Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,910,277	A→G	M337V (ATG→GTG)	unknown →	Spermidine/putrescine import ABC transporter substrate‑binding protein PotD (TC 3.A.1.11.1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,910,277	0	A	G	100.0%	226.9 / NA	78	M337V (ATG→GTG)	unknown	Spermidine/putrescine import ABC transporter substrate‑binding protein PotD (TC 3.A.1.11.1)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/46);  total (32/46)

GGCACCTTTAGACATGGTGTTCCCGAAAGAAGGTCCGGTCCTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACATGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCTAAGATAAGAAAAATAATACGCAAAAAGGAGATTTTATGTCATCCCACATTTCAGACAAGATTCAAGA  >  1/1910039‑1910519                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   ggCACCTTTAGACATGGTGTTCCCGAAAGAAGGTCCGGTCCTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACaaaaaa                                                                                                                                                                                                                                        >  1:644070/1‑251 (MQ=255)     ccTTTAGACATGGTGTTCCCGAAAGAAGGTCCGGTCCTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACaaaaa                                                                                                                                                                                                                                         <  1:94379/246‑1 (MQ=255)     ccTTTAGACATGGTGTTCCCGAAAGAAGGTCCGGTCCTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTa                                                                                                                                                                                                                                    >  1:92962/1‑251 (MQ=255)     ccTTTAGACATGGTGTTCCCGAAAGAAGGTCCGGTCCTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTa                                                                                                                                                                                                                                    >  1:735046/1‑251 (MQ=255)     ccTTTAGACATGGTGTTCCCGAAAGAAGGTCCGGTCCTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGGGCTACAAAAAAGCTa                                                                                                                                                                                                                                    >  1:606004/1‑251 (MQ=255)         tAGACATGGTGTTCCCGAAAGAAGGTCCGGTCCTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTgg                                                                                                                                                                                                                                >  1:105001/1‑251 (MQ=255)                 gtgtTCCCGAAAGAAGGTCCGGTCCTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAgatga                                                                                                                                                                                                                        >  1:6340/1‑251 (MQ=255)                 gtgtTCCCGAAAGAAGGTCCGGTCCTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAgatg                                                                                                                                                                                                                         <  1:144431/250‑1 (MQ=255)                 gtgtTCCCGAAAGAAGGTCCGGTCCTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAgatg                                                                                                                                                                                                                         <  1:544014/250‑1 (MQ=255)                    ttCCCGAAAGAAGGTCCGGTCCTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGatgatg                                                                                                                                                                                                                      <  1:886047/250‑1 (MQ=255)                    ttCCCGAAAGAAGGTCCGGTCCTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGatgatg                                                                                                                                                                                                                      <  1:213210/250‑1 (MQ=255)                     tCCCGAAAGAAGGTCCGGTCCTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGAtgtg                                                                                                                                                                                                                    >  1:303749/1‑251 (MQ=255)                        cGAAAGAAGGTCCGGTCCTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGc                                                                                                                                                                                                                 <  1:332434/251‑1 (MQ=255)                                       tccTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAAtac                                                                                                                                                                                                  <  1:528282/251‑1 (MQ=255)                                       tccTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAAtac                                                                                                                                                                                                  <  1:67567/251‑1 (MQ=255)                                        ccTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGc                                                                                                                                                                                                                 <  1:601681/235‑1 (MQ=255)                                        ccTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAAta                                                                                                                                                                                                   >  1:247458/1‑249 (MQ=255)                                         cTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAAtacta                                                                                                                                                                                                >  1:154723/1‑251 (MQ=255)                                           ttGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACg                                                                                                                                                                                              <  1:161823/251‑1 (MQ=255)                                              ggTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAAtac                                                                                                                                                                                                  >  1:889951/1‑244 (MQ=255)                                                       ccTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATc                                                                                                                                                                                  <  1:455984/251‑1 (MQ=255)                                                        cTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCa                                                                                                                                                                                 <  1:839549/251‑1 (MQ=255)                                                        cTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCa                                                                                                                                                                                 <  1:42949/251‑1 (MQ=255)                                                                  cGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGaaa                                                                                                                                                                       <  1:271558/251‑1 (MQ=255)                                                                        cTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCa                                                                                                                                                                                 >  1:10306/1‑235 (MQ=255)                                                                                           cgcACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAacac                                                                                                                                              <  1:849311/251‑1 (MQ=255)                                                                                                  aaaTTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAag                                                                                                                                       <  1:614143/251‑1 (MQ=255)                                                                                                     ttaattaaCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTc                                                                                                                                                                                                       <  1:73386/184‑1 (MQ=255)                                                                                                     ttaattaaCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAagc                                                                                                                                                                     >  1:321408/1‑218 (MQ=255)                                                                                                     ttaattaaCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAAtt                                                                                                                                                         <  1:209508/230‑1 (MQ=255)                                                                                                     ttaattaaCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAgaga                                                                                                                                    >  1:319406/1‑251 (MQ=255)                                                                                                     ttaattaaCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAgaga                                                                                                                                    >  1:457833/1‑251 (MQ=255)                                                                                                       aattaaCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAaca                                                                                                                                               >  1:88018/1‑238 (MQ=255)                                                                                                           aaCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCa                                                                                                                                                                                 <  1:353675/200‑1 (MQ=255)                                                                                                            aCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCa                                                                                                                                                                                                                              <  1:819760/154‑1 (MQ=255)                                                                                                            aCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAAtacta                                                                                                                                                                                                >  1:480586/1‑184 (MQ=255)                                                                                                            aCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAAtattat                                                                                                                                                                                   >  1:428869/1‑197 (MQ=255)                                                                                                            aCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCa                                                                                                                                                                                 <  1:342013/199‑1 (MQ=255)                                                                                                            aCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAag                                                                                                                                                                               <  1:202776/201‑1 (MQ=255)                                                                                                            aCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAag                                                                                                                                                                               >  1:632190/1‑201 (MQ=255)                                                                                                            aCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGc                                                                                                                                                                            <  1:52537/204‑1 (MQ=255)                                                                                                            aCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAAtt                                                                                                                                                         >  1:431319/1‑223 (MQ=255)                                                                                                            aCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAaca                                                                                                                                               <  1:783183/233‑1 (MQ=255)                                                                                                            aCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAagag                                                                                                                                     <  1:433883/243‑1 (MQ=255)                                                                                                            aCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTaaa                                                                                                                             >  1:90387/1‑251 (MQ=255)                                                                                                            aCTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTaaa                                                                                                                             >  1:108945/1‑251 (MQ=255)                                                                                                             cTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAAtacta                                                                                                                                                                                                <  1:760787/183‑1 (MQ=255)                                                                                                             cTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAAt                                                                                                                                                                                                    <  1:284143/179‑1 (MQ=255)                                                                                                             cTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCa                                                                                                                                                                                 <  1:753304/198‑1 (MQ=255)                                                                                                             cTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAg                                                                                                                            >  1:801809/1‑251 (MQ=255)                                                                                                               atatGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAaataa                                                                                                                                                           >  1:528766/1‑218 (MQ=255)                                                                                                                     tGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATTCTACGAGCAATATTATCAAGAGCGGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTg                                                                                                                    <  1:83025/251‑1 (MQ=255)                                                                                                                           cAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAAt                                                                                                               <  1:875823/250‑1 (MQ=255)                                                                                                                           cAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAAt                                                                                                               <  1:279282/250‑1 (MQ=255)                                                                                                                                                ccTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTAAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCa                                                                                          >  1:452380/1‑250 (MQ=255)                                                                                                                                                    aGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTg                                                                                                                          >  1:103059/1‑214 (MQ=255)                                                                                                                                                     gCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCa                                                                                                                                                                                 >  1:630278/1‑158 (MQ=255)                                                                                                                                                     gCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACttttt                                                                                    >  1:79800/1‑251 (MQ=255)                                                                                                                                                             cTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGa                                                                            >  1:125961/1‑251 (MQ=255)                                                                                                                                                                tCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAg                                                                         <  1:804501/251‑1 (MQ=255)                                                                                                                                                                     ccGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCta                                                                    >  1:482291/1‑251 (MQ=255)                                                                                                                                                                        cTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCtaaga                                                                 <  1:80541/251‑1 (MQ=255)                                                                                                                                                                            ccTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCtaagataag                                                             >  1:369933/1‑251 (MQ=255)                                                                                                                                                                                    cGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCTAAGATAAGAAAaataa                                                     <  1:90990/251‑1 (MQ=255)                                                                                                                                                                                    cGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCTAAGATAAGAAAaat                                                       <  1:700522/249‑1 (MQ=255)                                                                                                                                                                                       aaaaaGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCTAAGATAAGAAAAATAATAc                                                  <  1:296774/251‑1 (MQ=255)                                                                                                                                                                                          aaGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCTAAGATAAGAAAaataa                                                     <  1:884749/245‑1 (MQ=255)                                                                                                                                                                                               ttACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCTAAGATAAGAAAAATAATACGCAAAAAg                                          <  1:50650/251‑1 (MQ=255)                                                                                                                                                                                                 aCCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCTAAGATAAGAAAAATAATACGCAAAAAGga                                        >  1:413507/1‑251 (MQ=255)                                                                                                                                                                                                     aaagaaaTCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCTAAGATAAGAAAAATAATACGCAAAAAgg                                         <  1:430225/246‑1 (MQ=255)                                                                                                                                                                                                          aaTCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCTAAGATAAGAAAAATAATACGCAAAAAGGAGATTTTATg                               <  1:773724/251‑1 (MQ=255)                                                                                                                                                                                                          aaTCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCTAAGATAAGAAAAATAATACGCAAAAAGGAGATTTTATg                               <  1:578154/251‑1 (MQ=255)                                                                                                                                                                                                            tCACTGAAGATTCGGCGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCTAAGATAAGAAAAATAATACGCAAAAAGGAGATTTTATGt                              >  1:285898/1‑250 (MQ=255)                                                                                                                                                                                                                           cGATTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCtaaga                                                                 <  1:483135/200‑1 (MQ=255)                                                                                                                                                                                                                             aTTTACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCTAAGATAAGAAAAATAATACGCAAAAAGGAGATTTTATGTCATCCCACATTTCAGACa            <  1:796029/251‑1 (MQ=255)                                                                                                                                                                                                                               ttACCCATCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCTAAGATAAGAAAAATAATACGCAAAAAGGAGAtttt                                  <  1:595117/227‑1 (MQ=255)                                                                                                                                                                                                                                      tCTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCTAAGATAAGAAAAATAATACGCAAAAAGGAGATTTTATGTCATCCCACATTTCAGACAAGATTCAAg   <  1:380218/251‑1 (MQ=255)                                                                                                                                                                                                                                       cTGCCGACGTGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCTAAGATAAGAAAAATAATACGCAAAAAGGAGATTTTATGTCATCCCACATTTCAGACAAGATTCAAGa  <  1:783485/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   GGCACCTTTAGACATGGTGTTCCCGAAAGAAGGTCCGGTCCTTTGGGTAGATACCCTCGCGATTCCGGTTACTTCTAAAAACCCGGACGGCGCACACAAATTAATTAACTATATGTTGGGCGCAAAAGCGGCGGAAAAATTAACCTTAGCTATCGGCTATCCAACCGCTAACCTTGAAGCGAAAAAAGCCTTACCGAAAGAAATCACTGAAGATTCGGCGATTTACCCATCTGCCGACATGCTACAAAAAAGCTACTGGCAAGATGATGTGGGCGACGCGATTCAATACTACGAGCAATATTATCAAGAGCTGAAAGCAGCGAAATAATTAGTTCCAACACAAAAAAGAGAAGCTAAAGTGCGGTTGAAAATTCATGTAAAATTTCGACCGCACTTTTTTATTGTGAAAGTGCTAAGATAAGAAAAATAATACGCAAAAAGGAGATTTTATGTCATCCCACATTTCAGACAAGATTCAAGA  >  1/1910039‑1910519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑