Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,945,178	A→G	A230A (GCT→GCC)	unknown ←	Periplasmic aromatic amino acid aminotransferase beta precursor (EC 2.6.1.57)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,945,178	0	A	G	100.0%	229.4 / NA	79	A230A (GCT→GCC)	unknown	Periplasmic aromatic amino acid aminotransferase beta precursor (EC 2.6.1.57)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/42);  total (37/42)

GTGCAAATTTTAAACCCAGTTCTCGCAATGCGTTTTGCACGATTTTACGGGAGGCTTCGTTGCTTTGTAAGCTCAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACAGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCCAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTT  >  1/1944931‑1945395                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                          gTGCAAATTTTAAACCCAGTTCTCGCAATGCGTTTTGCACGATTTTACGGGAGGCTTCGTTGCTTTGTAAGCTCAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCa                                                                                                                                                                                                                        >  1:660869/1‑251 (MQ=255)     gcttcatgctataatttttcGCAATGCGTTTTGCACGATTTTACGGGAGGCTTCGTTGCTTTGTAAGCTCAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACAt                                                                                                                                                                                                                    >  1:95044/19‑251 (MQ=255)                ccAGTTCTCGCAATGCGTTTTGCACGATTTTACGGGAGGCTTCGTTGCTTTGTAAGCTCAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAAccc                                                                                                                                                                                                         <  1:757093/251‑1 (MQ=255)                        cGCAATGCGTTTTGCACGATTTTACGGGAGGCTTCGTTGCTTTGTAAGCTCAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAAt                                                                                                                                                                                                 >  1:273063/1‑251 (MQ=255)                                  tttGCAAGATTTTACGGGAGGCTTCGTTGCTTTGTAAGCTCAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCGCCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCAAACATATCCCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACg                                                                                                                                                                                       <  1:389499/251‑1 (MQ=255)                                          aTTTTACGGGAGGCTTCGTTGCTTTGTAAGCTCAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATc                                                                                                                                                                                                >  1:866339/1‑234 (MQ=255)                                          aTTTTACGGGAGGCTTCGTTGCTTTGTAAGCTCAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCtt                                                                                                                                                                                           >  1:768592/1‑239 (MQ=255)                                                         tCGTTGCTTTGTAAGCTCAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATc                                                                                                                                                                   >  1:64941/1‑248 (MQ=255)                                                         tCGTTGCTTTGTAAGCTCAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTg                                                                                                                                                                <  1:811213/251‑1 (MQ=255)                                                            ttGCTTTGTAAGCTCAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTAc                                                                                                                                                             <  1:162939/251‑1 (MQ=255)                                                            ttGCTTTGTAAGCTCAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTAc                                                                                                                                                             <  1:794593/251‑1 (MQ=255)                                                            ttGCTTTGTAAGCTCAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGGCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTAc                                                                                                                                                             <  1:444550/251‑1 (MQ=255)                                                                 ttGTAAGCTCAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATAtt                                                                                                                                                        >  1:31574/1‑251 (MQ=255)                                                                  tGTAAGCTCAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTc                                                                                                                                                       <  1:371355/251‑1 (MQ=255)                                                                          cAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTc                                                                                                                                                       <  1:350115/243‑1 (MQ=255)                                                                             ttGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCt                                                                                                                                             <  1:718584/250‑1 (MQ=255)                                                                                ggAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCAt                                                                                                                                         >  1:39427/1‑251 (MQ=255)                                                                                  agaAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATc                                                                                                                                        <  1:585111/250‑1 (MQ=255)                                                                                    aaaGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATct                                                                                                                                       <  1:712195/249‑2 (MQ=255)                                                                                    aaaGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATct                                                                                                                                       <  1:730327/249‑2 (MQ=255)                                                                                         tttGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTaaaa                                                                                                                                <  1:855552/251‑1 (MQ=255)                                                                                            gTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGa                                                                                                                             >  1:501650/1‑251 (MQ=255)                                                                                                  cAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCt                                                                                                                                             >  1:514641/1‑229 (MQ=255)                                                                                                                   cAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATc                                                                                                                                        >  1:719430/1‑217 (MQ=255)                                                                                                                        gCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATAtt                                                                                                                                                        <  1:294415/196‑1 (MQ=255)                                                                                                                         cACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCTCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATc                                                                                                   <  1:446807/248‑1 (MQ=255)                                                                                                                            cGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTAc                                                                                                                                                             <  1:569180/187‑1 (MQ=255)                                                                                                                            cGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACgg                                                                                             <  1:555250/251‑1 (MQ=255)                                                                                                                                 aTGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATaa                                                                                        >  1:720254/1‑251 (MQ=255)                                                                                                                                 aTGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATaa                                                                                        >  1:684728/1‑251 (MQ=255)                                                                                                                                  tGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTAc                                                                                                                                                             <  1:781705/181‑1 (MQ=255)                                                                                                                                  tGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATaa                                                                                        >  1:880658/1‑250 (MQ=255)                                                                                                                                    ttGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAActc                                                                                     <  1:57905/251‑1 (MQ=255)                                                                                                                                      gATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCtt                                                                                   >  1:378729/1‑251 (MQ=255)                                                                                                                                       atatTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTAc                                                                                                                                                             <  1:398992/176‑1 (MQ=255)                                                                                                                                           tGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTg                                                                                <  1:807601/249‑1 (MQ=255)                                                                                                                                           tGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGc                                                                               >  1:30659/1‑250 (MQ=255)                                                                                                                                                   gACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTc                                                                                                                                                       <  1:882589/170‑1 (MQ=255)                                                                                                                                                    aCATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACg                                                                     <  1:346412/251‑1 (MQ=255)                                                                                                                                                     cATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACgg                                                                    <  1:343037/251‑1 (MQ=255)                                                                                                                                                       tGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGtt                                                                  >  1:189207/1‑251 (MQ=255)                                                                                                                                                         aaTTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGa                                                                >  1:149746/1‑251 (MQ=255)                                                                                                                                                           tttATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAg                                                              <  1:875850/251‑1 (MQ=255)                                                                                                                                                              aTCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATAtt                                                                                                                                                        >  1:773028/1‑158 (MQ=255)                                                                                                                                                                 cACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGat                                                        <  1:18938/251‑1 (MQ=255)                                                                                                                                                                     tcttcCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGat                                                        >  1:637609/1‑247 (MQ=255)                                                                                                                                                                     tcttcCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCCAGTAAAAAGa                                                                                                                             >  1:821198/1‑178 (MQ=255)                                                                                                                                                                     tcttcCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAG‑AAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGattatt                                                    >  1:619790/1‑250 (MQ=255)                                                                                                                                                                      cttcCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGatta                                                      >  1:366023/1‑248 (MQ=255)                                                                                                                                                                           accacATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGtt                                              <  1:474311/251‑1 (MQ=255)                                                                                                                                                                               caTCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAg                                                              >  1:459552/1‑231 (MQ=255)                                                                                                                                                                               caTCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTacac                                          <  1:556415/251‑1 (MQ=255)                                                                                                                                                                               caTCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTacac                                          <  1:266122/251‑1 (MQ=255)                                                                                                                                                                                     aaTTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAg                                    <  1:469370/251‑1 (MQ=255)                                                                                                                                                                                           ccAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAAt                               <  1:875741/250‑1 (MQ=255)                                                                                                                                                                                              gCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGAt                                                                                                                                                                    <  1:389937/114‑1 (MQ=255)                                                                                                                                                                                              gCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTa                                                                                                                                   >  1:136777/1‑147 (MQ=255)                                                                                                                                                                                              gCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGt                                                             >  1:578089/1‑217 (MQ=255)                                                                                                                                                                                              gCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACt                            >  1:33584/1‑250 (MQ=255)                                                                                                                                                                                              gCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACt                            >  1:241262/1‑250 (MQ=255)                                                                                                                                                                                               cATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGc                                                                               <  1:41464/198‑1 (MQ=255)                                                                                                                                                                                               cATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCa                          >  1:756492/1‑251 (MQ=255)                                                                                                                                                                                               cATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCa                          >  1:93463/1‑251 (MQ=255)                                                                                                                                                                                               cATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCa                          >  1:881853/1‑251 (MQ=255)                                                                                                                                                                                                   taCCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGAGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAAt                                                                                                    <  1:727697/173‑1 (MQ=255)                                                                                                                                                                                                          cgcgATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTaaa                >  1:368997/1‑250 (MQ=255)                                                                                                                                                                                                          cgcgATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAAc               <  1:61280/251‑1 (MQ=255)                                                                                                                                                                                                          cgcgATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTGCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAAc               <  1:395933/251‑1 (MQ=255)                                                                                                                                                                                                           gcgATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCa                                                                                                                                                                              <  1:136306/91‑1 (MQ=255)                                                                                                                                                                                                           gcgATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTaaa                >  1:511465/1‑249 (MQ=255)                                                                                                                                                                                                            cgATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTaaa                >  1:372637/1‑248 (MQ=255)                                                                                                                                                                                                              aTAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTAc                                                                                                                                                             <  1:448722/105‑1 (MQ=255)                                                                                                                                                                                                               tAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTc          <  1:615555/251‑1 (MQ=255)                                                                                                                                                                                                                       cATGGCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTAAATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGAAATAACTCTTTTGCCGGCGTAACGTTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGttt  <  1:22443/251‑1 (MQ=255)                                                                                                                                                                                                                          ggCATAAAATTTAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAAGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAAtt                                                                                                                      <  1:203893/132‑1 (MQ=255)                                                                                                                                                                                                                                     tAGAGAAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTc                           >  1:692600/1‑212 (MQ=255)                                                                                                                                                                                                                                       gagaAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAAt                                                                                                    >  1:358723/1‑137 (MQ=255)                                                                                                                                                                                                                                       gagaAAGTGCGGGTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGtt                                              <  1:7648/191‑1 (MQ=255)                                                                                                                                                                                                                                                  ggTGACGGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCTAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGat                                                        >  1:660652/1‑170 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                          GTGCAAATTTTAAACCCAGTTCTCGCAATGCGTTTTGCACGATTTTACGGGAGGCTTCGTTGCTTTGTAAGCTCAGTTGGGAGAAAGTTTTGTCTTTCAAGGAAGCAAGCGCGGCAACAGCACCGGCAATGTTGATATTGTCAATGGACATGAATTTATCCACTTCTTCCACCACATCGGAATTCGCCAGCATATACCCCACGCGATAACCTGCCATGGCATAAAATTTAGAGAAAGTGCGGGTGACAGCAACATTTTTAAAACCCTGTTTAATCAGCTTAACGCCACTTTCAAAAGCAGGATCCTGTACATATTCGGCGTAGGCTTCATCCAGTAAAAAGAAATAATTTTCCGGCGCTTTTTTAATCCAACGGAATAACTCTTTTGCCGGCGTAACGGTTGAAGTTGGATTATTCGGGTTACACAAATAGAAAATACTCACGCCACTAAACTGCTCGGCGGTTT  >  1/1944931‑1945395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑