Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,972,936	T→C	L600L (TTG→CTG)	unknown →	3D‑(3,5/4)‑trihydroxycyclohexane‑1,2‑dione hydrolase (EC 3.7.1.22)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,972,936	0	T	C	100.0%	222.5 / NA	74	L600L (TTG→CTG)	unknown	3D‑(3,5/4)‑trihydroxycyclohexane‑1,2‑dione hydrolase (EC 3.7.1.22)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/33);  total (41/33)

ACGTAATTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGTAAAACCTATAAAGTCACCACTGAAGAGGAATTACGCGCCGCCCTTGCCGATGCGAAAAAACAAACCGTCTCTACGTTGATTGACATAAAAGTCTTGCCAAAAACCATGATTCACGGCTACGGCAGCTGGTGGCACGTTGGCGTAGCAGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCATAAAAGCAGCGTGAAACACATTAACGAAGCAAGACGCTATTAATCCG‑‑ATTCAATTTATTATCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAAT  >  1/1972691‑1973177                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    aCGTAATTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGctgatt                                                                                                                                                                                                                                                >  1:53009/1‑251 (MQ=255)  cGTAATTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGctgattg                                                                                                                                                                                                                                               <  1:576182/251‑1 (MQ=255)      aTTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACAt                                                                                                                                                                                                                                           >  1:855528/1‑251 (MQ=255)      aTTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACAt                                                                                                                                                                                                                                           <  1:854787/251‑1 (MQ=255)      aTTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACAt                                                                                                                                                                                                                                           >  1:617945/1‑251 (MQ=255)           aTTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAg                                                                                                                                                                                                                                      <  1:68296/251‑1 (MQ=255)            ttCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGt                                                                                                                                                                                                                                     >  1:489500/1‑251 (MQ=255)                        aCAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAAc                                                                                                                                                                                                                         <  1:118791/251‑1 (MQ=255)                                  gTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGAtcca                                                                                                                                                                                                                >  1:793109/1‑247 (MQ=255)                                     ttAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGc                                                                                                                                                                                                            <  1:468516/251‑1 (MQ=255)                                      tAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCt                                                                                                                                                                                                           <  1:689276/251‑1 (MQ=255)                                          aaTCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACgg                                                                                                                                                                                                       <  1:403900/251‑1 (MQ=255)                                                        cAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCAc                                                                                                                                                                                          <  1:570390/250‑1 (MQ=255)                                                           tGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGtcg                                                                                                                                                                                      >  1:36858/1‑249 (MQ=255)                                                            ggTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGGATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGc                                                                                                                                                                                                            <  1:100384/228‑1 (MQ=255)                                                            ggTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAAc                                                                                                                                                                                                                         >  1:33176/1‑215 (MQ=255)                                                            ggTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGtcgg                                                                                                                                                                                     >  1:378984/1‑248 (MQ=255)                                                            ggTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGtcgg                                                                                                                                                                                     >  1:818479/1‑248 (MQ=255)                                                            ggTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGtcgg                                                                                                                                                                                     >  1:538095/1‑248 (MQ=255)                                                            ggTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGCGGCACGtcgg                                                                                                                                                                                     >  1:155157/1‑248 (MQ=255)                                                            ggTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATCGACATAAAAGTCTTGCCGAAAACCATGATCCACGGc                                                                                                                                                                                                            >  1:873282/1‑228 (MQ=255)                                                                       cTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGtgccggaag                                                                                                                                                                          <  1:206278/251‑9 (MQ=255)                                                                                         cTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGaaaaaa                                                                                                                                                        <  1:805843/251‑1 (MQ=255)                                                                                           tCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAAcc                                                                                                                                                                                                                        <  1:585812/185‑1 (MQ=255)                                                                                           tCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAAt                                                                                                                                                       >  1:29550/1‑250 (MQ=255)                                                                                           tCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAAt                                                                                                                                                       >  1:131724/1‑250 (MQ=255)                                                                                                          ccAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACaaa                                                                                                                                       <  1:806101/251‑1 (MQ=255)                                                                                                          ccAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACaaa                                                                                                                                       >  1:502984/1‑251 (MQ=255)                                                                                                            aaTAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACaaaaa                                                                                                                                     >  1:404485/1‑250 (MQ=255)                                                                                                             aTAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCATCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACaaaaac                                                                                                                                    <  1:456292/251‑3 (MQ=255)                                                                                                               aaaTTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAaacag                                                                                                                                  <  1:761364/251‑1 (MQ=255)                                                                                                               aaaTTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAaacag                                                                                                                                  >  1:510202/1‑251 (MQ=255)                                                                                                                 aTTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCg                                                                                                                                >  1:26199/1‑251 (MQ=255)                                                                                                                 aTTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCg                                                                                                                                <  1:517368/251‑1 (MQ=255)                                                                                                                         gcggATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAaca                                                                                                                         >  1:433129/1‑250 (MQ=255)                                                                                                                             aTTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACAtt                                                                                                                     <  1:742173/250‑1 (MQ=255)                                                                                                                              ttCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGAtc                                                                                                                                                                                                                  >  1:32937/1‑155 (MQ=255)                                                                                                                               tCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAAc                                                                                                                  <  1:497589/251‑1 (MQ=255)                                                                                                                               tCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAAc                                                                                                                  <  1:522534/251‑1 (MQ=255)                                                                                                                                  tGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAacac                                                                                                                        <  1:145380/242‑1 (MQ=255)                                                                                                                                  tGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGa                                                                                                                <  1:707976/250‑1 (MQ=255)                                                                                                                                  tGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGa                                                                                                                <  1:208526/250‑1 (MQ=255)                                                                                                                                   gCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAGAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAg                                                                                                              >  1:841229/1‑251 (MQ=255)                                                                                                                                      cGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAGCACATTAACGAAGCaa                                                                                                           >  1:857660/1‑251 (MQ=255)                                                                                                                                                gCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGttatta                                                                                                 >  1:293476/1‑251 (MQ=255)                                                                                                                                                     gAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCg                                                                                            >  1:496014/1‑251 (MQ=255)                                                                                                                                                       aTGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAacac                                                                                                                        >  1:134178/1‑221 (MQ=255)                                                                                                                                                             cATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCgtcatttaa                                                                                    <  1:562133/251‑9 (MQ=255)                                                                                                                                                              aTCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGGCATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAAt                                                                                               >  1:165400/1‑239 (MQ=255)                                                                                                                                                              aTCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATc                                                                                              <  1:241425/240‑1 (MQ=255)                                                                                                                                                               tCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCgtcatttaatt                                                                                  >  1:722259/1‑241 (MQ=255)                                                                                                                                                                  tACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCgtcatttaatttat                                                                               <  1:93101/251‑14 (MQ=255)                                                                                                                                                                       ttgcaaaaCCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATttatttct                                                                           >  1:750369/5‑247 (MQ=255)                                                                                                                                                                               ccTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACaaa                                                                                                                                       >  1:163169/1‑182 (MQ=255)                                                                                                                                                                               ccTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTaa                                                                                                >  1:458110/1‑221 (MQ=255)                                                                                                                                                                               ccTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTaa                                                                 >  1:27392/1‑251 (MQ=255)                                                                                                                                                                                       gTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAAcc                                                               >  1:261664/1‑245 (MQ=255)                                                                                                                                                                                       gTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCAct                                                            >  1:807602/1‑248 (MQ=255)                                                                                                                                                                                           ccacCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAAcc                                                               >  1:76704/1‑241 (MQ=255)                                                                                                                                                                                             accgAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTgaga                                                   <  1:891012/248‑1 (MQ=255)                                                                                                                                                                                               cgAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAg                                                 <  1:712189/250‑1 (MQ=255)                                                                                                                                                                                                   gagGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAAtt                                                                    >  1:485852/1‑228 (MQ=255)                                                                                                                                                                                                     ggAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTg                                                      <  1:375328/240‑1 (MQ=255)                                                                                                                                                                                                      gAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCgtcatttaattt                                                                                 >  1:787541/1‑202 (MQ=255)                                                                                                                                                                                                      gAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAac                                           <  1:812521/250‑1 (MQ=255)                                                                                                                                                                                                       aaTTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAacac                                         <  1:182787/251‑1 (MQ=255)                                                                                                                                                                                                            cgcgCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGa                                    <  1:584368/251‑1 (MQ=255)                                                                                                                                                                                                                  gccCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGaa                                   >  1:683153/1‑246 (MQ=255)                                                                                                                                                                                                                         ccGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACg                        <  1:308149/250‑1 (MQ=255)                                                                                                                                                                                                                                   aaaaCAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGt             <  1:739873/251‑1 (MQ=255)                                                                                                                                                                                                                                       caaacCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCg                                                                                                                                                                                   >  1:657219/1‑82 (MQ=255)                                                                                                                                                                                                                                              tctcTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGa                                                                                                                >  1:380854/1‑142 (MQ=255)                                                                                                                                                                                                                                              tctcTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAg                                                 >  1:29164/1‑204 (MQ=255)                                                                                                                                                                                                                                              tctcTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAAt  >  1:364495/1‑251 (MQ=255)                                                                                                                                                                                                                                              tctcTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGACATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTgaga                                                   >  1:477665/1‑202 (MQ=255)                                                                                                                                                                                                                                               ctctACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCACTCTGTTg                                                      <  1:876059/198‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ACGTAATTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGTAAAACCTATAAAGTCACCACTGAAGAGGAATTACGCGCCGCCCTTGCCGATGCGAAAAAACAAACCGTCTCTACGTTGATTGACATAAAAGTCTTGCCAAAAACCATGATTCACGGCTACGGCAGCTGGTGGCACGTTGGCGTAGCAGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCATAAAAGCAGCGTGAAACACATTAACGAAGCAAGACGCTATTAATCCG‑‑ATTCAATTTATTATCTCCAATTTAACCACTCTGTTGAGAAGGAAAACACTATGAAAGCTGAAAACGTGAAATTAGGTATCGCGCCAAT  >  1/1972691‑1973177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑