Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,808,520	G→C	H150D (CAT→GAT)	unknown ←	probable glucanotransferase (endo alpha‑1,4 polygalactosaminidase related protein)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,808,520	0	G	C	100.0%	129.0 / NA	43	H150D (CAT→GAT)	unknown	probable glucanotransferase (endo alpha‑1,4 polygalactosaminidase related protein)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (23/20);  total (23/20)

TCTGCTGGATTCCATATTTTTTTTTATTAAAATCAATAGTGGTAAAAACACTTTCTTGGTTAATACCATCAATTAAAACGGTATTTTGATTAATACATTTTTTTACAAAAAGATCCCCTCCATTAATAATAAGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATGATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGAAAATGACTATAATACGGGCGGTGTTTCTCAATTGCTCCAATATTAATATACCCATACACAGTTTTCCCTGAAGC  >  1/1808316‑1808754                                                                                                                                                                                                             |                                                                                                                                                                                                                                           tCTGTTGGTTTCCATA‑‑TTTTTTTATTAAAATCAATAGTGGTAAAAACACTTTCTTGGTTAATACCATCAATTAAAACGGTATTTTGATTAATACATTTTTTTACAAAAAGATCCCCTCCATTAATAATAAGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATc                                                                                                                                                                                            <  1:606918/251‑1 (MQ=255)  ctgctgGATTCCATA‑TTTTTTTTATTAAAATCAATAGTGGTAAAAACACTTTCTTGGTTAATACCATCAATTAAAACGGTATTTTGATTAATACATTTTTTTACAAAAAGATCCCCTCCATTAATAATAAGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACa                                                                                                                                                                                                                                  <  1:656712/213‑1 (MQ=255)  ctgctgGATTCCATA‑TTTTTTTTATTAAAATCAATAGTGGTAAAAACACTTTCTTGGTTAATACCATCAATTAAAACGGTATTTTGATTAATACATTTTTTTACAAAAAGATCCCCTCCATTAATAATAAGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATc                                                                                                                                                                                            <  1:314439/251‑1 (MQ=255)         aTTCCATA‑TTTTTTTTATTAAAATCAATAGTGGTAAAAACACTTTCTTGGTTAATACCATCAATTAAAACGGTATTTTGATTAATACATTTTTTTACAAAAAGATCCCCTCCATTAATAATAAGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATa                                                                                                                                                                                      >  1:127710/1‑250 (MQ=255)                                    aTAGTGGTAAAAACACTTTCTTGGTTAATACCATCAATTAAAACGGTATTTTGATTAATACATTTTTTTACAAAAAGATCCCCTCCATTAATAATAAGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACttt                                                                                                                                                                                  >  1:66624/1‑228 (MQ=255)                                         ggTAAAAACACTTTCTTGGTTAATACCATCAATTAAAACGGTATTTTGATTAATACATTTTTTTACAAAAAGATCCCCTCCATTAATAATAAGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCt                                                                                                                                                                            >  1:324900/1‑229 (MQ=255)                                          gTAAAAACACTTTCTTGGTTAATACCATCAATTAAAACGGTATTTTGATTAATACATTTTTTTACAAAAAGATCCCCTCCATTAATAATAAGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATcc                                                                                                                                                                                                       <  1:272385/201‑1 (MQ=255)                                                 cacTTTCTTGGTTAATACCATCAATTAAAACGGTATTTTGATTAATACATTTTTTTACAAAAAGATCCCCTCCATTAATAATAAGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTa                                                                                                                                                                                                                         >  1:557292/1‑176 (MQ=255)                                                                       aaTTAAAACGGTATTTTGATTAATACATTTTTTTACAAAAAGATCCCCTCCATTAATAATAAGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCAt                                                                                                                         >  1:596878/1‑250 (MQ=255)                                                                                 gTATTTTGATTAATACATTTTTTTACAAAAAGATCCCCTCCATTAATAATAAGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCgg                                                                                                              >  1:130351/1‑251 (MQ=255)                                                                                   aTTTTGATTAATACATTTTTTTACAAAAAGATCCCCTCCATTAATAATAAGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAAc                                                                                                                                                                                                                                   >  1:567330/1‑132 (MQ=255)                                                                                             aTACATTTTTTTACAAAAAGATCCCCTCCATTAATAATAAGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAAcccc                                                                                                                                                                                                 <  1:786031/156‑1 (MQ=255)                                                                                                                     ccTCCATTAATAATAAGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAg                                                                                                 >  1:166282/1‑228 (MQ=255)                                                                                                                        ccATTAATAATAAGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATcc                                                                                                                <  1:522653/210‑1 (MQ=255)                                                                                                                             aataataaGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCCCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGgaga                                                                                                                                         <  1:459352/180‑1 (MQ=255)                                                                                                                                aataaGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGaaaa                                                                         <  1:228588/241‑1 (MQ=255)                                                                                                                                        gAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAAc                                                                                                                                                         >  1:103323/1‑153 (MQ=255)                                                                                                                                        gAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCgg                                                                                                                                            <  1:755491/166‑1 (MQ=255)                                                                                                                                        gAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAAccc                                                                                                                           >  1:128980/1‑183 (MQ=255)                                                                                                                                            attatAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAAccc                                                                                                                                                                                                  <  1:861151/108‑1 (MQ=255)                                                                                                                                            attatAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGgag                                                                                                                                          >  1:73123/1‑164 (MQ=255)                                                                                                                                            attatAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGc                                                                                        >  1:822263/1‑214 (MQ=255)                                                                                                                                            attatAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGAAAATGACTATAATAc                                                             <  1:517684/241‑1 (MQ=255)                                                                                                                                             ttatAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCa                                                                                                                                                  >  1:824087/1‑155 (MQ=255)                                                                                                                                             ttatAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGAAAATGACTATAATACGGGCGGTGttt                                                  >  1:515856/1‑251 (MQ=255)                                                                                                                                               ataataTCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATc                                                                                                                 >  1:881238/1‑186 (MQ=255)                                                                                                                                                   tatCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCAtaataa                                                                                                                                                                                                                                     <  1:180044/66‑1 (MQ=255)                                                                                                                                                    atCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATc                                                                                                                                                                                            <  1:472050/106‑1 (MQ=255)                                                                                                                                                    atCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGAAAATGACTATAATACGGGCGGTGTTtctc                                               <  1:184795/247‑1 (MQ=255)                                                                                                                                                    atCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGAAAATGACTATAATACGGGCGGTGTTTCTCAAtt                                           >  1:743079/1‑251 (MQ=255)                                                                                                                                                               gTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGAAAATGACTATAATACGGGCGGTGTTTCTCAATTGCTCCAata                                  >  1:848116/1‑249 (MQ=255)                                                                                                                                                                   aaaTTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATAc                                                                                             <  1:585326/186‑1 (MQ=255)                                                                                                                                                                        gTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGAAAATGACTATAATACGGGCGGTGTTTCTCAATTGCTCCAATATTAATATAcc                        >  1:436216/1‑250 (MQ=255)                                                                                                                                                                         ttACAAGCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCAt                                                                                                                                                                                                                           >  1:803807/1‑54 (MQ=255)                                                                                                                                                                               gCCCTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGAAAATGACTATAATACGGGCGGTGTTTCTCAATTGCTCCAATATTAatat                           <  1:145177/240‑1 (MQ=255)                                                                                                                                                                                cccTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGAAAATGACTATAATAc                                                             <  1:153183/205‑1 (MQ=255)                                                                                                                                                                                cccTTGAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGAAAATGACTATAATACGGGCGGTGTTTCTCAATTGCTCCAATATTAATATACCCATACACAg               >  1:242664/1‑251 (MQ=255)                                                                                                                                                                                     gAAATATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGc                                                                                                                      >  1:293666/1‑143 (MQ=255)                                                                                                                                                                                      aaaTATCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGAAAATGACTATAATACGGGCGGTGTTtctc                                               >  1:500088/1‑213 (MQ=255)                                                                                                                                                                                        atatCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGAAAATGACTATAATACGGGCGGTGTTTCTCAATTGCTCCAATATTAATATACCCATACACAGTTTTcc         >  1:408610/1‑249 (MQ=255)                                                                                                                                                                                          atCTCATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGAAAATGACTATAATACGGGCGGTGTTTCTCAATTGCTCCAATATTAATATAcc                        <  1:379565/232‑1 (MQ=255)                                                                                                                                                                                            ctcATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGAAAATGACTATAATACGGGCGGTGTTTCTCAATTGCTCCAATATTAATATACCCATACACAGTTTTCCCTGAAg   <  1:795022/251‑1 (MQ=255)                                                                                                                                                                                             tcATTTGTTTGATAATCATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGAAAATGACTATAATACGGGCGGTGTTTCTCAATTGCTCCAATATTAATATACCCATACACAGTTTTCCCTGAAGc  <  1:48697/251‑1 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                                                                                           TCTGCTGGATTCCATATTTTTTTTTATTAAAATCAATAGTGGTAAAAACACTTTCTTGGTTAATACCATCAATTAAAACGGTATTTTGATTAATACATTTTTTTACAAAAAGATCCCCTCCATTAATAATAAGTGGAATATTATAATATCGAAGCCCAGTCAAAATTGTTACAAGCCCTTGAAATATCTCATTTGTTTGATAATGATAATAAACATCTGCATTATCAAGAAAAAAACCATCCAACCCCATATCAGCATACTTTTTTCCTAGCTCATTAACGATAAAACGCTGCCATACCGGAGAGGATACATCAACCCATCGCTCATCCGGCCAAGTTTCATAGATACCAAGCATGACTGACTGAAAATGACTATAATACGGGCGGTGTTTCTCAATTGCTCCAATATTAATATACCCATACACAGTTTTCCCTGAAGC  >  1/1808316‑1808754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑