                                                                 >  1:834535/1‑233 (MQ=255)                                                         acaaAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATa                                                                                                                                                            >  1:879679/1‑251 (MQ=255)                                                            aaGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACt                                                                                                                                                         <  1:748215/251‑1 (MQ=255)                                                                      gATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCa                                                                                                                                                              >  1:292844/1‑236 (MQ=255)                                                                                aTGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATaa                                                                                                                                     >  1:339274/1‑251 (MQ=255)                                                                                   aaaaTCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTg                                                                                                                                  <  1:343694/251‑1 (MQ=255)                                                                                       tCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCaa                                                                                                                              >  1:509281/1‑251 (MQ=255)                                                                                                   cACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGaa                                                                                                                                                                                                          <  1:767968/163‑1 (MQ=255)                                                                                                   cACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAaata                                                                                                                  <  1:258693/251‑1 (MQ=255)                                                                                                   cACCGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGGCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAaata                                                                                                                  <  1:446605/251‑1 (MQ=255)                                                                                                     ccGATATTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAaataat                                                                                                                >  1:249772/1‑251 (MQ=255)                                                                                                        atatTATCGGTCAACAACACGCCAAACGTGCTCTCATTATCCCCGCCGCCGGCCACCACAATCTGTTCTTTCTCGGTCCTCCCGTCACCGGGACAACCATGCTTGCCTCCCCCTGAACCGGATTATTCCCGTAAATGACCGAGCACTCAGCCATTGAAACCTCGTCCGTACCCATTTAGGTACCACACTAACTGAATGTCCATAACTGGTGGTAAAATTGCAAATGACTTTCATACGATGCAAATAATTGt                                                                                                             >  1:518271/1‑251 (MQ=255)                                                                                                        atatTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAaata                                                                                                                  >  1:575320/1‑246 (MQ=255)                                                                                                        atatTATCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTg                                                                                                              <  1:415016/250‑1 (MQ=255)                                                                                                         tattatCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGt                                                                                                             >  1:74948/1‑250 (MQ=255)                                                                                                         tattatCGGTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGAACAGGAAGCCATTGAAACCGCGTCCTTAGCAATTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTaaaa                                                                                                                                               <  1:581270/216‑1 (MQ=255)                                                                                                                ggTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTtat                                                                                                     >  1:321165/1‑251 (MQ=255)                                                                                                                 gTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAAc                                                                                                                                                                            <  1:319035/179‑1 (MQ=255)                                                                                                                 gTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAAc                                                                                                                                    >  1:78195/1‑219 (MQ=255)                                                                                                                 gTCAACAACACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTtata                                                                                                    >  1:782112/1‑251 (MQ=255)                                                                                                                    aacaacACGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGCACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAAcc                                                                                                 <  1:309830/251‑1 (MQ=255)                                                                                                                     acaacaCGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAAccc                                                                                                <  1:687227/251‑1 (MQ=255)                                                                                                                           cGCCAAACGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAAc                                                                                                                                                                                                        >  1:593567/1‑141 (MQ=255)                                                                                                                                aaCGTGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAaata                                                                                                                  <  1:635910/222‑1 (MQ=255)                                                                                                                                  cgtgcTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATaa                                                                                   <  1:540610/248‑1 (MQ=255)                                                                                                                                   gtgcTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAAt                                                                                  >  1:633112/3‑251 (MQ=255)                                                                                                                                     gcTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAAc                                                                                                                                    <  1:140868/199‑1 (MQ=255)                                                                                                                                       tctcATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTAc                                                                              <  1:58569/251‑1 (MQ=255)                                                                                                                                        ctcATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTg                                                                                                                                                                      >  1:228485/1‑162 (MQ=255)                                                                                                                                        ctcATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAaata                                                                                                                  >  1:483495/1‑214 (MQ=255)                                                                                                                                        ctcATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTa                                                                               >  1:475741/1‑249 (MQ=255)                                                                                                                                          tggttttcccggtgccgGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAaata                                                                                                                  <  1:323011/199‑1 (MQ=255)                                                                                                                                               tCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTAt                                                                      >  1:820765/1‑251 (MQ=255)                                                                                                                                                  cAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTAttt                                                                    <  1:695226/250‑1 (MQ=255)                                                                                                                                                     ccgccgGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAg                                                                 <  1:574467/250‑1 (MQ=255)                                                                                                                                                     ccgccgGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAg                                                                 <  1:316137/250‑1 (MQ=255)                                                                                                                                                      cgccgGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAGTg                                                               <  1:882266/251‑1 (MQ=255)                                                                                                                                                          gggCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAAccc                                                                                                <  1:717521/214‑1 (MQ=255)                                                                                                                                                              aaCACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAGTGGCTGaaaa                                                       <  1:140829/251‑1 (MQ=255)                                                                                                                                                               acacAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAGTGGCTGAAAAg                                                      <  1:157212/251‑1 (MQ=255)                                                                                                                                                               acacAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAGTGGCTGAAAAg                                                      >  1:667043/1‑251 (MQ=255)                                                                                                                                                                  caATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATc                                                                                 <  1:506082/221‑1 (MQ=255)                                                                                                                                                                    aTCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAAccc                                                                                                <  1:422369/204‑1 (MQ=255)                                                                                                                                                                      cTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGa                                                                                                                          <  1:614147/176‑1 (MQ=255)                                                                                                                                                                      cTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATaa                                                                                   >  1:17850/1‑215 (MQ=255)                                                                                                                                                                        gTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGtt                                                                                                                                                                                       <  1:688122/113‑1 (MQ=255)                                                                                                                                                                        gTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTg                                                                                                              >  1:154771/1‑186 (MQ=255)                                                                                                                                                                        gTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAg                                                                 <  1:521285/231‑1 (MQ=255)                                                                                                                                                                        gTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAGt                                                                <  1:548754/232‑1 (MQ=255)                                                                                                                                                                             ttCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAAccc                                                                                                <  1:284527/195‑1 (MQ=255)                                                                                                                                                                                   gTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAGTGGCTGAAAAGGAAGTGGATTATGTCatat                                   <  1:301906/250‑1 (MQ=255)                                                                                                                                                                                       gCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAAc                                                                                                                                                                                                        <  1:248888/81‑1 (MQ=255)                                                                                                                                                                                       gCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATc                                                                                 >  1:695205/1‑200 (MQ=255)                                                                                                                                                                                                 gggAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAGTGGCTGAAAAGGAAGTGGATTATGTCATATTTCAACGAaaataaa                    >  1:114659/1‑251 (MQ=255)                                                                                                                                                                                                             cTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTg                                                                                                                                  <  1:474465/129‑1 (MQ=255)                                                                                                                                                                                                                     ccGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTAcc                                                                         <  1:311556/178‑1 (MQ=255)                                                                                                                                                                                                                     ccGTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAGTGGCTGAAAAGGAAGTGGATTATGTCATATTTCAACGAAAATAAATTAGAGAAAGTTCGCTgg  <  1:855294/249‑1 (MQ=255)                                                                                                                                                                                                                       gTTTAACCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAGACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAGTg                                                               >  1:543755/1‑186 (MQ=255)                                                                                                                                                                                                                            aCCGGATTATTACCGGAAATGACCGACCAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAGTGGCTGAAAAGGAAGTGGATTATGTCATATTTCAACGaaaa                        >  1:698679/1‑220 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                       GTAGTGCAATTTCTCAATAATCAGGACGAATTACCGCTTGCCGGTGAAATACCACAACAAAGTGCGGTGGATTTTTGCCATGAAAATCCGAAAGATCTCACCGATATTATCGGTCAACAACACGCCAAACGCGCTCTCATGATCGCAGCCGCCGGGCAACACAATCTGTTATTTCTCGGTCCGCCCGGCACCGGGAAAACCATGCTTGCCAGCCGTTTAACCGGATTATTACCGGAAATGACCGACTAGGAAGCCATTGAAACCGCGTCCGTAGCAAGTTTGGTACAAAACGAACTGAATTTTCATAACTGGTGTTAAAATTACAAATAACTGTCAAACGATACAAATAATTGTCGCCTTATAACCCAAATCATTTATAATCTACTTACCTATTTAAGTGGCTGAAAAGGAAGTGGATTATGTCATATTTCAACGAAAATAAATTAGAGAAAGTTCGCTGG  >  1/292304‑292764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑