Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,974,497	A→C	S153S (TCA→TCC)	unknown →	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,974,497	0	A	C	100.0%	176.9 / NA	62	S153S (TCA→TCC)	unknown	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/31);  total (31/31)

TCTTCCACAAACACCCACGCACCGATTTCCATTGAAGCCGCTCGTGCCGGCAAACACATTTTCTGTGAAAAACCGGTGGATGCCGATGTGAATCGCATTAAAGAAGTCTTAGTCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGTGAACCGCACTTAATCCGCGTTACCTCACGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTATCCAGCGGGATGTTCTTTGACATGACCATTCACGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTGGTAGAAGTGTACGCGGCGGGCGGCGTGCTGGTGAATCCTGAAATCGGCAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCAATCGGTGTGATCGACAACAGC  >  1/1974255‑1974731                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           tcctcCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGAcc                                                                                                                                                                                                                                     >  1:104962/4‑250 (MQ=255)      caccaacgcccACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACg                                                                                                                                                                                                                               <  1:177208/243‑1 (MQ=255)      caccaaCACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACg                                                                                                                                                                                                                               <  1:74194/247‑1 (MQ=255)      caccaaCACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACg                                                                                                                                                                                                                               <  1:31671/247‑1 (MQ=255)         caaCACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCAc                                                                                                                                                                                                                            <  1:562509/250‑1 (MQ=255)            cacCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCAccgc                                                                                                                                                                                                                         <  1:700079/251‑1 (MQ=255)                    cgccGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGa                                                                                                                                                                                                                  <  1:515912/248‑1 (MQ=255)                       cGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATAc                                                                                                                                                                                                              <  1:787050/251‑1 (MQ=255)                       cGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATAc                                                                                                                                                                                                              <  1:726700/251‑1 (MQ=255)                          tttCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGtt                                                                                                                                                                                                           <  1:886349/251‑1 (MQ=255)                          tttCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGtt                                                                                                                                                                                                           <  1:613826/251‑1 (MQ=255)                             ccATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTaaa                                                                                                                                                                                                        >  1:782928/1‑251 (MQ=255)                                  gAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGtgt                                                                                                                                                                                                    >  1:596399/1‑248 (MQ=255)                                             tGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATg                                                                                                                                                                                         <  1:565819/250‑1 (MQ=255)                                                   cAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCttt                                                                                                                                                                                   <  1:303974/250‑1 (MQ=255)                                                                  cgAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCatg                                                                                                                                                                   <  1:748338/250‑3 (MQ=255)                                                                         ccGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGa                                                                                                                                                            >  1:516256/1‑251 (MQ=255)                                                                         ccGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGa                                                                                                                                                            >  1:887966/1‑251 (MQ=255)                                                                            gTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGAc                                                                                                                                                                                <  1:42577/228‑1 (MQ=255)                                                                            gTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACa                                                                                                                                                          <  1:674570/250‑1 (MQ=255)                                                                            gTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACa                                                                                                                                                          <  1:374561/250‑1 (MQ=255)                                                                                aTGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGc                                                                                                                                                                                                                              <  1:28313/178‑1 (MQ=255)                                                                                aTGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGAt                                                                                                                                                      >  1:678989/1‑250 (MQ=255)                                                                                      aTGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGtta                                                                                                                                                <  1:492441/250‑1 (MQ=255)                                                                                        gtaaaTCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGttattt                                                                                                                                             >  1:667743/4‑251 (MQ=255)                                                                                            aTCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATcc                                                                                                                                         <  1:94359/251‑1 (MQ=255)                                                                                                cATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGAt                                                                                                                                                                 >  1:303309/1‑223 (MQ=255)                                                                                                cATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGAt                                                                                                                                                                 >  1:790278/1‑223 (MQ=255)                                                                                                cATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGc                                                                                                                                      <  1:556919/250‑1 (MQ=255)                                                                                                  ttAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGc                                                                                                                                                                                                                              <  1:188643/160‑1 (MQ=255)                                                                                                    aaaGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGa                                                                                                                                 >  1:563730/1‑251 (MQ=255)                                                                                                          gTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGtcgt                                                                                                                           >  1:482041/1‑248 (MQ=255)                                                                                                          ctCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGtcgt                                                                                                                           >  1:653461/2‑248 (MQ=255)                                                                                                              tagccGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCTCAACTTTAAAGCCATCAAGTCCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGtcgtggaa                                                                                                                       <  1:218988/247‑8 (MQ=255)                                                                                                              tagccGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGtcgtggaa                                                                                                                       >  1:655093/5‑244 (MQ=255)                                                                                                                gccGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACg                                                                                                                                                                                                                               <  1:100882/143‑1 (MQ=255)                                                                                                                 ccGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAgtg                                                                                                                    <  1:139677/250‑1 (MQ=255)                                                                                                                      gTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTatg                                                                                                                >  1:721195/1‑248 (MQ=255)                                                                                                                         gAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACg                                                                                                                                                                                                                               <  1:387692/136‑1 (MQ=255)                                                                                                                         gAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGAGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATgcgg                                                                                                             >  1:431605/1‑250 (MQ=255)                                                                                                                                        aaaTTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTgg                                                                                              <  1:553543/250‑1 (MQ=255)                                                                                                                                                                          acaACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGac                                                           <  1:826627/251‑1 (MQ=255)                                                                                                                                                                           caACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGaca                                                          >  1:342681/1‑251 (MQ=255)                                                                                                                                                                             aCTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGacac                                                         >  1:328400/1‑250 (MQ=255)                                                                                                                                                                             aCTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGacac                                                         >  1:882184/1‑250 (MQ=255)                                                                                                                                                                              cTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATAtt                                                              >  1:233628/1‑244 (MQ=255)                                                                                                                                                                              cTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACAccg                                                       >  1:836599/1‑251 (MQ=255)                                                                                                                                                                                ttAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACAccgcc                                                     <  1:247862/251‑1 (MQ=255)                                                                                                                                                                                       cATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGtcatca                                              >  1:223551/1‑251 (MQ=255)                                                                                                                                                                                        aTCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAg                                                                                                                                                                                                       >  1:863922/1‑97 (MQ=255)                                                                                                                                                                                        aTCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCatg                                                                                                                                                                   >  1:384382/1‑131 (MQ=255)                                                                                                                                                                                        aTCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGaca                                                          <  1:566258/238‑1 (MQ=255)                                                                                                                                                                                            aGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTa                                         >  1:639976/1‑251 (MQ=255)                                                                                                                                                                                             gACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTaa                                        >  1:52184/1‑251 (MQ=255)                                                                                                                                                                                                    gTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTgg                                  >  1:169640/1‑250 (MQ=255)                                                                                                                                                                                                                      ggcgAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGGGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGgtgg               >  1:676035/4‑250 (MQ=255)                                                                                                                                                                                                                            ccGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGa         >  1:827804/1‑251 (MQ=255)                                                                                                                                                                                                                               cACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACa                                                                                                                                                                               >  1:243295/1‑82 (MQ=255)                                                                                                                                                                                                                               cACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGac                                                           >  1:671614/1‑198 (MQ=255)                                                                                                                                                                                                                               cACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAATCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGgtgtaat            <  1:157099/245‑4 (MQ=255)                                                                                                                                                                                                                                    aaTCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTCGTGGAAGTGTATGCGGCGGGTGGCGTGTTGGTGAATCCGGAAGTCGGTAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCGATCGGTGTAATCGACAACAGc  >  1:451556/1‑250 (MQ=255)                                                                                                                                                                                                                                             caCCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCTTTGACATGACCATTCATGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAg                                                                                                                               <  1:594688/115‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           TCTTCCACAAACACCCACGCACCGATTTCCATTGAAGCCGCTCGTGCCGGCAAACACATTTTCTGTGAAAAACCGGTGGATGCCGATGTGAATCGCATTAAAGAAGTCTTAGTCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGTGAACCGCACTTAATCCGCGTTACCTCACGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTATCCAGCGGGATGTTCTTTGACATGACCATTCACGATTTCGACATGATCCGTTATTTATCCGGCAGCGAAGTGGTAGAAGTGTACGCGGCGGGCGGCGTGCTGGTGAATCCTGAAATCGGCAAAGCCGGCGATATTGACACCGCCGTCATCACCTTAAAACTGGCGAATGGCGCAATCGGTGTGATCGACAACAGC  >  1/1974255‑1974731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑