Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,923,792	C→A	I6I (ATC→ATA)	unknown →	Phosphate transport regulator (distant homolog of PhoU)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,923,792	0	C	A	100.0%	194.4 / NA	68	I6I (ATC→ATA)	unknown	Phosphate transport regulator (distant homolog of PhoU)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/32);  total (36/32)

GTGCTACATCGACAAAATCCACCGCTAACTTTAATTCAATTTCATCCTGCATTATAAAATCCCTTAAAGATCATGAGGTTTTATCTTCTTACCTGATTTATAAGGGATTTATTATCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATCCTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCATGCCTATCGATCGTACGGATTTGTTGGAATTA  >  1/1923559‑1924038                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       gtgCTACATCGACAAAATCCACCGCTAACTTTAATTCAATTTCATCCTGCATTATAAAATCCCTTAAAGATCATGAGGTTTTATCTTCTTACCTGATTTATAAGGGATTTATTATCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCg                                                                                                                                                                                                                                                    <  1:250649/238‑1 (MQ=255)              aaaaTCCACCGCTAACTTTAATTCAATTTCATCCTGCATTATAAAATCCCTTAAAGATCATGAGGTTTTATCTTCTTACCTGATTTATAAGGGATTTATTATCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAg                                                                                                                                                                                                                          <  1:279406/251‑1 (MQ=255)                aaTCCACCGCTAACTTTAATTCAATTTCATCCTGCATTATAAAATCCCTTAAAGATCATGAGGTTTTATCTTCTTACCTGATTTATAAGGGATTTATTATCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGcc                                                                                                                                                                                                                        <  1:555112/251‑1 (MQ=255)                           aaCTTTAATTCAATTTCATCCTGCATTATAAAATCCCTTAAAGATCATGAGGTTTTATCTTCTTACCTGATTTATAAGGGATTTATTATCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAac                                                                                                                                                                                                             <  1:805144/251‑1 (MQ=255)                                       aTTTCATCCTGCATTATAAAATCCCTTAAAGATCATGAGGTTTTATCTTCTTACCTGATTTATAAGGGATTTATTATCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGatt                                                                                                                                                                                                                                                <  1:560990/204‑1 (MQ=255)                                                  cATTATAAAATCCCTTAAAGATCATGAGGTTTTATCTTCTTACCTGATTTATAAGGGATTTATTATCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAAtgt                                                                                                                                                                                      <  1:210972/251‑1 (MQ=255)                                                    ttATAAAATCCCTTAAAGATCATGAGGTTTTATCTTCTTACCTGATTTATAAGGGATTTATTATCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAAtgttg                                                                                                                                                                                    >  1:419762/1‑251 (MQ=255)                                                      ataAAATCCCTTAAAGATCATGAGGTTTTATCTTCTTACCTGATTTATAAGGGATTTATTATCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTg                                                                                                                                                                                                                                           <  1:293109/194‑1 (MQ=255)                                                      ataAAATCCCTTAAAGATCATGAGGTTTTATCTTCTTACCTGATTTATAAGGGATTTATTATCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAg                                                                                                                                                                                                 <  1:53322/236‑1 (MQ=255)                                                           aTCCCTTAAAGATCATGAGGTTTTATCTTCTTACCTGATTTATAAGGGATTTATTATCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAac                                                                                                                                                                                                             <  1:412991/219‑1 (MQ=255)                                                                  aaaGATCATGAGGTTTTATCTTCTTACCTGATTTATAAGGGATTTATTATCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATcc                                                                                                                                                                      <  1:524897/251‑1 (MQ=255)                                                                       tCATGAGGTTTTATCTTCTTACCTGATTTATAAGGGATTTATTATCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCtttt                                                                                                                                                                 >  1:734813/1‑251 (MQ=255)                                                                             ggTTTTATCTTCTTACCTGATTTATAAGGGATTTATTATCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAAtgt                                                                                                                                                                                      >  1:226886/1‑224 (MQ=255)                                                                                                             ttattaTCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTccc                                                                                                                                                                                                                                  >  1:229157/1‑148 (MQ=255)                                                                                                             ttattaTCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAg                                                                                                                                                                                                                          <  1:514410/156‑1 (MQ=255)                                                                                                                    ccAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGAc                                                                                                                                         <  1:673519/230‑1 (MQ=255)                                                                                                                       ataaataaGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAat                                                                                                                                                                               >  1:519207/1‑189 (MQ=255)                                                                                                                         aaataaGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAg                                                                                                                                                                                                 <  1:685889/169‑1 (MQ=255)                                                                                                                                                              cACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAAtgt                                                                                                                                                                                      >  1:553105/1‑143 (MQ=255)                                                                                                                                                                   aCATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCttt                                                                                                                                                                                                                              >  1:287903/1‑98 (MQ=255)                                                                                                                                                                   aCATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAat                                                                                                                                                                               >  1:100928/1‑145 (MQ=255)                                                                                                                                                                   aCATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAAttatta                                                                                                                                                                          <  1:238541/150‑1 (MQ=255)                                                                                                                                                                   aCATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAAttatta                                                                                                                                                                          <  1:731254/150‑1 (MQ=255)                                                                                                                                                                   aCATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACAttt                                                                                                                                                  >  1:452548/1‑174 (MQ=255)                                                                                                                                                                   aCATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATAc                                                                                                                                             <  1:195927/179‑1 (MQ=255)                                                                                                                                                                   aCATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGata                                                                                                          <  1:277924/214‑1 (MQ=255)                                                                                                                                                                   aCATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTc                                                                                                      >  1:456768/1‑218 (MQ=255)                                                                                                                                                                   aCATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACg                                                                     >  1:329067/1‑251 (MQ=255)                                                                                                                                                                      tAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATAc                                                                                                                                             <  1:378721/176‑1 (MQ=255)                                                                                                                                                                       aaTGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAg                                                                                                                               <  1:507473/189‑1 (MQ=255)                                                                                                                                                                         tGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCt                                                                                   >  1:213083/1‑231 (MQ=255)                                                                                                                                                                         tGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAAc                                                                      >  1:777767/1‑244 (MQ=255)                                                                                                                                                                                    cgTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTaaaa                                                     <  1:348290/250‑1 (MQ=255)                                                                                                                                                                                         attattAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAg                                                                                                                                                                                                                          <  1:139230/80‑1 (MQ=255)                                                                                                                                                                                          ttattaACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAattat                                                                                                                                                                            <  1:216108/125‑1 (MQ=255)                                                                                                                                                                                          ttattaACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACAt                                                                                                                                                    <  1:311832/149‑1 (MQ=255)                                                                                                                                                                                          ttattaACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATAc                                                                                                                                             <  1:93871/156‑1 (MQ=255)                                                                                                                                                                                          ttattaACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGttt                                                                                                               >  1:137436/1‑186 (MQ=255)                                                                                                                                                                                          ttattaACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACcg                                               >  1:507038/1‑250 (MQ=255)                                                                                                                                                                                          ttattaACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTGACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGGTAAAACGTGAGTTTCGTTTAAAATTACcg                                               >  1:176564/1‑250 (MQ=255)                                                                                                                                                                                                    tccttAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTAtt                                    >  1:244142/1‑251 (MQ=255)                                                                                                                                                                                                    tccttAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTAtt                                    >  1:513940/1‑251 (MQ=255)                                                                                                                                                                                                    tccttAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTAtt                                    >  1:865429/1‑251 (MQ=255)                                                                                                                                                                                                    tccttAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTAtt                                    >  1:275042/1‑251 (MQ=255)                                                                                                                                                                                                     ccttAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTg                                                                                                                                                             >  1:831096/1‑129 (MQ=255)                                                                                                                                                                                                     ccttAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAAt                                                                                                                                               <  1:205034/143‑1 (MQ=255)                                                                                                                                                                                                     ccttAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGAcc                                                                                                                                        >  1:95952/1‑149 (MQ=255)                                                                                                                                                                                                     ccttAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTAtt                                    >  1:551898/1‑250 (MQ=255)                                                                                                                                                                                                     ccttAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTAtt                                    >  1:661512/1‑250 (MQ=255)                                                                                                                                                                                                      cttAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCa                                  <  1:571222/251‑1 (MQ=255)                                                                                                                                                                                                      cttAAAGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCa                                  <  1:381216/251‑1 (MQ=255)                                                                                                                                                                                                          aaGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCATGcc                              <  1:60575/251‑1 (MQ=255)                                                                                                                                                                                                          aaGGAGTCACTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCATGcc                              <  1:295880/251‑1 (MQ=255)                                                                                                                                                                                                                  aCTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCa                                  >  1:768888/1‑239 (MQ=255)                                                                                                                                                                                                                   cTTTTTATGGCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCATGCCTatcgat                       >  1:657085/1‑249 (MQ=255)                                                                                                                                                                                                                            gCAATGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCATGcc                              >  1:163650/1‑233 (MQ=255)                                                                                                                                                                                                                               aTGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTg                                                                                                    >  1:676676/1‑160 (MQ=255)                                                                                                                                                                                                                               aTGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCATGCCTatcgat                       <  1:121152/237‑1 (MQ=255)                                                                                                                                                                                                                                tGAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCATGCCTATCGATCGTACGGATttgttg        <  1:165848/251‑1 (MQ=255)                                                                                                                                                                                                                                 gAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAg                                                                                                                               >  1:94344/1‑131 (MQ=255)                                                                                                                                                                                                                                 gAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTc                                   <  1:424151/223‑1 (MQ=255)                                                                                                                                                                                                                                 gAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCATGCCTatcgat                       >  1:117631/1‑235 (MQ=255)                                                                                                                                                                                                                                 gAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCATGCCTATCGATCGTACGGATTTGTTgg       >  1:364308/1‑251 (MQ=255)                                                                                                                                                                                                                                 gAATAATATACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCATGCCTATCGATCGTACGGATTTGTTgg       >  1:138112/1‑251 (MQ=255)                                                                                                                                                                                                                                   ataataTACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCa                                  >  1:628350/1‑222 (MQ=255)                                                                                                                                                                                                                                    taataTACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGACTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCATGCCTATCGATCGTACGGATTTGTTGGAAt    >  1:423082/1‑251 (MQ=255)                                                                                                                                                                                                                                     aataTACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGc                                                                                                                                                         >  1:332331/1‑101 (MQ=255)                                                                                                                                                                                                                                      atatACTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCATGCCTATCGATCGTACGGATTTGTTGGAATTa  >  1:403865/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       GTGCTACATCGACAAAATCCACCGCTAACTTTAATTCAATTTCATCCTGCATTATAAAATCCCTTAAAGATCATGAGGTTTTATCTTCTTACCTGATTTATAAGGGATTTATTATCCAATAAATAAGGGGATTTTTCGATTAAAATCGTGAATTTTTCACCAACATAATGTAATATGCGCGTGAATTATTAACCTTCCTTAAAGGAGTCACTTTTTATGGCAATGAATAATATCCTCGGATTATTTGCCCACTCCCCTTTAAAGCCTCTGCAAAAACACTCTAATAAAGTTACCGAATGTTGTGAATTATTAATCCCTTTTTTTGAAGCAACATTTAATACCGACTGGGTAAAAGCGGAGCAAATTCGTTTAGATATTTCTGCACACGAACGTCAGGCTGACGCGTTAAAACGTGAGATTCGTTTAAAATTACCGCGCGGTTTATTCATGCCTATCGATCGTACGGATTTGTTGGAATTA  >  1/1923559‑1924038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑