Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,974,569	C→T	H177H (CAC→CAT)	unknown →	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,974,569	0	C	T	100.0%	204.7 / NA	71	H177H (CAC→CAT)	unknown	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/34);  total (37/34)

CCGGTGGATGCCGATGTGAATCGCATTAAAGAAGTCTTAGTCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGTGAACCGCACTTAATCCGCGTTACCTCACGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTATCCAGCGGGATGTTCTTTGACATGACCATTCACGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTGGTAGAAGTGTACGCGGCGGGCGGCGTGCTGGTGAATCCTGAAATCGGCAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCAATCGGTGTGATCGACAACAGCCGCAAAGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGCAATGATACCGATT  >  1/1974327‑1974813                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     ccGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGa                                                                                                                                                                                                                                              >  1:887966/1‑251 (MQ=255) ccGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGa                                                                                                                                                                                                                                              >  1:516256/1‑251 (MQ=255)    gTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACa                                                                                                                                                                                                                                            <  1:674570/250‑1 (MQ=255)    gTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACa                                                                                                                                                                                                                                            <  1:374561/250‑1 (MQ=255)        aTGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGAt                                                                                                                                                                                                                                        >  1:678989/1‑250 (MQ=255)              aTGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGtta                                                                                                                                                                                                                                  <  1:492441/250‑1 (MQ=255)                gtaaaTCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGttattt                                                                                                                                                                                                                               >  1:667743/4‑251 (MQ=255)                    aTCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATcc                                                                                                                                                                                                                           <  1:94359/251‑1 (MQ=255)                        cATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGAt                                                                                                                                                                                                                                                   >  1:790278/1‑223 (MQ=255)                        cATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGAt                                                                                                                                                                                                                                                   >  1:303309/1‑223 (MQ=255)                        cATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGc                                                                                                                                                                                                                        <  1:556919/250‑1 (MQ=255)                            aaaGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGa                                                                                                                                                                                                                   >  1:563730/1‑251 (MQ=255)                                  gTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGtcgt                                                                                                                                                                                                             >  1:482041/1‑248 (MQ=255)                                  ctCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGtcgt                                                                                                                                                                                                             >  1:653461/2‑248 (MQ=255)                                      tagccGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCTCAACTTTAAAGCCATCAAGTCCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGtcgtggaa                                                                                                                                                                                                         <  1:218988/247‑8 (MQ=255)                                      tagccGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGtcgtggaa                                                                                                                                                                                                         >  1:655093/5‑244 (MQ=255)                                         ccGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAgtg                                                                                                                                                                                                      <  1:139677/250‑1 (MQ=255)                                              gTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTatg                                                                                                                                                                                                  >  1:721195/1‑248 (MQ=255)                                                 gAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGAGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATgcgg                                                                                                                                                                                               >  1:431605/1‑250 (MQ=255)                                                                aaaTTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTgg                                                                                                                                                                                <  1:553543/250‑1 (MQ=255)                                                                                                  acaACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGac                                                                                                                                             <  1:826627/251‑1 (MQ=255)                                                                                                   caACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGaca                                                                                                                                            >  1:342681/1‑251 (MQ=255)                                                                                                     aCTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGacac                                                                                                                                           >  1:882184/1‑250 (MQ=255)                                                                                                     aCTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGacac                                                                                                                                           >  1:328400/1‑250 (MQ=255)                                                                                                      cTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATAtt                                                                                                                                                >  1:233628/1‑244 (MQ=255)                                                                                                      cTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACAccg                                                                                                                                         >  1:836599/1‑251 (MQ=255)                                                                                                        ttAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACAccgcc                                                                                                                                       <  1:247862/251‑1 (MQ=255)                                                                                                               cATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGtcatca                                                                                                                                >  1:223551/1‑251 (MQ=255)                                                                                                                aTCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGaca                                                                                                                                            <  1:566258/238‑1 (MQ=255)                                                                                                                    aGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTa                                                                                                                           >  1:639976/1‑251 (MQ=255)                                                                                                                     gACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTaa                                                                                                                          >  1:52184/1‑251 (MQ=255)                                                                                                                            gTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTgg                                                                                                                    >  1:169640/1‑250 (MQ=255)                                                                                                                                              ggcgAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGGGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGgtgg                                                                                                 >  1:676035/4‑250 (MQ=255)                                                                                                                                                    ccGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGa                                                                                           >  1:827804/1‑251 (MQ=255)                                                                                                                                                       cACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGac                                                                                                                                             >  1:671614/1‑198 (MQ=255)                                                                                                                                                       cACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGgtgtaat                                                                                              <  1:157099/245‑4 (MQ=255)                                                                                                                                                            aaTCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAGTCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGc                                                                                    >  1:451556/1‑250 (MQ=255)                                                                                                                                                                     caCCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAg                                                                                                                                                                                                                 <  1:594688/115‑1 (MQ=255)                                                                                                                                                                          cccgcgACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTa                                                                      >  1:594154/3‑250 (MQ=255)                                                                                                                                                                                agccTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTa                                                                >  1:142619/3‑250 (MQ=255)                                                                                                                                                                                       cGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCaa                                                         >  1:851316/1‑250 (MQ=255)                                                                                                                                                                                                 aTTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAg                                               <  1:564885/250‑1 (MQ=255)                                                                                                                                                                                                 aTTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAg                                               <  1:115840/250‑1 (MQ=255)                                                                                                                                                                                                    gAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGc                                                                                    <  1:800144/210‑1 (MQ=255)                                                                                                                                                                                                        aCGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGttatttat                                                                                                                                                                                                                             <  1:249125/69‑1 (MQ=255)                                                                                                                                                                                                        aCGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGc                                       <  1:803942/251‑1 (MQ=255)                                                                                                                                                                                                         cGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGGCACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCgg                                        >  1:656870/1‑249 (MQ=255)                                                                                                                                                                                                         cGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCgg                                        >  1:211775/1‑249 (MQ=255)                                                                                                                                                                                                         cGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGGGTTGGAGAATCCGGAAATCGGTAAAACCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGGGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGGTCGGc                                       >  1:441463/1‑250 (MQ=255)                                                                                                                                                                                                           ttAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTc                                     <  1:845510/250‑1 (MQ=255)                                                                                                                                                                                                              aaGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGaaa                                 <  1:842736/251‑1 (MQ=255)                                                                                                                                                                                                                gtgtccggCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAgg                               >  1:204705/8‑251 (MQ=255)                                                                                                                                                                                                                        cGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTAc                       <  1:727847/251‑1 (MQ=255)                                                                                                                                                                                                                         gggATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCg                                                                                            >  1:606487/1‑181 (MQ=255)                                                                                                                                                                                                                         gggATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACggg                                                     <  1:521151/220‑1 (MQ=255)                                                                                                                                                                                                                             tGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAAcc                  <  1:688104/251‑1 (MQ=255)                                                                                                                                                                                                                             tGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAAcc                  <  1:208491/251‑1 (MQ=255)                                                                                                                                                                                                                             tGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAAcc                  <  1:132471/251‑1 (MQ=255)                                                                                                                                                                                                                                tCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATTCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGtcatc                                                                                                                                 >  1:649999/1‑137 (MQ=255)                                                                                                                                                                                                                                tCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAAc                                                                                                                       >  1:77146/1‑147 (MQ=255)                                                                                                                                                                                                                                tCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCg                                                                                            <  1:413891/174‑1 (MQ=255)                                                                                                                                                                                                                                tCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGAcc                                                           <  1:125163/207‑1 (MQ=255)                                                                                                                                                                                                                                  tttGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCgtaa             <  1:395590/251‑4 (MQ=255)                                                                                                                                                                                                                                    tGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATg           <  1:873218/251‑1 (MQ=255)                                                                                                                                                                                                                                    tGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATg           <  1:445639/251‑1 (MQ=255)                                                                                                                                                                                                                                    tGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATg           <  1:599622/251‑1 (MQ=255)                                                                                                                                                                                                                                    tGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTCACACCGCCGTCATCAACTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATg           <  1:270088/251‑1 (MQ=255)                                                                                                                                                                                                                                     gACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGa          >  1:883658/1‑251 (MQ=255)                                                                                                                                                                                                                                        aTGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATAc       <  1:390884/251‑1 (MQ=255)                                                                                                                                                                                                                                             cATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATACCGAtt  <  1:405952/251‑1 (MQ=255)                                                                                                                                                                                                                                              aTTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGCCGCAAGGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGTAATGATAc       >  1:863885/1‑245 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     CCGGTGGATGCCGATGTGAATCGCATTAAAGAAGTCTTAGTCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGTGAACCGCACTTAATCCGCGTTACCTCACGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTATCCAGCGGGATGTTCTTTGACATGACCATTCACGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTGGTAGAAGTGTACGCGGCGGGCGGCGTGCTGGTGAATCCTGAAATCGGCAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCAATCGGTGTGATCGACAACAGCCGCAAAGCGGTTTACGGCTACGACCAACGGGCGGAAGTGTTCGGCTCGAAAGGCGCGGTACAAACCCGCAATGATACCGATT  >  1/1974327‑1974813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑