Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,829,010	A→G	V235A (GTG→GCG)	unknown ←	N‑acetyl‑D‑glucosamine kinase (EC 2.7.1.59)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,829,010	0	A	G	100.0%	201.4 / NA	71	V235A (GTG→GCG)	unknown	N‑acetyl‑D‑glucosamine kinase (EC 2.7.1.59)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/33);  total (38/33)

CCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCACGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCGAGTAATTTCAACGCATGGTAGTTCAATTGCAAATGCCCTAATTC  >  1/1828778‑1829254                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     ccTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACaaa                                                                                                                                                                                                                                      <  1:525643/249‑1 (MQ=255)          ttGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACaa                                                                                                                                                                                                                           <  1:867922/251‑1 (MQ=255)                    cATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCg                                                                                                                                                                                                                  >  1:45370/1‑250 (MQ=255)                            ggATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTAt                                                                                                                                                                                                         >  1:460519/1‑251 (MQ=255)                              aTAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCg                                                                                                                                                                                                       <  1:834085/251‑1 (MQ=255)                                          ccgcGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGaa                                                                                                                                                                                            <  1:658108/250‑1 (MQ=255)                                               gcgcCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTg                                                                                                                                                                                      >  1:851696/1‑251 (MQ=255)                                                       cacgcCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAAttt                                                                                                                                                                              <  1:614966/251‑1 (MQ=255)                                                                     cGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAg                                                                                                                                                                >  1:95645/1‑251 (MQ=255)                                                                                   tttttAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCGCTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGcc                                                                                                                                                        <  1:786499/245‑1 (MQ=255)                                                                                           cACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGGAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAACGCGGGTCAAAGGCGGGAATGATATTGCCGATGGAAATCGCGGCAAGCCCCACAAAAACGCCGACAAAATCCACCGCCCCTTTATCGCTCGCGTAGAAAAGTTGAATAATTTCTTTGGCCGATTAGGATTCGCCTTTTAAATCGCGGt                                                                                                                                          >  1:742911/1‑251 (MQ=255)                                                                                                gCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATaaa                                                                                                                                     >  1:769952/1‑250 (MQ=255)                                                                                                        gcgcTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACAt                                                                                                                                   >  1:807714/1‑245 (MQ=255)                                                                                                           cTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAAc                                                                                                                           <  1:334905/250‑1 (MQ=255)                                                                                                                cATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGa                                                                                                                                                              >  1:690646/1‑210 (MQ=255)                                                                                                                cATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAAc                                                                                                                                     >  1:375141/1‑235 (MQ=255)                                                                                                                cATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGa                                                                                                                      >  1:349153/1‑250 (MQ=255)                                                                                                                cATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGa                                                                                                                      >  1:473112/1‑250 (MQ=255)                                                                                                                           gcggTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTa                                                                                                                                                   >  1:505283/1‑210 (MQ=255)                                                                                                                           gcggTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAg                                                                                                          >  1:861776/1‑251 (MQ=255)                                                                                                                            cggTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAgg                                                                                                         <  1:616008/251‑1 (MQ=255)                                                                                                                            cggTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAgg                                                                                                         <  1:709260/251‑1 (MQ=255)                                                                                                                            cggTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAgg                                                                                                         <  1:62504/251‑1 (MQ=255)                                                                                                                            cggTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAg                                                                                                          <  1:606210/250‑1 (MQ=255)                                                                                                                              gTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGgtg                                                                                                       >  1:874367/1‑251 (MQ=255)                                                                                                                                    cTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCaaa                                                                                                 >  1:812659/1‑251 (MQ=255)                                                                                                                                     tttCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAAc                                                                                                <  1:434579/251‑1 (MQ=255)                                                                                                                                     tttCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAAc                                                                                                <  1:153327/251‑1 (MQ=255)                                                                                                                                          gTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAgg                                                                                                         <  1:197444/237‑1 (MQ=255)                                                                                                                                          gTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAgg                                                                                             >  1:206167/1‑249 (MQ=255)                                                                                                                                           tAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCt                                                                                           <  1:584278/250‑1 (MQ=255)                                                                                                                                               gCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTAtt                                                                                      >  1:639734/1‑251 (MQ=255)                                                                                                                                                cACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTAtt                                                                                      <  1:6787/250‑1 (MQ=255)                                                                                                                                                  ccGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGaat                                                                                                                                                                                   <  1:146677/155‑1 (MQ=255)                                                                                                                                                  ccGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGc                                                                                    >  1:598023/1‑250 (MQ=255)                                                                                                                                                    gTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAAt                                                                                                                                                >  1:24044/1‑188 (MQ=255)                                                                                                                                                    gTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAgg                                                                                                         <  1:687654/227‑1 (MQ=255)                                                                                                                                                    gTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAgg                                                                                                         <  1:399113/227‑1 (MQ=255)                                                                                                                                                    gTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCg                                                                                  >  1:247140/1‑250 (MQ=255)                                                                                                                                                            aatCAAAATTGGACAATCCGCCGCCGAGCACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCCAGCATAGAAACGTTGAATAATTTCTTTGGCGGATAAAGCCTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAg                                                                         >  1:459418/1‑251 (MQ=255)                                                                                                                                                                gAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTAGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGt                                                                     >  1:254594/1‑251 (MQ=255)                                                                                                                                                                 aaaTTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTa                                                                    >  1:890627/1‑251 (MQ=255)                                                                                                                                                                 aaaTTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTa                                                                    >  1:662481/1‑251 (MQ=255)                                                                                                                                                                     tGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATc                                                                <  1:774995/251‑1 (MQ=255)                                                                                                                                                                           aTCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGc                                                           >  1:579454/1‑250 (MQ=255)                                                                                                                                                                           aTCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGc                                                           >  1:641147/1‑250 (MQ=255)                                                                                                                                                                                 cgccgAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTcc                                                    <  1:334174/251‑1 (MQ=255)                                                                                                                                                                                     gAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAg                                                 >  1:526445/1‑250 (MQ=255)                                                                                                                                                                                     gAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAg                                                 >  1:172965/1‑250 (MQ=255)                                                                                                                                                                                      aGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCGCCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAGCAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGc                                                <  1:134936/250‑1 (MQ=255)                                                                                                                                                                                      aGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCTGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGc                                                <  1:873357/250‑1 (MQ=255)                                                                                                                                                                                       gTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAGATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCg                                              >  1:371841/1‑251 (MQ=255)                                                                                                                                                                                       gTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCcacat                                                                                                                                                                                                                                       >  1:454377/1‑65 (MQ=255)                                                                                                                                                                                           gATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCGAGTa                                          >  1:619234/1‑251 (MQ=255)                                                                                                                                                                                           gATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCAAAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCGAGTa                                          >  1:286879/1‑251 (MQ=255)                                                                                                                                                                                            aTCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTAtt                                                                                      >  1:807507/1‑206 (MQ=255)                                                                                                                                                                                            aTCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCGAGTaa                                         >  1:296571/1‑251 (MQ=255)                                                                                                                                                                                                      ggTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCGAGTAATTTCAACGCa                               >  1:122247/1‑251 (MQ=255)                                                                                                                                                                                                       gTCAAAGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCGAGTAATTTCAACGCa                               <  1:210229/250‑1 (MQ=255)                                                                                                                                                                                                          aaaGGCGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCGAGTAATTTCAACGCATGGt                           <  1:712510/251‑1 (MQ=255)                                                                                                                                                                                                               cGGTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCGAGTAATTTCAACGCATGGTAGTTc                      <  1:543381/251‑1 (MQ=255)                                                                                                                                                                                                                 gTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAAc                                                                                                <  1:312176/175‑1 (MQ=255)                                                                                                                                                                                                                 gTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCGAGTAATTTCAACGCATGGt                           <  1:395357/244‑1 (MQ=255)                                                                                                                                                                                                                 gTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCGAGTAATTTCAACGCATGGTAGTTc                      <  1:733428/249‑1 (MQ=255)                                                                                                                                                                                                                 gTAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCGAGTAATTTCAACGCATGGTAGTTCaa                    >  1:550818/1‑251 (MQ=255)                                                                                                                                                                                                                  tAATGATATTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCGAGTAATTTCAACGCATGGt                           >  1:345558/1‑243 (MQ=255)                                                                                                                                                                                                                         atTGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCGAGTAATTTCAACGCATGGTAGTTCAATTGCAAAt            <  1:277783/251‑1 (MQ=255)                                                                                                                                                                                                                           tGCCGATGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCGAGTAATTTCAACGCATGGt                           <  1:849028/234‑1 (MQ=255)                                                                                                                                                                                                                                aTGGAAATCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAg                                                                                                                                                                >  1:378877/1‑96 (MQ=255)                                                                                                                                                                                                                                    aaaTCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCGAGTAATTTCAACGCATGGTAGTTCAATTGCAAATGCCCTAATTc  <  1:269826/250‑1 (MQ=255)                                                                                                                                                                                                                                     aaTCGCGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAAcc                                                                             <  1:489607/174‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     CCTTTTTTATTGCGTTAAACATTAACGGGATAAAAACAACGCCGCGGCGCCACGCACGCCGCCGGAGTCGCCATATTTGGCTTTTTTAATCACCGGCACTTTGGCGCTGCGCATTAAATGCGGCGGTAAAGCTTTCGGTAAGGCACCGTATAAATAATCGAAATTGGACAATCCGCCGCCGAGTACGATCAAATGCGGGTCAAAGGCGGTAATGATATTGCCGATGGAAATCACGGCAAGCTCCACAAAAACGCCGACAAAATCCACCGCACTTTTATCGCTGGCGTAGAAACGTTGAATAATTTCTTTGGCCGATAAGGATTCGCCTTTTAAATCGCGGTATAACATTTCAAAACCGCGACCGGATAAATAGGTGTCCAAACAGGCTTTATTGCCGCAACCGCAGTCGTAAATCGGTGCCTTGTCCCAGCCGAGTAATTTCAACGCATGGTAGTTCAATTGCAAATGCCCTAATTC  >  1/1828778‑1829254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑