Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,863,196	T→C	A9A (GCA→GCG)	unknown ←	Low‑affinity gluconate/H+ symporter GntU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,863,196	0	T	C	100.0%	55.1 / NA	21	A9A (GCA→GCG)	unknown	Low‑affinity gluconate/H+ symporter GntU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/15);  total (6/15)

TTTATTAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGATCATGGTACCCAAACCGACTAATAACGCCACGGACGCCAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACTGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATGTTGATAATGTTATCGGTAACATACCTTTTTGCCGACTTTTGGTAAATAAACAGCATTTAAAAATGTGATCACAATCACAAAAAATTATATTTATGCGACATATTCAGCAA  >  1/1862950‑1863346                                                                                                                                                                                                                                                       |                                                                                                                                                       tttATTAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGATCATGGTACCCAAACCGACTAATAACGCCACGGACGCCAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCAccgctg                                                                                                                                                    <  1:655516/251‑1 (MQ=255)                  ccGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGATCATGGTACCCAAACCGACTAATAACGCCACGGACGCCAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAAtt                                                                                                                                   <  1:811343/251‑1 (MQ=255)                     cAAGCACTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGATCATGGTACCCAAACCGACTAATAACGCCACGGACGCCAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAAc                                                                                                                                <  1:559840/251‑1 (MQ=255)                               tGCCCCGCCGGTGACTTCCAATAAACGCCCGATCATGGTACCCAAACCGACTAATAACGCCACGGACGCCAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTc                                                                                                                      <  1:58148/251‑1 (MQ=255)                                     gccgGTGACTTCCAATAAACGCCCGATCATGGTACCCAAACCGACTAATAACGCCACGGACGCCAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTc                                                                                                                      >  1:439170/1‑245 (MQ=255)                                        ggTGACTTCCAATAAACGCCCGATCATGGTACCCAAACCGACTAATAACGCCACGGACGCCAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATc                                                                                                                                               >  1:629518/1‑217 (MQ=255)                                             cTTCCAATAAACGCCCGATCATGGTACCCAAACCGACTAATAACGCCACGGACGCCAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATaaaaa                                                                                                                                     <  1:399086/222‑1 (MQ=255)                                               tCCAATAAACGCCCGATCATGGTACCCAAACCGACTAATAACGCCACGGACGCCAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATGTTGATAATGt                                                                                                      <  1:86631/251‑1 (MQ=255)                                                ccAATAAACGCCCGATCATGGTACCCAAACCGACTAATAACGCCACGGACGCCAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATg                                                                                                                <  1:596139/240‑1 (MQ=255)                                                                      aCCCAAACCGACTAATAACGCCACGGACGCCAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATGTTGATAATGTTATCGGTAACATAcc                                                                                       >  1:405260/1‑243 (MQ=255)                                                                                 cTAATAACGCCACGGACGCCAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATGTTGATAATGTTATCGGTAACATACCTTTTTGCCGACTTTTGGta                                                                    <  1:834837/251‑1 (MQ=255)                                                                                 cTAATAACGCCACGGACGCCAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATGTTGATAATGTTATCGGTAACATACCTTTTTGCCGACTTTTGGta                                                                    <  1:733300/251‑1 (MQ=255)                                                                                        cGCCACGGACGCCAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATGTTGATAATGTTATCGGTAACATACCTTTTTGCCGACTTTTGGtaaataaa                                                              >  1:820319/1‑250 (MQ=255)                                                                                                 cGCCAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATGTTGATAATGTTATCgg                                                                                                >  1:774110/1‑207 (MQ=255)                                                                                                   ccAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATGt                                                                                                               >  1:145807/1‑190 (MQ=255)                                                                                                   ccAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATGTTGATAATGTTATCGGTAACATACCTTTTTGCCGACTTTTGGTAAATAAACAGCATTTaaa                                                   <  1:142346/250‑1 (MQ=255)                                                                                                        gtgtTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATGTTGATAATGTTATCGGTAACATACCttt                                                                                    <  1:98230/212‑1 (MQ=255)                                                                                                        gtgtTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATGTTGATAATGTTATCGGTAACATACCTTTTTGCCGACTTTTGGTAAATAAACAGCATTTAAAAAtg                                               <  1:409055/249‑1 (MQ=255)                                                                                                                                 tCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATGTTGATAATGTTATCGGTAACATACCTTTTTGCCGACTTTTGGTAAATAAACAGCATTTAAAAATGTGATCACAATCACAAAAAATTATAttt                    <  1:784637/251‑1 (MQ=255)                                                                                                                                         aTTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATGTTGATAATGTTATCGGTAACATACCttt                                                                                    <  1:302928/179‑1 (MQ=255)                                                                                                                                                   cGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATGTTGATAATGTTATCGGTAACATACCTTTTTGCCGACTTTTGGTAAATAAACAGCATTTAAAAATGTGATCACAATCACAAAAAATTATATTTATGCGACATATTCAGCaa  <  1:750850/251‑1 (MQ=255)                                                                                                                                                                                                                                            aGTAAAAGCACCGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATGTTGATAATGTTATCGGTAACATACCTTTTTGCCGACTTTTGGTAAATAAACAGCATTTAAAAATGTGATCACAATCACAAAGAATTATATTTATGCGACATATTc       <  1:425114/157‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                       TTTATTAATCAACGTATCCGCAAGCACTTTTGCCCCGCCGGTGACTTCCAATAAACGCCCGATCATGGTACCCAAACCGACTAATAACGCCACGGACGCCAAGGTGTTACCGAAGCCGGTTAACATGGTCGGCAGGATTTTATCCACGGCAATTCCTGCGGCAAGTGCGGTCAATAAACTGACTAAAATTAACGCCACAAAGGCATGCATTTTAAATTTGATAATTAATAGCAGAAGTAAAAGCACTGCTGCGATCATAATAAAAATTAACATAATAATTCCTTATGTTGATAATGTTATCGGTAACATACCTTTTTGCCGACTTTTGGTAAATAAACAGCATTTAAAAATGTGATCACAATCACAAAAAATTATATTTATGCGACATATTCAGCAA  >  1/1862950‑1863346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑