Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,965,158	T→C	A280A (GCA→GCG)	unknown ←	Uncharacterized MFS‑type transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,965,158	0	T	C	100.0%	211.7 / NA	75	A280A (GCA→GCG)	unknown	Uncharacterized MFS‑type transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/43);  total (32/43)

CCAATAAACCGCCTAAGAAACATAATTGAATACCGAGCGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCTGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATACTTCTTTATTATCGAAAGTTTCTTCTTTTGATTCGGTTTTAATAATCGGTTTAAATCCGCAGGATTCCGGCGAATCTTTCATGAACATGATGG  >  1/1964914‑1965399                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  ccAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCa                                                                                                                                                                                                                                             >  1:799766/1‑251 (MQ=255)     tAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCAATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAAtgg                                                                                                                                                                                                                                         <  1:687026/251‑1 (MQ=255)                    aCATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGc                                                                                                                                                                                                                          <  1:360635/251‑1 (MQ=255)                               tACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAgg                                                                                                                                                                                                               >  1:312493/1‑251 (MQ=255)                                   gagtgTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGc                                                                                                                                                                                                                          <  1:778141/232‑1 (MQ=255)                                     gtgTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAAt                                                                                                                                                                                                         >  1:678674/3‑251 (MQ=255)                                        tAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATAc                                                                                                                                                                                                       <  1:761287/250‑1 (MQ=255)                                           tACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGccc                                                                                                                                                                                                   <  1:753541/251‑1 (MQ=255)                                                  gACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGcc                                                                                                                                                                                                                               >  1:808275/1‑216 (MQ=255)                                                    cGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTc                                                                                                                                                                                          <  1:476302/251‑1 (MQ=255)                                                           tGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGcc                                                                                                                                                                                     <  1:474994/249‑1 (MQ=255)                                                               ataatGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGGAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACg                                                                                                                                                                               >  1:504119/1‑251 (MQ=255)                                                                aaatGGAGAACCAGGTATTGCCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACGGACCCCGATGGTCATGATATTCCGTCTCCCCTTTAAGAAACGATCGGAAAACCAGCCTAAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAAAAAAAAAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACgg                                                                                                                                                                              <  1:266694/250‑1 (MQ=255)                                                                  atGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGc                                                                                                                                                                            <  1:142268/251‑1 (MQ=255)                                                                       gaACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAatat                                                                                                                                                                       >  1:582858/1‑251 (MQ=255)                                                                        aaCCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAatat                                                                                                                                                                       <  1:604373/250‑1 (MQ=255)                                                                        aaCCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAAtata                                                                                                                                                                      <  1:816935/251‑1 (MQ=255)                                                                         aCCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAAtgg                                                                                                                                                                                                                                         >  1:183530/1‑183 (MQ=255)                                                                            aGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATAc                                                                                                                                                                                                       <  1:854575/214‑1 (MQ=255)                                                                             ggTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTc                                                                                                                                                                                                                   >  1:324311/1‑201 (MQ=255)                                                                             ggTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCaa                                                                                                                                                                          <  1:197273/242‑1 (MQ=255)                                                                             ggTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATatacata                                                                                                                                                                  <  1:860362/250‑1 (MQ=255)                                                                              gTATTCCCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGCCCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATatacatac                                                                                                                                                                 <  1:248370/250‑1 (MQ=255)                                                                              gTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAAtgg                                                                                                                                                                                                                                         <  1:344437/178‑1 (MQ=255)                                                                              gTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCt                                                                                                                                                                                                                              >  1:773478/1‑189 (MQ=255)                                                                              gTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATatacat                                                                                                                                                                   <  1:560743/248‑1 (MQ=255)                                                                              gTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATAtacataca                                                                                                                                                                >  1:193068/1‑251 (MQ=255)                                                                              gTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATAtacataca                                                                                                                                                                >  1:161270/1‑251 (MQ=255)                                                                                gttACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATatacatac                                                                                                                                                                 <  1:839651/247‑1 (MQ=255)                                                                                      gTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCaa                                                                                                                                                                                                                                            <  1:128385/167‑1 (MQ=255)                                                                                               gAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGcc                                                                                                                                                                                     >  1:519712/1‑213 (MQ=255)                                                                                                 aaaagaagGCGGCAAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGTTCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCACCTGTTCACGCCATAACGGGCAATatac                                                                                                                                                                     <  1:581671/225‑1 (MQ=255)                                                                                                  tAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAAtggtgg                                                                                                                                                                                                                                      <  1:249572/161‑1 (MQ=255)                                                                                                  tAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGc                                                                                                                                                                            >  1:125950/1‑219 (MQ=255)                                                                                                                  gAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGAtt                                                                                                                            >  1:651588/1‑251 (MQ=255)                                                                                                                              aGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAAtgg                                                                                                                                                                                                                                         <  1:791469/130‑1 (MQ=255)                                                                                                                                   aCTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATatacatac                                                                                                                                                                 <  1:882388/197‑1 (MQ=255)                                                                                                                                      gACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAatat                                                                                                                                                                       >  1:658574/1‑188 (MQ=255)                                                                                                                                        ccccGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCAc                                                                                                                   <  1:893665/238‑1 (MQ=255)                                                                                                                                               ggTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTg                                                                                                                                                                                             >  1:577627/1‑157 (MQ=255)                                                                                                                                                 tCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCt                                                                                                                                                                                                                              <  1:234321/122‑1 (MQ=255)                                                                                                                                                  cATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAAt                                                                                                                                     <  1:201524/210‑1 (MQ=255)                                                                                                                                                   aTGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATActtc                                                                                           <  1:526676/251‑1 (MQ=255)                                                                                                                                                   aTGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATActt                                                                                            >  1:852075/1‑250 (MQ=255)                                                                                                                                                           ccGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATatacatac                                                                                                                                                                 <  1:651185/173‑1 (MQ=255)                                                                                                                                                           ccGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATc                                                                                                                                    <  1:889883/202‑1 (MQ=255)                                                                                                                                                                 aCCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATAc                                                                                              <  1:556154/234‑1 (MQ=255)                                                                                                                                                                  cccTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATACTTCTttat                                                                                      <  1:648615/241‑1 (MQ=255)                                                                                                                                                                      gTAAGAAACGATCGGATAACCAGCCTGAAGCCCCCGTTCCCAGAATACCGACAATACTGTTTCCGCTAATAATAGAAGCCGCTCCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATatacatac                                                                                                                                                                 <  1:899302/162‑1 (MQ=255)                                                                                                                                                                      gTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATatacata                                                                                                                                                                  <  1:284180/161‑1 (MQ=255)                                                                                                                                                                      gTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATatacata                                                                                                                                                                  <  1:495077/161‑1 (MQ=255)                                                                                                                                                                                aTCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATACTTCTTTATTATCGAAAGTTTCTTCTTTTGAtt                                                              >  1:513947/1‑251 (MQ=255)                                                                                                                                                                                aTCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATACTTCTTTATTATCGAAAGTTTCTTCTTTTGAtt                                                              <  1:76085/251‑1 (MQ=255)                                                                                                                                                                                  cGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGcc                                                                                                                                                                                                                               >  1:896625/1‑88 (MQ=255)                                                                                                                                                                                  cGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACaagaag                                                                                                                                                        <  1:347636/159‑1 (MQ=255)                                                                                                                                                                                    gATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATCCGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGgtgt                                                                                                      <  1:28015/207‑1 (MQ=255)                                                                                                                                                                                           aGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATACTTCTTTATTATCGAAAGTTTCTTCTTTTGATTCGGTTTtaata                                                   >  1:832888/1‑251 (MQ=255)                                                                                                                                                                                           aGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATACTTCTTTATTATCGAAAGTTTCTTCTTTTGATTCGGTTTtaata                                                   >  1:664536/1‑251 (MQ=255)                                                                                                                                                                                            gCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATACTTCTTTATTATCGAAAGTTTCTTCTTTTGATTCGGTTTtaataa                                                  >  1:696311/1‑251 (MQ=255)                                                                                                                                                                                                gAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAAc                                                                                                                                                                                <  1:55801/121‑1 (MQ=255)                                                                                                                                                                                                gAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATatacatac                                                                                                                                                                 <  1:830841/136‑1 (MQ=255)                                                                                                                                                                                                 aaGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATatacatac                                                                                                                                                                 <  1:324081/135‑1 (MQ=255)                                                                                                                                                                                                                     ccGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAAt                                                                                                                                     >  1:511870/1‑143 (MQ=255)                                                                                                                                                                                                                     ccGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCAtt                                                                                                           >  1:449985/1‑169 (MQ=255)                                                                                                                                                                                                                        aCAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATACTTCTTTATTATCGAAAGTTTCTTCTTTTGATTCGGTTTTAATAATCGGTTTAAATCCGCAGGATTCCGGCGa                      >  1:261511/1‑251 (MQ=255)                                                                                                                                                                                                                              cTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATAc                                                                                              <  1:243491/173‑1 (MQ=255)                                                                                                                                                                                                                                gTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATACTTCTTTATTATCGAAAGTTTCTTCTTTTGATTCGGTTTtaata                                                   >  1:218066/1‑214 (MQ=255)                                                                                                                                                                                                                                gTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATACTTCTTTATTATCGAAAGTTTCTTCTTTTGATTCGGTTTTAATAATCGGTTTAAATCCGCAGGATTCCGGCGAATCTTTCa              >  1:755227/1‑251 (MQ=255)                                                                                                                                                                                                                                        taataaTAGAAGCCGCTTCAATGGTGGTGTAGCCt                                                                                                                                                                                                                              <  1:586549/35‑1 (MQ=255)                                                                                                                                                                                                                                         aataatAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATACTTCTTTATTATCGAAAGTTTCTTCTTTTGATTCGGTTTTAATAATCGGTTTAAATCCGCAGGATTCCGGCGAATCTTTCATGAACatga     >  1:181855/1‑251 (MQ=255)                                                                                                                                                                                                                                         aataatAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATACTTCTTTATTATCGAAAGTTTCTTCTTTTGATTCGGTTTTAATAATCGGTTTAAATCCGCAGGATTCCGGCGAATCTTTCATGAACatga     >  1:571367/1‑251 (MQ=255)                                                                                                                                                                                                                                          ataataGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATACTTCTTTATTATCGAAAGTTTCTTCTTTTGATTCGGTTTTAATAATCGGTTTAAATCCGCAGGATTCCGGCGAATCTTTCATGAACatgat    >  1:329950/1‑251 (MQ=255)                                                                                                                                                                                                                                           taataGAAGCCGCTTCAATGGTGGTGTAGCCTTTTCCGTTTTCCAGAAAAAATACGCCCCACTTGTTCACGCCATAACGGGCAATATACATTCAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATACTTCTTTATTATCGaa                                                                               <  1:83459/175‑1 (MQ=255)                                                                                                                                                                                                                                            aataGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATACTTCTTTATTATCGAAAGTTTCTTCTTTTGATTCGGTTTTAATAATCGGTTTAAATCCGCAGGATTCCGGCGAATCTTTCATGAACATGATgg  >  1:754314/1‑251 (MQ=255)                                                                                                                                                                                                                                             ataGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAAc                                                                                                                                                                                >  1:397636/1‑76 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  CCAATAAACCGCCTAAGAAACATAATTGAATACCGAGCGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCTGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGGAAAAATACGCCCCAACTGTTCACGCCATAACGGGCAATATACATACAACAAGAAGCGACTGCCAGGATCCAAATCGCCGGATTTTTTATCACTTCCCATTGGTGTTTTAATACTTCTTTATTATCGAAAGTTTCTTCTTTTGATTCGGTTTTAATAATCGGTTTAAATCCGCAGGATTCCGGCGAATCTTTCATGAACATGATGG  >  1/1964914‑1965399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑