Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,849,870	C→T	S269N (AGC→AAC)	unknown ←	Membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,849,870	0	C	T	100.0%	177.2 / NA	62	S269N (AGC→AAC)	unknown	Membrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/32);  total (30/32)

CTGTAATTATTAGAGACCAAAAATGCACGCCCTAATTCACGATCCGGCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGCTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGATCGCGCTGTAGCATTTTCATGGCGGCGATGAATTCTTTGCTGAATAAGGC  >  1/1849630‑1850102                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                          cTGTAATTATTAGAGACCAAAAATGCACGCCCTAATTCACGATCCGGCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGa                                                                                                                                                                                                                                  >  1:624563/1‑250 (MQ=255)                aCCAAAAATGCACGCCCTAATTCACGATCCGGCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAaa‑taa                                                                                                                                                                                                                   <  1:108842/249‑1 (MQ=255)                aCCAAAAATGCACGCCCTAATTCACGATCCGGCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAAtt                                                                                                                                                                                                                 <  1:369414/251‑1 (MQ=255)                 ccAAAAATGCACGCCCTAATTCACGATCCGGCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTaaa                                                                                                                                                                                                                       <  1:760306/245‑1 (MQ=255)                        tGCACGCCCTAATTCACGATCCGGCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATgg                                                                                                                                                                                                         <  1:238437/251‑1 (MQ=255)                           aCGCCCTAATTCACGATCCGGCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAgg                                                                                                                                                                                                      <  1:694308/251‑1 (MQ=255)                              cccTAATTCACGATCCGGCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGa                                                                                                                                                                                                   >  1:363518/1‑251 (MQ=255)                                         gATCCGGCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAaa‑taa                                                                                                                                                                                                                   >  1:515220/1‑224 (MQ=255)                                          aTCCGGCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATaa                                                                                                                                                                                        <  1:10980/250‑1 (MQ=255)                                             cGGCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATTATAATGAt                                                                                                                                                                                    <  1:861647/251‑1 (MQ=255)                                               gCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATc                                                                                                                                                                                   <  1:858621/250‑1 (MQ=255)                                               gCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATc                                                                                                                                                                                   <  1:737720/250‑1 (MQ=255)                                               gCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATc                                                                                                                                                                                   <  1:450726/250‑1 (MQ=255)                                               gCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATc                                                                                                                                                                                   <  1:427947/250‑1 (MQ=255)                                               gCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCg                                                                                                                                                                                  >  1:456666/1‑251 (MQ=255)                                                        gCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGata                                                                                                                                                                         >  1:437528/1‑251 (MQ=255)                                                         ccATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGata                                                                                                                                                                         <  1:42842/250‑1 (MQ=255)                                                               gTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTcc                                                                                                                                                                              <  1:655097/239‑1 (MQ=255)                                                                      gcgcCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCcat                                                                                                                                                            >  1:109878/1‑250 (MQ=255)                                                                          cGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCAttg                                                                                                                                                       <  1:347861/251‑1 (MQ=255)                                                                           ggAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGata                                                                                                                                                                         >  1:613002/1‑232 (MQ=255)                                                                              aaGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGc                                                                                                                                                   <  1:16738/251‑1 (MQ=255)                                                                                       cAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAaa‑taa                                                                                                                                                                                                                   >  1:282516/1‑178 (MQ=255)                                                                                         gTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTgcggcg                                                                                                                                        >  1:733321/1‑251 (MQ=255)                                                                                          tttttGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGt                                                                                                                                       <  1:566130/251‑1 (MQ=255)                                                                                          tttttGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGt                                                                                                                                       <  1:660535/251‑1 (MQ=255)                                                                                                   tgtTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGt                                                                                                                              <  1:768877/251‑1 (MQ=255)                                                                                                                  gTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAGCATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGa                                                                                                               >  1:452949/1‑251 (MQ=255)                                                                                                                    aTTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGata                                                                                                                                                                         >  1:726094/1‑191 (MQ=255)                                                                                                                    aTTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAAt                                                                                                             >  1:367248/1‑251 (MQ=255)                                                                                                                    aTTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAAt                                                                                                             <  1:423962/251‑1 (MQ=255)                                                                                                                    aTTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAAt                                                                                                             >  1:172169/1‑251 (MQ=255)                                                                                                                           ggTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGt                                                                                                        <  1:656583/249‑1 (MQ=255)                                                                                                                                      tatTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAAtt                                                                                                                                                                                                                 <  1:314908/133‑1 (MQ=255)                                                                                                                                      tatTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTg                                                                                                                                  >  1:304249/1‑212 (MQ=255)                                                                                                                                      tatTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTcc                                                                                                  >  1:725751/1‑244 (MQ=255)                                                                                                                                                    cTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAAc                                                                              >  1:261902/1‑250 (MQ=255)                                                                                                                                                        aaGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGc                                                                         <  1:700827/251‑1 (MQ=255)                                                                                                                                                                            tctGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAATTAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAatat                                                      <  1:548891/251‑1 (MQ=255)                                                                                                                                                                              tgtgCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGAt                                                   <  1:521562/251‑1 (MQ=255)                                                                                                                                                                              tgtgCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGAt                                                   <  1:860383/251‑1 (MQ=255)                                                                                                                                                                               gtgCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGAt                                                   >  1:898021/1‑250 (MQ=255)                                                                                                                                                                               gtgCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGAt                                                   >  1:576859/1‑250 (MQ=255)                                                                                                                                                                               gtgCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGAt                                                   >  1:133434/1‑250 (MQ=255)                                                                                                                                                                                                gTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGAt                                                   >  1:377019/1‑233 (MQ=255)                                                                                                                                                                                                gTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGATCGCGCTGTAGCATTTTCa                                 >  1:675656/1‑251 (MQ=255)                                                                                                                                                                                                gTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGATCGCGCTGTAGCATTTTCa                                 >  1:730174/1‑251 (MQ=255)                                                                                                                                                                                                 tCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGccc                                                                                    >  1:18525/1‑199 (MQ=255)                                                                                                                                                                                                            ggTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGcc                                                                                                                                                              <  1:226301/114‑1 (MQ=255)                                                                                                                                                                                                                aGTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGATCGCGCTGTAGCATTTTCATGGCGGCGATGAAtt                  >  1:546137/1‑250 (MQ=255)                                                                                                                                                                                                                 gTGCAACTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGt                                                             <  1:748058/206‑1 (MQ=255)                                                                                                                                                                                                                     aaCTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGATCGCGCTGTAGCATTTTCATGGCGGCGATGAATTCTTTGc            <  1:160777/251‑1 (MQ=255)                                                                                                                                                                                                                       cTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGt                                                                                                        >  1:279721/1‑157 (MQ=255)                                                                                                                                                                                                                       cTTCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGATCGCGCTGTAGCATTTTCATGGCGGCGATGAATTCTTTg             >  1:114521/1‑248 (MQ=255)                                                                                                                                                                                                                         tCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATgg                                                                                             >  1:105607/1‑166 (MQ=255)                                                                                                                                                                                                                         tCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGgcg                                                                                           <  1:254864/168‑1 (MQ=255)                                                                                                                                                                                                                         tCTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATg                                                     <  1:487653/206‑1 (MQ=255)                                                                                                                                                                                                                          cTACACCCCATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGATCGCGCTGTAGCATTTTCATGGCGGCGATGAATTCTTTGCTGAAt       >  1:527416/1‑251 (MQ=255)                                                                                                                                                                                                                               ccccATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGAt                                                   <  1:754275/202‑1 (MQ=255)                                                                                                                                                                                                                               ccccATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGAt                                                   >  1:738141/1‑202 (MQ=255)                                                                                                                                                                                                                               ccccATGGCAGGTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGATCGCGCTGTAGCATTTTCATGGCGGCGATGAATTCTTTGCTGAATAAGGc  <  1:548364/251‑1 (MQ=255)                                                                                                                                                                                                                                          gTTTTCGTTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAgg                                                                                                                               >  1:217939/1‑115 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                          CTGTAATTATTAGAGACCAAAAATGCACGCCCTAATTCACGATCCGGCCTTACCAGCCATAAGTCTTGTGCGCCGGAAAGTGCGGTCAGTTTTTGTTGTGTTTGTGGAGTTTGGTATTTTAGGGTGACACCCATATTTTGCCAGTCGCTTAAAGAACGTTTTTTATTTTCCTCTGTGCCAGCAAGCGCCATGTCTAACGGTTGGGTTAGTGCAACTTCTACACCCCATGGCAGGTTTTCGCTCCAGCCGACGGTGTGTAAA‑TAATTAGCGATGGAGGCGAAAACATCATAATGATCGGTCCAGATATTTTTCTCGCCATCATTGTTGCCATCTGCGGCGTAGCTGAGGTAAGAGCTTGGCATGAATTGGGTCTGTCCCATGGCGCCTGCCCATGAACCCAGCATTTTATAGCGTTGAATATGATCGCGCTGTAGCATTTTCATGGCGGCGATGAATTCTTTGCTGAATAAGGC  >  1/1849630‑1850102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑