Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,975,201	C→A	intergenic (+152/+118)	unknown → / ← unknown	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)/RTX toxin transporter, determinant D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,975,201	0	C	A	100.0%	36.8 / NA	14	intergenic (+152/+118)	unknown/unknown	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)/RTX toxin transporter, determinant D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (5/9);  total (5/9)

CACTTGAATTTTTGACCGCACTTTTGC‑CCCGAAAACCTACAACGGGCTTTCCACTTGCAGGCGTTCGCAAATGCCGGTAATGCGCTTTCAATTATAAATCCATCATCAAGTCACAGTTTTATACTGCCTAACCAAATCTATTTTCAATTTAGACTTTTGATACATTGTGGAAAACCGGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTAAGTAGATAGCTTATAACGCTTCTCATGCCTGTCTTAATTT  >  1/1975117‑1975404                                                                                      |                                                                                                                                                                                                            caccggggaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGt                                       <  1:429082/237‑1 (MQ=255)      gggaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGAt                                                                                                                                 >  1:572457/10‑156 (MQ=255)      gggaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTtat                                                                                          >  1:699651/10‑195 (MQ=255)        gaaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAg                                 >  1:884751/8‑250 (MQ=255)         aaatccaCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGc                                <  1:207804/244‑1 (MQ=255)               aCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                         <  1:296055/251‑1 (MQ=255)               aCCGCACTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                         <  1:443109/251‑1 (MQ=255)                     cTTTTGCACCAGAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTc                                                                                                    <  1:845446/170‑1 (MQ=255)                           caccagAAAA‑CTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGcc             <  1:873801/246‑1 (MQ=255)                                      cTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAAtt   <  1:214057/251‑1 (MQ=255)                                      cTACAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAAtt   <  1:295554/251‑1 (MQ=255)                                        aCAACGGGCTTTCGGCTTGCTGGTATTCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGCCTGTCTTAAttt  >  1:881110/1‑250 (MQ=255)                                                     ggcttgctggtattCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAAc                         <  1:751097/202‑1 (MQ=255)                                                     ggcttgctggtattCGCAAATGCAGGTAATACGATTTCAATTATAAATCCATCATCAAGTTACCGTTTTATACTGTCTAACCAAATCCATTTTCAATTTAGACTTTTGATACATTGCGGAAAATCAGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTGAGTAGATAGCTTATAACACTTCTCATGcc             >  1:338020/13‑226 (MQ=255)                                                                                      |                                                                                                                                                                                                            CACTTGAATTTTTGACCGCACTTTTGC‑CCCGAAAACCTACAACGGGCTTTCCACTTGCAGGCGTTCGCAAATGCCGGTAATGCGCTTTCAATTATAAATCCATCATCAAGTCACAGTTTTATACTGCCTAACCAAATCTATTTTCAATTTAGACTTTTGATACATTGTGGAAAACCGGAATGTTATATTCTATCTTTTATAATTAACGTTCTCTTAAACTTTTATCAATAGATTCTTCTAATGGGCTAAGTAGATAGCTTATAACGCTTCTCATGCCTGTCTTAATTT  >  1/1975117‑1975404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑