Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,972,998	T→C	V620V (GTT→GTC)	unknown →	3D‑(3,5/4)‑trihydroxycyclohexane‑1,2‑dione hydrolase (EC 3.7.1.22)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,972,998	0	T	C	100.0%	227.3 / NA	79	V620V (GTT→GTC)	unknown	3D‑(3,5/4)‑trihydroxycyclohexane‑1,2‑dione hydrolase (EC 3.7.1.22)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/42);  total (37/42)

CTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGTAAAACCTATAAAGTCACCACTGAAGAGGAATTACGCGCCGCCCTTGCCGATGCGAAAAAACAAACCGTCTCTACGTTGATTGACATAAAAGTCTTGCCAAAAACCATGATTCACGGCTACGGCAGCTGGTGGCACGTTGGCGTAGCAGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCATAAAAGCAGCGTGAAACACATTAACGAAGCAAGACGCTATTAATCCG‑‑ATTCAATTTATTATCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACGATGATATGCCTGATCTTGGCAAAGAGAATACCTTTGAAC  >  1/1972761‑1973230                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           cTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGtgccggaag                                                                                                                                                                                                                               <  1:206278/251‑9 (MQ=255)                   cTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGaaaaaa                                                                                                                                                                                                             <  1:805843/251‑1 (MQ=255)                     tCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAAt                                                                                                                                                                                                            >  1:131724/1‑250 (MQ=255)                     tCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAAt                                                                                                                                                                                                            >  1:29550/1‑250 (MQ=255)                                    ccAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACaaa                                                                                                                                                                                            >  1:502984/1‑251 (MQ=255)                                    ccAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACaaa                                                                                                                                                                                            <  1:806101/251‑1 (MQ=255)                                      aaTAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACaaaaa                                                                                                                                                                                          >  1:404485/1‑250 (MQ=255)                                       aTAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCATCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACaaaaac                                                                                                                                                                                         <  1:456292/251‑3 (MQ=255)                                         aaaTTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAaacag                                                                                                                                                                                       >  1:510202/1‑251 (MQ=255)                                         aaaTTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAaacag                                                                                                                                                                                       <  1:761364/251‑1 (MQ=255)                                           aTTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCg                                                                                                                                                                                     <  1:517368/251‑1 (MQ=255)                                           aTTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCg                                                                                                                                                                                     >  1:26199/1‑251 (MQ=255)                                                   gcggATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAaca                                                                                                                                                                              >  1:433129/1‑250 (MQ=255)                                                       aTTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACAtt                                                                                                                                                                          <  1:742173/250‑1 (MQ=255)                                                         tCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAAc                                                                                                                                                                       <  1:522534/251‑1 (MQ=255)                                                         tCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAAc                                                                                                                                                                       <  1:497589/251‑1 (MQ=255)                                                            tGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAacac                                                                                                                                                                             <  1:145380/242‑1 (MQ=255)                                                            tGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGa                                                                                                                                                                     <  1:707976/250‑1 (MQ=255)                                                            tGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGa                                                                                                                                                                     <  1:208526/250‑1 (MQ=255)                                                             gCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAGAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAg                                                                                                                                                                   >  1:841229/1‑251 (MQ=255)                                                                cGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAGCACATTAACGAAGCaa                                                                                                                                                                >  1:857660/1‑251 (MQ=255)                                                                          gCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGttatta                                                                                                                                                      >  1:293476/1‑251 (MQ=255)                                                                               gAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCg                                                                                                                                                 >  1:496014/1‑251 (MQ=255)                                                                                 aTGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAacac                                                                                                                                                                             >  1:134178/1‑221 (MQ=255)                                                                                       cATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCgtcatttaa                                                                                                                                         <  1:562133/251‑9 (MQ=255)                                                                                        aTCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGGCATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAAt                                                                                                                                                    >  1:165400/1‑239 (MQ=255)                                                                                        aTCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATc                                                                                                                                                   <  1:241425/240‑1 (MQ=255)                                                                                         tCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCgtcatttaatt                                                                                                                                       >  1:722259/1‑241 (MQ=255)                                                                                            tACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCgtcatttaatttat                                                                                                                                    <  1:93101/251‑14 (MQ=255)                                                                                                 ttgcaaaaCCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATttatttct                                                                                                                                >  1:750369/5‑247 (MQ=255)                                                                                                         ccTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACaaa                                                                                                                                                                                            >  1:163169/1‑182 (MQ=255)                                                                                                         ccTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTaa                                                                                                                                                     >  1:458110/1‑221 (MQ=255)                                                                                                         ccTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTaa                                                                                                                      >  1:27392/1‑251 (MQ=255)                                                                                                                 gTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAAcc                                                                                                                    >  1:261664/1‑245 (MQ=255)                                                                                                                 gTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCAct                                                                                                                 >  1:807602/1‑248 (MQ=255)                                                                                                                     ccacCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAAcc                                                                                                                    >  1:76704/1‑241 (MQ=255)                                                                                                                       accgAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTgaga                                                                                                        <  1:891012/248‑1 (MQ=255)                                                                                                                         cgAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAg                                                                                                      <  1:712189/250‑1 (MQ=255)                                                                                                                             gagGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAAtt                                                                                                                         >  1:485852/1‑228 (MQ=255)                                                                                                                               ggAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTg                                                                                                           <  1:375328/240‑1 (MQ=255)                                                                                                                                gAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCgtcatttaattt                                                                                                                                      >  1:787541/1‑202 (MQ=255)                                                                                                                                gAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAac                                                                                                <  1:812521/250‑1 (MQ=255)                                                                                                                                 aaTTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAacac                                                                                              <  1:182787/251‑1 (MQ=255)                                                                                                                                      cgcgCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGa                                                                                         <  1:584368/251‑1 (MQ=255)                                                                                                                                            gccCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGaa                                                                                        >  1:683153/1‑246 (MQ=255)                                                                                                                                                   ccGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACg                                                                             <  1:308149/250‑1 (MQ=255)                                                                                                                                                             aaaaCAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGt                                                                  <  1:739873/251‑1 (MQ=255)                                                                                                                                                                 caaacCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCg                                                                                                                                                                                                                                        >  1:657219/1‑82 (MQ=255)                                                                                                                                                                        tctcTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGa                                                                                                                                                                     >  1:380854/1‑142 (MQ=255)                                                                                                                                                                        tctcTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAg                                                                                                      >  1:29164/1‑204 (MQ=255)                                                                                                                                                                        tctcTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAAt                                                       >  1:364495/1‑251 (MQ=255)                                                                                                                                                                        tctcTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGACATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTgaga                                                                                                        >  1:477665/1‑202 (MQ=255)                                                                                                                                                                         ctctACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTg                                                                                                           <  1:876059/198‑1 (MQ=255)                                                                                                                                                                                ctgattgaCATAAAAGTCTTGTCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGa                                               >  1:800439/2‑251 (MQ=255)                                                                                                                                                                                 tgattgaCATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGAc                                              <  1:144527/251‑1 (MQ=255)                                                                                                                                                                                       aCATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAg                                                                                                                                                               <  1:43667/133‑1 (MQ=255)                                                                                                                                                                                       aCATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATc                                                                                                                                                   >  1:323678/1‑145 (MQ=255)                                                                                                                                                                                       aCATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACga                                        <  1:152660/251‑1 (MQ=255)                                                                                                                                                                                           aaaaGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACgatgat                                    <  1:136228/251‑1 (MQ=255)                                                                                                                                                                                           aaaaGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACgatgat                                    <  1:878330/251‑1 (MQ=255)                                                                                                                                                                                           aaaaGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACgatgat                                    <  1:256080/251‑1 (MQ=255)                                                                                                                                                                                            aaaGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACGATGata                                   <  1:319500/251‑1 (MQ=255)                                                                                                                                                                                             aaGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACGATGatat                                  <  1:634394/251‑1 (MQ=255)                                                                                                                                                                                               gTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCgtcatttaattt                                                                                                                                      >  1:675122/1‑139 (MQ=255)                                                                                                                                                                                                   tGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAA‑‑ACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACGATGATATGCCTGATc                          <  1:644104/251‑1 (MQ=255)                                                                                                                                                                                                    gccgaaaaCCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTg                                                                                                           >  1:65476/5‑171 (MQ=255)                                                                                                                                                                                                     ccgaaaaCCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACGATGATATGCCTGATc                          <  1:186878/248‑1 (MQ=255)                                                                                                                                                                                                     ccgaaaaCCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACGATGATATGCCTGATc                          <  1:590094/248‑1 (MQ=255)                                                                                                                                                                                                           aCCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAg                                                                                                      >  1:858825/1‑169 (MQ=255)                                                                                                                                                                                                            ccATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTAt                                                                >  1:177016/1‑206 (MQ=255)                                                                                                                                                                                                            ccATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACGATGATATGCCTGATCTTGGCaa                   >  1:95107/1‑251 (MQ=255)                                                                                                                                                                                                                 atccACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACga                                        <  1:371361/222‑1 (MQ=255)                                                                                                                                                                                                                  tccACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACGATGATATGCCTGATCTTGGCAAAGAGaa             >  1:769742/3‑251 (MQ=255)                                                                                                                                                                                                                      cGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGc                                                                                     <  1:585895/175‑1 (MQ=255)                                                                                                                                                                                                                      cGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGt                                                                  <  1:835324/194‑1 (MQ=255)                                                                                                                                                                                                                         cTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACGATGATATGCCTGATCTTGGCAAAGAGAATACCtt       <  1:719622/250‑1 (MQ=255)                                                                                                                                                                                                                              gCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACGATGATATGCCTGATCTTGGCAAAGAGAATACCTTTGAAc  <  1:770306/250‑1 (MQ=255)                                                                                                                                                                                                                              gCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACGATGATATGCCTGATCTTGGCAAAGAGAATACCTTTGAAc  <  1:758827/250‑1 (MQ=255)                                                                                                                                                                                                                              gCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACGATGATATGCCTGATCTTGGCAAAGAGAATACCTCTGAAc  <  1:690253/250‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                           CTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGTAAAACCTATAAAGTCACCACTGAAGAGGAATTACGCGCCGCCCTTGCCGATGCGAAAAAACAAACCGTCTCTACGTTGATTGACATAAAAGTCTTGCCAAAAACCATGATTCACGGCTACGGCAGCTGGTGGCACGTTGGCGTAGCAGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCATAAAAGCAGCGTGAAACACATTAACGAAGCAAGACGCTATTAATCCG‑‑ATTCAATTTATTATCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAATCGGTTGGACTAACGATGATATGCCTGATCTTGGCAAAGAGAATACCTTTGAAC  >  1/1972761‑1973230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑