| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,947,028 | G→A | R230H (CGC→CAC) | unknown → | Queuine tRNA‑ribosyltransferase (EC 2.4.2.29) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,947,028 | 0 | G | A | 98.8% | 219.2 / ‑5.9 | 81 | R230H (CGC→CAC) | unknown | Queuine tRNA‑ribosyltransferase (EC 2.4.2.29) |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (35/45); minor base C (0/1); total (35/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
AATGTACGCCCTACCCCGCCACGTTTAATTATGCCAAGCAATCCATGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCGCATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAGACGACACCAGCCCGCCGGATCCGCACTGTGACTGTTACAC > 1/1946782‑1947268 | aaTGTACGCCCTACCCCGCCACGTTTAATTATGCCAAGCAATCCATGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACAtt > 1:891350/1‑251 (MQ=255) aTGTACGCCCTACCCCGCCACGTTTAATTATGCCAAGCAATCCATGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACAttt > 1:99784/1‑251 (MQ=255) aTGTACGCCCTACCCCGCCACGTTTAATTATGCCAAGCAATCCATGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACAttt > 1:882535/1‑251 (MQ=255) aTGTACGCCCTACCCCGCCACGTTTAATTATGCCAAGCAATCCATGGAAACGTCCCTGCGCTGGCCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAACGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGCGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACAttt > 1:99388/1‑251 (MQ=255) tACGCCCTACCCCGCCACGTTTAATTATGCCAAGCAATCCATGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTgg < 1:36835/251‑1 (MQ=255) cGCCCTACCCCGCCACGTTTAATTATGCCAAGCAATCCATGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAg < 1:587187/251‑1 (MQ=255) cctcccccGCCACGTTTAATTATGCCAAGCAATCCATGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTAc < 1:115737/247‑1 (MQ=255) aCCCCGCCACGTTTAATTATGCCAAGCAATCCATGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTAGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATc < 1:738641/251‑1 (MQ=255) tAATTATGCCAAGCAATCCATGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGAtt > 1:107425/1‑250 (MQ=255) tAATTATGCCAAGCAATCCATGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGAtt > 1:329468/1‑250 (MQ=255) aTGCCAAGCAATCCATGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAg < 1:730110/227‑1 (MQ=255) ccAAGCAATCCATGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGAc < 1:825037/251‑1 (MQ=255) aTCCATGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTgg < 1:807346/215‑1 (MQ=255) aTCCATGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACaa < 1:178061/246‑1 (MQ=255) ccATGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACcgcg > 1:590782/1‑250 (MQ=255) tGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTACCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTAt < 1:445154/251‑1 (MQ=255) gAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACcg > 1:40540/1‑243 (MQ=255) aaaTGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTAttt < 1:658782/250‑1 (MQ=255) gTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATgg > 1:96633/1‑251 (MQ=255) ctCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCAACCCAAAAGAAGATATGCACCCCATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATgg < 1:151155/250‑1 (MQ=255) tCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATggg < 1:654107/251‑1 (MQ=255) tCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATggg < 1:37814/251‑1 (MQ=255) tCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATggg < 1:731337/251‑1 (MQ=255) tCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATgg < 1:806638/250‑1 (MQ=255) gcTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATc > 1:502453/1‑203 (MQ=255) cTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGaa < 1:735908/251‑1 (MQ=255) cTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGaa < 1:531158/251‑1 (MQ=255) cTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGa < 1:66596/250‑1 (MQ=255) gCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTa > 1:667753/1‑251 (MQ=255) cGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAgg > 1:645799/1‑251 (MQ=255) gAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATggg < 1:880244/212‑1 (MQ=255) gAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTcg > 1:741918/1‑251 (MQ=255) ttAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCgg > 1:523908/1‑251 (MQ=255) accaaAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGa < 1:770964/249‑1 (MQ=255) caaAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGata < 1:756184/251‑1 (MQ=255) aaCGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGtt < 1:667916/251‑1 (MQ=255) cTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTg < 1:840122/251‑1 (MQ=255) gTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAg < 1:123521/142‑1 (MQ=255) aTCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATgg > 1:165838/1‑178 (MQ=255) aTCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCaa > 1:564218/1‑250 (MQ=255) cGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATg < 1:407740/251‑1 (MQ=255) ccTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGata > 1:92749/1‑216 (MQ=255) ttGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTgg > 1:116705/1‑114 (MQ=255) aagaaTTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATggg < 1:55347/251‑1 (MQ=255) aagaaTTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATggg < 1:427528/251‑1 (MQ=255) aaTTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCa < 1:610244/250‑1 (MQ=255) gcgcAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTAt > 1:824632/1‑251 (MQ=255) gTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTgg < 1:485037/97‑1 (MQ=255) cACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGAc < 1:609483/251‑1 (MQ=255) cTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTAt < 1:264228/132‑1 (MQ=255) ggCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATc < 1:846741/93‑1 (MQ=255) ggCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGAt > 1:349217/1‑153 (MQ=255) ggCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATg > 1:252457/1‑214 (MQ=255) aaTCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAAcc < 1:482449/202‑1 (MQ=255) aTCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAAt > 1:21808/1‑251 (MQ=255) aTCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATCGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAAt > 1:312059/1‑251 (MQ=255) cAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGata < 1:509546/176‑1 (MQ=255) cAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGAc > 1:324979/1‑235 (MQ=255) atatCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATgg < 1:479410/123‑1 (MQ=255) atatCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGAt > 1:768399/1‑182 (MQ=255) gCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGatat < 1:861861/160‑1 (MQ=255) gCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACaaa > 1:871681/1‑251 (MQ=255) gCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACaaa > 1:297878/1‑251 (MQ=255) gCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACaaa > 1:858401/1‑251 (MQ=255) gTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATg < 1:363388/181‑1 (MQ=255) gTGGGCGGTTTAGCCGTGGGCAAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATgg < 1:742621/100‑1 (MQ=255) cGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAGACGACACCAGcc < 1:738344/251‑1 (MQ=255) gTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAGACGACACCAGCccg > 1:896851/1‑251 (MQ=255) gTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAGACGACACCAGCccg > 1:856590/1‑251 (MQ=255) gTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAGACGACACCAGCccg > 1:839550/1‑251 (MQ=255) gTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAGACGACACCAGCccg > 1:28407/1‑251 (MQ=255) gCCGTGGGCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAGACGACACCAGCCCGCCGGa > 1:581171/1‑251 (MQ=255) gggCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATc < 1:594545/208‑1 (MQ=255) ggCGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAGACGACACCAGCCCGCCGGATCCGCa > 1:542073/1‑251 (MQ=255) cGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAGACGACACCAGCCCGCCGGATCCGCAc < 1:365683/250‑1 (MQ=255) cGAACCAAAAGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAGACGACACCAGCCCGCCGGATCCGCAc < 1:71524/250‑1 (MQ=255) aaaGAAGATATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATg < 1:110125/163‑1 (MQ=255) gaagaTATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCAt > 1:335171/1‑116 (MQ=255) aagaTATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAga < 1:387813/214‑1 (MQ=255) aagaTATGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAGACGACACCAGCCCGCCGGATCCGCACTGTGACTGtt < 1:471075/249‑1 (MQ=255) atatGCACCACATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAGACGACACCAGCCCGCCGGATCCGCACTGTGACTGTCacac < 1:707273/250‑1 (MQ=255) | AATGTACGCCCTACCCCGCCACGTTTAATTATGCCAAGCAATCCATGGAAATGTCCCTGCGCTGGGCGCAACGCAGCCGCGCTCGTTTTGACGAATTAGGCAACAAAAACGCGCTGTTCGGCATTATCCAAGGCGGAACCTTTGAAGAATTGCGCAAAGTTTCACTGGAAGGCTTAATCAATATCGGCTTTGACGGCTATGCGGTGGGCGGTTTAGCCGTGGGCGAACCAAAAGAAGATATGCACCGCATTTTGGAGTACATCTGCCCGCAGATTCCCGCCGACAAACCGCGCTATTTAATGGGTGTAGGAAAACCGGAAGATTTAGTGGAAGGCGTACGTCGCGGCATTGATATGTTCGATTGCGTGATGCCAACCCGCAATGCGCGTAATGGGCATTTATTCGTCACCGACGGCATCGTTAAAATCCGTAATGCCAAATACAAAGACGACACCAGCCCGCCGGATCCGCACTGTGACTGTTACAC > 1/1946782‑1947268 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |