Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,860,029 G→A V557V (GTC→GTT)  unknown ← ATP‑dependent DNA helicase UvrD/PcrA (EC 3.6.4.12)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,860,0290GA100.0% 208.9 / NA 72V557V (GTC→GTT) unknownATP‑dependent DNA helicase UvrD/PcrA (EC 3.6.4.12)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/39);  total (33/39)

CGGTGATAAAACGGGAAGGCAAATGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCGACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTC  >  1/1859788‑1860274
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     
cGGTGATAAAACGGGAAGGCAAATGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGcc                                                                                                                                                                                                                                              <  1:95321/251‑1 (MQ=255)
     aTAAAACGGGAAGGCAAATGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGAtg                                                                                                                                                                                                                                         <  1:782748/251‑1 (MQ=255)
      tAAAACGGGAAGGCAAATGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGAtgt                                                                                                                                                                                                                                        <  1:849613/251‑1 (MQ=255)
      tAAAACGGGAAGGCAAATGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGAtgt                                                                                                                                                                                                                                        <  1:170201/251‑1 (MQ=255)
        aaaCGGGAAGGCAAATGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGAtg                                                                                                                                                                                                                                         <  1:693522/248‑1 (MQ=255)
           cGGGAAGGCAAATGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGgcg                                                                                                                                                                                                                                    >  1:484984/1‑250 (MQ=255)
                    aaaTGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGctc                                                                                                                                                                                                                          <  1:92916/251‑1 (MQ=255)
                                     gCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATg                                                                                                                                                                                                         >  1:172883/1‑251 (MQ=255)
                                     gCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGGAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATg                                                                                                                                                                                                         >  1:259658/1‑251 (MQ=255)
                                      ccATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGa                                                                                                                                                                                                        <  1:354976/251‑1 (MQ=255)
                                      ccATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGa                                                                                                                                                                                                        <  1:607150/251‑1 (MQ=255)
                                      ccATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGa                                                                                                                                                                                                        <  1:610093/251‑1 (MQ=255)
                                        atatAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTcc                                                                                                                                                                                                             >  1:219619/1‑244 (MQ=255)
                                        atatAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAg                                                                                                                                                                                                      <  1:602238/251‑1 (MQ=255)
                                        atatAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAg                                                                                                                                                                                                      <  1:620010/251‑1 (MQ=255)
                                         tataAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCACGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAg                                                                                                                                                                                                      >  1:510239/1‑250 (MQ=255)
                                          ataAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCg                                                                                                                                                                                                    <  1:557758/251‑1 (MQ=255)
                                                         ccGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGc                                                                                                                                                                                      >  1:810823/1‑250 (MQ=255)
                                                             aTAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCa                                                                                                                                                                                 <  1:111856/251‑1 (MQ=255)
                                                                  aaaTGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATc                                                                                                                                                                             <  1:614844/250‑1 (MQ=255)
                                                                    aTGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGt                                                                                                                                                                          >  1:493865/1‑251 (MQ=255)
                                                                       gTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCa                                                                                                                                                                        <  1:717174/250‑1 (MQ=255)
                                                                          aGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCatat                                                                                                                                                                     >  1:134020/1‑250 (MQ=255)
                                                                           gTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGAtgt                                                                                                                                                                                                                                        <  1:306649/182‑1 (MQ=255)
                                                                            tttCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCtt                                                                                                                                                                  >  1:404616/1‑251 (MQ=255)
                                                                            tttCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATAATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATc                                                                                                                                                                    <  1:196055/249‑1 (MQ=255)
                                                                                            gTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGtt                                                                                                                                                   <  1:95355/250‑1 (MQ=255)
                                                                                            gTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGtt                                                                                                                                                   <  1:880498/250‑1 (MQ=255)
                                                                                               aTCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGAtg                                                                                                                                                                                                                                         >  1:328285/1‑161 (MQ=255)
                                                                                                     aCGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAAtt                                                                                                                                          >  1:20983/1‑250 (MQ=255)
                                                                                                     aCGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTc                                                                                                                                         <  1:894966/251‑1 (MQ=255)
                                                                                                        tATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACg                                                                                                                                      >  1:532592/1‑251 (MQ=255)
                                                                                                                      ctctTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGt                                                                                                                                                                          >  1:103931/1‑201 (MQ=255)
                                                                                                                      ctctTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACAcc                                                                                                                         <  1:852175/250‑1 (MQ=255)
                                                                                                                      ctctTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCa                                                                                                                        >  1:227467/1‑251 (MQ=255)
                                                                                                                      ctctTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATAAAGCGCGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCa                                                                                                                        >  1:434712/1‑251 (MQ=255)
                                                                                                                          tcttcCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATg                                                                                                                                                                                                         >  1:38144/1‑166 (MQ=255)
                                                                                                                                  aCGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGt                                                                                                            <  1:571353/251‑1 (MQ=255)
                                                                                                                                             cttcAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAGATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACg                                                                                                  >  1:232659/1‑250 (MQ=255)
                                                                                                                                                      aaCGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCGTCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTcccc                                                                                         <  1:194663/250‑1 (MQ=255)
                                                                                                                                                       aCGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCtt                                                                                       <  1:344721/251‑1 (MQ=255)
                                                                                                                                                               tCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGccc                                                                               <  1:577202/251‑1 (MQ=255)
                                                                                                                                                               tCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGccc                                                                               <  1:753392/251‑1 (MQ=255)
                                                                                                                                                                cGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCaa                                                                                                      >  1:396035/1‑227 (MQ=255)
                                                                                                                                                                         tCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATc                                                                                                                                                                             >  1:703898/1‑147 (MQ=255)
                                                                                                                                                                         tCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAAttc                                                                                                                    >  1:388727/1‑204 (MQ=255)
                                                                                                                                                                         tCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGtt                                                                     <  1:848241/251‑1 (MQ=255)
                                                                                                                                                                             tcttcCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATc                                                                                                                                                                             >  1:613666/1‑143 (MQ=255)
                                                                                                                                                                               ttcCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCTGACACCAAttc                                                                                                                    <  1:235705/198‑1 (MQ=255)
                                                                                                                                                                                   ccGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTg                                                           <  1:173154/250‑1 (MQ=255)
                                                                                                                                                                                       cAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATAc                                                       <  1:853839/251‑1 (MQ=255)
                                                                                                                                                                                        aaTCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGtt                                                                                                                                                   >  1:703286/1‑158 (MQ=255)
                                                                                                                                                                                            aTGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAAccc                                                  <  1:792026/251‑1 (MQ=255)
                                                                                                                                                                                                 aaCACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAAt                                             <  1:769372/251‑1 (MQ=255)
                                                                                                                                                                                                          aaaCTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATca                                    <  1:730571/251‑1 (MQ=255)
                                                                                                                                                                                                             cTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTcgcc                                                                                                                                                                                                                    <  1:198601/72‑1 (MQ=255)
                                                                                                                                                                                                             cTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATc                                                                                                                                                                             <  1:428690/111‑1 (MQ=255)
                                                                                                                                                                                                             cTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACaa                                 >  1:702108/1‑251 (MQ=255)
                                                                                                                                                                                                             cTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACaa                                 >  1:21712/1‑251 (MQ=255)
                                                                                                                                                                                                                 aaGCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATc                             <  1:314693/251‑1 (MQ=255)
                                                                                                                                                                                                                   gCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATAc                                                       >  1:632251/1‑223 (MQ=255)
                                                                                                                                                                                                                   gCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAAccc                                                  >  1:622001/1‑228 (MQ=255)
                                                                                                                                                                                                                   gCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACaaaa                               <  1:121768/247‑1 (MQ=255)
                                                                                                                                                                                                                   gCCTTTTACCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCg                           >  1:539257/1‑251 (MQ=255)
                                                                                                                                                                                                                          aCCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTa                                        >  1:577582/1‑231 (MQ=255)
                                                                                                                                                                                                                          aCCGAATGCAGCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTgcg                     >  1:660590/1‑250 (MQ=255)
                                                                                                                                                                                                                          aCCGAATGCAGCATCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGCCAGATCCGACATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTgcg                     >  1:78286/1‑250 (MQ=255)
                                                                                                                                                                                                                                    gCGTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATAc                                                       <  1:699673/206‑1 (MQ=255)
                                                                                                                                                                                                                                      gTCATCATTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTc                                                                                                                                                           >  1:502665/1‑104 (MQ=255)
                                                                                                                                                                                                                                            atTTCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATttcttc  <  1:281614/251‑1 (MQ=255)
                                                                                                                                                                                                                                             tttCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATttcttc  >  1:792460/1‑250 (MQ=255)
                                                                                                                                                                                                                                             tttCAACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATttcttc  >  1:235853/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     
CGGTGATAAAACGGGAAGGCAAATGACGTTCTTCCTTGCCATATAAACGGCGGCTTTCCGCATAAGAAATGGTGAGTTTCTGCTTGGCGCGGGTAATCCCGACGTATGCCAAACGGCGCTCTTCTTCCAGACGCCCCGCTTCTTCAAAAGAACGGAAACTCGGGAATAATCCTTCTTCCACGCCAATCATGAAAACACGCGGAAACTCCAAGCCTTTTACCGAATGCAGCGTCATCATTTCGACGCAAGCCTGATGTGGCGCAGCCTGCTCCTCGCCCGCCTCCAATGAAGCGTGAGTTAAAAATGCCGTCAGATCCGTCATATCTTCCGCGTCTTCAGGTTTGATAAATTCACGGGCGGCAGACACCAATTCTTCCAGGTTTTCAATACGCACCTCCCCTTTTTCGCCCTTTTCCTGTTTGTACATTTGATACAACCCGGAATGCTTAATCACAAAATCCGTTTGCGCAAACAACGGCATTTCTTC  >  1/1859788‑1860274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑