Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,974,902	T→C	D288D (GAT→GAC)	unknown →	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,974,902	0	T	C	100.0%	138.1 / NA	47	D288D (GAT→GAC)	unknown	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/21);  total (26/21)

AAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCAATCGGTGTGATCGACAACAGCCGCAAAGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGCAATGATACCGATTCCACCGCCGTGTATTCCTGTGAAGCGGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTAGAACGCTATATGCAATCTTTCGCCGATGAAATGGCGTGCTTTGTGGATTCCGTAGTAAACGACAAACCGACTTTAGTCAACGGCAACGACGGCTTGCAACCTGTGCTGATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCT‑AAAGCATTTTCTGATGCT‑AAAAAAGACCTGAAACCGATTCAGGTCTTTTGTTTTTCGG‑TGATTAGAAAACACTTGAATTTT  >  1/1974657‑1975128                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                      aaaGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAAt                                                                                                                                                                                                                                  <  1:65615/251‑1 (MQ=255)         cGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGc                                                                                                                                                                                                                           <  1:639506/250‑1 (MQ=255)            tatTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTtg                                                                                                                                                                                                                       >  1:5097/1‑251 (MQ=255)             atTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTtgt                                                                                                                                                                                                                      >  1:57163/1‑251 (MQ=255)                 acacCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGa                                                                                                                                                                                                                   <  1:378297/250‑1 (MQ=255)                           catcaCCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCgtagtga                                                                                                                                                                                                        <  1:42308/251‑3 (MQ=255)                             tcaCCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAAc                                                                                                                                                                                                      <  1:445439/251‑1 (MQ=255)                                               aTGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTc                                                                                                                                                                                    <  1:360193/251‑1 (MQ=255)                                               aTGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTc                                                                                                                                                                                    <  1:379670/251‑1 (MQ=255)                                                tGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAg                                                                                                                                                                                      <  1:546951/248‑1 (MQ=255)                                                         cGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAacg                                                                                                                                                                          >  1:596554/1‑251 (MQ=255)                                                           gtgtAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCgtag                                                                                                                                                                                                           <  1:500796/216‑1 (MQ=255)                                                           gtgtAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAacga                                                                                                                                                                         >  1:690386/1‑250 (MQ=255)                                                             gtaaTCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACgg                                                                                                                                                                      >  1:734006/4‑251 (MQ=255)                                                                     caacaGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAAc                                                                                                                                                              <  1:414053/251‑1 (MQ=255)                                                                      aacaGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAAcc                                                                                                                                                             <  1:61599/251‑1 (MQ=255)                                                                            cGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCt                                                                                                                                                       <  1:553317/251‑1 (MQ=255)                                                                                aggCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCttat                                                                                                                                                    >  1:177835/3‑247 (MQ=255)                                                                                aggCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGGACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCttat                                                                                                                                                    >  1:644201/3‑247 (MQ=255)                                                                                                    cAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCgtagtga                                                                                                                                                                                                        >  1:387036/1‑176 (MQ=255)                                                                                                             gAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTtgatga                                                                                                                      >  1:823111/1‑251 (MQ=255)                                                                                                                       gCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCa                                                                                                                                                                                   <  1:224060/180‑1 (MQ=255)                                                                                                                                     gTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGa                                                                                              >  1:880564/1‑251 (MQ=255)                                                                                                                                     gTACAAACCCGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGa                                                                                              >  1:185426/1‑251 (MQ=255)                                                                                                                                            cccGTAATGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGc                                                                                        >  1:487487/1‑250 (MQ=255)                                                                                                                                               gtaaTGATACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCT‑aa                                                                                   >  1:887019/3‑251 (MQ=255)                                                                                                                                                      tACCGATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCAtt                                                                             >  1:443160/1‑251 (MQ=255)                                                                                                                                                          gATTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCAtttccg                                                                         >  1:503848/1‑248 (MQ=255)                                                                                                                                                           aTTCTACCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCAtttccga                                                                        >  1:846995/1‑247 (MQ=255)                                                                                                                                                            ttctaCCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTc                                                                                                                                                                                    <  1:134680/138‑1 (MQ=255)                                                                                                                                                            ttctaCCGCCGTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCAtttccgat                                                                       >  1:513850/5‑246 (MQ=255)                                                                                                                                                                     cgTGTATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGAT‑aaaaaa                                                            <  1:396822/251‑1 (MQ=255)                                                                                                                                                                      gtgtATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGcc                                                                                                              <  1:705786/202‑1 (MQ=255)                                                                                                                                                                       tgtATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTCATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGAT‑aaaaaaag                                                          <  1:49216/251‑3 (MQ=255)                                                                                                                                                                         tATTCCTGTGAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGAT‑aaaaaaagac                                                        <  1:349131/251‑5 (MQ=255)                                                                                                                                                                               tgtgAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGaag                                                   <  1:135529/251‑2 (MQ=255)                                                                                                                                                                               tgtgAAGCAGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGaag                                                   <  1:393287/251‑2 (MQ=255)                                                                                                                                                                                                   cGAAAAACCGAAATATTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGttt                               <  1:858422/251‑1 (MQ=255)                                                                                                                                                                                                                tatTTCTTCCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCggcgggttaa                  >  1:539021/1‑243 (MQ=255)                                                                                                                                                                                                                       tcCTGGAACGCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCggcgggttaaaaaaca            >  1:485296/1‑236 (MQ=255)                                                                                                                                                                                                                                gCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGaagcgaattc                                            >  1:814593/1‑201 (MQ=255)                                                                                                                                                                                                                                gCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAAtttt  >  1:418922/1‑251 (MQ=255)                                                                                                                                                                                                                                gCTATATGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAGCA‑TTTCCGATGATAAAAAAAGACCTGAAGCGAATTCAGGTCTTTTGTTTTTCGGCGGGTTAAAAAACACTTGAAtttt  >  1:496082/1‑251 (MQ=255)                                                                                                                                                                                                                                   atatGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAAc                                                                                                                                                              >  1:597368/1‑93 (MQ=255)                                                                                                                                                                                                                                   atatGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCTAAAAACA‑TTTCCGATGATAAAAAAAGACCTGa                                                     >  1:452156/1‑197 (MQ=255)                                                                                                                                                                                                                                    tatGCAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCtt                                                                                                                                                      >  1:863097/1‑99 (MQ=255)                                                                                                                                                                                                                                        cAATCTTTCGCTGACGAAATGGCGTGCTTTGTGGATTCCGTAGTGAACGACAAACCGACTTTAGTCAACGGCAACGACGGTTTGCAACCTGTGCTTATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAg                                                                                                                    >  1:426257/1‑130 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                      AAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCAATCGGTGTGATCGACAACAGCCGCAAAGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGCAATGATACCGATTCCACCGCCGTGTATTCCTGTGAAGCGGGCGTGATTGCCGAAAAACCGAAATATTTCTTCCTAGAACGCTATATGCAATCTTTCGCCGATGAAATGGCGTGCTTTGTGGATTCCGTAGTAAACGACAAACCGACTTTAGTCAACGGCAACGACGGCTTGCAACCTGTGCTGATCGCGTTGGCGGCAAAACGTTCTCTTGATGAAGGACGCCCGGTGAAACTGGCGGAAATCGCCT‑AAAGCATTTTCTGATGCT‑AAAAAAGACCTGAAACCGATTCAGGTCTTTTGTTTTTCGG‑TGATTAGAAAACACTTGAATTTT  >  1/1974657‑1975128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑