Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,897,400	A→G	V161A (GTG→GCG)	unknown ←	L,D‑transpeptidase YcbB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,897,400	0	A	G	100.0%	194.1 / NA	68	V161A (GTG→GCG)	unknown	L,D‑transpeptidase YcbB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/33);  total (35/33)

ATACCGTTTTTATCTAATTTGCCGTTTAGGATTAAATTTTCTACCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACACATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTATTTTCCGCATAAATTTTTGCGATTAACGGTTTAAATTGCAGTTCCGTTTCACCGATGCGTTCAGCTAAACCTT  >  1/1897154‑1897640                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 aTACCGTTTTTATCTAATTTGCCGTTTAGGATTAAATTTTCTACCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCAt                                                                                                                                                                                                                                               >  1:613731/1‑250 (MQ=255) aTACCGTTTTTATCTAATTTGCCGTTTAGGATTAAATTTTCTACCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCAt                                                                                                                                                                                                                                               >  1:467651/1‑250 (MQ=255)     cGTTTTTATCTAATTTGCCGTTTAGGATTAAATTTTCTACCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTc                                                                                                                                                                                                                                          <  1:482724/251‑1 (MQ=255)          ttATCTAATTTGCCGTTTAGGATTAAATTTTCTACCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGAt                                                                                                                                                                                                                                     <  1:820383/251‑1 (MQ=255)          ttATCTAATTTGCCGTTTAGGATTAAATTTTCTACCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGAt                                                                                                                                                                                                                                     <  1:528500/251‑1 (MQ=255)          ttATCTAATTTGCCGTTTAGGATTAAATTTTCTACCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGAt                                                                                                                                                                                                                                     <  1:395293/251‑1 (MQ=255)             tCTAATTTGCCGTTTAGGATTAAATTTTCTACCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAAc                                                                                                                                                                                                                                  <  1:837139/251‑1 (MQ=255)               tAATTTGCCGTTTAGGATTAAATTTTCTACCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACAt                                                                                                                                                                                                                                <  1:583801/251‑1 (MQ=255)               tAATTTGCCGTTTAGGATTAAATTTTCTACCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACAt                                                                                                                                                                                                                                <  1:531329/251‑1 (MQ=255)                aaTTTGCCGTTTAGGATTAAATTTTCTACCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATc                                                                                                                                                                                                                               <  1:324878/251‑1 (MQ=255)                      ccGTTTAGGATTAAATTTTCTACCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGcac                                                                                                                                                                                                                         <  1:324666/251‑1 (MQ=255)                        gTTTAGGATTAAATTTTCTACCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACAt                                                                                                                                                                                                                                >  1:635422/1‑242 (MQ=255)                                     ttttCTACCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTAt                                                                                                                                                                                                           >  1:574582/1‑250 (MQ=255)                                        tCTACCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGc                                                                                                                                                                                                       <  1:138002/251‑1 (MQ=255)                                           aCCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGa                                                                                                                                                                                                    >  1:598795/1‑251 (MQ=255)                                               tGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTgg                                                                                                                                                                                                >  1:219401/1‑251 (MQ=255)                                                ggCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTgg                                                                                                                                                                                                >  1:462745/1‑250 (MQ=255)                                                      cGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTAt                                                                                                                                                                                                           <  1:251688/233‑1 (MQ=255)                                                      cGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTg                                                                                                                                                                                         <  1:791484/251‑1 (MQ=255)                                                      cGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTg                                                                                                                                                                                         <  1:704008/251‑1 (MQ=255)                                                           gCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGcac                                                                                                                                                                                                                         >  1:382018/1‑214 (MQ=255)                                                           gCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTg                                                                                                                                                                                         <  1:585669/246‑1 (MQ=255)                                                                aataatGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAg                                                                                                                                                                                 >  1:274898/1‑249 (MQ=255)                                                                aataatGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGAc                                                                                                                                                                               <  1:463838/251‑1 (MQ=255)                                                                               ccGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGcac                                                                                                                                                                                                                         >  1:447394/1‑194 (MQ=255)                                                                               ccGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGcac                                                                                                                                                                                                                         >  1:14914/1‑194 (MQ=255)                                                                               ccGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCtt                                                                                                                                                                >  1:792725/1‑251 (MQ=255)                                                                                  gATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAg                                                                                                                                                                       <  1:415321/241‑1 (MQ=255)                                                                                       gTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGAc                                                                                                                                                                               <  1:159574/228‑1 (MQ=255)                                                                                       gTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATg                                                                                                                                                         >  1:473536/1‑250 (MQ=255)                                                                                       gTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATg                                                                                                                                                         >  1:521688/1‑250 (MQ=255)                                                                                                         aaCATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACg                                                                                                                                      <  1:645765/251‑1 (MQ=255)                                                                                                         aaCATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACg                                                                                                                                      <  1:644380/251‑1 (MQ=255)                                                                                                         aaCATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACg                                                                                                                                      <  1:257277/251‑1 (MQ=255)                                                                                                         aaCATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACg                                                                                                                                      <  1:710293/251‑1 (MQ=255)                                                                                                                ttattTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATc                                                                                                                                                                                                          >  1:767804/1‑176 (MQ=255)                                                                                                                ttattTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATc                                                                                                                               <  1:375564/251‑1 (MQ=255)                                                                                                                  attTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCa                                                                                                                                    >  1:625374/1‑244 (MQ=255)                                                                                                                  attTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCa                                                                                                                              >  1:670103/1‑250 (MQ=255)                                                                                                                     tttGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCa                                                                                                                          <  1:623703/251‑1 (MQ=255)                                                                                                                                        gAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAAc                                                                                                                                       >  1:175373/1‑219 (MQ=255)                                                                                                                                             gACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCAt                                                                                                  >  1:863407/1‑251 (MQ=255)                                                                                                                                                    ttttCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGcc                                                                                            <  1:549958/250‑1 (MQ=255)                                                                                                                                                             ggTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCg                                                                                                                                                      >  1:363616/1‑183 (MQ=255)                                                                                                                                                                      gCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAattatt                                                                         >  1:611055/1‑251 (MQ=255)                                                                                                                                                                      gCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAattatt                                                                         >  1:517314/1‑251 (MQ=255)                                                                                                                                                                       cTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTAttt                                                                        <  1:421862/251‑1 (MQ=255)                                                                                                                                                                         ttGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTATTTTc                                                                      <  1:330368/251‑1 (MQ=255)                                                                                                                                                                           gTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGCTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTATTTTCCg                                                                    >  1:587944/1‑251 (MQ=255)                                                                                                                                                                                        gAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTAttt                                                                        >  1:839717/1‑234 (MQ=255)                                                                                                                                                                                          atatAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAAt                                                                                                                                <  1:663985/176‑1 (MQ=255)                                                                                                                                                                                           tataACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGa                                                                                                                                                             >  1:252640/1‑146 (MQ=255)                                                                                                                                                                                            ataACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTATTTTCCGCATAAATTTTTGCGAtt                                                   >  1:38/1‑251 (MQ=255)                                                                                                                                                                                                         cgcAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTATTTTCCGCATAAATTTTTGCGATTAACGGTTTAAAt                                       <  1:751930/250‑1 (MQ=255)                                                                                                                                                                                                               tttCTTCACATTATTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACtgttg                                                                                                                >  1:758301/1‑171 (MQ=255)                                                                                                                                                                                                               tttCATCACATTAGTGGCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAAt                                                                                                                                >  1:636676/1‑155 (MQ=255)                                                                                                                                                                                                                               gCATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAAc                                                                                                                     <  1:355111/150‑1 (MQ=255)                                                                                                                                                                                                                                cATAAAACAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTATTTTCCGc                                                                   >  1:883942/1‑199 (MQ=255)                                                                                                                                                                                                                                    aaaCAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTATTTTCCGCATAAATTTTTGCGATTAACGGTTTAAATTGCAGTTCCGTTTCACCGATGCGTTCAg           >  1:798843/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaCAGATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTATTTTCCGCATAAATTTTTGCGATTAACGGTTTAAATTGCAGTTCCGTTTCACCGATGCGTTCAGc          <  1:776033/251‑1 (MQ=255)                                                                                                                                                                                                                                         gATAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTAttt                                                                        >  1:340958/1‑185 (MQ=255)                                                                                                                                                                                                                                          aTAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTATTTTCCGCATAAATTTTTGCGATTAACGGTTTAAATTGCAGTTCCGTTTCACCGATGCGTTCAGCTAAAc     <  1:720776/251‑1 (MQ=255)                                                                                                                                                                                                                                          aTAATCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTATTTTCCGCATAAATTTTTGCGATTAACGGTTTAAATTGCAGTTCCGTTTCACCGATGCGTTCAGCTAAAc     <  1:718543/251‑1 (MQ=255)                                                                                                                                                                                                                                           tAATCTAAGAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTATTTTCCGCATAAATTTTTGCGATTAACGGTTTAAATTGCAGTTCCGTTTCACCGATGCGTTCAGCTAAAcc    <  1:354365/251‑1 (MQ=255)                                                                                                                                                                                                                                             aTCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTATTTTCCGCATAAATTTTTGCGATTAACGGTTTAAATTGCAGTTCCGTTTCACCGATGCGTTCAGCTAAAc     >  1:463383/1‑248 (MQ=255)                                                                                                                                                                                                                                             aTCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTATTTTCCGCATAAATTTTTGCGATTAACGGTTTAAATTGCAGTTCCGTTTCACCGATGCGTTCAGCTAAACCtt  >  1:268224/1‑251 (MQ=255)                                                                                                                                                                                                                                             aTCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTATTTTCCGCATAAATTTTTGCGATTAACGGTTTAAATTGCAGTTCCGTTTCACCGATGCGTTCAGCTAAACCtt  >  1:47838/1‑251 (MQ=255)                                                                                                                                                                                                                                             aTCTAAAAACGCATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTATTTTCCGCATAAATTTTTGCGATTAACGGTTTAAATTGCAGTTCCGTTTCACCGATGCGTTCAGCTAAACCtt  >  1:128284/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 ATACCGTTTTTATCTAATTTGCCGTTTAGGATTAAATTTTCTACCTTGGCTAACGTCTGCTTAAATAATGGATTTTCACCGGATAAGTTATTCACGAAAGCAAAAACATCATTATTTTTGACCGCACTTAGCCAGGAATCGACCTGGTTTTCGTCCGGTTTACCTGCTTTGTAGCTATTTTCCGAATATAACCATTTTTGCGCAAATTTCTTCACATTATTGGCATAAAACAGATAATCTAAAAACACATCGGTCAAGATAACATCTTGCACAAATGCTTCTTTATCGGCAGACTGGCTTACTGCATTTAAGACGTTGGCAGAACGCTTGGAAATGCCGCTTGCCACCATTAACGCATAATCACGCAAGAACTGTTGTTTGGCTTCTTCATCCTGCCACAGCGGCATAAAATTATTTTCCGCATAAATTTTTGCGATTAACGGTTTAAATTGCAGTTCCGTTTCACCGATGCGTTCAGCTAAACCTT  >  1/1897154‑1897640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑