Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,975,556	A→G	Y399Y (TAT→TAC)	unknown ←	RTX toxin transporter, determinant D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,975,556	0	A	G	100.0%	183.6 / NA	60	Y399Y (TAT→TAC)	unknown	RTX toxin transporter, determinant D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/26);  total (34/26)

TTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTAAGTAGATAGCTTATAACGCTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCGGAAAGTGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATCGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCCGTTAAATAACCATAACGGGTATA‑GGGGAAGGCTTCGACCTTAATTACAACCTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCAGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACTTTTAATTGTTGTACAGTACCTGATACAGGAGCTCGAAT  >  1/1975319‑1975768                                                                                                                                                                                                                                              |                                                                                                                                                                                                                      ttAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACggg                                                                                                                                                                                                          >  1:868852/1‑251 (MQ=255)                     tATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAAcc                                                                                                                                                                                     <  1:486646/251‑1 (MQ=255)                        cAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑gggg                                                                                                                                                                                                 <  1:503733/236‑1 (MQ=255)                               ttcttcTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTAcga                                                                                                                                                                           <  1:291475/251‑3 (MQ=255)                                 cttctAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑ggg                                                                                                                                                                                                  >  1:20637/1‑226 (MQ=255)                                              gaGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCcttctt                                                                                                                                                            >  1:764313/2‑251 (MQ=255)                                                 tAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCttt                                                                                                                                                           <  1:466010/249‑1 (MQ=255)                                                       gCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTcaac                                                                                                                                                                                      >  1:570023/1‑213 (MQ=255)                                                       gCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTAcgacgtc                                                                                                                                                                       >  1:165696/1‑225 (MQ=255)                                                       gCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACaaaa                                                                                                                                                    >  1:100005/1‑250 (MQ=255)                                                       gCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACaaaa                                                                                                                                                    >  1:249985/1‑250 (MQ=255)                                                        cTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑gggg                                                                                                                                                                                                 >  1:562379/1‑204 (MQ=255)                                                        cTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAAc                                                                                                                                                   >  1:648739/1‑250 (MQ=255)                                                            taACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑gggg                                                                                                                                                                                                 <  1:326662/200‑1 (MQ=255)                                                                   tctcATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑gggg                                                                                                                                                                                                 <  1:778622/193‑1 (MQ=255)                                                                   tctcATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAata                                                                                                                                              <  1:732004/244‑1 (MQ=255)                                                                   tctcATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTAt                                                                                                                                       >  1:298106/1‑251 (MQ=255)                                                                             gTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCCTCTTTCACAAAACCAATATCTTTATTTTGAATAAg                                                                                                                             >  1:166058/1‑251 (MQ=255)                                                                                    tttCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTa                                                                                                                                                                              <  1:349133/195‑1 (MQ=255)                                                                                          cAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTc                                                                                                                 <  1:508036/250‑1 (MQ=255)                                                                                            gTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAg                                                                                                              >  1:860082/1‑251 (MQ=255)                                                                                               aTTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGa                                                                                                           >  1:354078/1‑251 (MQ=255)                                                                                                 ttCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAAt                                                                                                         >  1:842815/1‑251 (MQ=255)                                                                                                 ttCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAAt                                                                                                         >  1:391497/1‑251 (MQ=255)                                                                                                      tCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACg                                                                                                                                                                                                            >  1:242093/1‑148 (MQ=255)                                                                                                      tCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAAtctt                                                                                                      >  1:701280/1‑249 (MQ=255)                                                                                                           gcagAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCgg                                                                                               >  1:108567/4‑251 (MQ=255)                                                                                                                     ggATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTAc                                                                                      >  1:51298/1‑250 (MQ=255)                                                                                                                                        aGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCgtag                                                                  >  1:238857/1‑251 (MQ=255)                                                                                                                                              tgtTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTaacaa                                                            <  1:623748/251‑1 (MQ=255)                                                                                                                                                  tttttATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGc                                                         >  1:102838/1‑250 (MQ=255)                                                                                                                                                  tttttATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGc                                                         >  1:48035/1‑250 (MQ=255)                                                                                                                                                    tttATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCAt                                                      <  1:569456/251‑1 (MQ=255)                                                                                                                                                            aTTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATtgtg                                              <  1:609108/251‑1 (MQ=255)                                                                                                                                                            aTTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATtgtg                                              <  1:194533/251‑1 (MQ=255)                                                                                                                                                               cAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAata                                                                                                                                              >  1:589950/1‑152 (MQ=255)                                                                                                                                                                       gTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTcac                                                                                                                                                        <  1:47527/134‑1 (MQ=255)                                                                                                                                                                         aTTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑gggg                                                                                                                                                                                                 >  1:516449/1‑91 (MQ=255)                                                                                                                                                                         aTTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGcgg                                                                                                                        >  1:523631/1‑162 (MQ=255)                                                                                                                                                                         aTTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAAtt                                 >  1:781547/1‑251 (MQ=255)                                                                                                                                                                         aTTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGGAGCCATAACGGGTATAGGGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATtgtgt                                             <  1:429193/240‑1 (MQ=255)                                                                                                                                                                            aaaTACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATc                                                                                                                                            <  1:142416/141‑1 (MQ=255)                                                                                                                                                                            aaaTACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTg                                                                                                                           >  1:868441/1‑158 (MQ=255)                                                                                                                                                                                   aGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAAtt                                 >  1:560632/1‑241 (MQ=255)                                                                                                                                                                                    gTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAAcc                                        >  1:9358/1‑232 (MQ=255)                                                                                                                                                                                       cAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTAccc                   <  1:575897/251‑2 (MQ=255)                                                                                                                                                                                         aGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTg                                                                                                                           <  1:667851/145‑1 (MQ=255)                                                                                                                                                                                          gCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAg                        >  1:704373/1‑243 (MQ=255)                                                                                                                                                                                           cTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGAt                <  1:683002/250‑1 (MQ=255)                                                                                                                                                                                           cTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGAt                <  1:13923/250‑1 (MQ=255)                                                                                                                                                                                                gATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATtgt                                               >  1:770296/1‑214 (MQ=255)                                                                                                                                                                                                 aTGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTGAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAggg          <  1:435212/250‑2 (MQ=255)                                                                                                                                                                                                   gTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTATCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAgggg         >  1:256899/1‑247 (MQ=255)                                                                                                                                                                                                   gTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAgggg         >  1:269427/1‑247 (MQ=255)                                                                                                                                                                                                   gTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAgg           <  1:703151/247‑1 (MQ=255)                                                                                                                                                                                                        aTCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAGGGGCTCGAAt  <  1:204569/251‑1 (MQ=255)                                                                                                                                                                                                            cATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAggg                                                                                                            <  1:894491/141‑1 (MQ=255)                                                                                                                                                                                                            cATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACCTTTAATTGCTGTACAGTACCCGATACAgg           <  1:614661/238‑1 (MQ=255)                                                                                                                                                                                                                                       ctgTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGACGTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCGGTGACTTCAAggg                                                                                                            <  1:677137/112‑1 (MQ=255)                                                                                                                                                                                                                                         gTTAAGTAGCCATAACGGGTATA‑GGGGAAGGCTTCAACCTTAATTACGATGTCTTGCCCTTCTTTCACAAAACCACTATCTTTATTTTGAATAAGTGCGGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGACCCTTTAATTGCTGTACAGTACCCGATAc              >  1:287122/1‑206 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                      TTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTAAGTAGATAGCTTATAACGCTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCGGAAAGTGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATCGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCCGTTAAATAACCATAACGGGTATA‑GGGGAAGGCTTCGACCTTAATTACAACCTCTTGCCCTTCTTTCACAAAACCAATATCTTTATTTTGAATAAGTGCAGTGACTTCAAGGGAATCTTCTTCCGGCACAACTACCATTAATGTTTCAGCCGTAGTAACAACGCCATCTATTGTGTGAACTTTTAATTGTTGTACAGTACCTGATACAGGAGCTCGAAT  >  1/1975319‑1975768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑