Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 257,324 T→C L412S (TTA→TCA)  unknown → Short‑chain fatty acids transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*1257,3240TC100.0% 332.2 / NA 115L412S (TTA→TCA) unknownShort‑chain fatty acids transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/69);  total (46/69)

Only 100 of 115 total aligned reads displayed.

GGGCTCGGCGGACTCATCACCGAATTCTTTATTAACATCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTTACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGCCGATCGGCAGCTTTAACGGCAGTCTTGCCGCCGTCAGTGCGGTTGATCTGGGCGCTACCGTGATCAAAGAAGTGATCA  >  1/257080‑257565
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
gggCTCGGCGGACTCATCACCGAATTCTTTATTAACATCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCgg                                                                                                                                                                                                                                               >  1:131682/1‑250 (MQ=255)
       gcggACTCATCACCGAATTCTTTATTAACATCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTggg                                                                                                                                                                                                                                        <  1:723377/250‑1 (MQ=255)
              catcaCCGAATTCTTTATTAACATCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGACGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑g                                                                                                                                                                                                                               >  1:464732/1‑251 (MQ=255)
                       aTTCTTTATTAACATCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTgggg                                                                                                                                                                                                                      >  1:477476/1‑251 (MQ=255)
                       aTTCTTTATTAACATCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTgggg                                                                                                                                                                                                                      <  1:88040/251‑1 (MQ=255)
                         tCTTTATTAACATCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTa                                                                                                                                                                                                                    <  1:793955/251‑1 (MQ=255)
                          cTTTATTAACATCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTgggg                                                                                                                                                                                                                      <  1:33785/248‑1 (MQ=255)
                          cTTTATTAACATCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTa                                                                                                                                                                                                                    >  1:671773/1‑250 (MQ=255)
                          cTTTATTAACATCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTa                                                                                                                                                                                                                    >  1:14602/1‑250 (MQ=255)
                           tttATTAACATCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACg                                                                                                                                                                                                                  >  1:172362/1‑251 (MQ=255)
                             tattaACATCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACggg                                                                                                                                                                                                                <  1:654074/251‑1 (MQ=255)
                             tattaACATCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACggg                                                                                                                                                                                                                <  1:70077/251‑1 (MQ=255)
                             tattaACATCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACggg                                                                                                                                                                                                                <  1:704284/251‑1 (MQ=255)
                               ttgaCATCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGat                                                                                                                                                                                                              <  1:863039/248‑1 (MQ=255)
                                    aTCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGAtattat                                                                                                                                                                                                         <  1:875777/251‑1 (MQ=255)
                                    aTCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGAtattat                                                                                                                                                                                                         <  1:106558/251‑1 (MQ=255)
                                    aTCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGAtattat                                                                                                                                                                                                         <  1:402517/251‑1 (MQ=255)
                                    aTCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGAtattat                                                                                                                                                                                                         <  1:591840/251‑1 (MQ=255)
                                    aTCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGAtattat                                                                                                                                                                                                         <  1:868070/251‑1 (MQ=255)
                                      cGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCATTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGACATCGCA‑GGTCTTGGGGTACGGGATATTATgg                                                                                                                                                                                                       <  1:446850/251‑1 (MQ=255)
                                      cGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACggg                                                                                                                                                                                                                <  1:786938/242‑1 (MQ=255)
                                      cGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATgg                                                                                                                                                                                                       <  1:873205/251‑1 (MQ=255)
                                      cGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATgg                                                                                                                                                                                                       <  1:898736/251‑1 (MQ=255)
                                         cAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCCACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGAt                                                                                                                                                                                                    <  1:590502/251‑1 (MQ=255)
                                          aaTAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTgggg                                                                                                                                                                                                                      >  1:825553/1‑232 (MQ=255)
                                            tAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGAtt                                                                                                                                                                                                   >  1:360587/1‑249 (MQ=255)
                                                 acacCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTa                                                                                                                                                                                              <  1:473996/249‑1 (MQ=255)
                                                   cccTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGAc                                                                                                                                                                                          <  1:391743/250‑1 (MQ=255)
                                                   cccTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGAc                                                                                                                                                                                          <  1:233963/250‑1 (MQ=255)
                                                     cTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACggg                                                                                                                                                                                                                <  1:376481/227‑1 (MQ=255)
                                                       tCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTc                                                                                                                                                                                                                            <  1:499872/213‑1 (MQ=255)
                                                       tCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGatat                                                                                                                                                                                                            <  1:592855/229‑1 (MQ=255)
                                                       tCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATg                                                                                                                                                                                            <  1:265804/245‑1 (MQ=255)
                                                       tCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCa                                                                                                                                                                                      >  1:163918/1‑251 (MQ=255)
                                                       tCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAA‑TGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGcgc                                                                                                                                                                                     <  1:529082/251‑3 (MQ=255)
                                                            gTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCCACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTAt                                                                                                                                                                                 >  1:430986/1‑251 (MQ=255)
                                                            gTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTgggg                                                                                                                                                                                                                      <  1:232093/214‑1 (MQ=255)
                                                               cTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCt                                                                                                                                                                               <  1:394060/250‑1 (MQ=255)
                                                                tGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTc                                                                                                                                                                             <  1:382352/251‑1 (MQ=255)
                                                                  aCCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTcac                                                                                                                                                                           <  1:335674/251‑1 (MQ=255)
                                                                     ttcttcAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTcacca                                                                                                                                                                         >  1:791504/1‑250 (MQ=255)
                                                                      tcttcAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGAc                                                                                                                                                                                          <  1:358356/232‑1 (MQ=255)
                                                                       cttcAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCAccc                                                                                                                                                                      <  1:300126/251‑1 (MQ=255)
                                                                       cttcAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGACGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCaccacc                                                                                                                                                                       >  1:429435/1‑250 (MQ=255)
                                                                            gCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTgggg                                                                                                                                                                                                                      <  1:539105/198‑1 (MQ=255)
                                                                             cTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATc                                                                                                                                                                 <  1:332934/250‑1 (MQ=255)
                                                                               ccGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATc                                                                                                                                                                                >  1:165501/1‑233 (MQ=255)
                                                                                 gCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTggg                                                                                                                                                                                                                       <  1:618751/192‑1 (MQ=255)
                                                                                 gCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGat                                                                                                                                                                                                              >  1:288588/1‑201 (MQ=255)
                                                                                 gCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGAtattat                                                                                                                                                                                                         <  1:109995/206‑1 (MQ=255)
                                                                                    ttAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGt                                                                                                                                                                                                                                            >  1:654004/1‑169 (MQ=255)
                                                                                      aaTCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGTAATTGCCTACGGCGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCAccc                                                                                                                                                                      >  1:85335/1‑236 (MQ=255)
                                                                                      aaTCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTcac                                                                                                                                                                           >  1:335318/1‑231 (MQ=255)
                                                                                      aaTCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCg                                                                                                                                                       >  1:841090/1‑251 (MQ=255)
                                                                                              tCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTatat                                                                                                                                               <  1:59177/251‑1 (MQ=255)
                                                                                               cGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAATAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATAtt                                                                                                                                              <  1:320989/251‑1 (MQ=255)
                                                                                                        gtCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGAtatt                                                                                                                                                                                                           <  1:281107/181‑1 (MQ=255)
                                                                                                        gtCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGAc                                                                                                                                                                                          >  1:640283/1‑198 (MQ=255)
                                                                                                                 cggGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAgg                                                                                                                             <  1:314049/250‑1 (MQ=255)
                                                                                                                 cggGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAgg                                                                                                                             <  1:422371/250‑1 (MQ=255)
                                                                                                                  gggCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATc                                                                                                                                                                                >  1:782024/1‑198 (MQ=255)
                                                                                                                    gCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGAt                                                                                                                                                                                                    >  1:645853/1‑176 (MQ=255)
                                                                                                                       cTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACaaa                                                                                                                      <  1:93899/251‑1 (MQ=255)
                                                                                                                               ttCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTgggg                                                                                                                                                                                                                      <  1:2788/147‑1 (MQ=255)
                                                                                                                               ttCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTAc                                                                                                                                                                                                                   <  1:434364/150‑1 (MQ=255)
                                                                                                                               ttCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGaaa                                                                                                              >  1:478627/1‑251 (MQ=255)
                                                                                                                                     ggCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACggg                                                                                                                                                                                                                <  1:827676/147‑1 (MQ=255)
                                                                                                                                                         gcACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTg                                                                                                                                                                                                                                          >  1:265470/1‑102 (MQ=255)
                                                                                                                                                         gcACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAat                                                                                                                     >  1:217700/1‑218 (MQ=255)
                                                                                                                                                          cacaGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGaa                                                                                   <  1:666823/251‑1 (MQ=255)
                                                                                                                                                               gCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGc                                                                                <  1:415171/249‑1 (MQ=255)
                                                                                                                                                                 aTTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTatat                                                                                                                                               >  1:195950/1‑184 (MQ=255)
                                                                                                                                                                 aTTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGCCGAt                                                                            <  1:157153/251‑1 (MQ=255)
                                                                                                                                                                    gggCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCAccc                                                                                                                                                                      >  1:375821/1‑158 (MQ=255)
                                                                                                                                                                    gggCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCAccc                                                                                                                                                                      >  1:52148/1‑158 (MQ=255)
                                                                                                                                                                    gggCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGCCGATCgg                                                                         >  1:320365/1‑251 (MQ=255)
                                                                                                                                                                           gATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAata                                                                                                                    <  1:306598/201‑1 (MQ=255)
                                                                                                                                                                           gATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGCCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTatat                                                                                                                                               <  1:75799/174‑1 (MQ=255)
                                                                                                                                                                              cTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTcac                                                                                                                                                                           <  1:562487/143‑1 (MQ=255)
                                                                                                                                                                                 ggCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGCCGATCGGCAGCTTTAACgg                                                             <  1:311973/250‑1 (MQ=255)
                                                                                                                                                                                             aTGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATc                                                                                                                                                                                <  1:275048/123‑1 (MQ=255)
                                                                                                                                                                                               ggCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGAc                                                                                                                                                                                          >  1:22513/1‑111 (MQ=255)
                                                                                                                                                                                                     tGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGaa                                                                                   <  1:542649/208‑1 (MQ=255)
                                                                                                                                                                                                     tGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGc                                                                                >  1:832098/1‑211 (MQ=255)
                                                                                                                                                                                                      gCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGCCGATCGGCAGCTTTAACGGCAGTCTTGCCGCCGTCAGTGCg                                       >  1:701024/1‑251 (MQ=255)
                                                                                                                                                                                                       ccTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCAGGGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTatat                                                                                                                                               <  1:698295/147‑1 (MQ=255)
                                                                                                                                                                                                             gTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTa                                                                                                                                                                                  <  1:732495/105‑1 (MQ=255)
                                                                                                                                                                                                                     cggATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGaaaa                                                                                                             <  1:338069/165‑1 (MQ=255)
                                                                                                                                                                                                                      ggATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACgg                                                                                                                                                                                                                 <  1:439202/65‑1 (MQ=255)
                                                                                                                                                                                                                       gATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGCCGATCGGCAGCTTTAACGGCAGTCTTgccg                                                  >  1:591748/1‑223 (MQ=255)
                                                                                                                                                                                                                       gATGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGCCGATCGGCAGCTTTAACGGCAGTCTTGCCGCCGTCAGTGCGGTTGATCTGGGCGCTa                       >  1:214971/1‑250 (MQ=255)
                                                                                                                                                                                                                         tGAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGCCGATCGGCAGCTTTAACGGCAGTCTTGCCGCCGTCAGTGCGGTTGATCTGGGCGCTACCg                    <  1:675720/251‑1 (MQ=255)
                                                                                                                                                                                                                          gAACATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGCCGATCGGCAGCTTTAACgg                                                             >  1:479982/1‑209 (MQ=255)
                                                                                                                                                                                                                            aCATGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGCCGATCGGCAGCTTTAACGGCAGTCTTGCCGCCGTCAGTGCGGTTGATCTGGGCGCTACCGTGa                 >  1:795764/1‑251 (MQ=255)
                                                                                                                                                                                                                               tGGCGCAACCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGCCGATCGGCAGCTTTAACGGCAGTCTTGCCGCCGTc                                             >  1:803776/1‑220 (MQ=255)
                                                                                                                                                                                                                                      aCCGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGAc                                                                                                                                                                                          <  1:786400/72‑1 (MQ=255)
                                                                                                                                                                                                                                       ccGTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGCCGATCGGCAGCTTTAACGGCAGTCTTGCCGCCGTCAGTGCGGTTGATCTGGGCGCTACCGTGATc               <  1:375925/242‑1 (MQ=255)
                                                                                                                                                                                                                                         gTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGCCGATCGGCAGCTTTAACGGCAGTCTTGCCGCCGTCAGTGCGGTTGATCTGGGCGCTACCGTGATCAAAGAAGTGAt    >  1:226293/1‑251 (MQ=255)
                                                                                                                                                                                                                                         gTTCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGCCGATCGGCAGCTTTAACGGCAGTCTTGCCGCCGTCAGTGCGGTTGATCTGGGCGCTACCGTGATCAAAGAAGTGAt    >  1:551712/1‑251 (MQ=255)
                                                                                                                                                                                                                                           tCTGGGCGTCACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCATCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGCCGATCGGCAGCTTTAACGGCAGTCTTGCCGCCGTCAGTGCGGTTGATCTGGGCGCTACCGTGATCAAAGAAGTGATCa  <  1:453003/251‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
GGGCTCGGCGGACTCATCACCGAATTCTTTATTAACATCGCCAATAAAGACACCTTCCCGGTGCTGACCTTCTTCAGCTCCGCATTAATCAACTTCGCCGTGCCGTCCGGCGGCGGGCACTGGGTGATTCAAGGGCCGTTCGTCATTCCGGCGGCACAGGCATTGGGCGCAGATCTGGGCAAATCCACTATGGCAATTGCCTACGGTGAACAATGGATGAACATGGCGCAACCGTTCTGGGCGTTACCGGCGTTGGGCATCGCA‑GGTCTTGGGGTACGGGATATTATGGGATTCTGTATGACCGCACTTATCTTCACCACCCCGATCTTTTTCATCGGATTATATTTCTTTTAAATTAAGAGGATACAAATATGGAAAATATCGTTATCGTCAGCGCGGTCAGAACGCCGATCGGCAGCTTTAACGGCAGTCTTGCCGCCGTCAGTGCGGTTGATCTGGGCGCTACCGTGATCAAAGAAGTGATCA  >  1/257080‑257565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑