Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,974,275	A→G	A79A (GCA→GCG)	unknown →	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,974,275	0	A	G	100.0%	187.1 / NA	68	A79A (GCA→GCG)	unknown	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/29);  total (39/29)

GATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTGCATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCACACAATGGGCGAAAGGCATGGGCATTCCACACGTTTATGAAGACTACAAACACATTCTGCAGGATCCTGAAATTGATGCTGTGTTGGTTTGTTCTTCCACAAACACCCACGCACCGATTTCCATTGAAGCCGCTCGTGCCGGCAAACACATTTTCTGTGAAAAACCGGTGGATGCCGATGTGAATCGCATTAAAGAAGTCTTAGTCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGTGAACCGCACTTAATCCGCGTTACCTCACGCGACCCTGACGCACCGC  >  1/1974030‑1974516                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  gATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGAt                                                                                                                                                                                                                                              >  1:84756/1‑251 (MQ=255)         cATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTg                                                                                                                                                                                                                                      <  1:885502/251‑1 (MQ=255)           tGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGaa                                                                                                                                                                                                                                    >  1:578386/1‑251 (MQ=255)            gATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTcc                                                                                                                                                                                                                                          >  1:825602/1‑244 (MQ=255)                      ggTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTg                                                                                                                                                                                                                                      <  1:183363/238‑1 (MQ=255)                      ggTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTg                                                                                                                                                                                                                          >  1:223126/1‑250 (MQ=255)                      ggTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTg                                                                                                                                                                                                                          >  1:372249/1‑250 (MQ=255)                       gTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGc                                                                                                                                                                                                                         >  1:661052/1‑250 (MQ=255)                                    cGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACAttt                                                                                                                                                                                                           <  1:799236/251‑1 (MQ=255)                                     ggTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACAtttt                                                                                                                                                                                                          >  1:313609/1‑251 (MQ=255)                                      gTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACAtttt                                                                                                                                                                                                          >  1:91786/1‑250 (MQ=255)                                               gTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGaaaaa                                                                                                                                                                                                >  1:594170/1‑251 (MQ=255)                                               gTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGaaaaa                                                                                                                                                                                                >  1:162760/1‑251 (MQ=255)                                                        aTTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGAt                                                                                                                                                                                       <  1:326067/251‑1 (MQ=255)                                                               aaGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGAtg                                                                                                                                                                                <  1:700048/251‑1 (MQ=255)                                                                  cATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGAtgtaa                                                                                                                                                                             >  1:513249/1‑249 (MQ=255)                                                                    ttACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAAt                                                                                                                                                                           <  1:330953/251‑1 (MQ=255)                                                                                   aaGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTc                                                                                                                                                            <  1:866012/251‑1 (MQ=255)                                                                                          tGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAgccg                                                                                                                                                     <  1:383919/251‑4 (MQ=255)                                                                                             aaTCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTc                                                                                                                                                            >  1:356828/1‑241 (MQ=255)                                                                                                  aaGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGaa                                                                                                                                             <  1:461379/251‑1 (MQ=255)                                                                                                       aTTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGc                                                                                                                                        <  1:198963/251‑1 (MQ=255)                                                                                                                                        caATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAg                                                                                                                                         >  1:547738/1‑217 (MQ=255)                                                                                                                                        caATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACcgtcg                                                                                                       >  1:341536/1‑251 (MQ=255)                                                                                                                                        caATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACcgtcg                                                                                                       <  1:204822/251‑1 (MQ=255)                                                                                                                                                 aaaGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGAcc                                                                                               <  1:587679/250‑1 (MQ=255)                                                                                                                                                    ggCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAGTCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACaa                                                                                           >  1:441467/1‑251 (MQ=255)                                                                                                                                                    ggCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACaa                                                                                           >  1:362919/1‑251 (MQ=255)                                                                                                                                                      cATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAAc                                                                                          <  1:404763/250‑1 (MQ=255)                                                                                                                                                            cATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAg                                                                                   <  1:806970/251‑1 (MQ=255)                                                                                                                                                             aTTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAg                                                                                   >  1:93938/1‑250 (MQ=255)                                                                                                                                                              ttCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGc                                                                                  >  1:289581/1‑250 (MQ=255)                                                                                                                                                                    acGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAg                                                                           <  1:848513/251‑1 (MQ=255)                                                                                                                                                                     cGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGAcc                                                                                               >  1:67125/1‑230 (MQ=255)                                                                                                                                                                     cGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGa                                                                          >  1:666265/1‑251 (MQ=255)                                                                                                                                                                       tttATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGCTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGc                                                                                  >  1:753810/1‑241 (MQ=255)                                                                                                                                                                         tATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGAcc                                                                        <  1:348367/249‑1 (MQ=255)                                                                                                                                                                               gACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAg                                                                                   <  1:821504/232‑1 (MQ=255)                                                                                                                                                                                 ctataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGc                                                                                  >  1:784753/5‑231 (MQ=255)                                                                                                                                                                                 ctataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGAc                                                                         <  1:709802/236‑1 (MQ=255)                                                                                                                                                                                 ctataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGaaa                                                              >  1:669229/5‑251 (MQ=255)                                                                                                                                                                                 ctataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGaaa                                                              >  1:868873/5‑251 (MQ=255)                                                                                                                                                                                 ctataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGaaa                                                              >  1:63145/5‑251 (MQ=255)                                                                                                                                                                                   ataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGaaaa                                                             >  1:100089/3‑250 (MQ=255)                                                                                                                                                                                   ataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGaaaa                                                             >  1:338905/3‑250 (MQ=255)                                                                                                                                                                                              ttGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGAt                                                                                                                 >  1:512368/2‑187 (MQ=255)                                                                                                                                                                                                     aTCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGTGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAg                                                                           <  1:454166/218‑1 (MQ=255)                                                                                                                                                                                                     aTCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCg                                          >  1:762072/1‑251 (MQ=255)                                                                                                                                                                                                      tCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCg                                          >  1:109358/1‑250 (MQ=255)                                                                                                                                                                                                       ccTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGAcc                                                                        >  1:367340/1‑219 (MQ=255)                                                                                                                                                                                                       ccTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCa                                        >  1:261634/1‑251 (MQ=255)                                                                                                                                                                                                            aaTTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGAcc                                                                        <  1:364457/214‑1 (MQ=255)                                                                                                                                                                                                            aaTTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCggcga                                              <  1:200208/240‑4 (MQ=255)                                                                                                                                                                                                            aaTTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTaa                                   >  1:19409/1‑251 (MQ=255)                                                                                                                                                                                                              ttGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATc                                 <  1:271882/251‑1 (MQ=255)                                                                                                                                                                                                              ttGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATc                                 <  1:810283/251‑1 (MQ=255)                                                                                                                                                                                                                 aTGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCcg                               >  1:95593/1‑250 (MQ=255)                                                                                                                                                                                                                         ttGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCt                       >  1:307316/1‑250 (MQ=255)                                                                                                                                                                                                                           ggTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACtt                                                                                        >  1:492076/1‑183 (MQ=255)                                                                                                                                                                                                                                  tcctcCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGAcc              >  1:104962/4‑250 (MQ=255)                                                                                                                                                                                                                                    ctcCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGAt                                                                                                                 >  1:691766/2‑149 (MQ=255)                                                                                                                                                                                                                                      ccaccaACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAAc                                                                                                            <  1:9913/152‑1 (MQ=255)                                                                                                                                                                                                                                       caccaacgcccACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACg        <  1:177208/243‑1 (MQ=255)                                                                                                                                                                                                                                       caccaaCACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACg        <  1:74194/247‑1 (MQ=255)                                                                                                                                                                                                                                       caccaaCACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACg        <  1:31671/247‑1 (MQ=255)                                                                                                                                                                                                                                          caaCACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCAc     <  1:562509/250‑1 (MQ=255)                                                                                                                                                                                                                                             cacCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCCGCGTCACCTCCCGCGACCCTGACGCAccgc  <  1:700079/251‑1 (MQ=255)                                                                                                                                                                                                                                               cccACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGAtt                                                                                                                >  1:660464/1‑139 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  GATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTGCATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCACACAATGGGCGAAAGGCATGGGCATTCCACACGTTTATGAAGACTACAAACACATTCTGCAGGATCCTGAAATTGATGCTGTGTTGGTTTGTTCTTCCACAAACACCCACGCACCGATTTCCATTGAAGCCGCTCGTGCCGGCAAACACATTTTCTGTGAAAAACCGGTGGATGCCGATGTGAATCGCATTAAAGAAGTCTTAGTCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGTGAACCGCACTTAATCCGCGTTACCTCACGCGACCCTGACGCACCGC  >  1/1974030‑1974516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑