Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,809,672	C→T	intergenic (+5/+68)	unknown → / ← unknown	hypothetical protein/F200

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,809,672	0	C	T	100.0%	46.0 / NA	18	intergenic (+5/+68)	unknown/unknown	hypothetical protein/F200
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/9);  total (9/9)

GCCGTTTTCAAGTGTAATTGTTTTTGGAAAACCGCAACTAGGTACCCCAATGATGATTGAATCCCCAACCCTAGCTATTGATTTACCATTAAAAGCTTTAGTATGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTACTTATTATAGTACTTTATAAATACAAAAATAAGTTCGCCTAAATTGAGGCGAACTTATTTATTACCGACTATTTAACAAATGGAATTTTATTTAGGTCGTGTTTTAACAACACAAGACCGGAGCCAATAAGTATAATATCTTTAATAATAAAAC  >  1/1809427‑1809825                                                                                                                                                                                                                                                      |                                                                                                                                                          gCCGTTTTCAAGTGTAATTGTTTTTGGAAAACCGCAACTAGGTACCCCAATGATGATTGAATCCCCAACCCTAGCTATTGATTTACCATTAAAAGCTTTAGTATGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTtatttatt                                                                                                                                                      >  1:398090/1‑251 (MQ=255)  ccGTTTTCAAGTGTAATTGTTTTTGGAAAACCGCAACTAGGTACCCCAATGATGATTGAATCCCCAACCCTAGCTATTGATTTACCATTAAAAGCTTTAGTATGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTtatttatt                                                                                                                                                      <  1:543366/250‑1 (MQ=255)    gTTTTCAAGTGTAATTGTTTTTGGAAAACCGCAACTAGGTACCCCAATGATGATTGAATCCCCAACCCTAGCTATTGATTTACCATTAAAAGCTTTAGTATGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTtattat                                                                                                                                                    >  1:781019/1‑250 (MQ=255)        tCAAGTGTAATTGTTTTTGGAAAACCGCAACTAGGTACCCCAATGATGATTGAATCCCCAACCCTAGCTATTGATTTACCATTAAAAGCTTTAGTATGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTTATTATAGTAc                                                                                                                                               <  1:901766/251‑1 (MQ=255)                              aaCCGCAACTAGGTACCCCAATGATGATTGAATCCCCAACCCTAGCTATTGATTTACCATTAAAAGCTTTAGTATGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTtattat                                                                                                                                                    >  1:294511/1‑224 (MQ=255)                                             ccccAATGATGATTGAATCCCCAACCCTAGCTATTGATTTACCATTAAAAGCTTTAGTATGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTtattat                                                                                                                                                    >  1:126476/1‑209 (MQ=255)                                             ccccAATGATGATTGAATCCCCAACCCTAGCTATTGATTTACCATTAAAAGCTTTAGTATGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTTATTATAGTAc                                                                                                                                               >  1:411021/1‑214 (MQ=255)                                                                cccAACCCTAGCTTTTGATTTACCATTAAAAGCTTTAGTATGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTTATTATAGTACTTTATAAATACAAAAATAAGTTCGCCTAAATTGAGGCGAACTTATTTATTACCGAc                                                                                       <  1:553428/251‑1 (MQ=255)                                                                    aCCCTAGCTATTGATTTACCATTAAAAGCTTTAGTATGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTTATTATAGTACTTTATAAATACAAAAATAAGTTCGCCTAAATTGAGGCGAACTTATTTATTACCGACTAtt                                                                                   >  1:703797/1‑251 (MQ=255)                                                                                        aTTAAAAGCTTTAGTATGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTTATTATAGTACTTTATaa                                                                                                                                        <  1:680840/178‑1 (MQ=255)                                                                                               gCTTTAGTATGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTTATTATAGTACTTTATAAATACAAAAATAAGTTCGCCTAAATTGAGGCGAACTTATTTATTACCGACTATTTAACAAATGGAATtttat                                                                 <  1:302115/242‑1 (MQ=255)                                                                                               gCTTTAGTATGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTTATTATAGTACTTTATAAATACAAAAATAAGTTCGCCTAAATTGAGGCGAACTTATTTATTACCGACTATTTAACAAATGGAATTTTATTTAGGTCgt                                                        >  1:770850/1‑251 (MQ=255)                                                                                                   tAGTATGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTTATTATAGTACTTTATAAATCCAAAAATAAGTTCGCCTAAATTGAGGCGAACttattt                                                                                                >  1:437461/1‑207 (MQ=255)                                                                                                     gTATGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTTATTATAGTACTTTATAAATACAAAAATAAGTTCGCCTAAATTGAGGCGAACTTATTTATTACCGACTATTTAACAAATGGAATtttatt                                                                <  1:93795/237‑1 (MQ=255)                                                                                                       aTGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTTATTATAGTACTTTATAAATACAAAAATAAGTTCGCCTAAATTGAGGCGAACTTATTTATTACCGACTATTTAACAAATGGAATTTTATTTAGGTCGTGTTTTaac                                                <  1:171345/251‑1 (MQ=255)                                                                                                        tGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTTATTATAGTACTTTATAAATAc                                                                                                                                    <  1:602493/166‑1 (MQ=255)                                                                                                                       ggTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTTATTATAGTACTTTATAAATACAAAAATAAGTTCGCCTAAATTGAGGCGAACTTATTTATTACCGACTATTTAACAAATGGAATTTTATTTAGGTCGTGTTTTAACAACACAAGACCGGAg                                 >  1:879326/1‑250 (MQ=255)                                                                                                                                ttCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTtattat                                                                                                                                                    <  1:720419/126‑1 (MQ=255)                                                                                                                                                gAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTTATTATAGTACTTTATAAATACAAAAATAAGTTCGCCTAAATTGAGGCGAACTTATTTATTACCGACTATTTAACAAATGGAATTTTATTTAGGTCGTGTTTTAACAACACAAGACCGGAGCCAATAAGTATAatat                 >  1:706042/1‑241 (MQ=255)                                                                                                                                                      agaTTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTATTTATTATAGTACTTTATAAATACAAAAATAAGTTCGCCTAAATTGAGGCGAACTTATTTATTACCGACTATTTAACAAATGGAATTTTATTTAGGTCGTGTTTTAACAACACAAGACCGGAGCCAATAAGTATAATATCTTTAATAATAAAAc  <  1:29432/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                          GCCGTTTTCAAGTGTAATTGTTTTTGGAAAACCGCAACTAGGTACCCCAATGATGATTGAATCCCCAACCCTAGCTATTGATTTACCATTAAAAGCTTTAGTATGGGAGAATAGCCAAGGTGAAATATTCGTAAGTCTAAATAGAAATGAGATTATAGCAGAAAAACATAATGTCACGGCAGGGAATGTAGAAAAAATGAATAAACTGGTGAAGTTAATAACTGAAAGTGTTACTGAATAAGTTACTTATTATAGTACTTTATAAATACAAAAATAAGTTCGCCTAAATTGAGGCGAACTTATTTATTACCGACTATTTAACAAATGGAATTTTATTTAGGTCGTGTTTTAACAACACAAGACCGGAGCCAATAAGTATAATATCTTTAATAATAAAAC  >  1/1809427‑1809825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑