Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,947,404	A→G	A355A (GCA→GCG)	unknown →	Queuine tRNA‑ribosyltransferase (EC 2.4.2.29)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,947,404	0	A	G	100.0%	224.1 / NA	79	A355A (GCA→GCG)	unknown	Queuine tRNA‑ribosyltransferase (EC 2.4.2.29)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/41);  total (38/41)

ACCGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAGACGACACCAGCCCGCCGGATCCGCACTGTGACTGTTACACCTGCAAACACTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCAGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTAAAAATCATCAAATCTGCTAAATAAACAAATTTGCTCAATGCACAAAATCGTAG  >  1/1947189‑1947648                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     cccGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAGACGACACCAGCCCGCCGGATCCGCACTGTGACTGTTACACCTGCAAACACTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGc                                                                                                                                                                                                                   <  1:758376/250‑1 (MQ=255)                                 aTACAAAGACGACACCAGCCCGCCGGATCCGCACTGTGACTGTTACACCTGCAAACACTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAAt                                                                                                                                                                                   >  1:340020/1‑251 (MQ=255)                                 aTACAAAGACGACACCAGCCCGCCGGATCCGCACTGTGACTGTTACACCTGCAAACACTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAAt                                                                                                                                                                                   >  1:494101/1‑251 (MQ=255)                                  tACAAAGACGACACCAGCCCGCCGGATCCGCACTGTGACTGTTACACCTGCAAACACTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTtgatg                                                                                                                                                                                                                                         <  1:821582/196‑1 (MQ=255)                                          cgacACCAGCCCGCCGGATCCGCACTGTGACTGTTACACCTGCAAACACTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAg                                                                                                                                                                          <  1:462220/251‑1 (MQ=255)                                                            tCCGCACTGTGACTGTTACACCTGCAAACACTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAAtt                                                                                                                                                                                  <  1:886343/225‑1 (MQ=255)                                                            tCCGCACTGTGACTGTTACACCTGCAAACACTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGaaa                                                                                                                                                        >  1:785601/1‑251 (MQ=255)                                                                    gtgACTGTTACACCTGCAAACACTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAAtt                                                                                                                                                                                  >  1:872019/1‑217 (MQ=255)                                                                        cTGTTACACCTGCAAACACTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATcc                                                                                                                                                            <  1:785775/235‑1 (MQ=255)                                                                                ccTGCAAACACTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAg                                                                                                                                                      <  1:782105/233‑1 (MQ=255)                                                                                ccTGCAAACACTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTaaaaaa                                                                                                                                    >  1:500579/1‑251 (MQ=255)                                                                                  tGCAAACACTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACg                                                                                                                                  >  1:656968/1‑251 (MQ=255)                                                                                        cactctccccaaGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAAtt                                                                                                                                                                                  <  1:701899/187‑1 (MQ=255)                                                                                        cacTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGtttttt                                                                                                                            >  1:193819/1‑251 (MQ=255)                                                                                        cacTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGtttttt                                                                                                                            >  1:412286/1‑251 (MQ=255)                                                                                          cTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAAtt                                                                                                                                                                                  <  1:486191/195‑1 (MQ=255)                                                                                          cTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTaa                                                                                                                          >  1:629064/1‑251 (MQ=255)                                                                                            ataCCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAAt                                                                                                                        <  1:803676/251‑1 (MQ=255)                                                                                            ataCCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAAt                                                                                                                        <  1:399131/251‑1 (MQ=255)                                                                                            ataCCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAAt                                                                                                                        >  1:109788/1‑251 (MQ=255)                                                                                              aCCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCGATTTAAAAAACGTTTTTTaaaatg                                                                                                                      <  1:542350/251‑4 (MQ=255)                                                                                                        aTTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCa                                                                                                                                              >  1:862815/1‑217 (MQ=255)                                                                                                          ttGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTtc                                                                                                          <  1:46120/251‑1 (MQ=255)                                                                                                           tGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACtttt                                                                                                           >  1:153913/1‑249 (MQ=255)                                                                                                            gTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTtct                                                                                                         >  1:611561/1‑250 (MQ=255)                                                                                                            gTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTtct                                                                                                         >  1:454192/1‑250 (MQ=255)                                                                                                               tCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAAc                                                                                                     <  1:667988/251‑1 (MQ=255)                                                                                                                cACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACg                                                                                                    >  1:684472/1‑251 (MQ=255)                                                                                                                cACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACg                                                                                                    >  1:520201/1‑251 (MQ=255)                                                                                                                     ggccAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCTTTATTCCCACCGCTTAATGGCAGAAATTCGCCAGGTGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAATCCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAAc                                                                                                     <  1:615212/242‑1 (MQ=255)                                                                                                                        cAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAaac                                                                                            <  1:253534/251‑1 (MQ=255)                                                                                                                            tgtgGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAAcc                                                                                        <  1:795193/251‑1 (MQ=255)                                                                                                                               ggCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAg                                                                                                                                                      >  1:462454/1‑186 (MQ=255)                                                                                                                                gCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACcaca                                                                                     <  1:584134/250‑1 (MQ=255)                                                                                                                                 cGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTtcggattactgatttgcttctgct                                                                                    <  1:247172/250‑23 (MQ=39)                                                                                                                                      ttCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCttttt                                                                              <  1:188734/251‑1 (MQ=255)                                                                                                                                            gTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTAc                                                                         <  1:321777/250‑1 (MQ=255)                                                                                                                                            gTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTAc                                                                         <  1:877778/250‑1 (MQ=255)                                                                                                                                                 cGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGAt                                                                    >  1:654428/1‑250 (MQ=255)                                                                                                                                                   ccTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATc                                                                                                                                                                    >  1:467642/1‑152 (MQ=255)                                                                                                                                                   ccTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTaa                                                                                                                                                               >  1:156274/1‑157 (MQ=255)                                                                                                                                                   ccTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCAc                                                                                                               >  1:770720/1‑205 (MQ=255)                                                                                                                                                   ccTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTtctc                                                                                                        <  1:376069/212‑1 (MQ=255)                                                                                                                                                   ccTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAAcc                                                                                        <  1:897774/228‑1 (MQ=255)                                                                                                                                                   ccTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTAc                                                                         <  1:579352/243‑1 (MQ=255)                                                                                                                                                   ccTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGAt                                                                    >  1:245646/1‑248 (MQ=255)                                                                                                                                                   ccTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGAt                                                                    >  1:343798/1‑248 (MQ=255)                                                                                                                                                   ccTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGAt                                                                    >  1:683137/1‑248 (MQ=255)                                                                                                                                                   ccTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGAt                                                                    >  1:253834/1‑248 (MQ=255)                                                                                                                                                   ccTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGAt                                                                    >  1:602153/1‑248 (MQ=255)                                                                                                                                                   ccTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGAt                                                                    >  1:297959/1‑248 (MQ=255)                                                                                                                                                   ccTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGAt                                                                    >  1:34467/1‑248 (MQ=255)                                                                                                                                                   ccTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGAt                                                                    >  1:902104/1‑248 (MQ=255)                                                                                                                                                   ccTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCt                                                                  <  1:457641/250‑1 (MQ=255)                                                                                                                                                   ccTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCAGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGAt                                                                    >  1:426416/1‑248 (MQ=255)                                                                                                                                                      tAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTgaag                                                              <  1:610453/251‑1 (MQ=255)                                                                                                                                                        aTACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTgaagaa                                                            >  1:497557/1‑251 (MQ=255)                                                                                                                                                         tACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGaagaag                                                           <  1:763375/251‑1 (MQ=255)                                                                                                                                                          aCCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGaagaag                                                           >  1:572851/1‑250 (MQ=255)                                                                                                                                                                 acaATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTaaaaa                                                   <  1:398603/251‑1 (MQ=255)                                                                                                                                                                 acaATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCAAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTaaaaa                                                   <  1:843544/251‑1 (MQ=255)                                                                                                                                                                  caATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTAAAAat                                                  <  1:331829/251‑1 (MQ=255)                                                                                                                                                                        gCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAaac                                                                                            >  1:177504/1‑203 (MQ=255)                                                                                                                                                                        gCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTAAAAATCATCaa                                            >  1:617588/1‑251 (MQ=255)                                                                                                                                                                           ttattaCCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTAAAAATCATCAAATc                                         <  1:128623/251‑1 (MQ=255)                                                                                                                                                                             attaCCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGTAGCAAATCAGTAATCCGaaa                                                                                                                                                        <  1:889173/138‑1 (MQ=255)                                                                                                                                                                             attaCCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCa                                                                                                       <  1:483589/187‑1 (MQ=255)                                                                                                                                                                               taCCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTAAAAATCATCAAATCTg                                       >  1:99001/1‑249 (MQ=255)                                                                                                                                                                                aCCAACGCTTAATGGCAGAAATTCGCGAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTAAAAATCATCAAATCTGCta                                    <  1:334759/251‑1 (MQ=255)                                                                                                                                                                                aCCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTAAAAATCATCAAATCTGCta                                    <  1:688459/251‑1 (MQ=255)                                                                                                                                                                                    aCGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTAAAAATCATCAAATCTGCtaaata                                >  1:58211/1‑251 (MQ=255)                                                                                                                                                                                     cGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAGACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTAAAAATCATCAAATCTGCtaaataa                               <  1:306238/251‑1 (MQ=255)                                                                                                                                                                                     cGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTAAAAATCATCAAATCTGCtaaataa                               >  1:787724/1‑251 (MQ=255)                                                                                                                                                                                              gCAGAAATTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAg                                                                                                                                                      <  1:350705/123‑1 (MQ=255)                                                                                                                                                                                                  aaaTTCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTAAAAATCATCAAATCTGCTAAATAAACAAATTTGCTc                   >  1:280659/1‑250 (MQ=255)                                                                                                                                                                                                      tCGCCAGGCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTAAAAATCATCAAATCTGCTAAATAAACAAATTTGCTCAATGc              <  1:699360/251‑1 (MQ=255)                                                                                                                                                                                                            ggCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTccgcc                                                                                                                                                                                           <  1:885987/72‑1 (MQ=255)                                                                                                                                                                                                             gCGATCGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTAAAAATCATCAAATCTGCTAAATAAACAAATTTGCTCAATGCACaaa         <  1:70018/249‑1 (MQ=255)                                                                                                                                                                                                                  cGAAGCGGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTAAAAATCATCAAATCTGCTAAATAAACAAATTTGCTCAATGCACAAAATCGTAg  <  1:281488/251‑1 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     ACCGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAGACGACACCAGCCCGCCGGATCCGCACTGTGACTGTTACACCTGCAAACACTATACCAAAGCCTATTTGTATCACCTGGACAAATGTGGCGAAATTCTTGGTGCACGCCTTAATACCATCCACAATTTGCGTTATTACCAACGCTTAATGGCAGAAATTCGCCAGGCGATCGAAGCAGATCGTTTTGATGATTTCGTGGTGGAATTTTATGCCAGAATCGGCAAACCTGTTCCGCCGTTGCAATTAGCAGAAGCAAATCAGTAATCCGAAAAGTGCGGTCATTTTAAAAAACGTTTTTTAAATCGACCGCACTTTTCTCAACGACAGAAACAACCACATCTTTTTTCTACCGGATCTGAAGAAGCTTAAAAATCATCAAATCTGCTAAATAAACAAATTTGCTCAATGCACAAAATCGTAG  >  1/1947189‑1947648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑