Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,862,352	T→C	S291G (AGT→GGT)	unknown ←	Low‑affinity gluconate/H+ symporter GntU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,862,352	0	T	C	100.0%	181.4 / NA	64	S291G (AGT→GGT)	unknown	Low‑affinity gluconate/H+ symporter GntU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/34);  total (30/34)

GCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATAATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCACGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACTGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAACCCGGTATCAAACAAAATAAGGAAAATCGGTAAAACCAAAATAAACAGAACGCGCCCAAACGCCGGTGGATTTTGCACGGAGGTTTCGCTGATGGCGGCGGTGTT  >  1/1862120‑1862592                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 gCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTtctc                                                                                                                                                                                                                                          <  1:790307/241‑1 (MQ=255)     tagtCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATTCCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAggc                                                                                                                                                                                                                             <  1:653001/250‑1 (MQ=255)     tagtCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAggc                                                                                                                                                                                                                             <  1:728649/250‑1 (MQ=255)     tagtCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAgg                                                                                                                                                                                                                              <  1:852040/249‑1 (MQ=255)           gggCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAggcaatggca                                                                                                                                                                                                                      >  1:724236/1‑251 (MQ=255)           gggCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAggcaatggca                                                                                                                                                                                                                      >  1:774600/1‑251 (MQ=255)             gCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAggcaatggcaa                                                                                                                                                                                                                     <  1:316394/250‑1 (MQ=255)                 ccGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATtaat                                                                                                                                                                                                                <  1:292717/251‑1 (MQ=255)                    ttGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATtaat                                                                                                                                                                                                                >  1:186713/1‑248 (MQ=255)                     tGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAAtg                                                                                                                                                                                                            <  1:643324/251‑1 (MQ=255)                            ccTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATtaa                                                                                                                                                                                                     >  1:531054/1‑251 (MQ=255)                             cTTGTGCCCCACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATtaat                                                                                                                                                                                                    <  1:814531/251‑1 (MQ=255)                             cTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATtaat                                                                                                                                                                                                    <  1:680437/251‑1 (MQ=255)                                 tgCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAAcg                                                                                                                                                                                                <  1:297730/251‑1 (MQ=255)                                  gCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATtaataa                                                                                                                                                                                                  <  1:762866/248‑1 (MQ=255)                                            aTGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAggc                                                                                                                                                                                                                             >  1:190113/1‑211 (MQ=255)                                              gCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGtttt                                                                                                                                                                                   >  1:394982/1‑251 (MQ=255)                                                        taataaAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAAcg                                                                                                                                                                                                >  1:174603/1‑228 (MQ=255)                                                        taataaAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAgg                                                                                                                                                                         >  1:363312/1‑251 (MQ=255)                                                        taataaAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAgg                                                                                                                                                                         >  1:539705/1‑251 (MQ=255)                                                        taataaAGGCGGCGATGATGATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAgg                                                                                                                                                                         >  1:41383/1‑251 (MQ=255)                                                                         atgaTCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCca                                                                                                                                                        >  1:557953/4‑251 (MQ=255)                                                                             tCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACCAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTATTAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAg                                                                                                                                                    >  1:95358/1‑251 (MQ=255)                                                                                gCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAAtg                                                                                                                                                                                                            <  1:519978/192‑1 (MQ=255)                                                                                gCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAg                                                                                                                                                    <  1:356696/248‑1 (MQ=255)                                                                                    aCCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAAc                                                                                                                                             <  1:763738/251‑1 (MQ=255)                                                                                     ccGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAAc                                                                                                                                             >  1:687545/1‑250 (MQ=255)                                                                                        gTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCg                                                                                                                                           <  1:72457/249‑1 (MQ=255)                                                                                               acaacaTGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCAc                                                                                                                                   <  1:14592/250‑1 (MQ=255)                                                                                                caacaTGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCt                                                                                                                                                                 <  1:577074/219‑1 (MQ=255)                                                                                                   caTGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTg                                                                                                                              >  1:708898/1‑251 (MQ=255)                                                                                                     tGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGcc                                                                                                                            <  1:176464/251‑1 (MQ=255)                                                                                                       gCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAggcaa                                                                                                                                                                                                                           <  1:84871/154‑1 (MQ=255)                                                                                                       gCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAg                                                                                                                                                    >  1:817060/1‑225 (MQ=255)                                                                                                       gCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCa                                                                                                                           <  1:605796/250‑1 (MQ=255)                                                                                                              aCTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAgg                                                                                                                   >  1:567337/1‑251 (MQ=255)                                                                                                               cTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAggg                                                                                                                  <  1:305097/251‑1 (MQ=255)                                                                                                                tCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGt                                                                                                                 <  1:730994/251‑1 (MQ=255)                                                                                                                       cGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAAc                                                                                                          <  1:86899/251‑1 (MQ=255)                                                                                                                               cTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAACCCGGTAt                                                                                                   >  1:192048/1‑250 (MQ=255)                                                                                                                                       cAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTtc                                                                                                                                                                                                                                            >  1:446264/1‑105 (MQ=255)                                                                                                                                       cAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGa                                                                                                                                                                                             <  1:620007/152‑1 (MQ=255)                                                                                                                                         aTACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATta                                                                                                                                                                                                      <  1:702352/141‑1 (MQ=255)                                                                                                                                                           cgcccgcACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATc                                                                                                                                     <  1:239940/188‑1 (MQ=255)                                                                                                                                                                    cGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAACCCGGTATCAAACAAAATAAGGAAAATCGGTAAAACCAAAATAAAc                                                             <  1:437662/251‑1 (MQ=255)                                                                                                                                                                       tCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAACCCGGTATCAAACAAAATAAGGAAAATCGGTAAAACCAAAATAAACAg                                                           <  1:613492/250‑1 (MQ=255)                                                                                                                                                                          cTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAACCCGGTATCAAACAAAATAAGGAAAATCGGTAAAACCAAAATAAACAGAAcg                                                       >  1:241978/1‑251 (MQ=255)                                                                                                                                                                              aataatGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGCCAATCACTTTTGCCACGCTGAGGGCATTTAACCCGGTATCAAACAAAATAAGGAAAATCGGTAAAACCAAAATAAAc                                                             >  1:529818/1‑241 (MQ=255)                                                                                                                                                                                taatGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAACCCGGTATCAAACAAAATAAGGAAAATCGGTAAAACCAAAATAAACAGAACGCGCCCa                                                 >  1:101670/1‑251 (MQ=255)                                                                                                                                                                                               gTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAACCCGGTATCAAACAAAATAAGGAAAATCGGTAAAACCAAAATAAAc                                                             <  1:550024/224‑1 (MQ=255)                                                                                                                                                                                                tCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGc                                                                                                                                                                  <  1:845415/122‑1 (MQ=255)                                                                                                                                                                                                  ccAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAAcc                                                                                                         >  1:866807/1‑177 (MQ=255)                                                                                                                                                                                                          cTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAggcaatggc                                                                                                                                                                                                                       <  1:265900/59‑1 (MQ=255)                                                                                                                                                                                                          cTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGc                                                                                                                                                                                 >  1:534802/1‑97 (MQ=255)                                                                                                                                                                                                            gTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATACTGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAACCCGGCATCAAACAAAATAAGGAAAATCGGTAAAACCAAAATAAACAGAACGCGCCCAAACGCCGGTGGATTTTGCACGGAGGttt                     >  1:406053/1‑251 (MQ=255)                                                                                                                                                                                                            gTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAACCCGGTATCAAACAAAAt                                                                                         <  1:803093/183‑1 (MQ=255)                                                                                                                                                                                                            gTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAACCCGGTATCAAACAAAATAAGGAAAATCGGTAAAACCAAAATAAACAGAACGCGCCCAAACGCCGGTGGATTTTGCACGGAGGttt                     >  1:612683/1‑251 (MQ=255)                                                                                                                                                                                                            gTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAACCCGGTATCAAACAAAATAAGGAAAATCGGTAAAACCAAAATAAACAGAACGCGCCCAAACGCCGGTGGATTTTGCACGGAGGttt                     >  1:16871/1‑251 (MQ=255)                                                                                                                                                                                                            gTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAACCCGGTATCAAACAAAATAAGGAAAATCGGTAAAACCAAAATAAACAGAACGCGCCCAAACGCCGGTGGATTTTGCACGGAGGttt                     >  1:276324/1‑251 (MQ=255)                                                                                                                                                                                                              tACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAggc                                                                                                                                                                                                                             <  1:551414/49‑1 (MQ=255)                                                                                                                                                                                                                     gCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATtaat                                                                                                                                                                                                    >  1:318383/1‑67 (MQ=255)                                                                                                                                                                                                                          cGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAACCCGGTATCAAACAAAATAAGGAAAATCGGTAAAACCAAAATAAACAGAACGCGCCCAAACGCCGGTGGATTTTGCACGGAGGTTTCGCTGATGgcggcg       >  1:677977/1‑251 (MQ=255)                                                                                                                                                                                                                                gTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATtaat                                                                                                                                                                                                    <  1:56620/56‑1 (MQ=255)                                                                                                                                                                                                                                gTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAACCCGGTATCAAACAAAATAAGGAAAATCGGTAAAACCAAAATAAACAGAACGCGCCCAAACGCCGGTGGATTTTGCACGGAGGTTTCGCTGATGGCGGCGGTGtt  >  1:759073/1‑250 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 GCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATAATCGGCATACCGGTATTTGACAACATGGCGGATAACTCATCGCCGATGCCGCTGGCACGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACTGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGGCGCAGGCTTTCGACCCACAAGGAAGAACCGTCAATCACTTTTGCCACGCTGAGGGTATTTAACCCGGTATCAAACAAAATAAGGAAAATCGGTAAAACCAAAATAAACAGAACGCGCCCAAACGCCGGTGGATTTTGCACGGAGGTTTCGCTGATGGCGGCGGTGTT  >  1/1862120‑1862592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑