Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,964,951	C→T	T349T (ACG→ACA)	unknown ←	Uncharacterized MFS‑type transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,964,951	0	C	T	100.0%	220.9 / NA	77	T349T (ACG→ACA)	unknown	Uncharacterized MFS‑type transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/36);  total (41/36)

TGCCAATACGGAGCACACATCGGCGCCGACCCAGAAATAGGACAAGCTGTCGAAATCATAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGCGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCTGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGG  >  1/1964708‑1965194                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    tGCCAATACGGAGCACACATCGGCGCCGACCCAGAAATAGGACAAGCTGTCGAAATCATAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATa                                                                                                                                                                                                                                               >  1:259243/1‑250 (MQ=255)   ccAATACGGAGCACACATCGGCGCCGACCCAGAAATAGGACAAGCTGTCGAAATCATAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCa                                                                                                                                                                                                                                            <  1:33006/251‑1 (MQ=255)           gAGCACACATCGGCGCCGACCCAGAAATAGGACAAGCTGTCGAAATCATAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGa                                                                                                                                                                                                                                    >  1:239241/1‑251 (MQ=255)             gCACACATCGGCGCCGACCCAGAAATAGGACAAGCTGTCGAAATCATAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGaaa                                                                                                                                                                                                                                  >  1:163800/1‑251 (MQ=255)                  caTCGGCGCCGACCCAGAAATAGGACAAGCTGTCGAAATCATAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCa                                                                                                                                                                                                                             >  1:217686/1‑251 (MQ=255)                       gcgcCGACCCAGAAATAGGACAAGCTGTCGAAATCATAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCaataa                                                                                                                                                                                                                         <  1:209762/250‑1 (MQ=255)                              cccAGAAATAGGACAAGCTGTCGAAATCATAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGaa                                                                                                                                                                                                                  >  1:729869/1‑250 (MQ=255)                                cAGAAATAGGACAAGCTGTCGAAATCATAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCa                                                                                                                                                                                                               >  1:229253/1‑251 (MQ=255)                                   aaaTAGGACAAGCTGTCGAAATCATAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGt                                                                                                                                                                                                            <  1:632288/251‑1 (MQ=255)                                        ggACAAGCTGTCGAAATCATAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTa                                                                                                                                                                                                        >  1:563372/1‑250 (MQ=255)                                          aCAAGCTGTCGAAATCATAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCg                                                                                                                                                                                                     <  1:874900/251‑1 (MQ=255)                                          aCAAGCTGTCGAAATCATAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCg                                                                                                                                                                                                     <  1:221486/251‑1 (MQ=255)                                              gCTGTCGAAATCATAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAAc                                                                                                                                                                                                                 >  1:332293/1‑235 (MQ=255)                                                 gTCGAAATCATAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCTCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGgag                                                                                                                                                                                                                    >  1:517303/1‑229 (MQ=255)                                                           tAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATAc                                                                                                                                                                                                                                              <  1:565208/193‑1 (MQ=255)                                                           tAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATaagaa                                                                                                                                                                                    <  1:270696/251‑1 (MQ=255)                                                           tAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATaagaa                                                                                                                                                                                    <  1:584914/251‑1 (MQ=255)                                                                             tCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCaataa                                                                                                                                                                                                                         <  1:604771/196‑1 (MQ=255)                                                                             tCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATgg                                                                                                                                                                                                                      >  1:217312/1‑199 (MQ=255)                                                                             tCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGgag                                                                                                                                                                                                                    <  1:300208/201‑1 (MQ=255)                                                                             tCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATa                                                                                                                                                                                        >  1:108506/1‑229 (MQ=255)                                                                             tCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGa                                                                                                                                                                   >  1:747838/1‑250 (MQ=255)                                                                             tCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGa                                                                                                                                                                   >  1:600636/1‑250 (MQ=255)                                                                             tCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGCGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGa                                                                                                                                                                   >  1:460313/1‑250 (MQ=255)                                                                               aTTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGt                                                                                                                                                                >  1:535120/1‑251 (MQ=255)                                                                                       ccGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCaaa                                                                                                                                                        >  1:200516/1‑251 (MQ=255)                                                                                            gTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGAc                                                                                                                                                                                                                                       <  1:474754/167‑1 (MQ=255)                                                                                            gTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGa                                                                                                                                                    >  1:63140/1‑250 (MQ=255)                                                                                                 aTCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGa                                                                                                                                              >  1:280447/1‑251 (MQ=255)                                                                                                    tAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATgg                                                                                                                                           <  1:682377/251‑1 (MQ=255)                                                                                                     aaCATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATAcc                                                                                                                                                                                                                                             <  1:295630/152‑1 (MQ=255)                                                                                                           aaGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGat                                                                                                                                    <  1:864967/251‑1 (MQ=255)                                                                                                             gCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATgg                                                                                                                                           <  1:138684/242‑1 (MQ=255)                                                                                                               cGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTc                                                                                                                                <  1:673848/251‑1 (MQ=255)                                                                                                                     gaAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGt                                                                                                                                                                                                            <  1:534463/169‑1 (MQ=255)                                                                                                                      aaaCTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCgggg                                                                                                                                                                                              <  1:243881/182‑1 (MQ=255)                                                                                                                         cTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTaa                                                                                                                                                                                                                                                 <  1:237181/128‑1 (MQ=255)                                                                                                                              cTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAg                                                                                                                 <  1:302610/251‑1 (MQ=255)                                                                                                                                      gcACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGgag                                                                                                                                                                                                                    >  1:665261/1‑144 (MQ=255)                                                                                                                                      gcACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGAAATAATgg                                                                                                                                                                                                                      >  1:186631/1‑142 (MQ=255)                                                                                                                                         ccGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCaataa                                                                                                                                                                                                                         <  1:347975/136‑1 (MQ=255)                                                                                                                                            gCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAg                                                                                                                 >  1:670257/1‑237 (MQ=255)                                                                                                                                               tAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCa                                                                                                >  1:417547/1‑251 (MQ=255)                                                                                                                                                 aCCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAg                                                                                               >  1:26579/1‑250 (MQ=255)                                                                                                                                                    gAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATaagaag                                                                                                                                                                                   <  1:147355/163‑1 (MQ=255)                                                                                                                                                    gAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGc                                                                                              >  1:534062/1‑248 (MQ=255)                                                                                                                                                       tACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAg                                                                                        <  1:805390/251‑1 (MQ=255)                                                                                                                                                        aCGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAg                                                                                        >  1:359891/1‑250 (MQ=255)                                                                                                                                                            ccATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAAGACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCAcc                                                                                   <  1:425303/251‑1 (MQ=255)                                                                                                                                                             catcatGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCg                                                                                  >  1:192880/1‑251 (MQ=255)                                                                                                                                                             catcatGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCg                                                                                  <  1:763805/251‑1 (MQ=255)                                                                                                                                                                  tGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTcc                                                                              >  1:286208/1‑250 (MQ=255)                                                                                                                                                                  tGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTcc                                                                              >  1:368736/1‑250 (MQ=255)                                                                                                                                                                   gCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATAc                                                                                                                                                                                                                                              >  1:406772/1‑89 (MQ=255)                                                                                                                                                                   gCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCa                                                                            >  1:843815/1‑251 (MQ=255)                                                                                                                                                                         tGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGat                                                                                                                                    <  1:493887/189‑1 (MQ=255)                                                                                                                                                                          gCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATAc                                                                      >  1:364733/1‑250 (MQ=255)                                                                                                                                                                             aTACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGAc                                                                  <  1:758299/251‑1 (MQ=255)                                                                                                                                                                              tACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACa                                                                 <  1:813300/251‑1 (MQ=255)                                                                                                                                                                                       cGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGttt                                                        <  1:818851/251‑1 (MQ=255)                                                                                                                                                                                         gATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTa                                                       >  1:361224/1‑250 (MQ=255)                                                                                                                                                                                           tttATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGCTATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACg                                                     >  1:439621/1‑250 (MQ=255)                                                                                                                                                                                           tttATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGc                                                    <  1:355663/251‑1 (MQ=255)                                                                                                                                                                                                     aTGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGa                                          <  1:3416/251‑1 (MQ=255)                                                                                                                                                                                                               ccAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATaagaa                                                                                                                                                                                    >  1:346852/1‑103 (MQ=255)                                                                                                                                                                                                               ccAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTAccc                                                                                                                       >  1:879238/1‑164 (MQ=255)                                                                                                                                                                                                               ccAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGa                                                                   <  1:632996/216‑1 (MQ=255)                                                                                                                                                                                                               ccAATAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCa                                >  1:799766/1‑251 (MQ=255)                                                                                                                                                                                                                   tAAACCGCCTAAGAAACATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCAATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAAtgg                            <  1:687026/251‑1 (MQ=255)                                                                                                                                                                                                                                  aCATAATTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGc             <  1:360635/251‑1 (MQ=255)                                                                                                                                                                                                                                       aTTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCa                                                                                                                                                                                                               >  1:85791/1‑52 (MQ=255)                                                                                                                                                                                                                                       aTTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTaa                                                                                                                  >  1:633024/1‑145 (MQ=255)                                                                                                                                                                                                                                       aTTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCg                                                                                  >  1:370865/1‑177 (MQ=255)                                                                                                                                                                                                                                       aTTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCt                                                   <  1:614354/208‑1 (MQ=255)                                                                                                                                                                                                                                       aTTGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGa                                          >  1:5567/1‑217 (MQ=255)                                                                                                                                                                                                                                        ttGAATACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAgc                                       <  1:318838/219‑1 (MQ=255)                                                                                                                                                                                                                                             tACCGAGTGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCCGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAgg  >  1:312493/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    TGCCAATACGGAGCACACATCGGCGCCGACCCAGAAATAGGACAAGCTGTCGAAATCATAAATTTTCTTACCGGCTTCATTAATCACCGTGGTTTTATCTAACATAAAGCCGGTGAGAAACTCCCCTGCCGCCGCACCGGCATAACCGAATACGCCCATCATGCCTAATGCGATACCAGATGCGGATTTATGGGAAATGTCCGTCGCCAATAAACCGCCTAAGAAACATAATTGAATACCGAGCGTAATACCAAAGACGGAAAGTGCAATAATGGAGAACCAGGTATTACCGTTCGGGGCGAATAAGAAGGCTGCCAAAGAAATGACGTTAAGCAAACTGACCCCGATGGTCATGATATTCCGTCTACCCTGTAAGAAACGATCGGATAACCAGCCTGAAGCCACCGTTCCCAGAATACCGACAATACTGTTTACGCTAATAATAGAAGCTGCTTCAATGGTGGTGTAGCCTTTGCCGTTTTCCAGG  >  1/1964708‑1965194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑