Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,974,209	C→T	Y57Y (TAC→TAT)	unknown →	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,974,209	0	C	T	100.0%	184.5 / NA	63	Y57Y (TAC→TAT)	unknown	Myo‑inositol 2‑dehydrogenase 1 (EC 1.1.1.18)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/32);  total (31/32)

TCCGGCAATCGCCAACCCATTTGAATATGCCGTCAAAGGCCGTAAATACATTAAAGAAACAGCGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTGCATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCACACAATGGGCGAAAGGCATGGGCATTCCACACGTTTATGAAGACTACAAACACATTCTGCAGGATCCTGAAATTGATGCTGTGTTGGTTTGTTCTTCCACAAACACCCACGCACCGATTTCCATTGAAGCCGCTCGTGCCGGCAAACACATTTTCTGTGAAAAACCGGTGGATGCCGATGTGAATCGCATTAAAGAAGTCTTAGTCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACC  >  1/1973964‑1974446                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              tCCGGCAATCGCCAACCCATTTGAATATGCCGTCAAAGGCCGTAAATACATTAAAGAAACAGTGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAaca                                                                                                                                                                                                                                          <  1:529526/251‑1 (MQ=255)  ccGGCAATCGCCAACCCATTTGAATATGCCGTCAAAGGCCGTAAATACATTAAAGAAACAGTGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAacac                                                                                                                                                                                                                                         <  1:760217/251‑1 (MQ=255)  ccGGCAATCGCCAACCCATTTGAATATGCCGTCAAAGGCCGTAAATACATTAAAGAAACAGTGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAacac                                                                                                                                                                                                                                         <  1:720781/251‑1 (MQ=255)  ccGGCAATCGCCAACCCATTTGAATATGCCGTCAAAGGCCGTAAATACATTAAAGAAACAGTGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAacac                                                                                                                                                                                                                                         <  1:489647/251‑1 (MQ=255)                  cATTTGAATATGCCGTCAAAGGCCGTAAATACATTAAAGAAACAGTGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAaca                                                                                                                                                                                                                                          <  1:774695/234‑1 (MQ=255)                  cATTTGAATATGCCGTCAAAGGCCGTAAATACATTAAAGAAACAGTGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATc                                                                                                                                                                                                                            >  1:142155/1‑248 (MQ=255)                             gCCGTCAAAGGCCGTAAATACATTAAAGAAACAGTGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATaaa                                                                                                                                                                                                                                            <  1:230793/221‑1 (MQ=255)                             gCCGTCAAAGGCCGTAAATACATTAAAGAAACAGTGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAAt                                                                                                                                                                                                                     >  1:799284/1‑244 (MQ=255)                                       gCCGTAAATACATTAAAGAAACAGCGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGttt                                                                                                                                                                                                    >  1:151793/1‑251 (MQ=255)                                        ccGTAAATACATTAAAGAAACAGTGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTGCAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAGCAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTg                                                                                                                                                                                                   <  1:130115/251‑1 (MQ=255)                                              aTACATTAAAGAAACAGTGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGACATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGttcct                                                                                                                                                                                              >  1:434306/1‑248 (MQ=255)                                                 cATTAAAGAAACAGTGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCca                                                                                                                                                                                           >  1:91914/1‑250 (MQ=255)                                                         aaaCAGTGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACAcc                                                                                                                                                                                   >  1:122438/1‑250 (MQ=255)                                                               tggggATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACg                                                                                                                                                                               <  1:830515/247‑1 (MQ=255)                                                               tggggATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGcgc                                                                                                                                                                            <  1:623594/250‑3 (MQ=255)                                                               tggggATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGcgc                                                                                                                                                                            <  1:782099/250‑3 (MQ=255)                                                                  ggATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGttc                                                                                                                                                                                                >  1:588439/1‑228 (MQ=255)                                                                   gATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGAt                                                                                                                                                                        >  1:84756/1‑251 (MQ=255)                                                                           cATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTg                                                                                                                                                                <  1:885502/251‑1 (MQ=255)                                                                             tGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGaa                                                                                                                                                              >  1:578386/1‑251 (MQ=255)                                                                              gATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATGAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGtt                                                                                                                                                                                                     >  1:433057/1‑211 (MQ=255)                                                                              gATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAac                                                                                                                                                                                                                                           >  1:193276/1‑173 (MQ=255)                                                                              gATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTcc                                                                                                                                                                    >  1:825602/1‑244 (MQ=255)                                                                                   aaGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGc                                                                                                                                                                                                               <  1:301159/196‑1 (MQ=255)                                                                                       aGGTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAGACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGttc                                                                                                                                                                                                <  1:373150/207‑1 (MQ=255)                                                                                        ggTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTg                                                                                                                                                                <  1:183363/238‑1 (MQ=255)                                                                                        ggTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTg                                                                                                                                                    >  1:372249/1‑250 (MQ=255)                                                                                        ggTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTg                                                                                                                                                    >  1:223126/1‑250 (MQ=255)                                                                                         gTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAaca                                                                                                                                                                                                                                          <  1:692033/163‑1 (MQ=255)                                                                                         gTATTATCGGCGCCGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGc                                                                                                                                                   >  1:661052/1‑250 (MQ=255)                                                                                                      cGGTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACAttt                                                                                                                                     <  1:799236/251‑1 (MQ=255)                                                                                                       ggTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACAtttt                                                                                                                                    >  1:313609/1‑251 (MQ=255)                                                                                                        gTCGTATCGGTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACAtttt                                                                                                                                    >  1:91786/1‑250 (MQ=255)                                                                                                                 gTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGaaaaa                                                                                                                          >  1:162760/1‑251 (MQ=255)                                                                                                                 gTCGCGTACATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGaaaaa                                                                                                                          >  1:594170/1‑251 (MQ=255)                                                                                                                          aTTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGAt                                                                                                                 <  1:326067/251‑1 (MQ=255)                                                                                                                                 aaGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGAtg                                                                                                          <  1:700048/251‑1 (MQ=255)                                                                                                                                    cATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGAtgtaa                                                                                                       >  1:513249/1‑249 (MQ=255)                                                                                                                                      ttACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAaca                                                                                                                                                                                                                                          <  1:289971/118‑1 (MQ=255)                                                                                                                                      ttACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAAt                                                                                                     <  1:330953/251‑1 (MQ=255)                                                                                                                                        aCAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAac                                                                                                                                                                                                                                           <  1:136855/115‑1 (MQ=255)                                                                                                                                           aaaTATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAAtt                                                                                                                                                                                                                    >  1:339324/1‑135 (MQ=255)                                                                                                                                                     aaGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTc                                                                                      <  1:866012/251‑1 (MQ=255)                                                                                                                                                            tGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAgccg                                                                               <  1:383919/251‑4 (MQ=255)                                                                                                                                                               aaTCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTc                                                                                      >  1:356828/1‑241 (MQ=255)                                                                                                                                                                    aaGCGATTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGaa                                                                       <  1:461379/251‑1 (MQ=255)                                                                                                                                                                         aTTTCCGATGTTCGCGTCACCGATGAGCTCAAACAATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGc                                                                  <  1:198963/251‑1 (MQ=255)                                                                                                                                                                                                          caATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAg                                                                   >  1:547738/1‑217 (MQ=255)                                                                                                                                                                                                          caATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACcgtcg                                 >  1:341536/1‑251 (MQ=255)                                                                                                                                                                                                          caATGGGCGAAAGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACcgtcg                                 <  1:204822/251‑1 (MQ=255)                                                                                                                                                                                                                   aaaGGCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGAcc                         <  1:587679/250‑1 (MQ=255)                                                                                                                                                                                                                      ggCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAGTCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACaa                     >  1:441467/1‑251 (MQ=255)                                                                                                                                                                                                                      ggCATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACaa                     >  1:362919/1‑251 (MQ=255)                                                                                                                                                                                                                        cATGGGCATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAAc                    <  1:404763/250‑1 (MQ=255)                                                                                                                                                                                                                              cATTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAg             <  1:806970/251‑1 (MQ=255)                                                                                                                                                                                                                               aTTCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAg             >  1:93938/1‑250 (MQ=255)                                                                                                                                                                                                                                ttCCGCACGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGc            >  1:289581/1‑250 (MQ=255)                                                                                                                                                                                                                                      acGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAg     <  1:848513/251‑1 (MQ=255)                                                                                                                                                                                                                                       cGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGAcc                         >  1:67125/1‑230 (MQ=255)                                                                                                                                                                                                                                       cGTTTATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGa    >  1:666265/1‑251 (MQ=255)                                                                                                                                                                                                                                         tttATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGCTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGc            >  1:753810/1‑241 (MQ=255)                                                                                                                                                                                                                                           tATGAAGACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGAcc  <  1:348367/249‑1 (MQ=255)                                                                                                                                                                                                                                                 gACTATAAACACATTTTGCAGGATCCTGAAATTGATGCCGTGTTGGTTTGTTCCTCCACCAACACCCACGCGCCGATTTCCATTGAAGCCGCCCGTGCCGGCAAACACATTTTCTGCGAAAAACCGGTGGATGCCGATGTAAATCGCATTAAAGAAGTCTTAGCCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAg             <  1:821504/232‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              TCCGGCAATCGCCAACCCATTTGAATATGCCGTCAAAGGCCGTAAATACATTAAAGAAACAGCGGGGATTTAATCATGATTAAAGTAGGTATTATCGGCGCCGGTCGTATCGGTCGCGTGCATTCCGAAAGCATTACAAAATATGTCAAAGGCGCTGAAATCAAAGCGATTTCCGATGTTCGCGTCACCGATGAGCTCACACAATGGGCGAAAGGCATGGGCATTCCACACGTTTATGAAGACTACAAACACATTCTGCAGGATCCTGAAATTGATGCTGTGTTGGTTTGTTCTTCCACAAACACCCACGCACCGATTTCCATTGAAGCCGCTCGTGCCGGCAAACACATTTTCTGTGAAAAACCGGTGGATGCCGATGTGAATCGCATTAAAGAAGTCTTAGTCGAAGTGGAAAAAGCGGGCGTGAAATTCCAGGTAGGATTCAACCGTCGTTTTGACCACAACTTTAAAGCCATCAAGACC  >  1/1973964‑1974446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑