Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,974,320	T→C	C94C (TGT→TGC)	unknown →	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,974,320	0	T	C	100.0%	206.3 / NA	70	C94C (TGT→TGC)	unknown	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/35);  total (35/35)

GTCGCGTGCATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCACACAATGGGCGAAAGGCATGGGCATTCCACACGTTTATGAAGACTACAAACACATTCTGCAGGATCCTGAAATTGATGCTGTGTTGGTTTGTTCTTCCACAAACACCCACGCACCGATTTCCATTGAAGCCGCTCGTGCCGGCAAACACATTTTCTGTGAAAAACCGGTGGATGCCGATGTGAATCGCATTAAAGAAGTCTTAGTCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGTGAACCGCACTTAATCCGCGTTACCTCACGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTATCCAGCGGGATGTTCTTT  >  1/1974076‑1974554                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           gTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGaaaaa                                                                                                                                                                                                                                      >  1:162760/1‑251 (MQ=255) gTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGaaaaa                                                                                                                                                                                                                                      >  1:594170/1‑251 (MQ=255)          aTTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGAt                                                                                                                                                                                                                             <  1:326067/251‑1 (MQ=255)                 aaGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGAtg                                                                                                                                                                                                                      <  1:700048/251‑1 (MQ=255)                    cATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGAtgtaa                                                                                                                                                                                                                   >  1:513249/1‑249 (MQ=255)                      ttACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAAt                                                                                                                                                                                                                 <  1:330953/251‑1 (MQ=255)                                     aaGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTc                                                                                                                                                                                                  <  1:866012/251‑1 (MQ=255)                                            tGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAgccg                                                                                                                                                                                           <  1:383919/251‑4 (MQ=255)                                               aaTCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTc                                                                                                                                                                                                  >  1:356828/1‑241 (MQ=255)                                                    aaGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGaa                                                                                                                                                                                   <  1:461379/251‑1 (MQ=255)                                                         aTTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGc                                                                                                                                                                              <  1:198963/251‑1 (MQ=255)                                                                                          caATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAg                                                                                                                                                                               >  1:547738/1‑217 (MQ=255)                                                                                          caATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACcgtcg                                                                                                                                             <  1:204822/251‑1 (MQ=255)                                                                                          caATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACcgtcg                                                                                                                                             >  1:341536/1‑251 (MQ=255)                                                                                                   aaaGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGAcc                                                                                                                                     <  1:587679/250‑1 (MQ=255)                                                                                                      ggCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAGTCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACaa                                                                                                                                 >  1:441467/1‑251 (MQ=255)                                                                                                      ggCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACaa                                                                                                                                 >  1:362919/1‑251 (MQ=255)                                                                                                        cATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAAc                                                                                                                                <  1:404763/250‑1 (MQ=255)                                                                                                              cATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAg                                                                                                                         <  1:806970/251‑1 (MQ=255)                                                                                                               aTTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAg                                                                                                                         >  1:93938/1‑250 (MQ=255)                                                                                                                ttCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGc                                                                                                                        >  1:289581/1‑250 (MQ=255)                                                                                                                      acGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAg                                                                                                                 <  1:848513/251‑1 (MQ=255)                                                                                                                       cGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGAcc                                                                                                                                     >  1:67125/1‑230 (MQ=255)                                                                                                                       cGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGa                                                                                                                >  1:666265/1‑251 (MQ=255)                                                                                                                         tttATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGCTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGc                                                                                                                        >  1:753810/1‑241 (MQ=255)                                                                                                                           tATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGAcc                                                                                                              <  1:348367/249‑1 (MQ=255)                                                                                                                                 gACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAg                                                                                                                         <  1:821504/232‑1 (MQ=255)                                                                                                                                   ctataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGc                                                                                                                        >  1:784753/5‑231 (MQ=255)                                                                                                                                   ctataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGAc                                                                                                               <  1:709802/236‑1 (MQ=255)                                                                                                                                   ctataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGaaa                                                                                                    >  1:868873/5‑251 (MQ=255)                                                                                                                                   ctataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGaaa                                                                                                    >  1:669229/5‑251 (MQ=255)                                                                                                                                   ctataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGaaa                                                                                                    >  1:63145/5‑251 (MQ=255)                                                                                                                                     ataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGaaaa                                                                                                   >  1:100089/3‑250 (MQ=255)                                                                                                                                     ataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGaaaa                                                                                                   >  1:338905/3‑250 (MQ=255)                                                                                                                                                ttGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGAt                                                                                                                                                       >  1:512368/2‑187 (MQ=255)                                                                                                                                                       aTCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGTGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAg                                                                                                                 <  1:454166/218‑1 (MQ=255)                                                                                                                                                       aTCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCg                                                                                >  1:762072/1‑251 (MQ=255)                                                                                                                                                        tCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCg                                                                                >  1:109358/1‑250 (MQ=255)                                                                                                                                                         ccTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGAcc                                                                                                              >  1:367340/1‑219 (MQ=255)                                                                                                                                                         ccTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCa                                                                              >  1:261634/1‑251 (MQ=255)                                                                                                                                                              aaTTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGAcc                                                                                                              <  1:364457/214‑1 (MQ=255)                                                                                                                                                              aaTTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCggcga                                                                                    <  1:200208/240‑4 (MQ=255)                                                                                                                                                              aaTTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTaa                                                                         >  1:19409/1‑251 (MQ=255)                                                                                                                                                                ttGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATc                                                                       <  1:271882/251‑1 (MQ=255)                                                                                                                                                                ttGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATc                                                                       <  1:810283/251‑1 (MQ=255)                                                                                                                                                                   aTGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCcg                                                                     >  1:95593/1‑250 (MQ=255)                                                                                                                                                                           ttGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCt                                                             >  1:307316/1‑250 (MQ=255)                                                                                                                                                                             ggTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACtt                                                                                                                              >  1:492076/1‑183 (MQ=255)                                                                                                                                                                                    tcctcCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGAcc                                                    >  1:104962/4‑250 (MQ=255)                                                                                                                                                                                      ctcCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGAt                                                                                                                                                       >  1:691766/2‑149 (MQ=255)                                                                                                                                                                                        ccaccaACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAAc                                                                                                                                                  <  1:9913/152‑1 (MQ=255)                                                                                                                                                                                         caccaacgcccACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACg                                              <  1:177208/243‑1 (MQ=255)                                                                                                                                                                                         caccaaCACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACg                                              <  1:74194/247‑1 (MQ=255)                                                                                                                                                                                         caccaaCACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACg                                              <  1:31671/247‑1 (MQ=255)                                                                                                                                                                                            caaCACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCAc                                           <  1:562509/250‑1 (MQ=255)                                                                                                                                                                                               cacCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCAccgc                                        <  1:700079/251‑1 (MQ=255)                                                                                                                                                                                                 cccACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGAtt                                                                                                                                                      >  1:660464/1‑139 (MQ=255)                                                                                                                                                                                                       cgccGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGa                                 <  1:515912/248‑1 (MQ=255)                                                                                                                                                                                                          cGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATAc                             <  1:787050/251‑1 (MQ=255)                                                                                                                                                                                                          cGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATAc                             <  1:726700/251‑1 (MQ=255)                                                                                                                                                                                                             tttCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGtt                          <  1:613826/251‑1 (MQ=255)                                                                                                                                                                                                             tttCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGtt                          <  1:886349/251‑1 (MQ=255)                                                                                                                                                                                                                ccATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTaaa                       >  1:782928/1‑251 (MQ=255)                                                                                                                                                                                                                     gAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAAc                                                                                                                                <  1:573473/141‑1 (MQ=255)                                                                                                                                                                                                                     gAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGtgt                   >  1:596399/1‑248 (MQ=255)                                                                                                                                                                                                                          cgcccGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCt                                                             >  1:814144/5‑203 (MQ=255)                                                                                                                                                                                                                               gTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCcg                                                                     >  1:738331/1‑190 (MQ=255)                                                                                                                                                                                                                               gTGCCGGCAAACACATTTCCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAATCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATc                                                                       <  1:436171/188‑1 (MQ=255)                                                                                                                                                                                                                                tGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATg        <  1:565819/250‑1 (MQ=255)                                                                                                                                                                                                                                      cAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTGTCCGGCGGGATGTTCttt  <  1:303974/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           GTCGCGTGCATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCACACAATGGGCGAAAGGCATGGGCATTCCACACGTTTATGAAGACTACAAACACATTCTGCAGGATCCTGAAATTGATGCTGTGTTGGTTTGTTCTTCCACAAACACCCACGCACCGATTTCCATTGAAGCCGCTCGTGCCGGCAAACACATTTTCTGTGAAAAACCGGTGGATGCCGATGTGAATCGCATTAAAGAAGTCTTAGTCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGTGAACCGCACTTAATCCGCGTTACCTCACGCGACCCTGACGCACCGCCGATTGAATACGTTAAAGTATCCAGCGGGATGTTCTTT  >  1/1974076‑1974554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑