Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,945,451	T→C	P139P (CCA→CCG)	unknown ←	Periplasmic aromatic amino acid aminotransferase beta precursor (EC 2.6.1.57)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,945,451	0	T	C	100.0%	232.1 / NA	81	P139P (CCA→CCG)	unknown	Periplasmic aromatic amino acid aminotransferase beta precursor (EC 2.6.1.57)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/44);  total (37/44)

CCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCCAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACTGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTATATTTTTCAGCTAATTGACGGATTAATTCACTACGATGCTCATCGGGATAACGGGAACCAATCCCAATGC  >  1/1945215‑1945692                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  ccACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGAAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGc                                                                                                                                                                                                                                     <  1:288219/251‑1 (MQ=255)  cACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCt                                                                                                                                                                                                                                    <  1:743307/251‑1 (MQ=255)  cACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCt                                                                                                                                                                                                                                    <  1:61852/251‑1 (MQ=255)  cACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGAAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCt                                                                                                                                                                                                                                    <  1:688133/251‑1 (MQ=255)    cTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTg                                                                                                                                                                                                                                  >  1:24469/1‑251 (MQ=255)      ttCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTgcg                                                                                                                                                                                                                                >  1:163098/1‑251 (MQ=255)                ggATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCt                                                                                                                                                                                                                                    >  1:894174/1‑237 (MQ=255)                  aTCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGTTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTACCGGTTGAAGTTGGATTATTGGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCt                                                                                                                                                                                                                                    <  1:28428/235‑1 (MQ=255)                  aTCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCt                                                                                                                                                                                                                                    >  1:138011/1‑235 (MQ=255)                  aTCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCAtata                                                                                                                                                                                                                          >  1:2684/1‑245 (MQ=255)                  aTCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCgg                                                                                                                                                                                                                    >  1:400519/1‑251 (MQ=255)                  aTCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCgg                                                                                                                                                                                                                    >  1:164655/1‑251 (MQ=255)                       gTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCt                                                                                                                                                                                                                                    <  1:213206/230‑1 (MQ=255)                       gTACATATTCGGCGTAGGCTTCAACTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCATAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGc                                                                                                                                                                                                                                     <  1:487254/229‑1 (MQ=255)                          cATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTa                                                                                                                                                                                                            <  1:73823/251‑1 (MQ=255)                                 ggCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCg                                                                                                                                                                                                     >  1:568882/1‑251 (MQ=255)                                     aaggtttCATCTAGTAAAAAGAAATAATTTTCCGGGGCTTTTTTAATCCAACGGAATAACTCTTTTGCGGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAAAAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATc                                                                                                                                                                                                 <  1:152089/246‑1 (MQ=255)                                       ggCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTa                                                                                                                                                                                                            >  1:838935/1‑238 (MQ=255)                                            cATCTAGTAAAAAGAAATAATTTTCCGGCGTTTTTTTAATCCAACGGAATACCTCTTTTGCCGGCGTAACGTTTGAATTTGGATTATTCGGGTTACACAAAAAAAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGcacc                                                                                                                                                                                          <  1:190680/251‑1 (MQ=255)                                              tctaGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGcaccac                                                                                                                                                                                        <  1:748437/248‑1 (MQ=255)                                                       aaGAAATAATTTTCCGGCGCTTTTTTAAACCAAGGGAATAGCTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTGGGTTTACACAAATAGAAAATACTCACGCCACTAAGCTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAAc                                                                                                                                                                               <  1:41337/251‑1 (MQ=255)                                                          aaaTAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCg                                                                                                                                                                             >  1:214755/1‑250 (MQ=255)                                                             ccttctttcGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTg                                                                                                                                                                         <  1:796955/243‑1 (MQ=255)                                                                ttttCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGttt                                                                                                                                                                      <  1:506061/251‑1 (MQ=255)                                                                tgttCCGGCGCTTTTTAAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGttt                                                                                                                                                                      <  1:486938/249‑1 (MQ=255)                                                                             ttttAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCt                                                                                                                                                                                                                                    >  1:575503/1‑176 (MQ=255)                                                                                  aTCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTaaa                                                                                                                                                    <  1:381229/251‑1 (MQ=255)                                                                                      aaCGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCt                                                                                                                                                                                                                                    >  1:763293/1‑167 (MQ=255)                                                                                          gAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCtgtg                                                                                                                                            >  1:622747/1‑251 (MQ=255)                                                                                            aTAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGAc                                                                                                                                          <  1:101152/251‑1 (MQ=255)                                                                                                 cctTTGGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGaat                                                                                                                                     <  1:532006/250‑1 (MQ=255)                                                                                                      tGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTc                                                                                                                                <  1:570011/251‑1 (MQ=255)                                                                                                                   gTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATgg                                                                                                                                                                                                                                      <  1:30718/136‑1 (MQ=255)                                                                                                                       aaGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCagag                                                                                                               >  1:278475/1‑251 (MQ=255)                                                                                                                            ggATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTaaa                                                                                                                                                    >  1:475381/1‑209 (MQ=255)                                                                                                                                 attCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTc                                                                                                      >  1:20276/1‑250 (MQ=255)                                                                                                                                  ttCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTccc                                                                                                    <  1:807763/251‑1 (MQ=255)                                                                                                                                   tCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATTTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTcccc                                                                                                   <  1:446667/251‑1 (MQ=255)                                                                                                                                     gggTTACAAAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTccc                                                                                                    <  1:504821/248‑1 (MQ=255)                                                                                                                                      ggTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAg                                                                                                >  1:609736/1‑251 (MQ=255)                                                                                                                                        ttACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGa                                                                                              <  1:94456/251‑1 (MQ=255)                                                                                                                                          acacAAATAGAAAATCCTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCtg                                                                                                                                              <  1:89048/201‑1 (MQ=255)                                                                                                                                             caAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAAtt                                                                                                                                                                                                                       <  1:455698/125‑1 (MQ=255)                                                                                                                                             caAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATAcc                                                                                                                                                >  1:786275/1‑196 (MQ=255)                                                                                                                                                aTAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAAt                                                                                      >  1:830916/1‑251 (MQ=255)                                                                                                                                                 tAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGAc                                                                                                                                          >  1:610284/1‑198 (MQ=255)                                                                                                                                                 tAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAAtt                                                                                     >  1:420908/1‑251 (MQ=255)                                                                                                                                                   gAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCtg                                                                                                                                              <  1:864504/192‑1 (MQ=255)                                                                                                                                                   gAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAAt                                                                                      <  1:121564/248‑1 (MQ=255)                                                                                                                                                    aaaaTCCTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGAc                                                                                                                                          <  1:128812/195‑1 (MQ=255)                                                                                                                                                     aaaTACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCt                                                                                 <  1:382881/251‑1 (MQ=255)                                                                                                                                                       aTACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCtt                                                                                >  1:323284/1‑250 (MQ=255)                                                                                                                                                         aCTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCt                                                                                 >  1:187234/1‑247 (MQ=255)                                                                                                                                                              cGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAg                                                                            >  1:229535/1‑247 (MQ=255)                                                                                                                                                                ccACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGAc                                                                                                                                          >  1:87111/1‑183 (MQ=255)                                                                                                                                                                ccACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTccc                                                                                                    <  1:551393/221‑1 (MQ=255)                                                                                                                                                                ccACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATtata                                                                      >  1:391022/1‑251 (MQ=255)                                                                                                                                                                ccACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATtata                                                                      >  1:851226/1‑251 (MQ=255)                                                                                                                                                                ccACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAAACTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATtata                                                                      >  1:90937/1‑251 (MQ=255)                                                                                                                                                                ccACTAAACTGCTCGGCGGCTTCTTGTAATTTTGCCAATTCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCt                                                                                                                                                                                                                                    >  1:674638/1‑93 (MQ=255)                                                                                                                                                                 cACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTatat                                                                     <  1:879801/251‑1 (MQ=255)                                                                                                                                                                 cACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTatat                                                                     <  1:640585/251‑1 (MQ=255)                                                                                                                                                                 cACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTatat                                                                     >  1:159608/1‑251 (MQ=255)                                                                                                                                                                 cACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTatat                                                                     <  1:57867/251‑1 (MQ=255)                                                                                                                                                                      aaCTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTATATTTTTc                                                                <  1:127006/251‑1 (MQ=255)                                                                                                                                                                                  gTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGAtt                                                                         >  1:60394/1‑230 (MQ=255)                                                                                                                                                                                                     aatcaaatTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTATATTTTTCAGCTAATTGACGGATTAATTCACTACGATGc                                 <  1:301109/251‑1 (MQ=255)                                                                                                                                                                                                       tcaaatTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTATATTTTTCAGCTAATTGACGGATTAATTCACTACGATGCTc                               <  1:25594/251‑1 (MQ=255)                                                                                                                                                                                                          aatTGGAAATCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTATATTTTTCAGCTAATTGACGGATTAATTCACTACGATGCTCATc                            <  1:7711/251‑1 (MQ=255)                                                                                                                                                                                                                  aTCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTATATTTTTCAGCTAATTGACGGATTAATTCACTACGATGCTCATCGGGATAAc                    <  1:596813/251‑1 (MQ=255)                                                                                                                                                                                                                   tCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCaga                                                                                                                >  1:551461/1‑158 (MQ=255)                                                                                                                                                                                                                   tCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCt                                                                                 <  1:232292/189‑1 (MQ=255)                                                                                                                                                                                                                   tCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTATATTTTTCAGCTAATTGACGGATTAATTCACTACGATGCTCATCGGGATAACg                   >  1:182271/1‑251 (MQ=255)                                                                                                                                                                                                                   tCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTATATTTTTCAGCTAATTGACGGATTAATTCACTACGATGCTCATCGGGATAACg                   >  1:380173/1‑251 (MQ=255)                                                                                                                                                                                                                   tCTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTATATTTTTCAGCTAATTGACGGATTAATTCACTACGATGCTCATCGGGATAACg                   >  1:521547/1‑251 (MQ=255)                                                                                                                                                                                                                    cTTTAGTCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTATATTTTTCAGCTAATTGACGGATTAATTCACTACGATGCTCATCGGGATAACgg                  <  1:422756/251‑1 (MQ=255)                                                                                                                                                                                                                          tCAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTATATTTTTCAGCTAATTGACGGATTAATTCACTACGATGCTCATCGGGATAACGGGAAc              <  1:636220/249‑1 (MQ=255)                                                                                                                                                                                                                           cAGCGGCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGAt                                                                          <  1:217399/188‑1 (MQ=255)                                                                                                                                                                                                                                gCACCTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTATATTTTTCAGCTAATTGACGGATTAATTCACTACGATGCTCATCGGGATAACGGGAACCAATCCCa      >  1:777653/1‑251 (MQ=255)                                                                                                                                                                                                                                   ccTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTATATTTTTCAGCTAATTGACGGATTAATTCACTACGATGCTCATCGGGATAACGGGAAc              >  1:820594/1‑240 (MQ=255)                                                                                                                                                                                                                                    cTTAACAACCGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTATATTTTTCAGCTAATTGACGGATTAATTCACTACGATGCTCATCGGGATAACGGGAACCAATCCCAATGc  <  1:686385/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  CCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCCAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTTCTTGTAATTTTGCCAAATCAAATTGGAAATCTTTAGTCAGCGGCACCTTAACAACTGGCACATTCATGGCTTGCGCATATAATTCGGCATAATTAAACGTCGGATCCGGCACCACCAGCTGAACCGTCTGTTTCGCCTGTCTGGCTTTAAATACCTGTGACTGAATAATTCCACGAATATTTTCAGAGGATCCATTCCCCAAGGAAACGAAATTATCTTCCAGATTATATTTTTCAGCTAATTGACGGATTAATTCACTACGATGCTCATCGGGATAACGGGAACCAATCCCAATGC  >  1/1945215‑1945692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑