| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,829,582 | G→T | A44A (GCC→GCA) | unknown ← | N‑acetyl‑D‑glucosamine kinase (EC 2.7.1.59) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,829,582 | 0 | G | T | 98.4% | 178.9 / ‑4.2 | 63 | A44A (GCC→GCA) | unknown | N‑acetyl‑D‑glucosamine kinase (EC 2.7.1.59) |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (39/23); total (40/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01 | |||||||||||
AATACGGAAGGATATTCGGCGTTTTCCGCATCCCAGGCTTCCGACAAGGCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCGGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGTTTCTCCTGTTAAAATACCTAAAAAAACGACCGCACTTTTGGCAAACTTATTCCGCTTTTTGGAAATACCGCTTTTTGGCTAAAAATGCGAACATCACCCAATGC > 1/1829337‑1829821 | aaTACGGAAGGATATTCGGCGTTTTCCGCATCCCAGGCTTCCGACAAGGCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAAt < 1:182662/251‑1 (MQ=255) tACGGAAGGATATTCGGCGTTTTCCGCATCCCAGGCTTCCGACAAGGCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAg < 1:675403/251‑1 (MQ=255) tACGGAAGGATATTCGGCGTTTTCCGCATCCCAGGCTTCCGACAAGGCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAg < 1:127354/251‑1 (MQ=255) tACGGAAGGATATTCGGCGTTTTCCGCATCCCAGGCTTCCGACAAGGCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAg < 1:829684/251‑1 (MQ=255) gATATTCGGCGTTTTCCGCATCCCAGGCTTCCGACAAGGCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAg > 1:666362/1‑243 (MQ=255) atatTCGGCGTTTTCCGCATCCCAGGCTTCCGACAAGGCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAAt > 1:410456/1‑240 (MQ=255) gCGTTTTCCGCATCCCAGGCTTCCGACAAGGCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATACTAGGGATTTCCGCAATGCAGGTGGCTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAg > 1:72824/1‑235 (MQ=255) gCGTTTTCCGCATCCCAGGCTTCCGACAAGGCAAAGCAGTTAGAATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGGGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCtt < 1:827427/250‑1 (MQ=255) tCCCAGGCTTCCGACAAGGCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGtttt > 1:57159/1‑251 (MQ=255) tCCCAGGCTTCCGACAAGGCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGtttt > 1:736846/1‑251 (MQ=255) gCTTCCGACAAGGCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGt > 1:424524/1‑251 (MQ=255) cGACAAGGCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCa > 1:266888/1‑251 (MQ=255) aGGCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTc > 1:708442/1‑212 (MQ=255) aGGCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGt > 1:109353/1‑251 (MQ=255) ggCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAg > 1:790189/1‑206 (MQ=255) gCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGtt > 1:101275/1‑250 (MQ=255) gCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGtttt > 1:777296/1‑228 (MQ=255) cATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCtt < 1:841904/250‑1 (MQ=255) cTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAac < 1:876418/251‑1 (MQ=255) ttCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTGGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCGGCAATAGTGTTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCa > 1:141348/1‑251 (MQ=255) ttCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCg > 1:509598/1‑200 (MQ=255) ttCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCa > 1:256279/1‑251 (MQ=255) ttCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCa > 1:458584/1‑251 (MQ=255) aaaaTAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTc < 1:612191/251‑1 (MQ=255) cGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAAt > 1:876215/1‑154 (MQ=255) cGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAAttt < 1:86360/249‑1 (MQ=255) cacaAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAat > 1:654190/1‑251 (MQ=255) caAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAAt > 1:212345/1‑141 (MQ=255) aaaTGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATc > 1:355189/1‑250 (MQ=255) aTGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCTATATCCaa > 1:645764/1‑251 (MQ=255) gTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAAt > 1:186071/1‑129 (MQ=255) gTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAg > 1:213788/1‑151 (MQ=255) gTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCCCCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGtata > 1:444387/1‑251 (MQ=255) cccGCCAGGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACAt < 1:568043/250‑1 (MQ=255) ccgcgGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGACCCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGttt < 1:339415/250‑1 (MQ=255) ccgcgGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGttt < 1:520088/250‑1 (MQ=255) aCGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGACCCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGttt < 1:775568/251‑1 (MQ=255) aCGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGttt < 1:139493/251‑1 (MQ=255) cgGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGTTtctc < 1:241372/251‑1 (MQ=255) gATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACAt > 1:516967/1‑240 (MQ=255) gATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGTTtctc > 1:622626/1‑249 (MQ=255) gATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGTTtctc > 1:717626/1‑249 (MQ=255) gATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGTTTCTcc < 1:529890/250‑1 (MQ=255) tatTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATcc > 1:870970/1‑224 (MQ=255) tatTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGTTtctc > 1:326971/1‑247 (MQ=255) ttAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTc > 1:200142/1‑117 (MQ=255) ttAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACAt < 1:818002/236‑1 (MQ=255) ttAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATc > 1:667259/1‑237 (MQ=255) aGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAAt > 1:249930/1‑223 (MQ=255) aTTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGTTTCTCCTGTTAAAATAc < 1:877015/251‑1 (MQ=255) aTTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGTTTCTCCTGTTAAAATAc < 1:218474/251‑1 (MQ=255) aTTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGTTTCTCCTGTTAAAATAc < 1:734269/251‑1 (MQ=255) aTGCCGGTGGTTTGATTCACAAAGCCGGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACAt < 1:561197/221‑1 (MQ=255) tGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGTTtctc > 1:644029/1‑229 (MQ=255) gCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATc > 1:714082/1‑199 (MQ=255) gCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGTTTCTCCTGTTAAAATACCTAAAAAAACGACCGCACTTTTGGCAAACTTATTCCGCttt > 1:268166/1‑251 (MQ=255) aaGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGTTTCTCCTGTTAAAATACCTAAAAAAACGACCGCACTTTTGGCAAACTTATTCCGCTTTTTgg < 1:482232/251‑1 (MQ=255) gACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGTTTCTCCTGTTAAAATACCTAAAAAAACGACCGCACTTTTGGCAAACTTATTCCGCTTTTTGGAAATa > 1:456587/1‑251 (MQ=255) gAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTgg > 1:477223/1‑148 (MQ=255) gAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATcc > 1:363385/1‑162 (MQ=255) gAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATcc > 1:84357/1‑162 (MQ=255) ttCACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGTTTCTCCTGTTAAAATACCTAAAAAAACGACCGCACTTTTGGCAAACTTATTCCGCTTTTTGGAAATACCGCTTTTTGGCTAAAAATGCGAACATCACCCAATGc < 1:169991/251‑1 (MQ=255) cACCAAATCTGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACAt > 1:709023/1‑141 (MQ=255) | AATACGGAAGGATATTCGGCGTTTTCCGCATCCCAGGCTTCCGACAAGGCAAAGCAGTTAGCATCGTTTTCGGCGCGTACTTCGCGTTCTAAAATAGCGGATAAGTCGCACAAAATGGGTTTGTTATCCGCCACGCGGATATTAGTGATTTCCGCAATGCCGGTGGTTTGATTCACAAAGCCCGGAATGCCTAAGCCGACGGAACCTTTACCGCCGAATTTTTCGTCGGCTTTTTTCACCAAATCGGCAATAGTATTCAGCCAATCTTCATAGCTGGTTTTCGGCGTTTCCACCCGTTCGCGATATTGCTTCTCCAACTGCGAGTTAAACACCGCCAACTCAATTTTGGTTCCGCCAATATCCAATCCGTATAACATCTGTTTCTCCTGTTAAAATACCTAAAAAAACGACCGCACTTTTGGCAAACTTATTCCGCTTTTTGGAAATACCGCTTTTTGGCTAAAAATGCGAACATCACCCAATGC > 1/1829337‑1829821 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |