Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,862,194	A→G	I343I (ATT→ATC)	unknown ←	Low‑affinity gluconate/H+ symporter GntU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,862,194	0	A	G	100.0%	243.4 / NA	81	I343I (ATT→ATC)	unknown	Low‑affinity gluconate/H+ symporter GntU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/39);  total (42/39)

CGTGGTTTTGGTGTCCATTTCCAAGAAACGGCTTATTAACCAGAAACCGCTGTCGTTCACATGGGAGAATACGGTGGCGCCGGAGGCAATGGCAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATAATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCACGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACTGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGG  >  1/1861954‑1862425                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         cGTGGTTTTGGTGTCCATTTCCAAGAAACGGCTTATTAACCAGAAACCGCTGTCGTTCACATGGGAGAATACGGTGGCGCCGGAGGCAATGGCAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATAc                                                                                                                                                                                                                                <  1:600222/251‑1 (MQ=255)            tgtCCATTTCCAAGAAACGGCTTATTAACCAGAAACCGCTGTCGTTCACATGGGAGAATACGGTGGCGCCGGAGGCAATGGTAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGac                                                                                                                                                                                                                     <  1:428178/251‑1 (MQ=255)               ccATTTCCAAGAAACGGCTTATTAACCAGAAACCGCTGTCGTTCACATGGGAGAATACGGTGGCGCCGGAGGCAATGGCAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGaca‑ac                                                                                                                                                                                                                 <  1:244556/251‑1 (MQ=255)                         gAAACGGCTTATTAACCAGAAACCGCTGTCGTTCACATGGGAGAATACGGTGGCGCCGGAGGCAATGGCAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATa                                                                                                                                                                                                       >  1:462480/1‑251 (MQ=255)                           aaCGGCTTATTAACCAGAAACCGCTGTCGTTCACATGGGAGAATACGGTGGCGCCGGAGGCAATGGCAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAAc                                                                                                                                                                                                     <  1:781982/251‑1 (MQ=255)                             cGGCTTATTAACCAGAAACCGCTGTCGTTCACATGGGAGAATACGGTGGCGCCGGAGGCAATGGCAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTc                                                                                                                                                                                                   <  1:420773/251‑1 (MQ=255)                                                      cGTTCACATGGGAGAATACGGTGGCGCCGGAGGCAATGGCAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAAt                                                                                                                                                                          >  1:217128/1‑251 (MQ=255)                                                         tCACATGGGAGAATACGGTGGCGCCGGAGGCAATGGCAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCAGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAAc                                                                                                                                                                                                     >  1:34363/1‑221 (MQ=255)                                                           acaTGGGAGAATACGGTGGCGCCGGAGGCAATGGCAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCAt                                                                                                                                                                                                                                  >  1:847229/1‑191 (MQ=255)                                                            caTGGGAGAATACGGTGGCGCCGGAGGCAATGGCAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCa                                                                                                                                                                    >  1:287461/1‑251 (MQ=255)                                                             aTGGGAGAATACGGTGGCGCCGGAGGCAATGGCAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCgcctttattatttctatggcattgcg                                                                                                                                                                   <  1:245245/251‑26 (MQ=255)                                                              tGGGAGAATACGGTGGCGCCGGAGGCAATGGCAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCAc                                                                                                                                                                   >  1:597803/1‑250 (MQ=255)                                                                ggAGAATACGGTGGCGCCGGAGGCAATGGCAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGa                                                                                                                                                                >  1:595043/1‑251 (MQ=255)                                                                 gagaATACGGTGGCGCCGGAGGCAATGGCAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGaa                                                                                                                                                               >  1:786756/1‑251 (MQ=255)                                                                   gaATACGGTGGCGCCGGAGGCAATGGCAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACa                                                                                                                                                             >  1:257583/1‑251 (MQ=255)                                                                   gaATACGGTGGCGCCGGAGGCAATGGCAACCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACa                                                                                                                                                             >  1:329267/1‑251 (MQ=255)                                                                                           ggcaatCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGac                                                                                                                                                                                                                     >  1:813198/1‑172 (MQ=255)                                                                                              aatCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAaataa                                                                                                                                  <  1:707340/251‑1 (MQ=255)                                                                                                 cACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATgg                                                                                                                               <  1:310198/251‑1 (MQ=255)                                                                                                   cGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATAc                                                                                                                                                                        <  1:697262/208‑1 (MQ=255)                                                                                                   cGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAg                                                                                                                             <  1:65384/251‑1 (MQ=255)                                                                                                     aTAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCa                                                                                                                           >  1:602145/1‑251 (MQ=255)                                                                                                          aCATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCg                                                                                                                      >  1:378848/1‑251 (MQ=255)                                                                                                          aCATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCg                                                                                                                      >  1:845416/1‑251 (MQ=255)                                                                                                                aTCCCATTGGCTGAGATCGGTTGCGTCTTCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCTAACCTTGTGCCACACGCAACGCCATAGAAATAATAAAGGCGGCGACCCTGATCGGCACACCGTTCTTTTACACACAT‑TCTTATCACTCATCCCCGATGCCGCTTGCGCGCATTACGCCACCTAACATACCGGCCGCACCGGTc                                                                                                                                            >  1:190387/1‑223 (MQ=255)                                                                                                                        ggCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATAccgcc                                                                                                                                                      >  1:734655/1‑205 (MQ=255)                                                                                                                         gCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAAc                                                                                                                                                              <  1:788346/196‑1 (MQ=255)                                                                                                                         gCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATgg                                                                                                                               >  1:266700/1‑227 (MQ=255)                                                                                                                            gagaTCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAaataa                                                                                                                                  >  1:764854/1‑221 (MQ=255)                                                                                                                            gagaTCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTa                                                                                                     >  1:515456/1‑250 (MQ=255)                                                                                                                               aTCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACaa                                                                                                  <  1:778140/250‑1 (MQ=255)                                                                                                                                   gTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGc                                                                                              <  1:657234/250‑1 (MQ=255)                                                                                                                                            gcAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCAc                                                                                                                                                 >  1:65908/1‑190 (MQ=255)                                                                                                                                                    cGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAAc                                                                            <  1:866530/251‑1 (MQ=255)                                                                                                                                                    cGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAAc                                                                            <  1:836749/251‑1 (MQ=255)                                                                                                                                                    cGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATGTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAAc                                                                            <  1:835128/251‑1 (MQ=255)                                                                                                                                                     ggggCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAAc                                                                            >  1:575997/1‑250 (MQ=255)                                                                                                                                                           aTTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAAc                                                                                                                                                              <  1:268094/162‑1 (MQ=255)                                                                                                                                                                       gCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTtctc                                                                   <  1:790307/241‑1 (MQ=255)                                                                                                                                                                           tagtCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATTCCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAggc                                                      <  1:653001/250‑1 (MQ=255)                                                                                                                                                                           tagtCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAggc                                                      <  1:728649/250‑1 (MQ=255)                                                                                                                                                                           tagtCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAgg                                                       <  1:852040/249‑1 (MQ=255)                                                                                                                                                                            agtCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGac                                                                                                                                                                                                                     <  1:426882/91‑1 (MQ=255)                                                                                                                                                                            agtCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTc                                                                                 >  1:782381/1‑222 (MQ=255)                                                                                                                                                                            agtCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAAc                                                                            <  1:760309/227‑1 (MQ=255)                                                                                                                                                                                 gggCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATATAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATc                                                                                                                       <  1:410097/179‑1 (MQ=255)                                                                                                                                                                                 gggCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGaca‑a                                                                                                                                                                                                                  >  1:132857/1‑88 (MQ=255)                                                                                                                                                                                 gggCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAgg                                                                                               >  1:609766/1‑203 (MQ=255)                                                                                                                                                                                 gggCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAggcaatggca                                               >  1:774600/1‑251 (MQ=255)                                                                                                                                                                                 gggCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAggcaatggca                                               >  1:724236/1‑251 (MQ=255)                                                                                                                                                                                   gCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAggcaatggcaa                                              <  1:316394/250‑1 (MQ=255)                                                                                                                                                                                      aCCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAaat                                                                                                                                    <  1:498629/161‑1 (MQ=255)                                                                                                                                                                                       ccGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATtaat                                         <  1:292717/251‑1 (MQ=255)                                                                                                                                                                                          ttGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTcc                                                                                                                  >  1:833767/1‑175 (MQ=255)                                                                                                                                                                                          ttGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAAc                                                                            >  1:540885/1‑213 (MQ=255)                                                                                                                                                                                          ttGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATtaat                                         >  1:186713/1‑248 (MQ=255)                                                                                                                                                                                           tGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAAtg                                     <  1:643324/251‑1 (MQ=255)                                                                                                                                                                                            gCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAgg                                                                                               <  1:204952/192‑1 (MQ=255)                                                                                                                                                                                                  ccTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATtaa                              >  1:531054/1‑251 (MQ=255)                                                                                                                                                                                                   cTTGTGCCCCACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATtaat                             <  1:814531/251‑1 (MQ=255)                                                                                                                                                                                                   cTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAgcgc                                                                                                            <  1:606218/172‑1 (MQ=255)                                                                                                                                                                                                   cTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATtaat                             <  1:680437/251‑1 (MQ=255)                                                                                                                                                                                                      gtgCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTaa                                                                                                                                       >  1:232677/1‑142 (MQ=255)                                                                                                                                                                                                      gtgCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTccc                                                                                                                 <  1:413026/164‑1 (MQ=255)                                                                                                                                                                                                       tgCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAAcg                         <  1:297730/251‑1 (MQ=255)                                                                                                                                                                                                        gCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATtaataa                           <  1:762866/248‑1 (MQ=255)                                                                                                                                                                                                             acGCAATGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAgcg                                                                                                             <  1:735883/161‑1 (MQ=255)                                                                                                                                                                                                                  aTGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAgg                                                                                               >  1:500913/1‑170 (MQ=255)                                                                                                                                                                                                                  aTGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAg                                                                                                >  1:509519/1‑169 (MQ=255)                                                                                                                                                                                                                  aTGCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAggc                                                      >  1:190113/1‑211 (MQ=255)                                                                                                                                                                                                                    gCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAaat                                                                                                                                    >  1:88623/1‑131 (MQ=255)                                                                                                                                                                                                                    gCCATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGtttt            >  1:394982/1‑251 (MQ=255)                                                                                                                                                                                                                     ccATAGAAATAATAAAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATgg                                                                                                                               >  1:290550/1‑135 (MQ=255)                                                                                                                                                                                                                              taataaAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGc                                                                                                                                                                                                            <  1:543235/49‑1 (MQ=255)                                                                                                                                                                                                                              taataaAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATgg                                                                                                                               >  1:773200/1‑126 (MQ=255)                                                                                                                                                                                                                              taataaAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAgcg                                                                                                             <  1:835428/144‑1 (MQ=255)                                                                                                                                                                                                                              taataaAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAgg                                                                                               <  1:669571/158‑1 (MQ=255)                                                                                                                                                                                                                              taataaAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAAcg                         >  1:174603/1‑228 (MQ=255)                                                                                                                                                                                                                              taataaAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAgg  >  1:539705/1‑251 (MQ=255)                                                                                                                                                                                                                              taataaAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAgg  >  1:41383/1‑251 (MQ=255)                                                                                                                                                                                                                              taataaAGGCGGCGATGATGATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCGCGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACCGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAgg  >  1:363312/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         CGTGGTTTTGGTGTCCATTTCCAAGAAACGGCTTATTAACCAGAAACCGCTGTCGTTCACATGGGAGAATACGGTGGCGCCGGAGGCAATGGCAATCACGATAAAACATAAATCCAATTGGCTGAGATCGGTTGCGGCGGCAACGGTCGGGGCGATTAATGCGGCTGCAGTAGTCAGGGCTACCGTTGCCGAACCTTGTGCCACACGCAATGCCATAGAAATAATAAAGGCGGCGATGATAATCGGCATACCGGTATTTGACA‑ACATGGCGGATAACTCATCGCCGATGCCGCTGGCACGCAATACGCCACCGAACATACCGCCCGCACCGGTCACTAAAATAATGGAGCAAATCGGTCCCAGCGCACTGTTACAAAGGCTTTCGATTTGTTCATAACTGCGTTCTCCGCGCAATAAGGCAATGGCAATTAATAATGTGATTAATAACGCGATCGGGGTTTTGCCGATGAGG  >  1/1861954‑1862425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑