Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,958,927	G→A	T143T (ACC→ACT)	unknown ←	Inositol transport system ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,958,927	0	G	A	100.0%	231.5 / NA	81	T143T (ACC→ACT)	unknown	Inositol transport system ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/36);  total (45/36)

AATGACGGTAATCTCATCAGATACACGGAAAACTTCATCTAATTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCGGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATAAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCAT  >  1/1958693‑1959165                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               aaTGACGGTAATCTCATCAGATACACGGAAAACTTCATCTAATTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCa                                                                                                                                                                                                                                >  1:437025/1‑251 (MQ=255)   tGACGGTAATCTCATCAGATACACGGAAAACTTCATCTAATTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACg                                                                                                                                                                                                                              >  1:398654/1‑251 (MQ=255)          aaTCTCATCAGATACACGGAAAACTTCATCTAATTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAg                                                                                                                                                                                                                         >  1:568762/1‑249 (MQ=255)           acttcatcaatttcaaggAAAACTTATTCTAATTAATGGTAAAAAAAAAAAATCCGGCGGCCTCTTTCTTTTGCCGTTCCAAAAAGGCGGTAAAGCTGCCCGCCCCCATTTTCCGTGAATGCCGTGTTCGTGTCGTCCATATAGATTACTTTGGCTTCATAAGAATCCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGctc                                                                                                                                                                                                                      <  1:280030/235‑1 (MQ=255)             ctcATCAGATACACGGAAAACTTCATCTAATTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCtt                                                                                                                                                                                                                    >  1:105652/1‑251 (MQ=255)               catcaGATCCACGGAAAACTTCATCTAATTTATGGAAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTACAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTAAATGCCGTGGTGGGCTCGTCCATAATGATGTCTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCAt                                                                                                                                                                                                                                     <  1:458926/232‑1 (MQ=255)               catcaGATACACGGAAAACTTCATCTAATTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCtttt                                                                                                                                                                                                                  >  1:129566/1‑251 (MQ=255)               catcaGATACACGGAAAACTTCATCTAATTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCtttt                                                                                                                                                                                                                  >  1:338802/1‑251 (MQ=255)                atcaGATACACGGAAAACTTCATCTAATTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTACGTCAATGTCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGTAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGCCCATCGGCACGCCAAGCTCTTTTa                                                                                                                                                                                                                 >  1:86547/1‑251 (MQ=255)                atcaGATACACGGAAAACTTCATCTAATTTATGGAAAATAAAAATAATCGCGATGCCTCTTTTTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCACCCTCATTTTCCGACAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTa                                                                                                                                                                                                                 <  1:92900/251‑1 (MQ=255)                    gATACACGGAAAACTTCATCTAATTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAatat                                                                                                                                                                                                             >  1:586068/1‑251 (MQ=255)                     aTACACGGAAAACTTCATCTAATTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTCCCGTTTCAATAATGCGGTAAAGCTGCTCGCCCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAat                                                                                                                                                                                                               <  1:481611/248‑1 (MQ=255)                     aTACACGGAAAACTTCATCTAATTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAatat                                                                                                                                                                                                             <  1:635715/250‑1 (MQ=255)                                 cTTCATCTAATTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTg                                                                                                                                                                                                 >  1:589756/1‑250 (MQ=255)                                   tCATCTAATTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCt                                                                                                                                                                                               >  1:488681/1‑250 (MQ=255)                                   tCATCTAATTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTc                                                                                                                                                                                              <  1:138914/251‑1 (MQ=255)                                         aaTTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGc                                                                                                                                                                                                                                 <  1:469251/210‑1 (MQ=255)                                         aaTTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCAt                                                                                                                                                                                        >  1:610566/1‑251 (MQ=255)                                            ttATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGc                                                                                                                                                                                     <  1:757907/251‑1 (MQ=255)                                                    aataaaaataaTCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGAt                                                                                                                                                                             <  1:332380/251‑1 (MQ=255)                                                                        ctctTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAatat                                                                                                                                                                                                             <  1:201691/199‑1 (MQ=255)                                                                            ttctttTCCCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGa                                                                                                                                                                              <  1:867590/226‑1 (MQ=255)                                                                            ttctttTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGcc                                                                                                                                                                                                   >  1:128294/1‑205 (MQ=255)                                                                            ttctttTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGa                                                                                                                                                                              >  1:479436/1‑226 (MQ=255)                                                                            ttctttTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGt                                                                                                                                                       >  1:824473/1‑249 (MQ=255)                                                                            ttctttTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCCCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTaa                                                                                                                                                     >  1:299864/1‑251 (MQ=255)                                                                                    ccGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTc                                                                                                                                                                                 <  1:360890/215‑1 (MQ=255)                                                                                     cGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGcc                                                                                                                                            <  1:468542/251‑1 (MQ=255)                                                                                     cGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGc                                                                                                                                             >  1:7561/1‑250 (MQ=255)                                                                                          cAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTa                                                                                                                                                                                                                 >  1:174283/1‑177 (MQ=255)                                                                                          cAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAg                                                                                                                                       >  1:712455/1‑251 (MQ=255)                                                                                            ataatGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAAc                                                                                                                                                                  >  1:497581/1‑222 (MQ=255)                                                                                               atGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGTAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAActtggct                                                                                                                                                            >  1:288279/1‑225 (MQ=255)                                                                                                 gCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCAt                                                                                                                                                                                        >  1:588663/1‑195 (MQ=255)                                                                                                 gCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAAt                                                                                                                                >  1:259280/1‑251 (MQ=255)                                                                                                  cGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAAtt                                                                                                                               >  1:862473/1‑251 (MQ=255)                                                                                                   ggTAAAGCTGCTCGCCCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGct                                                                                                                                                                                                                       <  1:586787/162‑1 (MQ=255)                                                                                                      aaaGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCg                                                                                                                           >  1:537380/1‑251 (MQ=255)                                                                                                                 aCCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTaaaaa                                                                                                                                 >  1:792568/1‑234 (MQ=255)                                                                                                                 aCCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCaa                                                                                                                >  1:740177/1‑251 (MQ=255)                                                                                                                     cATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTg                                                                                                            >  1:345770/1‑251 (MQ=255)                                                                                                                             gTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTaaaaa                                                                                                                                 >  1:756324/1‑222 (MQ=255)                                                                                                                                 aTGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGaa                                                                                                >  1:680600/1‑251 (MQ=255)                                                                                                                                   gCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAAt                                                                                              >  1:233860/1‑251 (MQ=255)                                                                                                                                       tGGTCGGCTCGTCCATAAGGATGACTTTGGCGTCATTAAAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTc                                                                                           <  1:244074/250‑1 (MQ=255)                                                                                                                                          tCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGc                                                                                       <  1:713162/251‑1 (MQ=255)                                                                                                                                            ggCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAata                                                                                                                                                                                                              <  1:714021/130‑1 (MQ=255)                                                                                                                                            ggCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCgg                                                                                     >  1:461993/1‑251 (MQ=255)                                                                                                                                            ggCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCgg                                                                                     >  1:474086/1‑251 (MQ=255)                                                                                                                                             gCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATc                                                                                                                                                                                                                                    <  1:834820/107‑1 (MQ=255)                                                                                                                                               tCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCAc                                                                                  <  1:258748/251‑1 (MQ=255)                                                                                                                                                cGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACg                                                                                                                                              >  1:187054/1‑190 (MQ=255)                                                                                                                                                 gTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAAtt                                                                                                             >  1:258120/1‑222 (MQ=255)                                                                                                                                                 gTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGaa                                                                                                <  1:312207/235‑1 (MQ=255)                                                                                                                                                 gTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCa                                                                                >  1:663303/1‑251 (MQ=255)                                                                                                                                                   ccATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATg                                                                              >  1:100051/1‑251 (MQ=255)                                                                                                                                                     aTAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAAttt                                                                                            >  1:376232/1‑235 (MQ=255)                                                                                                                                                      tAATGAGGATTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTAGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGtt                                                                           <  1:461150/251‑1 (MQ=255)                                                                                                                                                       aaTGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCAc                                                                                                                                                                                                                               <  1:149758/102‑1 (MQ=255)                                                                                                                                                        atgatgACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTaa                                                                         >  1:326065/1‑251 (MQ=255)                                                                                                                                                           atgaCTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGcc                                                                      <  1:165458/251‑1 (MQ=255)                                                                                                                                                           atgaCTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGc                                                                       <  1:863384/250‑1 (MQ=255)                                                                                                                                                             gaCTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAat                                                                                                                                                                                                               <  1:409333/112‑1 (MQ=255)                                                                                                                                                                tttGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAAc                                                                 <  1:681963/251‑1 (MQ=255)                                                                                                                                                                         aTTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAAttc                                                                                                                                                  >  1:382157/1‑160 (MQ=255)                                                                                                                                                                         aTTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCAt                                                        >  1:584375/1‑251 (MQ=255)                                                                                                                                                                          ttAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTACGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCAt                                                        >  1:427828/1‑250 (MQ=255)                                                                                                                                                                               aaCCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGTAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAg                                                  <  1:492298/251‑1 (MQ=255)                                                                                                                                                                                            atagcaaCTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGttt                                     <  1:404180/251‑1 (MQ=255)                                                                                                                                                                                              agcaaCTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAAttt                                                                                                                                                  <  1:877677/140‑1 (MQ=255)                                                                                                                                                                                                         gcgcTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAat                                                                                                                                                                                                               <  1:137089/68‑1 (MQ=255)                                                                                                                                                                                                            cTTTTGCCACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTc                      >  1:641460/1‑250 (MQ=255)                                                                                                                                                                                                                  ccACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAaat                <  1:744952/250‑1 (MQ=255)                                                                                                                                                                                                                   cACCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATAGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTaaaaa                                                                                                                                 <  1:193533/136‑1 (MQ=255)                                                                                                                                                                                                                    aCCGATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGcc                                                                      <  1:308882/194‑1 (MQ=255)                                                                                                                                                                                                                     cagATACATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAg                                 >  1:34955/3‑230 (MQ=255)                                                                                                                                                                                                                           cATCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCg      >  1:877103/1‑251 (MQ=255)                                                                                                                                                                                                                            aTCGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGCCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGc     <  1:71263/251‑1 (MQ=255)                                                                                                                                                                                                                              cGGACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCca   <  1:132475/251‑1 (MQ=255)                                                                                                                                                                                                                               ggACATTTTGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATCAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCcat  >  1:168849/1‑251 (MQ=255)                                                                                                                                                                                                                                    ttttGGCAGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCAc                                                                                  <  1:356078/166‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                               AATGACGGTAATCTCATCAGATACACGGAAAACTTCATCTAATTTATGGGAAATAAAAATAATCGCGATGCCTCTTTCTTTTACCGTTTCAATAATGCGGTAAAGCTGCTCGACCTCATTTTCCGTCAATGCCGTGGTCGGCTCGTCCATAATGATGACTTTGGCGTCATTAGAAACCGCCGTAGCAATAGCAACTAACTGCGCTTTTGCCACCGATACATCGGACATTTTGGCGGTCACGTCCATCGGCACGCCAAGCTCTTTTAATATCGCCGAAGCCTGCTCATCCATTGCTTTCTGATCGAGAAAAAACTTGGCTTGGGTAATTTCACGCCCTAAGTAAAAATTATCGGCGATGGTCAAATTGGCAGCCGGGGAAATTTCCTGCGGCACCATGGTTAAGCCGCAACGAATCGCATAAATAGGACGACTTGGTTTAAAGGGTTTGCCGTCCAAAATTAATTCTCCGCCAT  >  1/1958693‑1959165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑