Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,840,594	A→G	L335S (TTA→TCA)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,840,594	0	A	G	100.0%	21.9 / NA	9	L335S (TTA→TCA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/5);  total (4/5)

AAATAAAAGAAGAAAAAATAATAAGCATGATCGGAATAAAATAGCGTCCTTGAACTCCTACTATTTGTGTTGTGCCAATTTCATTATAAGTAATCAAGAGAATAAAATGGGTAAATAAAAATACAACTCCTACTAAAAGAACACTTACTATAACATATTTTAGTTTATACAAGTTGTGAATGAATAGAAAAATATAAGCCAATAATAAAGTAGAACCGAAAAATAGATACTCATTTATAGTAAATTTCGTATCAACCCATCCAAGAACGCCTAAAAACATTCTCAGCAAAGATTTTAAATAAGTAAGATTTAAAGTATTTTTAAAAATTTCAAATAAGTCATCCATATGAAAAATATAATAAGAGAAATTATGTAATGCGCCTTCTTTGAAGTGAGATTGAGCCTCAGTTAATTTCATAGCCAAAAC  >  1/1840388‑1840814                                                                                                                                                                                                               |                                                                                                                                                                                                                             aaataaaaGAAGAAAAAATAATAAGCATGATCGGAATAAAATAGCGTCCTTGAACTCCTACTATTTGTGTTGTGCCAATTTCATTATAAGTAATCAAGAGAATAAAATGGGTAAATAAAAATACAACTCCTACTAAAAGAACACTTACTATAACATATTTTAGTTTATACAAGTTGTGAATGAATAGAAAAATATAAGCCAATAATGAAGTAg                                                                                                                                                                                                                        <  1:144668/213‑1 (MQ=255)                                                         ccTACTATTTGTGTTGTGCCAATTTCATTATAAGTAATCAAGAGAATAAAATGGGTAAATAAAAATACAACTCCTACTAAAAGAACACTTACTATAACATATTTTAGTTTATACAAGTTGTGAATGAATAGAAAAATATAAGCCAATAATGAAGTAGAACCGAAAAATAGATACTCATTTATAGTAAATTTCGTATCAACCCATCCAAGAACGCCTAAAAACATtctc                                                                                                                                                 <  1:447578/228‑1 (MQ=255)                                                               aTTTGTGTTGTGCCAATTTCATTATAAGTAATCAAGAGAATAAAATGGGTAAATAAAAATACAACTCCTACTAAAAGAACACTTACTATAACATATTTTAGTTTATACAAGTTGTGAATGAATAGAAAAATATAAGCCAATAATGAAGTAGAACCGAAAAATAGATACTCATTTATAGTAAATTTCGTATCAACCCATCCAAGAACGCCTAAAAACATTCTCAGCAAAGATTTTAAATAAGTAAGATTTaa                                                                                                                    >  1:65020/1‑251 (MQ=255)                                                                               tttCATTATAAGTAATCAAGAGAATAAAATGGGTAAATAAAAATACAACTCCTACTAAAAGAACACTTACTATAACATATTTTAGTTTATACAAGTTGTGAATGAATAGAAAAATATAAGCCAATAATGAAGTAGAAc                                                                                                                                                                                                                     <  1:549688/138‑1 (MQ=255)                                                                                                    gaATAAAATGGGTAAATAAAAATACAACTCCTACTAAAAGAACACTTACTATAACATATTTTAGTTTATACAAGTTGTGAATGAATAGAAAAATATAAGCCAATAATGAAGTAGAACCGAAAAATAGATACTCATTTATAGTAAATTTCGTATCAACCCATCCAAGAACGCCTAAAAACATTCTCAGCAAAGa                                                                                                                                         >  1:711920/1‑193 (MQ=255)                                                                                                                                                                  aGTTTATACAAGTTGTGAATGAATAGAAAAATATAAGCCAATAATGAAGTAGAACCGAAAAATAGATACTCATTTATAGTAAATTTCGTATCAACCCATCCAAGAACGCCTAAAAACATTCTCAGCAAAGATTTTAAATAAGTAAGATTTAAAGTATTTTTAAAAATTTCAAATAAGTCATCCATATGAAAAATATAATAAGAGAAATTATGt                                                       <  1:190791/213‑1 (MQ=255)                                                                                                                                                                                gtgAATGAATAGAAAAATATAAGCCAATAATGAAGTAGAACCGAAAAATAGATACTCATTTATAGTAAATTTCGTATCAACCCATCCAAGAACGCCTAAAAACATTCTCAGCAAAGATTTTAAATAAGTAAGATTTAAAGTATTTTTAAAAATTTCAAATAAGTCATCCATATGAAAAATATAATAAGAGAAATTATGTAATGCGCCTTCTTTGAAGTGAGATTGAGCCTCAGTTAATTTCATAGCCaaaa   >  1:68612/1‑251 (MQ=255)                                                                                                                                                                                           gAAAAATATAAGCCAATAATGAAGTAGAACCGAAAAATAGATACTCATTTATAGTAAATTTCGTATCAACCCATCCAAGAACGCCTAAAAACATTCTCAGCAAAGATTTTaaa                                                                                                                                  <  1:407039/113‑1 (MQ=255)                                                                                                                                                                                                atatAAGCCAATAATGAAGTAGAACCGAAAAATAGATACTCATTTATAGTAAATTTCGTATCAACCCATCCAAGAACGCCTAAAAACATTCTCAGCAAAGATTTTAAATAAGTAAGATTTAAAGTATTTTTAAAAATTTCAAATAAGTCATCCATATGAAAAATATAATAAGAGAAATTATGTAATGCGCCTTCTTTGAAGTGAGATTGAGCCTCAGTTAATTTCATAGCCAAAAc  >  1:837628/1‑236 (MQ=255)                                                                                                                                                                                                           taatgaAGTAGAACCGAAAAATAGATACTCATTTATAGTAAATTTCGTATCAACCCATCCAAGAACGCCTAAAAAc                                                                                                                                                       <  1:595127/76‑1 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                             AAATAAAAGAAGAAAAAATAATAAGCATGATCGGAATAAAATAGCGTCCTTGAACTCCTACTATTTGTGTTGTGCCAATTTCATTATAAGTAATCAAGAGAATAAAATGGGTAAATAAAAATACAACTCCTACTAAAAGAACACTTACTATAACATATTTTAGTTTATACAAGTTGTGAATGAATAGAAAAATATAAGCCAATAATAAAGTAGAACCGAAAAATAGATACTCATTTATAGTAAATTTCGTATCAACCCATCCAAGAACGCCTAAAAACATTCTCAGCAAAGATTTTAAATAAGTAAGATTTAAAGTATTTTTAAAAATTTCAAATAAGTCATCCATATGAAAAATATAATAAGAGAAATTATGTAATGCGCCTTCTTTGAAGTGAGATTGAGCCTCAGTTAATTTCATAGCCAAAAC  >  1/1840388‑1840814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑