Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,952,916	C→T	C584C (TGC→TGT)	unknown →	Iron siderophore receptor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,952,916	0	C	T	100.0%	220.9 / NA	73	C584C (TGC→TGT)	unknown	Iron siderophore receptor protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/43);  total (30/43)

CTATCCTGACAATGCTGTAGCACCGTTTTACATCAGAAACGCAAAGCGCAAAGAAAAAAGTGCCTTCATTTCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGCCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGCACACAAATAGTCGATTATCAAACCTACAAGTTAAAGCCTGAGAGACAAAAGAACTGGGAAATTGGCACAAATCTATTAT  >  1/1952670‑1953148                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         cTATCCTGACAATGCTGTAGCACCGTTTTACATCAGAAACGCAAAGCGCAAAGAAAAAAGTGCCTTCATTTCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCgg                                                                                                                                                                                                                                       <  1:876468/250‑1 (MQ=255)   aTCCTGACAATGCTGTAGCACCGTTTTACATCAGAAACGCAAAGCGCAAAGAAAAAAGTGCCTTCATTTCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGAtt                                                                                                                                                                                                                                    >  1:515941/1‑251 (MQ=255)       tGACAATGCTGTAGCACCGTTTTACATCAGAAACGCAAAGCGCAAAGAAAAAAGTGCCTTCATTTCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTAtt                                                                                                                                                                                                                                >  1:673325/1‑251 (MQ=255)         aCAATGCTGTAGCACCGTTTTACATCAGAAACGCAAAGCGCAAAGAAAAAAGTGCCTTCATTTCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTc                                                                                                                                                                                                                               <  1:515383/250‑1 (MQ=255)            aTGCTGTAGCACCGTTTTACATCAGAAACGCAAAGCGCAAAGAAAAAAGTGCCTTCATTTCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTgg                                                                                                                                                                                                                           >  1:381413/1‑251 (MQ=255)                          ttttACATCAGAAACGCAAAGCGCAAAGAAAAAAGTGCCTTCATTTCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAg                                                                                                                                                                                                             >  1:624306/1‑251 (MQ=255)                          ttttACATCAGAAACGCAAAGCGCAAAGAAAAAAGTGCCTTCATTTCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAg                                                                                                                                                                                                             <  1:385801/251‑1 (MQ=255)                                     aaaCGCAAAGCGCAAAGAAAAAAGTGCCTTCATTTCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGc                                                                                                                                                                                                    <  1:335441/249‑1 (MQ=255)                                      aaCGCAAAGCGCAAAGAAAAAAGTGCCTTCATTTCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCcac                                                                                                                                                                                                 <  1:236815/251‑1 (MQ=255)                                            aagcgcaaagAAAAAAGTGCCTTCATTTCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCAcc                                                                                                                                                                                           <  1:273847/251‑1 (MQ=255)                                                 caaagAAAAAAGTGCCTTCATTTCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATcg                                                                                                                                                                                      <  1:488075/251‑1 (MQ=255)                                                              gCCTTCATTTCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTTAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGc                                                                                                                                                                                                    >  1:486580/1‑224 (MQ=255)                                                                   cATTTCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAAc                                                                                                                                                                    <  1:772517/251‑1 (MQ=255)                                                                       tCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACcaca                                                                                                                                                                >  1:736951/1‑251 (MQ=255)                                                                       tCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACcaca                                                                                                                                                                >  1:231862/1‑251 (MQ=255)                                                                           ttAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATgag                                                                                                                                                            <  1:781988/251‑1 (MQ=255)                                                                             aaCTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGaa                                                                                                                                                          <  1:371737/251‑1 (MQ=255)                                                                              aCTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAAc                                                                                                                                                         <  1:374038/251‑1 (MQ=255)                                                                                 aTCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAAcgcg                                                                                                                                                      >  1:615847/1‑251 (MQ=255)                                                                                 aTCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAAcgcg                                                                                                                                                      >  1:504174/1‑251 (MQ=255)                                                                                      aTCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGt                                                                                                                                                                                <  1:370466/220‑1 (MQ=255)                                                                                      aTCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATgag                                                                                                                                                            >  1:294364/1‑240 (MQ=255)                                                                                      aTCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAAt                                                                                                                                                 >  1:718579/1‑251 (MQ=255)                                                                                                ggCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATaaaa                                                                                                                                       >  1:329998/1‑251 (MQ=255)                                                                                                 gCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAAc                                                                                                                                                         >  1:471809/1‑232 (MQ=255)                                                                                                      aaaTAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAAt                                                                                                                                                                                        <  1:60367/196‑1 (MQ=255)                                                                                                      aaaTAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGaa                                                                                                                                                          <  1:534823/226‑1 (MQ=255)                                                                                                          aGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAgg                                                                                                                             >  1:822085/1‑251 (MQ=255)                                                                                                           gATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCg                                                                                                                                                                                                           <  1:275577/172‑1 (MQ=255)                                                                                                           gATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAat                                                                                                                                      >  1:63689/1‑241 (MQ=255)                                                                                                           gATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAgg                                                                                                                             >  1:409019/1‑250 (MQ=255)                                                                                                            aTCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCcac                                                                                                                                                                                                 <  1:398627/181‑1 (MQ=255)                                                                                                            aTCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATgag                                                                                                                                                            <  1:396926/218‑1 (MQ=255)                                                                                                            aTCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAgg                                                                                                                             >  1:602980/1‑249 (MQ=255)                                                                                                                  gTCTGCGACGCATCTGCACCGCAATGCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACg                                                                                                                      <  1:471950/250‑1 (MQ=255)                                                                                                                  gTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTg                                                                                                                                                                                                                            <  1:197264/148‑1 (MQ=255)                                                                                                                  gTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGc                                                                                                                                                                                                                          <  1:680919/150‑1 (MQ=255)                                                                                                                  gTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAAc                                                                                                                                                         <  1:803708/215‑1 (MQ=255)                                                                                                                  gTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTAc                                                                                                                       <  1:407267/249‑1 (MQ=255)                                                                                                                   tCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGc                                                                                                                     <  1:189076/250‑1 (MQ=255)                                                                                                                                 gcaccgcaATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCtt                                                                                                                                                                         <  1:684915/184‑1 (MQ=255)                                                                                                                                 gcaccgcaATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAgtttgttt                                                                                                      >  1:58719/1‑251 (MQ=255)                                                                                                                                             aTGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCAcgc                                                                                          <  1:202545/251‑1 (MQ=255)                                                                                                                                                 ttttCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGAt                                                                                      <  1:618494/251‑1 (MQ=255)                                                                                                                                                    tCAGCCCAGAGTCGAAGATAAATTCCGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGAttt                                                                                    <  1:629953/251‑1 (MQ=255)                                                                                                                                                        cccAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGcac                                                                               <  1:92654/251‑1 (MQ=255)                                                                                                                                                        cccAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGca                                                                                <  1:754928/250‑1 (MQ=255)                                                                                                                                                        cccAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGc                                                                                 <  1:15771/249‑1 (MQ=255)                                                                                                                                                        cccAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGc                                                                                 <  1:718324/249‑1 (MQ=255)                                                                                                                                                            gagTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGCACACaa                                                                           >  1:569666/1‑251 (MQ=255)                                                                                                                                                             agTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGcacac                                                                             <  1:345518/248‑1 (MQ=255)                                                                                                                                                              gTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGc                                                                                                                                                                                                    <  1:744335/128‑1 (MQ=255)                                                                                                                                                              gTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGCACACAAAt                                                                         >  1:678930/1‑251 (MQ=255)                                                                                                                                                                     aTAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGc                                                                                                                                                                                                    >  1:326395/1‑121 (MQ=255)                                                                                                                                                                                   cAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACAt                                                                                                                                           >  1:661556/1‑165 (MQ=255)                                                                                                                                                                                      aaaTATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGAt                                                                                                                                                                                  <  1:396396/123‑1 (MQ=255)                                                                                                                                                                                      aaaTATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGca                                                                                <  1:464393/221‑1 (MQ=255)                                                                                                                                                                                      aaaTATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGCACACAAATAGTCGATTATCAAACCTACAAGt                                                  >  1:16786/1‑251 (MQ=255)                                                                                                                                                                                      aaaTATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGCACACAAATAGTCGATTATCAAACCTACAAGt                                                  >  1:67089/1‑251 (MQ=255)                                                                                                                                                                                                 aTCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCtt                                                                                                                                                                         <  1:456290/121‑1 (MQ=255)                                                                                                                                                                                                 aTCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGCACACAAATAGTCGATTATCAAACCTACAAGTTAAAGCCTgag                                       >  1:81063/1‑251 (MQ=255)                                                                                                                                                                                                   ccGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAAc                                                                                                                                                                    <  1:284126/124‑1 (MQ=255)                                                                                                                                                                                                     gaagaaTTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGca                                                                                <  1:792523/206‑1 (MQ=255)                                                                                                                                                                                                                tCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAgg                                                                                                                             >  1:119803/1‑150 (MQ=255)                                                                                                                                                                                                                tCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGAttt                                                                                    <  1:437898/191‑1 (MQ=255)                                                                                                                                                                                                                tCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGCACACAAATAGTCGATTATCAAACCTACAAGTTAAAGCCTGAGAGACAAAAGAACTgg                        >  1:343016/1‑251 (MQ=255)                                                                                                                                                                                                                 cTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGcac                                                                               >  1:595796/1‑195 (MQ=255)                                                                                                                                                                                                                 cTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGCACACAAATAGTCGATTATCAAACCTACAAGTTAAAGCCTGAGAGACAAAAGAACTggg                       >  1:418711/1‑251 (MQ=255)                                                                                                                                                                                                                           gTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATgag                                                                                                                                                            <  1:825042/108‑1 (MQ=255)                                                                                                                                                                                                                           gTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAat                                                                                                                                      <  1:321297/130‑1 (MQ=255)                                                                                                                                                                                                                           gTGCGCAACAACCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGCACACAAATAGTCGATTATCAAACCTACAAGTTAAAGCCTGAGAGACAAAAGAACTGGGAAATTGGca              >  1:400874/1‑250 (MQ=255)                                                                                                                                                                                                                                caacaaCCCGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGCACACAAATAGTCGATTATCAAACCTACAAGTTAAAGCCTGAGAGACAAAAGAACTGGGAAATTGGCACAAATc        <  1:63434/251‑1 (MQ=255)                                                                                                                                                                                                                                      cccGTTTCAAAAAAGATGTCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGCACACAAATAGTCGATTATCAAACCTACAAGTTAAAGCCTGAGAGACAAAAGAACTGGGAAATTGGCACAAATCtattat  <  1:723171/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                         CTATCCTGACAATGCTGTAGCACCGTTTTACATCAGAAACGCAAAGCGCAAAGAAAAAAGTGCCTTCATTTCGCTTAACTATCCAATCACGAACTGGCTTAAAATAGATCTAGGTCTGCGACACATCCGCACCGCAATTCATGATTTTCAGCCCAGAGTCGAAGATAAATT‑CGTACGCAAAAATATGCCCGATCCGAAGAATTCCGTCTGTGAACCGGTGCGCAACAACCCGTTTCAAAAAAGATGCCGGATTTATTCTTGGCCGCAACCTCCAAGCGTTAAGGCCACATCACCAATCGCGATGTTTACCTTCGAACCACATGAGAACGCGCAAATTTACATAAAATATGCCGAGGCTGTACGCTCAGCGAGTTTGTTTCAAGCATCACGCGGATTTAGCACACAAATAGTCGATTATCAAACCTACAAGTTAAAGCCTGAGAGACAAAAGAACTGGGAAATTGGCACAAATCTATTAT  >  1/1952670‑1953148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑