BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,975,284	T→C	intergenic (+235/+35)	unknown → / ← unknown	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)/RTX toxin transporter, determinant D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,975,284	0	T	C	100.0%	55.9 / NA	21	intergenic (+235/+35)	unknown/unknown	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)/RTX toxin transporter, determinant D
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (8/13); total (8/13)

CACTTGAATTTTTGACCGCACTTTTGC‑CCCGAAAACCTACAACGGGCTTTCCACTTGCAGGCGTTCGCAAATGCCGGTAATGCGCTTTCAATTATAAATCCATCATCAAGTCACAGTTTTATACTGCCTAACCAAATCTATTTTCAATTTAGACTTTTGATACATTGTGGAAAACCGGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTAAGTAGATAGCTTATAACGCTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCGGAAAGTGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATCGTATTAAA  >  1/1975117‑1975492

caccggggaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGt                                                                                                                               <  1:429082/237‑1 (MQ=255)

     gggaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTtat                                                                                                                                                                                  >  1:699651/10‑195 (MQ=255)

       gaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAg                                                                                                                         >  1:884751/8‑250 (MQ=255)

        aaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGc                                                                                                                        <  1:207804/244‑1 (MQ=255)

              aCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                                                                                                                 <  1:443109/251‑1 (MQ=255)

              aCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                                                                                                                 <  1:296055/251‑1 (MQ=255)

                    cTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTc                                                                                                                                                                                            <  1:845446/170‑1 (MQ=255)

                          caccagAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGcc                                                                                                     <  1:873801/246‑1 (MQ=255)

                                     cTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAAtt                                                                                           <  1:295554/251‑1 (MQ=255)

                                     cTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAAtt                                                                                           <  1:214057/251‑1 (MQ=255)

                                       aCAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAAttt                                                                                          >  1:881110/1‑250 (MQ=255)

                                                    ggcttgctggtattCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                                                                                                                 <  1:751097/202‑1 (MQ=255)

                                                    ggcttgctggtattCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGcc                                                                                                     >  1:338020/13‑226 (MQ=255)

                                                                                           aTTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTatat                                                                                                                                                                                              <  1:663152/98‑1 (MQ=255)

                                                                                               taAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAgtg                                 <  1:142307/251‑1 (MQ=255)

                                                                                                        catcaAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGccc                                                                                                    <  1:157105/175‑2 (MQ=255)

                                                                                                                              gtcTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCtt                                                                                                                                                                                      >  1:188589/3‑71 (MQ=255)

                                                                                                                              gtcTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCAGAAAGCGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATTGTATTaaa  >  1:773845/3‑251 (MQ=255)

                                                                                                                                            aTTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                                                                                                                 <  1:448433/126‑1 (MQ=255)

                                                                                                                                                          cTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGc                                                                                                                                    >  1:309974/1‑93 (MQ=255)

                                                                                                                                                             ttGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGcc                                                                                                     >  1:795488/1‑121 (MQ=255)

CACTTGAATTTTTGACCGCACTTTTGC‑CCCGAAAACCTACAACGGGCTTTCCACTTGCAGGCGTTCGCAAATGCCGGTAATGCGCTTTCAATTATAAATCCATCATCAAGTCACAGTTTTATACTGCCTAACCAAATCTATTTTCAATTTAGACTTTTGATACATTGTGGAAAACCGGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTAAGTAGATAGCTTATAACGCTTCTCATGCCTGTCTTAATTTCTGCAGTAATTTCCATCCCTGCGGAAAGTGGGATTTCTTTTTCTTCGGTAGAAAGTGTTTTTTTATCCAATTCAATAATCGTATTAAA  >  1/1975117‑1975492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`