Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,943,266	C→T	T452T (ACC→ACT)	unknown →	Uncharacterized protease YegQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,943,266	0	C	T	100.0%	134.2 / NA	48	T452T (ACC→ACT)	unknown	Uncharacterized protease YegQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/32);  total (16/32)

CGGCAAACGTAACGAACAAGGCATGGCGGAAGTGGCGGTGAAAAATAAATTCCTGCTTGGCGACGAAGTGGAAATAATGACACCGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACCAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACATTATTCAGCCCTTCCCCCTTGAAAGGGCTTTTTTTCGTCTTGTAGAAAGTAGAATTGAGTAAAAAATACCTTTAAGTATGCTCACAATTACCTGCTATTTGTATTGCTAA  >  1/1943029‑1943491                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                  cGGCAAACGTAACGAACAAGGCATGGCGGAAGTGGCGGTGAAAAATAAATTCCTGCTTGGCGACGAAGTGGAAATAATGACACCGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAAcc                                                                                                                                                                                                                      <  1:157870/251‑1 (MQ=255) cGGCAAACGTAACGAACAAGGCATGGCGGAAGTGGCGGTGAAAAATAAATTCCTGCTTGGCGACGAAGTGGAAATAATGACACCGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAAcc                                                                                                                                                                                                                      <  1:472558/251‑1 (MQ=255)       aCGTAACGAACAAGGCATGGCGGAAGTGGCGGTGAAAAATAAATTCCTGCTTGGCGACGAAGTGGAAATAATGACACCGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACg                                                                                                                                                                                                                <  1:816410/251‑1 (MQ=255)              gAACAAGGCATGGCGGAAGTGGCGGTGAAAAAAAAAATCCCGCTTGGCGACGAAGGGGGAAAAATGACACCGCAAAGCAATATTGTCTTCAAAAACAAAAAAAAGCTTTATCGCAAAAAAGTCGAAGTAGATGCCGCATTAGGCGACGGCAAATTTT‑TTTTTTTATAAGTGCCCCAAGAAATCCACCTTGG‑TTACGCGCCGTTTATTCGGAAACTGGTcaacactaaca                                                                                                                                                                                                                               >  1:106155/1‑229 (MQ=255)               aaCAAGGCATGGCGGAAGTGGCGGTGAAAAATAAATTCCTGCTTGGCGACGAAGGGGAAATAATTACACCGCAAGGCAATATCGTCTTCAAAATCAATAAAAAGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTTTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGGCAACACTAACACACGCAACCCCCACgaaaaaaaa                                                                                                                                                                                                        >  1:541155/1‑251 (MQ=255)                 cAAGGCATGGCGGAAGTGGCGGTGAAAAATAAATTCCTGCTTGGCGACGAAGTGGAAATAATGACACCGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGaaaagaaaag                                                                                                                                                                                                      <  1:496089/251‑1 (MQ=255)                                           aaataaatTCCTGCTTGGCGACGAAGTGGAAATAATGACACCGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGc                                                                                                                                                                            <  1:689484/251‑1 (MQ=255)                                                      tGCTTGGCGACGAAGTGGAAATAATGACACCGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAAttt                                                                                                                                                                 <  1:588770/251‑1 (MQ=255)                                                       gCTTGGCGACGAAGTGGAAATAATGACACCGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATc                                                                                                                                                                                   >  1:235924/1‑232 (MQ=255)                                                                cgaAGTGGAAATAATGACACCGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCtatat                                                                                                                                                       <  1:833675/251‑1 (MQ=255)                                                                    gTGGAAATAATGACACCGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATataata                                                                                                                                                   >  1:385547/1‑251 (MQ=255)                                                                     tGGAAATAATGACACCGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATataata                                                                                                                                                   <  1:830127/250‑1 (MQ=255)                                                                     tGGAAATAATGACACCGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATAc                                                                                                                                                  <  1:225460/251‑1 (MQ=255)                                                                       gAAATAATGACACCGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAatat                                                                                                                                                                                               >  1:897376/1‑204 (MQ=255)                                                                           taatGACACCGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATAc                                                                                                                                                  >  1:607677/1‑245 (MQ=255)                                                                                 cacCGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAatat                                                                                                                                                                                               <  1:382191/194‑1 (MQ=255)                                                                                   ccGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGa                                                                                                                                    >  1:579604/1‑251 (MQ=255)                                                                                      cAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGaaa                                                                                                                                  <  1:738456/250‑1 (MQ=255)                                                                                        aGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACga                                                                                                                                                                                                               >  1:898168/1‑171 (MQ=255)                                                                                        aGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCtatat                                                                                                                                                       <  1:255815/227‑1 (MQ=255)                                                                                        aGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGaaa                                                                                                                                  <  1:677760/248‑1 (MQ=255)                                                                                          gCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATAc                                                                                                                                                  <  1:504898/230‑1 (MQ=255)                                                                                          gCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGaaa                                                                                                                                  <  1:276501/246‑1 (MQ=255)                                                                                             atatCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAAc                                                                                                                          <  1:632086/251‑1 (MQ=255)                                                                                               atCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATAc                                                                                                                                          <  1:53567/233‑1 (MQ=255)                                                                                                           aTCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACattat                                                                                                            <  1:125940/251‑1 (MQ=255)                                                                                                             cAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATGACTCAAAATACATTATTc                                                                                                          <  1:307139/251‑1 (MQ=255)                                                                                                             cAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACATTATTc                                                                                                          <  1:200296/251‑1 (MQ=255)                                                                                                             cAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACATTATTc                                                                                                          <  1:314523/251‑1 (MQ=255)                                                                                                                    aTGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCtatat                                                                                                                                                       <  1:663252/199‑1 (MQ=255)                                                                                                                           aTCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACATTATTCAGCCCTTCCCCCtt                                                                                            >  1:1689/1‑251 (MQ=255)                                                                                                                               cAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACATTATTCAGCCCTTCCCCCTTGaaa                                                                                        <  1:854864/251‑1 (MQ=255)                                                                                                                                         gAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAatat                                                                                                                                                                                               <  1:434851/138‑1 (MQ=255)                                                                                                                                          aaGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTcaacactaacac                                                                                                                                                                                                                              >  1:314747/1‑106 (MQ=255)                                                                                                                                          aaGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACATTATTCAGCCCTTCCCCCTTGAAAGGGCtttttt                                                                              <  1:35951/250‑1 (MQ=255)                                                                                                                                            gTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTcaacactaacac                                                                                                                                                                                                                              >  1:694660/1‑104 (MQ=255)                                                                                                                                                   ccGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTg                                                                                                                                                                <  1:723399/159‑1 (MQ=255)                                                                                                                                                                               tcttAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAAcc                                                                                                                                                                                                                      >  1:821185/1‑78 (MQ=255)                                                                                                                                                                               tcttAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAAt                                                                                                                                                                                    >  1:557713/1‑112 (MQ=255)                                                                                                                                                                               tcttAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATAc                                                                                                                                                  <  1:860021/146‑1 (MQ=255)                                                                                                                                                                               tcttAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATAc                                                                                                                                                  >  1:352405/1‑146 (MQ=255)                                                                                                                                                                               tcttAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACATTATTCAGCCCTTCCCCCTTGAAAGGGCTTTTTTTCGTCTTGTAGAAAGTAGAATTGAGTAAAAAATAc                                           >  1:534096/1‑249 (MQ=255)                                                                                                                                                                                     aCGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACATTATTCAGCCCTTCCCCCTTGAAAGGGCTTTTTTTCGTCTTGTAGAAAGTAGAATTGAGTAAAAAATACCTTTAAGt                                   <  1:98289/251‑1 (MQ=255)                                                                                                                                                                                        tGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACATTATTc                                                                                                          >  1:222072/1‑177 (MQ=255)                                                                                                                                                                                                 aCAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACATTATTCAGCCCTTCCCCCTTGAAAGGGCTTTTTTTCGTCTTGTAGAAAGTAGAATTGAGTAAAAAATACCTTTAAGTATGCTCACaa                         <  1:778013/249‑1 (MQ=255)                                                                                                                                                                                                      cAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACATTATTCAGCCCTTCCCCCTTGAAAGGGCTTTTTTTCGTCTTGTAGAAAGTAGAATTGAGTAAAAAATACCTTTAAGTATGCTCACAATTACCt                   <  1:620608/251‑1 (MQ=255)                                                                                                                                                                                                            ggATTACGCGCTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAAtt                                                                                                                                                                  <  1:379227/102‑1 (MQ=255)                                                                                                                                                                                                                      cTGTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACATTATTCAGCCCTTCCCCCTTGAAAGGGCttttttt                                                                             >  1:686122/1‑177 (MQ=255)                                                                                                                                                                                                                        gTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACATTATTCAGCCCTTCCCCCTTGAAAGGGCTTTTTTTCGTCTTGTAGAAAGTAGAATTGAGTAAAAAATACCTTTAAg                                    <  1:87774/216‑1 (MQ=255)                                                                                                                                                                                                                        gTTAATGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACATTATTCAGCCCTTCCCCCTTGAAAGGGCTTTTTTTCGTCTTGTAGAAAGTAGAATTGAGTAAAAAATACCTTTAAGTATGCTCACAATTACCTGCTATTTGTATTGCTaa  >  1:400553/1‑250 (MQ=255)                                                                                                                                                                                                                             tGCGAAACCTGGTCAACACTAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACATTATTCAGCCCTTCCCCCTTGAAAGGGCTTTTTTTCGTCTTGTAGAAAGTAGAATTGAGTAAAAAATACCTTTAAGTATGCTCACAAt                        >  1:801719/1‑223 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                  CGGCAAACGTAACGAACAAGGCATGGCGGAAGTGGCGGTGAAAAATAAATTCCTGCTTGGCGACGAAGTGGAAATAATGACACCGCAAGGCAATATCGTCTTCAAAATCAATAAAATGCTTAATCGCAAAAACGTCGAAGTAGATGCCGCATTAGGCGACGG‑ACATTTTGTCTTCTTAGACGTGCCGCAAGACAT‑CAACTTGGATTACGCGCTGTTAATGCGAAACCTGGTCAACACCAACACACGCAACCCTCACGAAAAGAAAAGTTAATATTTTGTTAAAATCATGTTGCAATATTAATTTGGGTCTATATAATACGCACATACCCTAGAAAAATATAACTCAAAATACATTATTCAGCCCTTCCCCCTTGAAAGGGCTTTTTTTCGTCTTGTAGAAAGTAGAATTGAGTAAAAAATACCTTTAAGTATGCTCACAATTACCTGCTATTTGTATTGCTAA  >  1/1943029‑1943491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑