Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,975,598	A→G	V385V (GTT→GTC)	unknown ←	RTX toxin transporter, determinant D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,975,598	0	A	G	100.0%	178.4 / NA	64	V385V (GTT→GTC)	unknown	RTX toxin transporter, determinant D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/25);  total (39/25)

AAGTAGATAGCTTATAACGCTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCGGAAAGTGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATCGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCCGTTAAATAACCATAACGGGTATA‑GGGGAAGGCTTCGACCTTAATTACAACCTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCAGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACTTTTAATTGTTGTACAGTACCTGATACAGGAGCTCGAATTTCAGAGACTTGCTGGCGTTGATTGTTTTTGTCCAGTTCGAATGTCAATAAATTCA  >  1/1975364‑1975824                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                   gaGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCcttctt                                                                                                                                                                                                                    >  1:764313/2‑251 (MQ=255)    tAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCttt                                                                                                                                                                                                                   <  1:466010/249‑1 (MQ=255)          gCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACaaaa                                                                                                                                                                                                            >  1:100005/1‑250 (MQ=255)          gCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACaaaa                                                                                                                                                                                                            >  1:249985/1‑250 (MQ=255)           cTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAAc                                                                                                                                                                                                           >  1:648739/1‑250 (MQ=255)                      tctcATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAata                                                                                                                                                                                                      <  1:732004/244‑1 (MQ=255)                      tctcATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTAt                                                                                                                                                                                               >  1:298106/1‑251 (MQ=255)                                gTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCCTCTTTCACAAAACCAATATCTTTATTTTGAATAAg                                                                                                                                                                                     >  1:166058/1‑251 (MQ=255)                                             cAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTc                                                                                                                                                                         <  1:508036/250‑1 (MQ=255)                                               gTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAg                                                                                                                                                                      >  1:860082/1‑251 (MQ=255)                                                  aTTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGa                                                                                                                                                                   >  1:354078/1‑251 (MQ=255)                                                    ttCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAAt                                                                                                                                                                 >  1:391497/1‑251 (MQ=255)                                                    ttCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAAt                                                                                                                                                                 >  1:842815/1‑251 (MQ=255)                                                         tCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAAtctt                                                                                                                                                              >  1:701280/1‑249 (MQ=255)                                                              gcagAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCgg                                                                                                                                                       >  1:108567/4‑251 (MQ=255)                                                                        ggATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTAc                                                                                                                                              >  1:51298/1‑250 (MQ=255)                                                                                           aGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCgtag                                                                                                                          >  1:238857/1‑251 (MQ=255)                                                                                                 tgtTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTaacaa                                                                                                                    <  1:623748/251‑1 (MQ=255)                                                                                                     tttttATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGc                                                                                                                 >  1:102838/1‑250 (MQ=255)                                                                                                     tttttATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGc                                                                                                                 >  1:48035/1‑250 (MQ=255)                                                                                                       tttATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCAt                                                                                                              <  1:569456/251‑1 (MQ=255)                                                                                                               aTTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATtgtg                                                                                                      <  1:609108/251‑1 (MQ=255)                                                                                                               aTTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATtgtg                                                                                                      <  1:194533/251‑1 (MQ=255)                                                                                                                  cAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAata                                                                                                                                                                                                      >  1:589950/1‑152 (MQ=255)                                                                                                                          gTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTcac                                                                                                                                                                                                                <  1:47527/134‑1 (MQ=255)                                                                                                                            aTTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGcgg                                                                                                                                                                                >  1:523631/1‑162 (MQ=255)                                                                                                                            aTTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAAtt                                                                                         >  1:781547/1‑251 (MQ=255)                                                                                                                            aTTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGGAGCCATAACGGGTATAGGGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATtgtgt                                                                                                     <  1:429193/240‑1 (MQ=255)                                                                                                                               aaaTACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATc                                                                                                                                                                                                    <  1:142416/141‑1 (MQ=255)                                                                                                                               aaaTACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTg                                                                                                                                                                                   >  1:868441/1‑158 (MQ=255)                                                                                                                                      aGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAAtt                                                                                         >  1:560632/1‑241 (MQ=255)                                                                                                                                       gTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAAcc                                                                                                >  1:9358/1‑232 (MQ=255)                                                                                                                                          cAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTAccc                                                                           <  1:575897/251‑2 (MQ=255)                                                                                                                                            aGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTg                                                                                                                                                                                   <  1:667851/145‑1 (MQ=255)                                                                                                                                             gCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAg                                                                                >  1:704373/1‑243 (MQ=255)                                                                                                                                              cTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGAt                                                                        <  1:13923/250‑1 (MQ=255)                                                                                                                                              cTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGAt                                                                        <  1:683002/250‑1 (MQ=255)                                                                                                                                                   gATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATtgt                                                                                                       >  1:770296/1‑214 (MQ=255)                                                                                                                                                    aTGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTGAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAggg                                                                  <  1:435212/250‑2 (MQ=255)                                                                                                                                                      gTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTATCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAgggg                                                                 >  1:256899/1‑247 (MQ=255)                                                                                                                                                      gTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAgggg                                                                 >  1:269427/1‑247 (MQ=255)                                                                                                                                                      gTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAgg                                                                   <  1:703151/247‑1 (MQ=255)                                                                                                                                                           aTCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAGGGGCTCGAAt                                                          <  1:204569/251‑1 (MQ=255)                                                                                                                                                               cATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAggg                                                                                                                                                                    <  1:894491/141‑1 (MQ=255)                                                                                                                                                               cATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAgg                                                                   <  1:614661/238‑1 (MQ=255)                                                                                                                                                                                          ctgTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAggg                                                                                                                                                                    <  1:677137/112‑1 (MQ=255)                                                                                                                                                                                            gTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGATGTCTTGCCCTTCTTTCACAAAACCACTATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGACCCTTTAATTGCTGTACAGTACCCGATAc                                                                      >  1:287122/1‑206 (MQ=255)                                                                                                                                                                                                    gccATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAGGGGCTCGAATTTCAGAGACTTGCTGGCGTTGATTGTTTTTGTCCAGCTCGa                 >  1:221135/2‑251 (MQ=255)                                                                                                                                                                                                         aaCGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAGGGGCTCGAATTTCAGAGACTTGCTGGCGTTGATTGTTTTTGTCCAGCTCGAATGt             >  1:443795/1‑250 (MQ=255)                                                                                                                                                                                                           cGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAGGGGCTCGAATTTCAGAGACTTGCTGGCGTTGATTGTTTTTGTCCAGCTCGAATGTCaa          >  1:150321/1‑251 (MQ=255)                                                                                                                                                                                                            gggTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATcagaaaacc                                                                                                     <  1:213584/159‑9 (MQ=255)                                                                                                                                                                                                             ggTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATg                                                                                                                                      >  1:699227/1‑125 (MQ=255)                                                                                                                                                                                                             ggTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGAt                                                                        >  1:182960/1‑187 (MQ=255)                                                                                                                                                                                                              gTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAGGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAGGGGCTCGAATTTCAGAGACTTGCTGGCGTTGATTGTTTTTGTCCAGCTCGAATGTCAATaa       >  1:76974/1‑251 (MQ=255)                                                                                                                                                                                                              gTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAGGGGCTCGAATTTCAGAGACTTGCTGGCGTTGATTGTTTTTGTCCAGCTCGAATGTCAATaa       >  1:119013/1‑251 (MQ=255)                                                                                                                                                                                                                ata‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAGGGGCTCGAATTTCAGAGACTTGCTGGCGTTGATTGTTTTTGTCCAGCTCGAATGTCAATAAAt     >  1:233036/1‑251 (MQ=255)                                                                                                                                                                                                                ata‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAGGGGCTCGAATTTCAGAGACTTGCTGGCGTTGATTGTTTTTGTCCAGCTCGAATGTCAATAAAt     >  1:756900/1‑251 (MQ=255)                                                                                                                                                                                                                ata‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAGGGGCTCGAATTTCAGAGACTTGCTGGCGTTGATTGTTTTTGTCCAGCTCGAATGTCAATAAAt     >  1:64578/1‑251 (MQ=255)                                                                                                                                                                                                                    ggggAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAGGGGCTCGAATTTCAGAGACTTGCTGGCGTTGATTGTTTTTGTCCAGCTCGAATGTCAATAAATTca  >  1:846557/1‑251 (MQ=255)                                                                                                                                                                                                                                     tAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATc                                                                                                                                                                                                    <  1:806959/40‑1 (MQ=255)                                                                                                                                                                                                                                      aaTTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAGGGGCTCGAATTTCAGAGACTTGCTGGCGTTGATTg                                 <  1:351074/202‑1 (MQ=255)                                                                                                                                                                                                                                       aTTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGAt                                                                        <  1:528020/162‑1 (MQ=255)                                                                                                                                                                                                                                       aTTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAGGGGCTCGAATTTCAGAGACTTGCTGGCGTTGATTGTTTTTGTCCAGCTCGAATg              >  1:831769/1‑220 (MQ=255)                                                                                                                                                                                                                                       aTTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCAATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGAt                                                                        <  1:708684/162‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                   AAGTAGATAGCTTATAACGCTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCGGAAAGTGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATCGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCCGTTAAATAACCATAACGGGTATA‑GGGGAAGGCTTCGACCTTAATTACAACCTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCAGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACTTTTAATTGTTGTACAGTACCTGATACAGGAGCTCGAATTTCAGAGACTTGCTGGCGTTGATTGTTTTTGTCCAGTTCGAATGTCAATAAATTCA  >  1/1975364‑1975824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑