Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,974,242	T→C	A68A (GCT→GCC)	unknown →	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,974,242	0	T	C	100.0%	201.0 / NA	68	A68A (GCT→GCC)	unknown	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/28);  total (40/28)

GCCGTAAATACATTAAAGAAACAGCGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTGCATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCACACAATGGGCGAAAGGCATGGGCATTCCACACGTTTATGAAGACTACAAACACATTCTGCAGGATCCTGAAATTGATGCTGTGTTGGTTTGTTCTTCCACAAACACCCACGCACCGATTTCCATTGAAGCCGCTCGTGCCGGCAAACACATTTTCTGTGAAAAACCGGTGGATGCCGATGTGAATCGCATTAAAGAAGTCTTAGTCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGTGAACCGCACTTAATCCG  >  1/1974002‑1974487                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      gCCGTAAATACATTAAAGAAACAGCGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGttt                                                                                                                                                                                                                                             >  1:151793/1‑251 (MQ=255)  ccGTAAATACATTAAAGAAACAGTGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTGCAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAGCAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTg                                                                                                                                                                                                                                            <  1:130115/251‑1 (MQ=255)        aTACATTAAAGAAACAGTGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGACATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGttcct                                                                                                                                                                                                                                       >  1:434306/1‑248 (MQ=255)           cATTAAAGAAACAGTGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCca                                                                                                                                                                                                                                    >  1:91914/1‑250 (MQ=255)                   aaaCAGTGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACAcc                                                                                                                                                                                                                            >  1:122438/1‑250 (MQ=255)                         tggggATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACg                                                                                                                                                                                                                        <  1:830515/247‑1 (MQ=255)                         tggggATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGcgc                                                                                                                                                                                                                     <  1:782099/250‑3 (MQ=255)                         tggggATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGcgc                                                                                                                                                                                                                     <  1:623594/250‑3 (MQ=255)                            ggATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGttc                                                                                                                                                                                                                                         >  1:588439/1‑228 (MQ=255)                             gATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGAt                                                                                                                                                                                                                 >  1:84756/1‑251 (MQ=255)                                     cATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTg                                                                                                                                                                                                         <  1:885502/251‑1 (MQ=255)                                       tGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGaa                                                                                                                                                                                                       >  1:578386/1‑251 (MQ=255)                                        gATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATGAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGtt                                                                                                                                                                                                                                              >  1:433057/1‑211 (MQ=255)                                        gATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTcc                                                                                                                                                                                                             >  1:825602/1‑244 (MQ=255)                                                 aGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAGACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGttc                                                                                                                                                                                                                                         <  1:373150/207‑1 (MQ=255)                                                  ggTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTg                                                                                                                                                                                                         <  1:183363/238‑1 (MQ=255)                                                  ggTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTg                                                                                                                                                                                             >  1:223126/1‑250 (MQ=255)                                                  ggTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTg                                                                                                                                                                                             >  1:372249/1‑250 (MQ=255)                                                   gTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGc                                                                                                                                                                                            >  1:661052/1‑250 (MQ=255)                                                                cGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACAttt                                                                                                                                                                              <  1:799236/251‑1 (MQ=255)                                                                 ggTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACAtttt                                                                                                                                                                             >  1:313609/1‑251 (MQ=255)                                                                  gTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACAtttt                                                                                                                                                                             >  1:91786/1‑250 (MQ=255)                                                                           gTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGaaaaa                                                                                                                                                                   >  1:162760/1‑251 (MQ=255)                                                                           gTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGaaaaa                                                                                                                                                                   >  1:594170/1‑251 (MQ=255)                                                                                    aTTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGAt                                                                                                                                                          <  1:326067/251‑1 (MQ=255)                                                                                           aaGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGAtg                                                                                                                                                   <  1:700048/251‑1 (MQ=255)                                                                                              cATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGAtgtaa                                                                                                                                                >  1:513249/1‑249 (MQ=255)                                                                                                ttACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAAt                                                                                                                                              <  1:330953/251‑1 (MQ=255)                                                                                                               aaGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTc                                                                                                                               <  1:866012/251‑1 (MQ=255)                                                                                                                      tGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAgccg                                                                                                                        <  1:383919/251‑4 (MQ=255)                                                                                                                         aaTCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTc                                                                                                                               >  1:356828/1‑241 (MQ=255)                                                                                                                              aaGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGaa                                                                                                                <  1:461379/251‑1 (MQ=255)                                                                                                                                   aTTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGc                                                                                                           <  1:198963/251‑1 (MQ=255)                                                                                                                                                                    caATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAg                                                                                                            >  1:547738/1‑217 (MQ=255)                                                                                                                                                                    caATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACcgtcg                                                                          >  1:341536/1‑251 (MQ=255)                                                                                                                                                                    caATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACcgtcg                                                                          <  1:204822/251‑1 (MQ=255)                                                                                                                                                                             aaaGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGAcc                                                                  <  1:587679/250‑1 (MQ=255)                                                                                                                                                                                ggCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAGTCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACaa                                                              >  1:441467/1‑251 (MQ=255)                                                                                                                                                                                ggCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACaa                                                              >  1:362919/1‑251 (MQ=255)                                                                                                                                                                                  cATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAAc                                                             <  1:404763/250‑1 (MQ=255)                                                                                                                                                                                        cATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAg                                                      <  1:806970/251‑1 (MQ=255)                                                                                                                                                                                         aTTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAg                                                      >  1:93938/1‑250 (MQ=255)                                                                                                                                                                                          ttCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGc                                                     >  1:289581/1‑250 (MQ=255)                                                                                                                                                                                                acGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAg                                              <  1:848513/251‑1 (MQ=255)                                                                                                                                                                                                 cGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGAcc                                                                  >  1:67125/1‑230 (MQ=255)                                                                                                                                                                                                 cGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGa                                             >  1:666265/1‑251 (MQ=255)                                                                                                                                                                                                   tttATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGCTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGc                                                     >  1:753810/1‑241 (MQ=255)                                                                                                                                                                                                     tATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGAcc                                           <  1:348367/249‑1 (MQ=255)                                                                                                                                                                                                           gACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAg                                                      <  1:821504/232‑1 (MQ=255)                                                                                                                                                                                                             ctataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGc                                                     >  1:784753/5‑231 (MQ=255)                                                                                                                                                                                                             ctataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGAc                                            <  1:709802/236‑1 (MQ=255)                                                                                                                                                                                                             ctataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGaaa                                 >  1:669229/5‑251 (MQ=255)                                                                                                                                                                                                             ctataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGaaa                                 >  1:63145/5‑251 (MQ=255)                                                                                                                                                                                                             ctataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGaaa                                 >  1:868873/5‑251 (MQ=255)                                                                                                                                                                                                               ataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGaaaa                                >  1:338905/3‑250 (MQ=255)                                                                                                                                                                                                               ataaaCACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGaaaa                                >  1:100089/3‑250 (MQ=255)                                                                                                                                                                                                                          ttGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGAt                                                                                    >  1:512368/2‑187 (MQ=255)                                                                                                                                                                                                                                 aTCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGTGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAg                                              <  1:454166/218‑1 (MQ=255)                                                                                                                                                                                                                                 aTCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCg             >  1:762072/1‑251 (MQ=255)                                                                                                                                                                                                                                  tCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCg             >  1:109358/1‑250 (MQ=255)                                                                                                                                                                                                                                   ccTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGAcc                                           >  1:367340/1‑219 (MQ=255)                                                                                                                                                                                                                                   ccTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCa           >  1:261634/1‑251 (MQ=255)                                                                                                                                                                                                                                        aaTTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGAcc                                           <  1:364457/214‑1 (MQ=255)                                                                                                                                                                                                                                        aaTTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCggcga                 <  1:200208/240‑4 (MQ=255)                                                                                                                                                                                                                                        aaTTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTaa      >  1:19409/1‑251 (MQ=255)                                                                                                                                                                                                                                          ttGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATc    <  1:810283/251‑1 (MQ=255)                                                                                                                                                                                                                                          ttGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATc    <  1:271882/251‑1 (MQ=255)                                                                                                                                                                                                                                             aTGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGCGAACCGCACTTAATCcg  >  1:95593/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      GCCGTAAATACATTAAAGAAACAGCGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTGCATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCACACAATGGGCGAAAGGCATGGGCATTCCACACGTTTATGAAGACTACAAACACATTCTGCAGGATCCTGAAATTGATGCTGTGTTGGTTTGTTCTTCCACAAACACCCACGCACCGATTTCCATTGAAGCCGCTCGTGCCGGCAAACACATTTTCTGTGAAAAACCGGTGGATGCCGATGTGAATCGCATTAAAGAAGTCTTAGTCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACCCGCGTGGAAAACGGCGACATCGGTGAACCGCACTTAATCCG  >  1/1974002‑1974487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑