Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,975,364	A→G	L463L (CTT→CTC)	unknown ←	RTX toxin transporter, determinant D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,975,364	0	A	G	100.0%	110.2 / NA	39	L463L (CTT→CTC)	unknown	RTX toxin transporter, determinant D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/21);  total (18/21)

CACTTGAATTTTTGACCGCACTTTTGC‑CCCGAAAACCTACAACGGGCTTTCCACTTGCAGGCGTTCGCAAATGCCGGTAATGCGCTTTCAATTATAAATCCATCATCAAGTCACAGTTTTATACTGCCTAACCAAATCTATTTTCAATTTAGACTTTTGATACATTGTGGAAAACCGGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTAAGTAGATAGCTTATAACGCTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCGGAAAGTGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATCGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCCGTTAAATAACCATAACGGGTATAGGGGAAGGCTTCGACCTTAATTACAA  >  1/1975117‑1975599                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            caccggggaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGt                                                                                                                                                                                                                                          <  1:429082/237‑1 (MQ=255)        gaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAg                                                                                                                                                                                                                                    >  1:884751/8‑250 (MQ=255)         aaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGc                                                                                                                                                                                                                                   <  1:207804/244‑1 (MQ=255)               aCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                                                                                                                                                                                                                            <  1:443109/251‑1 (MQ=255)               aCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                                                                                                                                                                                                                            <  1:296055/251‑1 (MQ=255)                           caccagAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGcc                                                                                                                                                                                                                <  1:873801/246‑1 (MQ=255)                                      cTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAAtt                                                                                                                                                                                                      <  1:214057/251‑1 (MQ=255)                                      cTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAAtt                                                                                                                                                                                                      <  1:295554/251‑1 (MQ=255)                                        aCAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAAttt                                                                                                                                                                                                     >  1:881110/1‑250 (MQ=255)                                                     ggcttgctggtattCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                                                                                                                                                                                                                            <  1:751097/202‑1 (MQ=255)                                                     ggcttgctggtattCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGcc                                                                                                                                                                                                                >  1:338020/13‑226 (MQ=255)                                                                                                taAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAgtg                                                                                                                                            <  1:142307/251‑1 (MQ=255)                                                                                                         catcaAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGccc                                                                                                                                                                                                               <  1:157105/175‑2 (MQ=255)                                                                                                                               gtcTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTaaa                                                                                                             >  1:773845/3‑251 (MQ=255)                                                                                                                                             aTTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                                                                                                                                                                                                                            <  1:448433/126‑1 (MQ=255)                                                                                                                                                              ttGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGcc                                                                                                                                                                                                                >  1:795488/1‑121 (MQ=255)                                                                                                                                                                               atcagAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGa                                                                                          <  1:695966/218‑1 (MQ=255)                                                                                                                                                                                tcagAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATAc                                                                                                          <  1:820679/202‑1 (MQ=255)                                                                                                                                                                                       gTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACtttt                                                      >  1:653710/1‑250 (MQ=255)                                                                                                                                                                                          atatTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAg                                                                                                                                                                                                                                        >  1:112360/1‑69 (MQ=255)                                                                                                                                                                                          atatTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTc                                                                                                     >  1:690566/1‑200 (MQ=255)                                                                                                                                                                                            atTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGtt                                                >  1:785456/1‑251 (MQ=255)                                                                                                                                                                                                 aTCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCTTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAAt                                                                                                            >  1:823336/1‑186 (MQ=255)                                                                                                                                                                                                         taATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGt                                                                      <  1:825647/216‑1 (MQ=255)                                                                                                                                                                                                           aTTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAg                                                                       <  1:308747/213‑1 (MQ=255)                                                                                                                                                                                                            ttAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACggg                                >  1:868852/1‑251 (MQ=255)                                                                                                                                                                                                                    ctctTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                                                                                                                                                                                                                            >  1:339050/1‑55 (MQ=255)                                                                                                                                                                                                                    ctctTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATAc                                                                                                          <  1:114736/169‑1 (MQ=255)                                                                                                                                                                                                                    ctctTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAatat                                                                 <  1:635134/210‑1 (MQ=255)                                                                                                                                                                                                                          aaCTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATc                                                                                 <  1:31486/188‑1 (MQ=255)                                                                                                                                                                                                                              tttATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGtttttt                                                                                                                                      >  1:461315/1‑131 (MQ=255)                                                                                                                                                                                                                                tATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCaataa                                                                                                                       >  1:65170/1‑144 (MQ=255)                                                                                                                                                                                                                                tATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATAGGGGAAGGCTTCAAcc            <  1:486646/251‑1 (MQ=255)                                                                                                                                                                                                                                   cAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATAgggg                        <  1:503733/236‑1 (MQ=255)                                                                                                                                                                                                                                          ttcttcTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATAGGGGAAGGCTTCAACCTTAATTAcga  <  1:291475/251‑3 (MQ=255)                                                                                                                                                                                                                                            cttctAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTcc                                                                                                                                                                                       >  1:73516/1‑68 (MQ=255)                                                                                                                                                                                                                                            cttctAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCaataa                                                                                                                       >  1:223849/1‑132 (MQ=255)                                                                                                                                                                                                                                            cttctAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTc                                                                                                     >  1:84242/1‑150 (MQ=255)                                                                                                                                                                                                                                            cttctAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCTGTTAAGTAGCCATAACGGGTATAggg                         >  1:20637/1‑226 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                            CACTTGAATTTTTGACCGCACTTTTGC‑CCCGAAAACCTACAACGGGCTTTCCACTTGCAGGCGTTCGCAAATGCCGGTAATGCGCTTTCAATTATAAATCCATCATCAAGTCACAGTTTTATACTGCCTAACCAAATCTATTTTCAATTTAGACTTTTGATACATTGTGGAAAACCGGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTAAGTAGATAGCTTATAACGCTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCGGAAAGTGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATCGTATTAAATACAAGTCCAAGCTTTGGATGTTCAATCGCATCTAAAGTAATATTTTTCACTTTTCCCGTTAAATAACCATAACGGGTATAGGGGAAGGCTTCGACCTTAATTACAA  >  1/1975117‑1975599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑