Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,972,881	T→C	T581T (ACT→ACC)	unknown →	3D‑(3,5/4)‑trihydroxycyclohexane‑1,2‑dione hydrolase (EC 3.7.1.22)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,972,881	0	T	C	100.0%	204.5 / NA	69	T581T (ACT→ACC)	unknown	3D‑(3,5/4)‑trihydroxycyclohexane‑1,2‑dione hydrolase (EC 3.7.1.22)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/27);  total (42/27)

CTTACATGATGCTCCATTCCGAACTGGTGACCTCCGTTCAGGAACATAAAAAAATCAACGTAATTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGTAAAACCTATAAAGTCACCACTGAAGAGGAATTACGCGCCGCCCTTGCCGATGCGAAAAAACAAACCGTCTCTACGTTGATTGACATAAAAGTCTTGCCAAAAACCATGATTCACGGCTACGGCAGCTGGTGGCACGTTGGCGTAGCAGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCATAAAAGCAGCGTGAAACACATTAACGAAGCAAGACGCTATTAATCCG‑‑ATTCAATTTATTATCTCCAATTTAACCACT  >  1/1972634‑1973119                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 cTTACATGATGCTCCATTCCGAACTGGTGACCTCCGTTCAGGAACATAAAAAAATCAACGTAATTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCGATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGaa                                                                                                                                                                                                                                               >  1:75943/1‑251 (MQ=255)  ttACATGATGCTCCATTCCGAACTGGTGACCTCCGTTCAGGAACATAAAAAAATCAACGTAATTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAag                                                                                                                                                                                                                                              >  1:215848/1‑251 (MQ=255)                aTTCCGAACTGGTGACCTCCGTTCAGGAACATAAAAAAATCAACGTAATTTTATTCGACAACATTACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACgcgc                                                                                                                                                                                                                                 >  1:226296/1‑250 (MQ=255)                aTTCCGAACTGGTGACCTCCGTTCAGGAACATAAAAAAATCAACGTAATTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTAc                                                                                                                                                                                                                                     >  1:280817/1‑246 (MQ=255)                aTTCCGAACTGGTGACCTCCGTTCAGGAACATAAAAAAATCAACGTAATTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACgcgc                                                                                                                                                                                                                                 >  1:182126/1‑250 (MQ=255)                                  ccGTTCAGGAACATAAAAAAATCAACGTAATTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTAcg                                                                                                                                                                                                                                    <  1:506500/229‑1 (MQ=255)                                                          aCGTAATTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACgc                                                                                                                                                                                           >  1:664245/1‑245 (MQ=255)                                                          aCGTAATTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGctgatt                                                                                                                                                                                      >  1:53009/1‑251 (MQ=255)                                                           cGTAATTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGctgattg                                                                                                                                                                                     <  1:576182/251‑1 (MQ=255)                                                               aTTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACAt                                                                                                                                                                                 >  1:617945/1‑251 (MQ=255)                                                               aTTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACAt                                                                                                                                                                                 >  1:855528/1‑251 (MQ=255)                                                               aTTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACAt                                                                                                                                                                                 <  1:854787/251‑1 (MQ=255)                                                                    aTTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAg                                                                                                                                                                            <  1:68296/251‑1 (MQ=255)                                                                     ttCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGt                                                                                                                                                                           >  1:489500/1‑251 (MQ=255)                                                                                 aCAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAAc                                                                                                                                                               <  1:118791/251‑1 (MQ=255)                                                                                        gCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGcc                                                                                                                                                                                                                       >  1:286092/1‑188 (MQ=255)                                                                                           gTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGAtcca                                                                                                                                                      >  1:793109/1‑247 (MQ=255)                                                                                             aTTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACgcgc                                                                                                                                                                                                                                 >  1:73720/1‑173 (MQ=255)                                                                                              ttAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGc                                                                                                                                                  <  1:468516/251‑1 (MQ=255)                                                                                               tAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCt                                                                                                                                                 <  1:689276/251‑1 (MQ=255)                                                                                                   aaTCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACgg                                                                                                                                             <  1:403900/251‑1 (MQ=255)                                                                                                                 cAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCAc                                                                                                                                <  1:570390/250‑1 (MQ=255)                                                                                                                    tGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGtcg                                                                                                                            >  1:36858/1‑249 (MQ=255)                                                                                                                     ggTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGGATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGc                                                                                                                                                  <  1:100384/228‑1 (MQ=255)                                                                                                                     ggTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACgc                                                                                                                                                                                           <  1:396553/187‑2 (MQ=255)                                                                                                                     ggTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAAc                                                                                                                                                               >  1:33176/1‑215 (MQ=255)                                                                                                                     ggTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGtcgg                                                                                                                           >  1:818479/1‑248 (MQ=255)                                                                                                                     ggTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGtcgg                                                                                                                           >  1:538095/1‑248 (MQ=255)                                                                                                                     ggTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGtcgg                                                                                                                           >  1:378984/1‑248 (MQ=255)                                                                                                                     ggTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGCGGCACGtcgg                                                                                                                           >  1:155157/1‑248 (MQ=255)                                                                                                                     ggTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATCGACATAAAAGTCTTGCCGAAAACCATGATCCACGGc                                                                                                                                                  >  1:873282/1‑228 (MQ=255)                                                                                                                                cTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGtgccggaag                                                                                                                <  1:206278/251‑9 (MQ=255)                                                                                                                                                  cTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGaaaaaa                                                                                              <  1:805843/251‑1 (MQ=255)                                                                                                                                                    tCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAAcc                                                                                                                                                              <  1:585812/185‑1 (MQ=255)                                                                                                                                                    tCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAAt                                                                                             >  1:29550/1‑250 (MQ=255)                                                                                                                                                    tCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAAt                                                                                             >  1:131724/1‑250 (MQ=255)                                                                                                                                                                   ccAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACaaa                                                                             <  1:806101/251‑1 (MQ=255)                                                                                                                                                                   ccAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACaaa                                                                             >  1:502984/1‑251 (MQ=255)                                                                                                                                                                     aaTAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACaaaaa                                                                           >  1:404485/1‑250 (MQ=255)                                                                                                                                                                      aTAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCATCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACaaaaac                                                                          <  1:456292/251‑3 (MQ=255)                                                                                                                                                                        aaaTTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAaacag                                                                        <  1:761364/251‑1 (MQ=255)                                                                                                                                                                        aaaTTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAaacag                                                                        >  1:510202/1‑251 (MQ=255)                                                                                                                                                                          aTTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCg                                                                      <  1:517368/251‑1 (MQ=255)                                                                                                                                                                          aTTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCg                                                                      >  1:26199/1‑251 (MQ=255)                                                                                                                                                                                  gcggATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAaca                                                               >  1:433129/1‑250 (MQ=255)                                                                                                                                                                                      aTTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACAtt                                                           <  1:742173/250‑1 (MQ=255)                                                                                                                                                                                       ttCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGAtc                                                                                                                                                        >  1:32937/1‑155 (MQ=255)                                                                                                                                                                                        tCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAAc                                                        <  1:497589/251‑1 (MQ=255)                                                                                                                                                                                        tCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAAc                                                        <  1:522534/251‑1 (MQ=255)                                                                                                                                                                                           tGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAacac                                                              <  1:145380/242‑1 (MQ=255)                                                                                                                                                                                           tGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGa                                                      <  1:707976/250‑1 (MQ=255)                                                                                                                                                                                           tGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGa                                                      <  1:208526/250‑1 (MQ=255)                                                                                                                                                                                            gCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAGAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAg                                                    >  1:841229/1‑251 (MQ=255)                                                                                                                                                                                               cGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAGCACATTAACGAAGCaa                                                 >  1:857660/1‑251 (MQ=255)                                                                                                                                                                                                         gCCATGAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGttatta                                       >  1:293476/1‑251 (MQ=255)                                                                                                                                                                                                              gAATGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCg                                  >  1:496014/1‑251 (MQ=255)                                                                                                                                                                                                                aTGCGGCATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAacac                                                              >  1:134178/1‑221 (MQ=255)                                                                                                                                                                                                                      cATCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCgtcatttaa                          <  1:562133/251‑9 (MQ=255)                                                                                                                                                                                                                       aTCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGGCATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAAt                                     >  1:165400/1‑239 (MQ=255)                                                                                                                                                                                                                       aTCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATc                                    <  1:241425/240‑1 (MQ=255)                                                                                                                                                                                                                        tCTTACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCgtcatttaatt                        >  1:722259/1‑241 (MQ=255)                                                                                                                                                                                                                           tACGGTTGCAAAACCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCgtcatttaatttat                     <  1:93101/251‑14 (MQ=255)                                                                                                                                                                                                                                ttgcaaaaCCTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATttatttct                 >  1:750369/5‑247 (MQ=255)                                                                                                                                                                                                                                        ccTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACaaa                                                                             >  1:163169/1‑182 (MQ=255)                                                                                                                                                                                                                                        ccTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTaa                                      >  1:458110/1‑221 (MQ=255)                                                                                                                                                                                                                                        ccTATAAAGTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTaa       >  1:27392/1‑251 (MQ=255)                                                                                                                                                                                                                                                gTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAAcc     >  1:261664/1‑245 (MQ=255)                                                                                                                                                                                                                                                gTCACCACCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAACCAct  >  1:807602/1‑248 (MQ=255)                                                                                                                                                                                                                                                    ccacCGAAGAGGAATTACGCGCCGCCCTCGCCGATGCGCAAAAACAAACCGTCTCTACGCTGATTGACATAAAAGTCTTGCCGAAAACCATGATCCACGGCTACGGCAGCTGGTGGCACGTCGGCGTGCCGGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCACAAAAACAGCGTGAAACACATTAACGAAGCAAGACGTTATTAATCCGTCATTTAATTTATT‑TCTCCAATTTAAcc     >  1:76704/1‑241 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 CTTACATGATGCTCCATTCCGAACTGGTGACCTCCGTTCAGGAACATAAAAAAATCAACGTAATTTTATTCGACAACATGACAAACGGCTGTATTAACAATCTGCAAATCGGCAATGGTATGGACAGCTTCGCCACCGAATTCCGCTTCAGAAATCCGCAAACCAATAAATTAGACGGCGGATTCGTGCCCGTTGACTTTGCCATGAATGCGGCATCTTACGGTTGTAAAACCTATAAAGTCACCACTGAAGAGGAATTACGCGCCGCCCTTGCCGATGCGAAAAAACAAACCGTCTCTACGTTGATTGACATAAAAGTCTTGCCAAAAACCATGATTCACGGCTACGGCAGCTGGTGGCACGTTGGCGTAGCAGAAGTGTCGGAAAAAGAAAAAATCCGCAAAGCGCATAAAAGCAGCGTGAAACACATTAACGAAGCAAGACGCTATTAATCCG‑‑ATTCAATTTATTATCTCCAATTTAACCACT  >  1/1972634‑1973119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑