Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	287,760	C→T	G176G (GGC→GGT)	unknown →	Methionine ABC transporter permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	287,760	0	C	T	100.0%	190.7 / NA	66	G176G (GGC→GGT)	unknown	Methionine ABC transporter permease protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/37);  total (29/37)

CACCACTGCCGCCATTATTCCGCTGAGTATTTGTGCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGCGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACACTGACATAAAAAGGGGATCTTTTATGAACTTGAAAAAATTATTAGGCGTCGCAACATTAGCCTCCGTATTCGCCTTAACGG  >  1/287520‑288002                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   caccacTGCCGCCATTATTCCGCTGAGTATTTGTGCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCt                                                                                                                                                                                                                                          <  1:494189/251‑1 (MQ=255)   ccacTGCCGCCATTATTCCGCTGAGTATTTGTGCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTa                                                                                                                                                                                                                                        >  1:276453/1‑251 (MQ=255)     acTGCCGCCATTATTCCGCTGAGTATTTGTGCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAgg                                                                                                                                                                                                                                      >  1:258801/1‑251 (MQ=255)        gccgccATTATTCCGCTGAGTATTTGTGCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAg                                                                                                                                                                                                                                   >  1:200529/1‑251 (MQ=255)            ccATTATTCCGCTGAGTATTTGTGCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCtt                                                                                                                                                                                                                               >  1:587053/1‑251 (MQ=255)            ccATTATTCCGCTGAGTATTTGTGCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCtt                                                                                                                                                                                                                               >  1:563611/1‑251 (MQ=255)              attattCCGCTGAGTATTTGTGCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTg                                                                                                                                                                                                                              >  1:586841/1‑250 (MQ=255)                 attCCGCTGAGTATTTGTGCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCAt                                                                                                                                                                                                                          >  1:166946/1‑251 (MQ=255)                     cGCTGAGTATTTGTGCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAAc                                                                                                                                                                                                                      <  1:387105/251‑1 (MQ=255)                     cGCTGAGTATTTGTGCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAAc                                                                                                                                                                                                                      <  1:233082/251‑1 (MQ=255)                         gAGTATTTGTGCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGcc                                                                                                                                                                                                                                    >  1:694094/1‑232 (MQ=255)                           gTATTTGTGCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTa                                                                                                                                                                                                                    <  1:91047/247‑1 (MQ=255)                           gTATTTGTGCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACggg                                                                                                                                                                                                                >  1:360472/1‑250 (MQ=255)                             aTTTGTGCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGa                                                                                                                                                                                                              <  1:276889/251‑1 (MQ=255)                                 gtgCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAatat                                                                                                                                                                                                          >  1:717889/1‑251 (MQ=255)                                 gtgCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGGGAatat                                                                                                                                                                                                          >  1:869361/1‑251 (MQ=255)                                      aTGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCaa                                                                                                                                                                                                     <  1:361214/251‑1 (MQ=255)                                          cGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCaactgg                                                                                                                                                                                                 >  1:640382/1‑247 (MQ=255)                                            tttGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGt                                                                                                                                                                                                                >  1:554621/1‑234 (MQ=255)                                            tttGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGAc                                                                                                                                                                                               <  1:355222/251‑1 (MQ=255)                                                     cgTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTaa                                                                                                                                                                                       <  1:139552/250‑1 (MQ=255)                                                            ccGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACt                                                                                                                                                                                                                     <  1:390248/213‑1 (MQ=255)                                                                      cTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCTCCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTGAAATGGGTCGCCACCatta                                                                                                                                                                     <  1:523171/251‑1 (MQ=255)                                                                           ttGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCattattat                                                                                                                                                                 <  1:615693/250‑1 (MQ=255)                                                                            tGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATtg                                                                                                                                                               <  1:68316/251‑1 (MQ=255)                                                                               aaTTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTgg                                                                                                                                                            <  1:319462/251‑1 (MQ=255)                                                                               aaTTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTgg                                                                                                                                                            <  1:634621/251‑1 (MQ=255)                                                                                         ggCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAat                                                                                                                                                                                                            <  1:734832/193‑1 (MQ=255)                                                                                         ggCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCAt                                                                                                                                                  >  1:670164/1‑251 (MQ=255)                                                                                            ttAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGAt                                                                                                                                               <  1:418676/251‑1 (MQ=255)                                                                                                        gcaAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGAcc                                                                                                                                                                                              >  1:214083/1‑192 (MQ=255)                                                                                                        gcaAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAAt                                                                                                                                                                                    >  1:826016/1‑202 (MQ=255)                                                                                                                  tGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGAc                                                                                                                         <  1:288892/251‑1 (MQ=255)                                                                                                                        ccaccaATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGc                                                                                                                    <  1:219971/250‑1 (MQ=255)                                                                                                                        ccaccaATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCa                                                                                                                   >  1:312072/1‑251 (MQ=255)                                                                                                                             aaTTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACg                                                                                                              <  1:288755/251‑1 (MQ=255)                                                                                                                                              tAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCgttagt                                                                                             <  1:635415/251‑1 (MQ=255)                                                                                                                                                  ttCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAAc                                                                                         <  1:67774/251‑1 (MQ=255)                                                                                                                                                         tGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTcacat                                                                                  <  1:330385/251‑2 (MQ=255)                                                                                                                                                               aaGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACa                                                                             <  1:213332/250‑1 (MQ=255)                                                                                                                                                                aGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGCGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATa                                                                           >  1:131792/1‑251 (MQ=255)                                                                                                                                                                    ttGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATaaaaa                                                                       <  1:62905/251‑1 (MQ=255)                                                                                                                                                                     tGCCAACACTCATTAACGGCATCGCCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGGCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTGCGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAg                                                                      <  1:396917/251‑1 (MQ=255)                                                                                                                                                                             cTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTc                                                                                      >  1:416600/1‑227 (MQ=255)                                                                                                                                                                             cTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCtt                                                              >  1:57707/1‑251 (MQ=255)                                                                                                                                                                             cTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCtt                                                              <  1:829554/251‑1 (MQ=255)                                                                                                                                                                             cTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCtt                                                              <  1:695731/251‑1 (MQ=255)                                                                                                                                                                               cATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGatc                                                                                                      <  1:676620/209‑1 (MQ=255)                                                                                                                                                                               cATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAg                                                                      >  1:732337/1‑241 (MQ=255)                                                                                                                                                                               cATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCtttt                                                            >  1:610897/1‑251 (MQ=255)                                                                                                                                                                                             cccTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGt                                                                                                                                                                                         <  1:138106/112‑1 (MQ=255)                                                                                                                                                                                             cccTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCTTTTATGAACTTGaaaaa                                              >  1:459222/1‑251 (MQ=255)                                                                                                                                                                                             cccTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCTTTTATGAACTTGaaaaa                                              >  1:350103/1‑251 (MQ=255)                                                                                                                                                                                                      tGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCTTTTATGAACTTGAAAAAATTATTAgg                                     <  1:317999/251‑1 (MQ=255)                                                                                                                                                                                                      tGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGACAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCTTTTATGAACTTGAAAAAATTATTAgg                                     <  1:783082/251‑1 (MQ=255)                                                                                                                                                                                                       ggTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGc                                                                                                                                                                                                       >  1:495420/1‑88 (MQ=255)                                                                                                                                                                                                            cccTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTc                                                                                                                                                       >  1:640251/1‑131 (MQ=255)                                                                                                                                                                                                              cTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCTTTTATGAACTTGAAAAAATTATTAGGCGTCGCaa                             >  1:351145/1‑251 (MQ=255)                                                                                                                                                                                                                      cTATTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCTTTTATGAACTTGAAAAAATTATTAGGCGTCGCAACATTAGcc                     <  1:22772/251‑1 (MQ=255)                                                                                                                                                                                                                        aTTCCGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCTTTTATGAAc                                                      <  1:625370/216‑1 (MQ=255)                                                                                                                                                                                                                            cGCCATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCTTTTATGAACTTGAAAAAATTATTAGGCGTCGCAACATTAGCCTCCGTa               >  1:462350/1‑251 (MQ=255)                                                                                                                                                                                                                              ccATGGCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCTTTTATGAACTTGAAAAAATTATTAGGCGTCGCAACATTAGCCTCCGTAt              <  1:513538/250‑1 (MQ=255)                                                                                                                                                                                                                                   gCAGGTATCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCTTTTATGAACTTGAAAAAATTATTAGGCGTCGCAACATTAGCCTCCGTATTCGc          >  1:345022/1‑249 (MQ=255)                                                                                                                                                                                                                                         aTCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCTTTTATGAACTTGAAAAAATTATTAGGCGTCGCAACATTAGCCTCCGTATTCGCCTTAACgg  <  1:541636/251‑1 (MQ=255)                                                                                                                                                                                                                                         aTCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCTTTTATGAACTTGAAAAAATTATTAGGCGTCGCAACATTAGCCTCCGTATTCGCCTTAACgg  <  1:736111/251‑1 (MQ=255)                                                                                                                                                                                                                                         aTCGTCGGTGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACATTGACATAAAAAGGGGATCTTTTATGAACTTGAAAAAATTATTAGGCGTCGCAACATTAGCCTCCGTATTCGCCTTAACgg  <  1:74061/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   CACCACTGCCGCCATTATTCCGCTGAGTATTTGTGCCATGCCGTTTGTGGCGCGTTTAACCGCCAACGCCTTGGTTGAAATTCCAAAAGGCTTAACGGAAGCAGCAAAAGCCATGGGCGCCACCAATTGGCAGATTATCCGTAAATTCTATTTGCCGGAAGCTTTGCCAACACTCATTAACGGCATCACCCTGACGCTGGTCACCCTTGTGGGCTATTCCGCCATGGCAGGTATCGTCGGCGGCGGTGGCTTAGGCAGCCTTGCCATTAACTACGGTGAATATCGCAATATGACCTATGTAAAATGGGTCGCCACCATTATTATTGTGGTATTCGTCATGATTTCACAAAAACTTGGCGATGACTTGGCAAAACGTGTCGATCATCGTTAGTTAACTTCACACTGACATAAAAAGGGGATCTTTTATGAACTTGAAAAAATTATTAGGCGTCGCAACATTAGCCTCCGTATTCGCCTTAACGG  >  1/287520‑288002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑